UniProtKB - O88783 (FA5_MOUSE)
Protein
Coagulation factor V
Gene
F5
Organism
Mus musculus (Mouse)
Status
Functioni
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.
Activity regulationi
Inhibited by SERPINA5.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 138 | CalciumBy similarity | 1 | |
Metal bindingi | 139 | CalciumBy similarity | 1 | |
Metal bindingi | 1802 | CopperBy similarity | 1 | |
Metal bindingi | 1804 | CopperBy similarity | 1 |
GO - Molecular functioni
- copper ion binding Source: InterPro
GO - Biological processi
- blood circulation Source: MGI
- blood coagulation Source: MGI
Keywordsi
Biological process | Blood coagulation, Hemostasis |
Ligand | Calcium, Copper, Metal-binding |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-204005, COPII-mediated vesicle transport R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-5694530, Cargo concentration in the ER R-MMU-8957275, Post-translational protein phosphorylation |
Names & Taxonomyi
Protein namesi | Recommended name: Coagulation factor VAlternative name(s): Activated protein C cofactor Cleaved into the following 2 chains: |
Gene namesi | Name:F5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:88382, F5 |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Extracellular region or secreted
- extracellular region Source: MGI
- extracellular space Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Other locations
- platelet alpha granule Source: MGI
Keywords - Cellular componenti
SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 333 | R → Q: Does not affect pro-coagulant function. Partially resistant to inactivation by activated protein C. 1 Publication | 1 | |
Mutagenesisi | 532 | R → Q: Does not affect pro-coagulant function. Partially resistant to inactivation by activated protein C. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | Sequence analysisAdd BLAST | 19 | |
ChainiPRO_0000358718 | 20 – 2183 | Coagulation factor VAdd BLAST | 2164 | |
ChainiPRO_0000358719 | 20 – 736 | Coagulation factor V heavy chainBy similarityAdd BLAST | 717 | |
PropeptideiPRO_0000358720 | 737 – 1533 | Activation peptide (connecting region)By similarityAdd BLAST | 797 | |
ChainiPRO_0000358721 | 1534 – 2183 | Coagulation factor V light chainBy similarityAdd BLAST | 650 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 176 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 238 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 381 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 638 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 692 | SulfotyrosineSequence analysis | 1 | |
Modified residuei | 725 | SulfotyrosineSequence analysis | 1 | |
Glycosylationi | 841 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Modified residuei | 903 | PhosphoserineCombined sources | 1 | |
Glycosylationi | 959 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 972 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1438 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 1811 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 1866 ↔ 2020 | PROSITE-ProRule annotation | ||
Disulfide bondi | 2025 ↔ 2180 | PROSITE-ProRule annotation |
Post-translational modificationi
Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).By similarity
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.By similarity
Activated protein C inactivates factor V and factor Va by proteolytic degradation.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 333 – 334 | Cleavage; by activated protein CBy similarity | 2 | |
