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Protein

Calcium-binding protein 1

Gene

Cabp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Modulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors (ITPRs). Inhibits agonist-induced intracellular calcium signaling (By similarity). Enhances inactivation and does not support calcium-dependent facilitation of voltage-dependent P/Q-type calcium channels (PubMed:11865310). Causes calcium-dependent facilitation and inhibits inactivation of L-type calcium channels by binding to the same sites as calmodulin in the C-terminal domain of CACNA1C, but has an opposite effect on channel function. Suppresses the calcium-dependent inactivation of CACNA1D. Inhibits TRPC5 channels. Prevents NMDA receptor-induced cellular degeneration. Required for the normal transfer of light signals through the retina (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi166MagnesiumBy similarity1
Metal bindingi168MagnesiumBy similarity1
Metal bindingi170MagnesiumBy similarity1
Metal bindingi172Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi243Calcium 1By similarity1
Metal bindingi245Calcium 1By similarity1
Metal bindingi247Calcium 1By similarity1
Metal bindingi249Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi254Calcium 1By similarity1
Metal bindingi280Calcium 2By similarity1
Metal bindingi281Calcium 2; via amide nitrogenBy similarity1
Metal bindingi282Calcium 2By similarity1
Metal bindingi284Calcium 2By similarity1
Metal bindingi285Calcium 2; via amide nitrogenBy similarity1
Metal bindingi286Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi288Calcium 2By similarity1
Metal bindingi291Calcium 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi166 – 1771PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi243 – 2542PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi280 – 2913PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • calcium ion binding Source: RGD
  • ion channel binding Source: RGD
  • nuclear localization sequence binding Source: UniProtKB
  • protein domain specific binding Source: RGD
  • protein homodimerization activity Source: RGD
  • type 3 metabotropic glutamate receptor binding Source: RGD

GO - Biological processi

  • multicellular organism development Source: UniProtKB-KW
  • negative regulation of cell communication by electrical coupling Source: RGD
  • negative regulation of protein import into nucleus Source: UniProtKB
  • negative regulation of voltage-gated calcium channel activity Source: RGD
  • regulation of postsynapse to nucleus signaling pathway Source: SynGO
  • regulation of synaptic plasticity Source: RGD
  • regulation of voltage-gated calcium channel activity Source: RGD
  • response to stimulus Source: UniProtKB-KW
  • visual perception Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein
Biological processSensory transduction, Vision
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding protein 1
Short name:
CaBP1
Alternative name(s):
Caldendrin
Gene namesi
Name:Cabp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi620385 Cabp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000735151 – 298Calcium-binding protein 1Add BLAST298
Isoform 2 (identifier: O88751-2)
Initiator methionineiRemovedBy similarity1 Publication
Isoform 3 (identifier: O88751-3)
Initiator methionineiRemovedBy similarity1 Publication

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251PhosphoserineBy similarity1
Isoform 2 (identifier: O88751-2)
Lipidationi2N-myristoyl glycineBy similarity1 Publication1
Lipidationi4S-palmitoyl cysteineBy similarity1 Publication1
Isoform 3 (identifier: O88751-3)
Lipidationi2N-myristoyl glycineBy similarity1 Publication1
Lipidationi4S-palmitoyl cysteineBy similarity1 Publication1

Post-translational modificationi

Phosphorylated. The phosphorylation regulates the activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO88751
PRIDEiO88751

Expressioni

Tissue specificityi

Somatodendritic compartment of neurons. Restricted expression in retina to a subpopulation of amacrine, bipolar, and ganglion cells. According to PubMed:11906216, expression is heterogeneous within brain regions and their major cell types and does not match with those of marker proteins for characterized neuronal subpopulations.1 Publication

Developmental stagei

Its expression is regulated differentially in retinal cell types during development.

Gene expression databases

BgeeiENSRNOG00000001173
ExpressionAtlasiO88751 baseline and differential
GenevisibleiO88751 RN

Interactioni

Subunit structurei

Interacts ITPR1, ITPR2 and ITPR3. The strength of this interaction inversely correlates with calcium concentration. Interacts with CACNA1A (via C-terminal CDB motif) in the pre- and postsynaptic membranes. Interacts with CACNA1C. Interacts with CACNA1D (By similarity). Interacts (via EF-hands 1 and 2) at microtubules with MAP1LC3B. Interacts (via EF-hands 1 and 2) with NSMF (via the central NLS-containing motif region), the interaction occurs in a calcium dependent manner after synaptic NMDA receptor stimulation and prevents nuclear import of NSMF. Interacts with MYO1C and TRPC5. Interacts with SPACA9 (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • calcium-dependent protein binding Source: UniProtKB
  • ion channel binding Source: RGD
  • protein domain specific binding Source: RGD
  • protein homodimerization activity Source: RGD
  • type 3 metabotropic glutamate receptor binding Source: RGD

