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UniProtKB - O88783 (FA5_MOUSE)

Protein

Coagulation factor V

Gene

F5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

Activity regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138CalciumBy similarity1
Metal bindingi139CalciumBy similarity1
Metal bindingi1802CopperBy similarity1
Metal bindingi1804CopperBy similarity1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • blood circulation Source: MGI
  • blood coagulation Source: MGI
View the complete GO annotation on QuickGO ...

Keywordsi

Biological processBlood coagulation, Hemostasis
LigandCalcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-140875 Common Pathway of Fibrin Clot Formation
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-5694530 Cargo concentration in the ER
R-MMU-8957275 Post-translational protein phosphorylation

Names & Taxonomyi

Protein namesi
Recommended name:
Coagulation factor V
Alternative name(s):
Activated protein C cofactor
Cleaved into the following 2 chains:
Coagulation factor V heavy chain
Coagulation factor V light chain
Gene namesi
Name:F5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:88382 F5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi333R → Q: Does not affect pro-coagulant function. Partially resistant to inactivation by activated protein C. 1 Publication1
Mutagenesisi532R → Q: Does not affect pro-coagulant function. Partially resistant to inactivation by activated protein C. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000035871820 – 2183Coagulation factor VAdd BLAST2164
ChainiPRO_000035871920 – 736Coagulation factor V heavy chainBy similarityAdd BLAST717
PropeptideiPRO_0000358720737 – 1533Activation peptide (connecting region)By similarityAdd BLAST797
ChainiPRO_00003587211534 – 2183Coagulation factor V light chainBy similarityAdd BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi238N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi381N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei638PhosphothreonineBy similarity1
Modified residuei692SulfotyrosineSequence analysis1
Modified residuei725SulfotyrosineSequence analysis1
Glycosylationi841N-linked (GlcNAc...) asparagine1 Publication1
Modified residuei903PhosphoserineCombined sources1
Glycosylationi959N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi972N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1438N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi1811N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1866 ↔ 2020PROSITE-ProRule annotation
Disulfide bondi2025 ↔ 2180PROSITE-ProRule annotation

Post-translational modificationi

Thrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).By similarity
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.By similarity
Activated protein C inactivates factor V and factor Va by proteolytic degradation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei333 – 334Cleavage; by activated protein CBy similarity2
Sitei532 – 533Cleavage; by activated protein CBy similarity2
Sitei706 – 707Cleavage; by activated protein CBy similarity2
Sitei736 – 737Cleavage; by thrombinBy similarity2
Sitei1004 – 1005Cleavage; by activated protein CBy similarity2
Sitei1029 – 1030Cleavage; by thrombinBy similarity2
Sitei1533 – 1534Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Sulfation, Zymogen

Proteomic databases

MaxQBiO88783
PaxDbiO88783
PeptideAtlasiO88783
PRIDEiO88783

PTM databases

iPTMnetiO88783
PhosphoSitePlusiO88783

Expressioni

Gene expression databases

BgeeiENSMUSG00000026579 Expressed in 119 organ(s), highest expression level in blood
GenevisibleiO88783 MM

Interactioni

Subunit structurei

Factor Va, the activated form of factor V, is composed of a heavy chain and a light chain, non-covalently bound. The interaction between the two chains is calcium-dependent. Forms heterodimer with SERPINA5 (By similarity).By similarity

Protein-protein interaction databases

IntActiO88783, 2 interactors
MINTiO88783
STRINGi10090.ENSMUSP00000083204

Structurei

3D structure databases

ProteinModelPortaliO88783
SMRiO88783
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 328F5/8 type A 1Add BLAST299
Domaini30 – 192Plastocyanin-like 1Add BLAST163
Domaini202 – 328Plastocyanin-like 2Add BLAST127
Domaini347 – 682F5/8 type A 2Add BLAST336
Domaini347 – 524Plastocyanin-like 3Add BLAST178
Domaini534 – 682Plastocyanin-like 4Add BLAST149
Repeati892 – 9111-1Add BLAST20
Repeati1175 – 11832-19
Repeati1184 – 11922-29
Repeati1193 – 12012-39
Repeati1202 – 12102-49
Repeati1211 – 12192-59
Repeati1220 – 12282-69
Repeati1229 – 12372-79
Repeati1238 – 12462-89
Repeati1247 – 12552-99
Repeati1256 – 12642-109
Repeati1265 – 12732-119
Repeati1274 – 12822-129
Repeati1283 – 12912-139
Repeati1292 – 12992-148
Repeati1300 – 13082-159
Repeati1309 – 13162-168
Repeati1317 – 13252-179
Repeati1326 – 13342-189
Repeati1335 – 13412-197
Repeati1342 – 13502-209
Repeati1351 – 13592-219
Repeati1360 – 13682-229
Repeati1369 – 13772-239
Repeati1378 – 13862-249
Repeati1387 – 13952-259
Repeati1396 – 14042-269
Repeati1405 – 14132-279
Repeati1414 – 14222-289
Repeati1423 – 14312-299
Repeati1432 – 14402-309
Repeati1441 – 14492-319
Repeati1452 – 14612-3210
Domaini1538 – 1866F5/8 type A 3Add BLAST329
Domaini1538 – 1711Plastocyanin-like 5Add BLAST174
Domaini1721 – 1866Plastocyanin-like 6Add BLAST146
Domaini1866 – 2020F5/8 type C 1PROSITE-ProRule annotationAdd BLAST155
Domaini2025 – 2180F5/8 type C 2PROSITE-ProRule annotationAdd BLAST156

