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Protein

C-terminal-binding protein 1

Gene

Ctbp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.5 Publications

Cofactori

NAD+By similarityNote: Cofactor binding induces a conformational change.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei204NADBy similarity1
Active sitei266By similarity1
Binding sitei290NADBy similarity1
Active sitei295By similarity1
Active sitei315Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 185NADBy similarity6
Nucleotide bindingi237 – 243NADBy similarity7
Nucleotide bindingi264 – 266NADBy similarity3
Nucleotide bindingi315 – 318NADBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Repressor
Biological processDifferentiation, Transcription, Transcription regulation
LigandNAD

Enzyme and pathway databases

ReactomeiR-MMU-3769402 Deactivation of the beta-catenin transactivating complex
R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-4641265 Repression of WNT target genes

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein 1 (EC:1.1.1.-)
Short name:
CtBP1
Gene namesi
Name:Ctbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1201685 Ctbp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760421 – 441C-terminal-binding protein 1Add BLAST441

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei300PhosphoserineBy similarity1
Modified residuei423PhosphoserineBy similarity1
Cross-linki429Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

ADP-ribosylated; when cells are exposed to brefeldin A.By similarity
The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-423 induces proteasomal degradation (By similarity).By similarity
Sumoylation on Lys-429 is promoted by the E3 SUMO-protein ligase CBX4.By similarity

Keywords - PTMi

ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO88712
PaxDbiO88712
PeptideAtlasiO88712
PRIDEiO88712

PTM databases

iPTMnetiO88712
PhosphoSitePlusiO88712

Expressioni

Tissue specificityi

Expressed in a wide range of adult tissues.1 Publication

Developmental stagei

Expressed throughout the developmental stages.1 Publication

Gene expression databases

BgeeiENSMUSG00000037373 Expressed in 302 organ(s), highest expression level in ear
CleanExiMM_CTBP1
ExpressionAtlasiO88712 baseline and differential
GenevisibleiO88712 MM

Interactioni

Subunit structurei

Homo- or heterodimer. Heterodimer with CTBP2. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif). Interacts with PNN, MECOM and ZFHX1B. Interacts with ZNF366 (via PXDLS motif) (By similarity). Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)-specific genes expression. Interacts with GLIS2, HIPK2, FOXP1, FOXP2, HDAC4, HDAC5, HDAC9, NRIP1, WIZ and ZNF217. Interacts with BCL6; the interaction is required for BCL6 transcriptional autoinhibition and inhibition of some BCL6 target genes. Interacts with IKZF4. Interacts with MCRIP1 (unphosphorylated form, via the PXDLS motif); competitively inhibiting CTBP-ZEB1 interaction (By similarity).By similarity9 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198961, 17 interactors
CORUMiO88712
DIPiDIP-33907N
IntActiO88712, 19 interactors
MINTiO88712
STRINGi10090.ENSMUSP00000078682

Structurei

3D structure databases

ProteinModelPortaliO88712
SMRiO88712
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 70Interaction with GLIS2 1Add BLAST70
Regioni288 – 360Interaction with GLIS2 2Add BLAST73

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067 Eukaryota
COG0111 LUCA
GeneTreeiENSGT00530000063021
HOVERGENiHBG001898
InParanoidiO88712
KOiK04496
OMAiYHAIGIR
OrthoDBiEOG091G08GS
PhylomeDBiO88712
TreeFamiTF313593

Family and domain databases

InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029753 D-isomer_DH_CS
IPR029752 D-isomer_DH_CS1
IPR006140 D-isomer_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00065 D_2_HYDROXYACID_DH_1, 1 hit
PS00671 D_2_HYDROXYACID_DH_3, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 7 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O88712-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGSSHLLNKG LPLGVRPPIM NGPMHPRPLV ALLDGRDCTV EMPILKDVAT
60 70 80 90 100
VAFCDAQSTQ EIHEKVLNEA VGALMYHTIT LTREDLEKFK ALRIIVRIGS
110 120 130 140 150
GFDNIDIKSA GDLGIAVCNV PAASVEETAD STLCHILNLY RRTTWLHQAL
160 170 180 190 200
REGTRVQSVE QIREVASGAA RIRGETLGII GLGRVGQAVA LRAKAFGFNV
210 220 230 240 250
LFYDPYLSDG IERALGLQRV STLQDLLFHS DCVTLHCGLN EHNHHLINDF
260 270 280 290 300
TVKQMRQGAF LVNTARGGLV DEKALAQALK EGRIRGAALD VHESEPFSFS
310 320 330 340 350
QGPLKDAPNL ICTPHAAWYS EQASIEMREE AAREIRRAIT GRIPDSLKNC
360 370 380 390 400
VNKDHLTAAT HWASMDPAVV HPELNGAAYS RYPPGVVSVA PTGIPAAVEG
410 420 430 440
IVPSAMSLSH GLPPVAHPPH APSPGQTVKP EADRDHTSDQ L
Length:441
Mass (Da):47,745
Last modified:May 1, 2007 - v2
Checksum:i2105CC8D69D915F4
GO
Isoform 2 (identifier: O88712-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-13: MGSSHLLNKGLPL → MS

Show »
Length:430
Mass (Da):46,614
Checksum:i0A36DBF66E6A8605
GO
Isoform 3 (identifier: O88712-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-74: Missing.

