Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Presenilin-2

Gene

Psen2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei263By similarity1
Active sitei366By similarity1

GO - Molecular functioni

  • aspartic endopeptidase activity, intramembrane cleaving Source: InterPro
  • endopeptidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease
Biological processNotch signaling pathway

Enzyme and pathway databases

ReactomeiR-RNO-1251985 Nuclear signaling by ERBB4
R-RNO-193692 Regulated proteolysis of p75NTR
R-RNO-205043 NRIF signals cell death from the nucleus
R-RNO-3928665 EPH-ephrin mediated repulsion of cells
R-RNO-9013507 NOTCH3 Activation and Transmission of Signal to the Nucleus
R-RNO-9017802 Noncanonical activation of NOTCH3

Protein family/group databases

MEROPSiA22.002

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilin-2 (EC:3.4.23.-)
Short name:
PS-2
Cleaved into the following 2 chains:
Gene namesi
Name:Psen2
Synonyms:Ps2, Psnl2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi621060 Psen2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 87CytoplasmicSequence analysisAdd BLAST87
Transmembranei88 – 106HelicalSequence analysisAdd BLAST19
Topological domaini107 – 138LumenalSequence analysisAdd BLAST32
Transmembranei139 – 159HelicalSequence analysisAdd BLAST21
Topological domaini160 – 166CytoplasmicSequence analysis7
Transmembranei167 – 187HelicalSequence analysisAdd BLAST21
Topological domaini188 – 200LumenalSequence analysisAdd BLAST13
Transmembranei201 – 221HelicalSequence analysisAdd BLAST21
Topological domaini222 – 223CytoplasmicSequence analysis2
Transmembranei224 – 244HelicalSequence analysisAdd BLAST21
Topological domaini245 – 249LumenalSequence analysis5
Transmembranei250 – 270HelicalSequence analysisAdd BLAST21
Topological domaini271 – 358CytoplasmicSequence analysisAdd BLAST88
Transmembranei359 – 379HelicalSequence analysisAdd BLAST21
Topological domaini380 – 388LumenalSequence analysis9
Transmembranei389 – 409HelicalSequence analysisAdd BLAST21
Topological domaini410 – 413CytoplasmicSequence analysis4
Intramembranei414 – 434HelicalSequence analysisAdd BLAST21
Topological domaini435 – 448CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000256091 – 297Presenilin-2 NTF subunitBy similarityAdd BLAST297
ChainiPRO_0000025610298 – 448Presenilin-2 CTF subunitBy similarityAdd BLAST151

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei22PhosphoserineBy similarity1
Modified residuei25PhosphoserineBy similarity1
Modified residuei30PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on serine residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88777
PRIDEiO88777

PTM databases

iPTMnetiO88777
PhosphoSitePlusiO88777

Expressioni

Gene expression databases

BgeeiENSRNOG00000002879 Expressed in 10 organ(s), highest expression level in liver
GenevisibleiO88777 RN

Interactioni

Subunit structurei

Homodimer. Component of the gamma-secretase complex, a complex composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2. Such minimal complex is sufficient for secretase activity, although other components may exist. Interacts with DOCK3. Interacts with HERPUD1, FLNA and FLNB (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049605

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi414 – 416PAL3

Domaini

The PAL motif is required for normal active site conformation.By similarity

Sequence similaritiesi

Belongs to the peptidase A22A family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2736 Eukaryota
ENOG410XPZD LUCA
GeneTreeiENSGT00390000016593
HOGENOMiHOG000240228
HOVERGENiHBG011375
InParanoidiO88777
KOiK04522
OMAiTLCMLVV
OrthoDBiEOG091G0C72
PhylomeDBiO88777
TreeFamiTF315040