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni691 – 1533BBy similarityAdd BLAST843
Regioni892 – 9081 X 17 AA tandem repeatsAdd BLAST17
Regioni1175 – 146132 X 9 AA approximate tandem repeats of [TNP]-L-S-P-D-L-S-Q-TAdd BLAST287

Domaini

Domain B contains 32 X 9 AA tandem repeats, and 1 X 17 AA repeats.

Sequence similaritiesi

Belongs to the multicopper oxidase family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IJ6Y Eukaryota
ENOG4111F6G LUCA
GeneTreeiENSGT00910000143988
HOGENOMiHOG000082542
HOVERGENiHBG005631
InParanoidiO88783
KOiK03902
OMAiTEVGENQ
OrthoDBiEOG091G00QL
PhylomeDBiO88783
TreeFamiTF329807

Family and domain databases

CDDicd00057 FA58C, 2 hits
Gene3Di2.60.120.260, 2 hits
2.60.40.420, 5 hits
InterProiView protein in InterPro
IPR011707 Cu-oxidase_3
IPR033138 Cu_oxidase_CS
IPR008972 Cupredoxin
IPR000421 FA58C
IPR024715 Factor_5/8_like
IPR008979 Galactose-bd-like_sf
PfamiView protein in Pfam
PF07732 Cu-oxidase_3, 3 hits
PF00754 F5_F8_type_C, 2 hits
PIRSFiPIRSF000354 Factors_V_VIII, 1 hit
SMARTiView protein in SMART
SM00231 FA58C, 2 hits
SUPFAMiSSF49503 SSF49503, 6 hits
SSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS01285 FA58C_1, 2 hits
PS01286 FA58C_2, 2 hits
PS50022 FA58C_3, 2 hits
PS00079 MULTICOPPER_OXIDASE1, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88783-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLLVCPCFFL LVVLGTRWAG WGSHQAEAAQ LRQFYVAAQG ILWNYHPEPT 
        60         70         80         90        100
DPSLNSIPSF KKIVYREYEQ YFKKEKPRSS NSGLLGPTLY AEVGDVIKVH 
       110        120        130        140        150
FRNKADKPLS IHPQGIKYSK FSEGASYADH TFPAERKDDA VAPGEEYTYE 
       160        170        180        190        200
WIVSEDSGPT PDDPPCLTHI YYSYENLTQD FNSGLIGPLL ICKKGTLTED 
       210        220        230        240        250
GTQKMFDKQH VLLFAVFDES KSRSQSPSLM YTINGFVNKT MPDITVCAHD 
       260        270        280        290        300
HVSWHLIGMS SGPELFSIHF NGQVLEQNQH KVSTVTLVSA TSTTANMTMS 
       310        320        330        340        350
PEGRWIVSSL IPKHYQAGMQ AYIDIKNCPK KTRSPKTLTR EQRRYMKRWE 
       360        370        380        390        400
YFIAAEEVIW NYAPVIPANM DKIYRSQHLD NFSNQIGKHY KKVIYRQYEE 
       410        420        430        440        450
ETFTKRTDNP SIKQSGILGP VIRAQVRDTL KIVFKNMASR PYSIYPHGVT 
       460        470        480        490        500
FSPYEDGINS SSTSGSHTTI RPVQPGETFT YKWNILEFDE PTENDAQCLT 
       510        520        530        540        550
RPYYSDVDVT RDIASGLIGL LLICKSRSLD QRGVQRVADI EQQAVFAVFD 
       560        570        580        590        600
ENKSWYIEDN INKFCENPDE VKRDDPKFYE SNIMSTINGY VPESISTLGF 
       610        620        630        640        650
CFDDTVQWHF CSVGTHDDIL TIHFTGHSFI YGRRHEDTLT LFPMRGESVT 
       660        670        680        690        700
VTMDNVGTWM LTTMNSNPKR RNLRLRFRDV KCNRDYDNED SYEIYEPPAP 
       710        720        730        740        750
TSMTTRRIHD SLENEFGIDN EDDDYQYLLA SSLGIRSFKN SSLNPEENEF 
       760        770        780        790        800
NLTALALENS SEFISPSTDR VVDSNSSRIL SKIINNNLKD FQRTLPGSGA 
       810        820        830        840        850