Sitei | 532 – 533 | Cleavage; by activated protein CBy similarity | 2 | |
Sitei | 706 – 707 | Cleavage; by activated protein CBy similarity | 2 | |
Sitei | 736 – 737 | Cleavage; by thrombinBy similarity | 2 | |
Sitei | 1004 – 1005 | Cleavage; by activated protein CBy similarity | 2 | |
Sitei | 1029 – 1030 | Cleavage; by thrombinBy similarity | 2 | |
Sitei | 1533 – 1534 | Cleavage; by thrombinBy similarity | 2 |
Keywords - PTMi
Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation, ZymogenProteomic databases
MaxQBi | O88783 |
PaxDbi | O88783 |
PeptideAtlasi | O88783 |
PRIDEi | O88783 |
PTM databases
GlyGeni | O88783, 8 sites |
iPTMneti | O88783 |
PhosphoSitePlusi | O88783 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026579, Expressed in blood and 145 other tissues |
Genevisiblei | O88783, MM |
Interactioni
Subunit structurei
Factor Va, the activated form of factor V, is composed of a heavy chain and a light chain, non-covalently bound. The interaction between the two chains is calcium-dependent. Forms heterodimer with SERPINA5 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 199574, 3 interactors |
IntActi | O88783, 2 interactors |
MINTi | O88783 |
STRINGi | 10090.ENSMUSP00000083204 |
Miscellaneous databases
RNActi | O88783, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 328 | F5/8 type A 1Add BLAST | 299 | |
Domaini | 30 – 192 | Plastocyanin-like 1Add BLAST | 163 | |
Domaini | 202 – 328 | Plastocyanin-like 2Add BLAST | 127 | |
Domaini | 347 – 682 | F5/8 type A 2Add BLAST | 336 | |
Domaini | 347 – 524 | Plastocyanin-like 3Add BLAST | 178 | |
Domaini | 534 – 682 | Plastocyanin-like 4Add BLAST | 149 | |
Repeati | 892 – 911 | 1-1Add BLAST | 20 | |
Repeati | 1175 – 1183 | 2-1 | 9 | |
Repeati | 1184 – 1192 | 2-2 | 9 | |
Repeati | 1193 – 1201 | 2-3 | 9 | |
Repeati | 1202 – 1210 | 2-4 | 9 | |
Repeati | 1211 – 1219 | 2-5 | 9 | |
Repeati | 1220 – 1228 | 2-6 | 9 | |
Repeati | 1229 – 1237 | 2-7 | 9 | |
Repeati | 1238 – 1246 | 2-8 | 9 | |
Repeati | 1247 – 1255 | 2-9 | 9 | |
Repeati | 1256 – 1264 | 2-10 | 9 | |
Repeati | 1265 – 1273 | 2-11 | 9 | |
Repeati | 1274 – 1282 | 2-12 | 9 | |
Repeati | 1283 – 1291 | 2-13 | 9 | |
Repeati | 1292 – 1299 | 2-14 | 8 | |
Repeati | 1300 – 1308 | 2-15 | 9 | |
Repeati | 1309 – 1316 | 2-16 | 8 | |
Repeati | 1317 – 1325 | 2-17 | 9 | |
Repeati | 1326 – 1334 | 2-18 | 9 | |
Repeati | 1335 – 1341 | 2-19 | 7 | |
Repeati | 1342 – 1350 | 2-20 | 9 | |
Repeati | 1351 – 1359 | 2-21 | 9 | |
Repeati | 1360 – 1368 | 2-22 | 9 | |
Repeati | 1369 – 1377 | 2-23 | 9 | |
Repeati | 1378 – 1386 | 2-24 | 9 | |
Repeati | 1387 – 1395 | 2-25 | 9 | |
Repeati | 1396 – 1404 | 2-26 | 9 | |
Repeati | 1405 – 1413 | 2-27 | 9 | |
Repeati | 1414 – 1422 | 2-28 | 9 | |
Repeati | 1423 – 1431 | 2-29 | 9 | |
Repeati | 1432 – 1440 | 2-30 | 9 | |
Repeati | 1441 – 1449 | 2-31 | 9 | |
Repeati | 1452 – 1461 | 2-32 | 10 | |
Domaini | 1538 – 1866 | F5/8 type A 3Add BLAST | 329 | |
Domaini | 1538 – 1711 | Plastocyanin-like 5Add BLAST | 174 | |
Domaini | 1721 – 1866 | Plastocyanin-like 6Add BLAST | 146 | |
Domaini | 1866 – 2020 | F5/8 type C 1PROSITE-ProRule annotationAdd BLAST | 155 | |
Domaini | 2025 – 2180 | F5/8 type C 2PROSITE-ProRule annotationAdd BLAST | 156 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 691 – 1533 | BBy similarityAdd BLAST | 843 | |
Regioni | 892 – 908 | 1 X 17 AA tandem repeatsAdd BLAST | 17 | |
Regioni | 1175 – 1461 | 32 X 9 AA approximate tandem repeats of [TNP]-L-S-P-D-L-S-Q-TAdd BLAST | 287 |
Domaini
Domain B contains 32 X 9 AA tandem repeats, and 1 X 17 AA repeats.