Family and domain databases

InterProiView protein in InterPro
IPR001493 Pept_A22A_PS2
IPR001108 Peptidase_A22A
IPR006639 Preselin/SPP
PANTHERiPTHR10202 PTHR10202, 2 hits
PfamiView protein in Pfam
PF01080 Presenilin, 2 hits
PRINTSiPR01072 PRESENILIN
PR01074 PRESENILIN2
SMARTiView protein in SMART
SM00730 PSN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88777-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLTFMASDSE EEVCDERTSL MSAESPTSRS CQDSRPGPED GENTAQWRSQ
60 70 80 90 100
ENEDDCEEDP DHYACSGVPG RPSGLEEELT LKYGAKHVIM LFVPVTLCMI
110 120 130 140 150
VVVATIKSVR FYTEKNGQLI YTPFTEDTPS VGQRLLNSVL NTLIMISVIV
160 170 180 190 200
VMTIFLVVLY KYRCYKFIHG WLIMSSLMLL FLFTYIYLGE VFKTYNVAMD
210 220 230 240 250
YPTLFLAVWN FGAVGMVCIH WKGPLVLQQA YLIVISALMA LVFIKYLPEW
260 270 280 290 300
SAWVILGAIS VYDLVAVLCP KGPLRMLVET AQERNEPIFP ALIYSSAMVW
310 320 330 340 350
TVGMAKLDPS SQGALQLPYD PEMEEDSYDS FGEPSYPEAF EAPQPGYPGE
360 370 380 390 400
EPEEEEERGV KLGLGDFIFY SVLVGKAAAT GNGDWSTTLA CFIAILIGLC
410 420 430 440
LTLLLLAVFK KALPALPISI TFGLIFYFST DNLVRPFMDT LASHQLYI
Length:448
Mass (Da):50,051
Last modified:July 15, 1999 - v2
Checksum:i299A7C416405046C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7S → T in CAA67663 (Ref. 1) Curated1
Sequence conflicti86 – 87KH → ND in BAA20406 (PubMed:9545577).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99267 mRNA Translation: CAA67663.1
D83700 mRNA Translation: BAA22832.1
AB004454 mRNA Translation: BAA20406.1
BC078805 mRNA Translation: AAH78805.1
RefSeqiNP_112349.2, NM_031087.2
XP_006250467.1, XM_006250405.3
XP_006250469.1, XM_006250407.3
XP_006250470.1, XM_006250408.3
XP_008768099.1, XM_008769877.2
XP_017454428.1, XM_017598939.1
UniGeneiRn.11045

Genome annotation databases

EnsembliENSRNOT00000040203; ENSRNOP00000049605; ENSRNOG00000002879
ENSRNOT00000091715; ENSRNOP00000070964; ENSRNOG00000002879
GeneIDi81751
KEGGirno:81751

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99267 mRNA Translation: CAA67663.1
D83700 mRNA Translation: BAA22832.1
AB004454 mRNA Translation: BAA20406.1
BC078805 mRNA Translation: AAH78805.1
RefSeqiNP_112349.2, NM_031087.2
XP_006250467.1, XM_006250405.3
XP_006250469.1, XM_006250407.3
XP_006250470.1, XM_006250408.3
XP_008768099.1, XM_008769877.2
XP_017454428.1, XM_017598939.1
UniGeneiRn.11045

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049605

Protein family/group databases

MEROPSiA22.002

PTM databases

iPTMnetiO88777
PhosphoSitePlusiO88777

Proteomic databases

PaxDbiO88777
PRIDEiO88777

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000040203; ENSRNOP00000049605; ENSRNOG00000002879
ENSRNOT00000091715; ENSRNOP00000070964; ENSRNOG00000002879
GeneIDi81751
KEGGirno:81751

Organism-specific databases

CTDi5664
RGDi621060 Psen2

Phylogenomic databases

eggNOGiKOG2736 Eukaryota
ENOG410XPZD LUCA
GeneTreeiENSGT00390000016593
HOGENOMiHOG000240228
HOVERGENiHBG011375
InParanoidiO88777
KOiK04522
OMAiTLCMLVV
OrthoDBiEOG091G0C72
PhylomeDBiO88777
TreeFamiTF315040

Enzyme and pathway databases

ReactomeiR-RNO-1251985 Nuclear signaling by ERBB4
R-RNO-193692 Regulated proteolysis of p75NTR
R-RNO-205043 NRIF signals cell death from the nucleus
R-RNO-3928665 EPH-ephrin mediated repulsion of cells
R-RNO-9013507 NOTCH3 Activation and Transmission of Signal to the Nucleus
R-RNO-9017802 Noncanonical activation of NOTCH3

Miscellaneous databases

PROiPR:O88777

Gene expression databases

BgeeiENSRNOG00000002879 Expressed in 10 organ(s), highest expression level in liver
GenevisibleiO88777 RN

Family and domain databases

InterProiView protein in InterPro
IPR001493 Pept_A22A_PS2
IPR001108 Peptidase_A22A
IPR006639 Preselin/SPP
PANTHERiPTHR10202 PTHR10202, 2 hits
PfamiView protein in Pfam
PF01080 Presenilin, 2 hits
PRINTSiPR01072 PRESENILIN
PR01074 PRESENILIN2
SMARTiView protein in SMART
SM00730 PSN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPSN2_RAT
AccessioniPrimary (citable) accession number: O88777
Secondary accession number(s): O08947, O35546
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 15, 1999
Last modified: November 7, 2018
This is version 145 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again