Protein-protein interaction databases

CORUMiO88751
IntActiO88751, 3 interactors
STRINGi10116.ENSRNOP00000001551

Structurei

3D structure databases

ProteinModelPortaliO88751
SMRiO88751
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini153 – 188EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini189 – 224EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini230 – 265EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini267 – 298EF-hand 4PROSITE-ProRule annotationAdd BLAST32

Domaini

EF-1 binds magnesium constitutively under physiological conditions, EF-3 and EF-4 bind calcium cooperatively and EF-2 binds neither calcium nor magnesium.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0027 Eukaryota
COG5126 LUCA
GeneTreeiENSGT00760000118901
HOGENOMiHOG000233018
HOVERGENiHBG012180
InParanoidiO88751
OMAiNVLGPAC
OrthoDBiEOG091G0PB4
PhylomeDBiO88751

Family and domain databases

CDDicd00051 EFh, 1 hit
InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PfamiView protein in Pfam
PF13499 EF-hand_7, 2 hits
SMARTiView protein in SMART
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 3 hits
PS50222 EF_HAND_2, 4 hits

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O88751-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSHIAKSES KTSLLKAAAA SGGSRAPRHS SARDPGLRGR RLPGPCPDSP
60 70 80 90 100
ATCGDPSSRR PLCRPVPRDE GARGSRRGLP QAHCRPRETL PPARGRDGEE
110 120 130 140 150
RGLAPALSLR GSLRSRGRGD PAPAGTPEAD PFLHQLRPML SSAFGQDRSL
160 170 180 190 200
RPEEIEELRE AFREFDKDKD GYINCRDLGN CMRTMGYMPT EMELIELSQQ
210 220 230 240 250
INMNLGGHVD FDDFVELMGP KLLAETADMI GVKELRDAFR EFDTNGDGEI
260 270 280 290
STSELREAMR KLLGHQVGHR DIEEIIRDVD LNGDGRVDFE EFVRMMSR
Note: Major isoform expressed in the brain.
Length:298
Mass (Da):33,017
Last modified:May 27, 2002 - v2
Checksum:i8A9B74CD6B9F6875
GO
Isoform 2 (identifier: O88751-2) [UniParc]FASTAAdd to basket
Also known as: Caldendrin-S1

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLRNLSRK

Note: Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.By similarity1 Publication
Show »
Length:167
Mass (Da):19,354
Checksum:i3C65D1F2C4338AF3
GO
Isoform 3 (identifier: O88751-3) [UniParc]FASTAAdd to basket
Also known as: Caldendrin-S2

The sequence of this isoform differs from the canonical sequence as follows:
     1-146: MSSHIAKSES...RPMLSSAFGQ → MGNCVKSPLR...RKGFAENRQP

Note: Minor isoform expressed in the brain, in the granule cell layer of the cerebellum, at low level. Not developmentally regulated.By similarity1 Publication
Show »
Length:227
Mass (Da):25,903
Checksum:iC0BCC66C8FF0C88F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0261571 – 146MSSHI…SAFGQ → MGNCVKSPLRNLSRK in isoform 2. 1 PublicationAdd BLAST146
Alternative sequenceiVSP_0261581 – 146MSSHI…SAFGQ → MGNCVKSPLRNLSRKMRQEE KTSYMAVQTSEDGLADGGEL PGPLMMLAQNCAVMHNLLGP ACIFLRKGFAENRQP in isoform 3. 1 PublicationAdd BLAST146

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y17048 mRNA Translation: CAD20347.1
AJ315761 mRNA Translation: CAC43037.1
AJ315657 mRNA Translation: CAC42417.1
RefSeqiNP_001028847.1, NM_001033675.1 [O88751-2]
NP_001028848.1, NM_001033676.1
NP_598213.1, NM_133529.2 [O88751-3]
UniGeneiRn.23560

Genome annotation databases

EnsembliENSRNOT00000001552; ENSRNOP00000001552; ENSRNOG00000001173 [O88751-3]
ENSRNOT00000039281; ENSRNOP00000033685; ENSRNOG00000001173 [O88751-2]
GeneIDi171051
KEGGirno:171051
UCSCiRGD:620385 rat [O88751-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCABP1_RAT
AccessioniPrimary (citable) accession number: O88751
Secondary accession number(s): Q711K8, Q91WZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 27, 2002
Last modified: June 20, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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