Sequence similaritiesi
Belongs to the multicopper oxidase family.Curated
Keywords - Domaini
Repeat, SignalPhylogenomic databases
eggNOGi | ENOG502QSUG, Eukaryota |
GeneTreei | ENSGT00940000158556 |
HOGENOMi | CLU_000948_0_0_1 |
InParanoidi | O88783 |
OMAi | GDYWEPS |
OrthoDBi | 454773at2759 |
PhylomeDBi | O88783 |
TreeFami | TF329807 |
Family and domain databases
CDDi | cd00057, FA58C, 2 hits |
Gene3Di | 2.60.120.260, 2 hits 2.60.40.420, 5 hits |
InterProi | View protein in InterPro IPR011707, Cu-oxidase_3 IPR033138, Cu_oxidase_CS IPR008972, Cupredoxin IPR000421, FA58C IPR024715, Factor_5/8-like IPR008979, Galactose-bd-like_sf |
Pfami | View protein in Pfam PF07732, Cu-oxidase_3, 3 hits PF00754, F5_F8_type_C, 2 hits |
PIRSFi | PIRSF000354, Factors_V_VIII, 1 hit |
SMARTi | View protein in SMART SM00231, FA58C, 2 hits |
SUPFAMi | SSF49503, SSF49503, 6 hits SSF49785, SSF49785, 2 hits |
PROSITEi | View protein in PROSITE PS01285, FA58C_1, 2 hits PS01286, FA58C_2, 2 hits PS50022, FA58C_3, 2 hits PS00079, MULTICOPPER_OXIDASE1, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O88783-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLLVCPCFFL LVVLGTRWAG WGSHQAEAAQ LRQFYVAAQG ILWNYHPEPT
60 70 80 90 100
DPSLNSIPSF KKIVYREYEQ YFKKEKPRSS NSGLLGPTLY AEVGDVIKVH
110 120 130 140 150
FRNKADKPLS IHPQGIKYSK FSEGASYADH TFPAERKDDA VAPGEEYTYE
160 170 180 190 200
WIVSEDSGPT PDDPPCLTHI YYSYENLTQD FNSGLIGPLL ICKKGTLTED
210 220 230 240 250
GTQKMFDKQH VLLFAVFDES KSRSQSPSLM YTINGFVNKT MPDITVCAHD
260 270 280 290 300
HVSWHLIGMS SGPELFSIHF NGQVLEQNQH KVSTVTLVSA TSTTANMTMS
310 320 330 340 350
PEGRWIVSSL IPKHYQAGMQ AYIDIKNCPK KTRSPKTLTR EQRRYMKRWE
360 370 380 390 400
YFIAAEEVIW NYAPVIPANM DKIYRSQHLD NFSNQIGKHY KKVIYRQYEE
410 420 430 440 450
ETFTKRTDNP SIKQSGILGP VIRAQVRDTL KIVFKNMASR PYSIYPHGVT
460 470 480 490 500
FSPYEDGINS SSTSGSHTTI RPVQPGETFT YKWNILEFDE PTENDAQCLT
510 520 530 540 550
RPYYSDVDVT RDIASGLIGL LLICKSRSLD QRGVQRVADI EQQAVFAVFD
560 570 580 590 600
ENKSWYIEDN INKFCENPDE VKRDDPKFYE SNIMSTINGY VPESISTLGF
610 620 630 640 650
CFDDTVQWHF CSVGTHDDIL TIHFTGHSFI YGRRHEDTLT LFPMRGESVT
660 670 680 690 700
VTMDNVGTWM LTTMNSNPKR RNLRLRFRDV KCNRDYDNED SYEIYEPPAP
710 720 730 740 750
TSMTTRRIHD SLENEFGIDN EDDDYQYLLA SSLGIRSFKN SSLNPEENEF
760 770 780 790 800
NLTALALENS SEFISPSTDR VVDSNSSRIL SKIINNNLKD FQRTLPGSGA
810 820 830 840 850
TVAGTLLRNL IGLDENFVLN SSTEHRSSSY HENDMENPQS NITMVYLLPL
860 870 880 890 900
GPKGSGNREQ DKPKTIKTGR PHMMKHRFSW MKAPAGKTGR HSNPKNSYSG
910 920 930 940 950
MKSEEDIPSE LIPLKQKITS KFLNRRWRVA SEKGSYEIIA ANGEDTDVDK
960 970 980 990 1000