TVAGTLLRNL IGLDENFVLN SSTEHRSSSY HENDMENPQS NITMVYLLPL 
       860        870        880        890        900
GPKGSGNREQ DKPKTIKTGR PHMMKHRFSW MKAPAGKTGR HSNPKNSYSG 
       910        920        930        940        950
MKSEEDIPSE LIPLKQKITS KFLNRRWRVA SEKGSYEIIA ANGEDTDVDK 
       960        970        980        990       1000
LTNSPQNQNI TVPRGESTSH TNTTRKPSDL PTFSGVGHKS PHVRQEEENS 
      1010       1020       1030       1040       1050
GFQKRQLFIR TRKKKKNKKL ALHSPLSPRG FDPLRGHNHS PFPDRRLLNH 
      1060       1070       1080       1090       1100
SLLLHKSNET ALSPDLNQTS PSMSTDRSLP DYNQYSKNDT EQMSSSLDLY 
      1110       1120       1130       1140       1150
QSVPAEEHSP TFPAQDPDQT HSTTDPSYRS SPPELSQGLD YDLSHDFYPD 
      1160       1170       1180       1190       1200
DIGLTSFFPD QSQKSSFSSD DDQAIPSSDL SLFTISPELD QTIIYPDLDQ 
      1210       1220       1230       1240       1250
LLLSPEDNQK TSSPDLGQVP LSPDDNQKTS SPDLGQVSLS PDDNQKTSSP 
      1260       1270       1280       1290       1300
DLGQVPLSLD DNQKTTSPDL GQVPLSPDDN QMITSPDLGQ VPLSSDNQKT 
      1310       1320       1330       1340       1350
SSPDLGQVPL FPEDNQNYFL DLSQVPLSSD QNQETSSTDL LTLSPDFGQT 
      1360       1370       1380       1390       1400
VLSPDLDQLP LPSDNSQVTV SPDLSLLTLS PDFNEIILAP DLGQVTLSPD 
      1410       1420       1430       1440       1450
LIQTNPALNH GHKASSADPD QASYPPDSGQ ASSLPELNRT LPHPDLTHIP 
      1460       1470       1480       1490       1500
PPSPSPTLNN TSLSRKFNPL VVVGLSRVDG DDVEIVPSEE PERIDEDYAE 
      1510       1520       1530       1540       1550
DDFVTYNDPY RTDTRTDVNS SRNPDTIAAW YLRGHGGHKK FYYIAAEEIT 
      1560       1570       1580       1590       1600
WNYAEFAQSE MDHEDTGHTP KDTTYKKVVF RKYLDSTFTS RDPRAEYEEH 
      1610       1620       1630       1640       1650
LGILGPVIRA EVDDVIQVRF KNLASRPYSL HAHGLSYEKS SEGKTYEDES 
      1660       1670       1680       1690       1700
PEWFQEDDAV QPNSSYTYVW HATKRSGPEN PGSACRAWAY YSAVNVERDI 
      1710       1720       1730       1740       1750
HSGLIGPLLI CRKGTLHMER NLPMDMREFV LLFMVFDEKK SWYYEKSKGS 
      1760       1770       1780       1790       1800
RRIESPEEKN AHKFYAINGM IYNLPGLRMY EQEWVRLHLL NMGGSRDIHV 
      1810       1820       1830       1840       1850
VHFHGQTLLD NRTKQHQLGV WPLLPGSFKT LEMKASKPGW WLLDTEVGEN 
      1860       1870       1880       1890       1900
QVAGMQTPFL IIDKECKMPM GLSTGVISDS QIKASEYLTY WEPRLARLNN 
      1910       1920       1930       1940       1950
AGSYNAWSIE KTALDFPIKP WIQVDMQKEV VVTGIQTQGA KHYLKSCFTT 
      1960       1970       1980       1990       2000
EFQVAYSSDQ TNWQIFRGKS GKSVMYFTGN SDGSTIKENR LDPPIVARYI 
      2010       2020       2030       2040       2050
RIHPTKSYNR PTLRLELQGC EVNGCSTPLG LEDGRIQDKQ ITASSFKKSW 
      2060       2070       2080       2090       2100
WGDYWEPSLA RLNAQGRVNA WQAKANNNKQ WLQVDLLKIK KVTAIVTQGC 
      2110       2120       2130       2140       2150
KSLSSEMYVK SYSIQYSDQG VAWKPYRQKS SMVDKIFEGN SNTKGHMKNF 
      2160       2170       2180 
FNPPIISRFI RIIPKTWNQS IALRLELFGC DIY
Length:2,183
Mass (Da):247,230
Last modified:November 1, 1998 - v1
Checksum:iBF0A8AA723F60317
GO