LTNSPQNQNI TVPRGESTSH TNTTRKPSDL PTFSGVGHKS PHVRQEEENS
1010 1020 1030 1040 1050
GFQKRQLFIR TRKKKKNKKL ALHSPLSPRG FDPLRGHNHS PFPDRRLLNH
1060 1070 1080 1090 1100
SLLLHKSNET ALSPDLNQTS PSMSTDRSLP DYNQYSKNDT EQMSSSLDLY
1110 1120 1130 1140 1150
QSVPAEEHSP TFPAQDPDQT HSTTDPSYRS SPPELSQGLD YDLSHDFYPD
1160 1170 1180 1190 1200
DIGLTSFFPD QSQKSSFSSD DDQAIPSSDL SLFTISPELD QTIIYPDLDQ
1210 1220 1230 1240 1250
LLLSPEDNQK TSSPDLGQVP LSPDDNQKTS SPDLGQVSLS PDDNQKTSSP
1260 1270 1280 1290 1300
DLGQVPLSLD DNQKTTSPDL GQVPLSPDDN QMITSPDLGQ VPLSSDNQKT
1310 1320 1330 1340 1350
SSPDLGQVPL FPEDNQNYFL DLSQVPLSSD QNQETSSTDL LTLSPDFGQT
1360 1370 1380 1390 1400
VLSPDLDQLP LPSDNSQVTV SPDLSLLTLS PDFNEIILAP DLGQVTLSPD
1410 1420 1430 1440 1450
LIQTNPALNH GHKASSADPD QASYPPDSGQ ASSLPELNRT LPHPDLTHIP
1460 1470 1480 1490 1500
PPSPSPTLNN TSLSRKFNPL VVVGLSRVDG DDVEIVPSEE PERIDEDYAE
1510 1520 1530 1540 1550
DDFVTYNDPY RTDTRTDVNS SRNPDTIAAW YLRGHGGHKK FYYIAAEEIT
1560 1570 1580 1590 1600
WNYAEFAQSE MDHEDTGHTP KDTTYKKVVF RKYLDSTFTS RDPRAEYEEH
1610 1620 1630 1640 1650
LGILGPVIRA EVDDVIQVRF KNLASRPYSL HAHGLSYEKS SEGKTYEDES
1660 1670 1680 1690 1700
PEWFQEDDAV QPNSSYTYVW HATKRSGPEN PGSACRAWAY YSAVNVERDI
1710 1720 1730 1740 1750
HSGLIGPLLI CRKGTLHMER NLPMDMREFV LLFMVFDEKK SWYYEKSKGS
1760 1770 1780 1790 1800
RRIESPEEKN AHKFYAINGM IYNLPGLRMY EQEWVRLHLL NMGGSRDIHV
1810 1820 1830 1840 1850
VHFHGQTLLD NRTKQHQLGV WPLLPGSFKT LEMKASKPGW WLLDTEVGEN
1860 1870 1880 1890 1900
QVAGMQTPFL IIDKECKMPM GLSTGVISDS QIKASEYLTY WEPRLARLNN
1910 1920 1930 1940 1950
AGSYNAWSIE KTALDFPIKP WIQVDMQKEV VVTGIQTQGA KHYLKSCFTT
1960 1970 1980 1990 2000
EFQVAYSSDQ TNWQIFRGKS GKSVMYFTGN SDGSTIKENR LDPPIVARYI
2010 2020 2030 2040 2050
RIHPTKSYNR PTLRLELQGC EVNGCSTPLG LEDGRIQDKQ ITASSFKKSW
2060 2070 2080 2090 2100
WGDYWEPSLA RLNAQGRVNA WQAKANNNKQ WLQVDLLKIK KVTAIVTQGC
2110 2120 2130 2140 2150
KSLSSEMYVK SYSIQYSDQG VAWKPYRQKS SMVDKIFEGN SNTKGHMKNF
2160 2170 2180
FNPPIISRFI RIIPKTWNQS IALRLELFGC DIY
Sequence cautioni
The sequence BAE23393 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U52925 mRNA Translation: AAC99553.1 AK137521 mRNA Translation: BAE23393.1 Different initiation. AK139240 mRNA Translation: BAE23928.1 |
CCDSi | CCDS35754.1 |
PIRi | T42764 |
RefSeqi | NP_032002.1, NM_007976.3 |
Genome annotation databases
Ensembli | ENSMUST00000086040; ENSMUSP00000083204; ENSMUSG00000026579 |
GeneIDi | 14067 |
KEGGi | mmu:14067 |
UCSCi | uc007dic.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U52925 mRNA Translation: AAC99553.1 AK137521 mRNA Translation: BAE23393.1 Different initiation. AK139240 mRNA Translation: BAE23928.1 |