Sequence cautioni

The sequence BAE23393 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52925 mRNA Translation: AAC99553.1
AK137521 mRNA Translation: BAE23393.1 Different initiation.
AK139240 mRNA Translation: BAE23928.1
CCDSiCCDS35754.1
PIRiT42764
RefSeqiNP_032002.1, NM_007976.3
UniGeneiMm.12900

Genome annotation databases

EnsembliENSMUST00000086040; ENSMUSP00000083204; ENSMUSG00000026579
GeneIDi14067
KEGGimmu:14067
UCSCiuc007dic.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U52925 mRNA Translation: AAC99553.1
AK137521 mRNA Translation: BAE23393.1 Different initiation.
AK139240 mRNA Translation: BAE23928.1
CCDSiCCDS35754.1
PIRiT42764
RefSeqiNP_032002.1, NM_007976.3
UniGeneiMm.12900

3D structure databases

ProteinModelPortaliO88783
SMRiO88783
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88783, 2 interactors
MINTiO88783
STRINGi10090.ENSMUSP00000083204

PTM databases

iPTMnetiO88783
PhosphoSitePlusiO88783

Proteomic databases

MaxQBiO88783
PaxDbiO88783
PeptideAtlasiO88783
PRIDEiO88783

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000086040; ENSMUSP00000083204; ENSMUSG00000026579
GeneIDi14067
KEGGimmu:14067
UCSCiuc007dic.1 mouse

Organism-specific databases

CTDi2153
MGIiMGI:88382 F5

Phylogenomic databases

eggNOGiENOG410IJ6Y Eukaryota
ENOG4111F6G LUCA
GeneTreeiENSGT00910000143988
HOGENOMiHOG000082542
HOVERGENiHBG005631
InParanoidiO88783
KOiK03902
OMAiTEVGENQ
OrthoDBiEOG091G00QL
PhylomeDBiO88783
TreeFamiTF329807

Enzyme and pathway databases

ReactomeiR-MMU-114608 Platelet degranulation
R-MMU-140875 Common Pathway of Fibrin Clot Formation
R-MMU-204005 COPII-mediated vesicle transport
R-MMU-381426 Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs)
R-MMU-5694530 Cargo concentration in the ER
R-MMU-8957275 Post-translational protein phosphorylation

Miscellaneous databases

PROiPR:O88783
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026579 Expressed in 119 organ(s), highest expression level in blood
GenevisibleiO88783 MM

Family and domain databases

CDDicd00057 FA58C, 2 hits
Gene3Di2.60.120.260, 2 hits
2.60.40.420, 5 hits
InterProiView protein in InterPro
IPR011707 Cu-oxidase_3
IPR033138 Cu_oxidase_CS
IPR008972 Cupredoxin
IPR000421 FA58C
IPR024715 Factor_5/8_like
IPR008979 Galactose-bd-like_sf
PfamiView protein in Pfam
PF07732 Cu-oxidase_3, 3 hits
PF00754 F5_F8_type_C, 2 hits
PIRSFiPIRSF000354 Factors_V_VIII, 1 hit
SMARTiView protein in SMART
SM00231 FA58C, 2 hits
SUPFAMiSSF49503 SSF49503, 6 hits
SSF49785 SSF49785, 2 hits
PROSITEiView protein in PROSITE
PS01285 FA58C_1, 2 hits
PS01286 FA58C_2, 2 hits
PS50022 FA58C_3, 2 hits
PS00079 MULTICOPPER_OXIDASE1, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiFA5_MOUSE
AccessioniPrimary (citable) accession number: O88783
Secondary accession number(s): Q3UTQ2, Q3UV80
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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