CCDSi | CCDS35754.1 |
PIRi | T42764 |
RefSeqi | NP_032002.1, NM_007976.3 |
3D structure databases
SMRi | O88783 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199574, 3 interactors |
IntActi | O88783, 2 interactors |
MINTi | O88783 |
STRINGi | 10090.ENSMUSP00000083204 |
PTM databases
GlyGeni | O88783, 8 sites |
iPTMneti | O88783 |
PhosphoSitePlusi | O88783 |
Proteomic databases
MaxQBi | O88783 |
PaxDbi | O88783 |
PeptideAtlasi | O88783 |
PRIDEi | O88783 |
Protocols and materials databases
Antibodypediai | 863, 382 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000086040; ENSMUSP00000083204; ENSMUSG00000026579 |
GeneIDi | 14067 |
KEGGi | mmu:14067 |
UCSCi | uc007dic.1, mouse |
Organism-specific databases
CTDi | 2153 |
MGIi | MGI:88382, F5 |
Phylogenomic databases
eggNOGi | ENOG502QSUG, Eukaryota |
GeneTreei | ENSGT00940000158556 |
HOGENOMi | CLU_000948_0_0_1 |
InParanoidi | O88783 |
OMAi | GDYWEPS |
OrthoDBi | 454773at2759 |
PhylomeDBi | O88783 |
TreeFami | TF329807 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation R-MMU-140875, Common Pathway of Fibrin Clot Formation R-MMU-204005, COPII-mediated vesicle transport R-MMU-381426, Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs) R-MMU-5694530, Cargo concentration in the ER R-MMU-8957275, Post-translational protein phosphorylation |
Miscellaneous databases
BioGRID-ORCSi | 14067, 0 hits in 12 CRISPR screens |
ChiTaRSi | F5, mouse |
PROi | PR:O88783 |
RNActi | O88783, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026579, Expressed in blood and 145 other tissues |
Genevisiblei | O88783, MM |
Family and domain databases
CDDi | cd00057, FA58C, 2 hits |
Gene3Di | 2.60.120.260, 2 hits 2.60.40.420, 5 hits |
InterProi | View protein in InterPro IPR011707, Cu-oxidase_3 IPR033138, Cu_oxidase_CS IPR008972, Cupredoxin IPR000421, FA58C IPR024715, Factor_5/8-like IPR008979, Galactose-bd-like_sf |
Pfami | View protein in Pfam PF07732, Cu-oxidase_3, 3 hits PF00754, F5_F8_type_C, 2 hits |
PIRSFi | PIRSF000354, Factors_V_VIII, 1 hit |
SMARTi | View protein in SMART SM00231, FA58C, 2 hits |
SUPFAMi | SSF49503, SSF49503, 6 hits SSF49785, SSF49785, 2 hits |
PROSITEi | View protein in PROSITE PS01285, FA58C_1, 2 hits PS01286, FA58C_2, 2 hits PS50022, FA58C_3, 2 hits PS00079, MULTICOPPER_OXIDASE1, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FA5_MOUSE | |
Accessioni | O88783Primary (citable) accession number: O88783 Secondary accession number(s): Q3UTQ2, Q3UV80 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 16, 2008 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families