Note: No experimental confirmation available.
Show »
Length:367
Mass (Da):39,848
Checksum:i0E71CBDFDBF2EDA9
GO

Computationally mapped potential isoform sequencesi

There are 7 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YU62A0A0J9YU62_MOUSE
C-terminal-binding protein 1
Ctbp1
429Annotation score:
A0A0J9YTW3A0A0J9YTW3_MOUSE
C-terminal-binding protein 1
Ctbp1
180Annotation score:
A0A0J9YUM4A0A0J9YUM4_MOUSE
C-terminal-binding protein 1
Ctbp1
337Annotation score:
A0A0J9YVI3A0A0J9YVI3_MOUSE
C-terminal-binding protein 1
Ctbp1
85Annotation score:
A0A0J9YUR5A0A0J9YUR5_MOUSE
C-terminal-binding protein 1
Ctbp1
117Annotation score:
A0A0J9YVC3A0A0J9YVC3_MOUSE
C-terminal-binding protein 1
Ctbp1
31Annotation score:
A0A0J9YU66A0A0J9YU66_MOUSE
C-terminal binding protein 1, isofo...
Ctbp1 mCG_2534
77Annotation score:

Sequence cautioni

The sequence BAE41586 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55D → G in BAE41586 (PubMed:16141072).Curated1
Sequence conflicti55D → G in BAE42587 (PubMed:16141072).Curated1
Sequence conflicti108K → R in BAE35946 (PubMed:16141072).Curated1
Sequence conflicti380Missing in CAA09219 (PubMed:10369679).Curated1
Sequence conflicti380Missing in BAE35946 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0247371 – 74Missing in isoform 3. 1 PublicationAdd BLAST74
Alternative sequenceiVSP_0247381 – 13MGSSH…KGLPL → MS in isoform 2. 2 PublicationsAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010483 mRNA Translation: CAA09219.1
AB033122 mRNA Translation: BAA85180.1
AK133816 mRNA Translation: BAE21859.1
AK160658 mRNA Translation: BAE35946.1
AK165276 mRNA Translation: BAE38115.1
AK170133 mRNA Translation: BAE41586.1 Different initiation.
AK171650 mRNA Translation: BAE42587.1
BC013702 mRNA Translation: AAH13702.1
BC015071 mRNA Translation: AAH15071.1
BC042425 mRNA Translation: AAH42425.1
CCDSiCCDS19201.1 [O88712-1]
CCDS80255.1 [O88712-3]
RefSeqiNP_001185788.1, NM_001198859.1
NP_001185789.1, NM_001198860.1
NP_001185790.1, NM_001198861.1 [O88712-2]
NP_001297464.1, NM_001310535.1 [O88712-3]
NP_038530.1, NM_013502.3 [O88712-1]
UniGeneiMm.240076
Mm.7286

Genome annotation databases

EnsembliENSMUST00000079746; ENSMUSP00000078682; ENSMUSG00000037373 [O88712-1]
ENSMUST00000201575; ENSMUSP00000144554; ENSMUSG00000037373 [O88712-3]
GeneIDi13016
KEGGimmu:13016
UCSCiuc008xaj.2 mouse [O88712-1]
uc008xak.2 mouse [O88712-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010483 mRNA Translation: CAA09219.1
AB033122 mRNA Translation: BAA85180.1
AK133816 mRNA Translation: BAE21859.1
AK160658 mRNA Translation: BAE35946.1
AK165276 mRNA Translation: BAE38115.1
AK170133 mRNA Translation: BAE41586.1 Different initiation.
AK171650 mRNA Translation: BAE42587.1
BC013702 mRNA Translation: AAH13702.1
BC015071 mRNA Translation: AAH15071.1
BC042425 mRNA Translation: AAH42425.1
CCDSiCCDS19201.1 [O88712-1]
CCDS80255.1 [O88712-3]
RefSeqiNP_001185788.1, NM_001198859.1
NP_001185789.1, NM_001198860.1
NP_001185790.1, NM_001198861.1 [O88712-2]
NP_001297464.1, NM_001310535.1 [O88712-3]
NP_038530.1, NM_013502.3 [O88712-1]
UniGeneiMm.240076
Mm.7286

3D structure databases

ProteinModelPortaliO88712
SMRiO88712
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198961, 17 interactors
CORUMiO88712
DIPiDIP-33907N
IntActiO88712, 19 interactors
MINTiO88712
STRINGi10090.ENSMUSP00000078682

PTM databases

iPTMnetiO88712
PhosphoSitePlusiO88712

Proteomic databases

MaxQBiO88712
PaxDbiO88712
PeptideAtlasiO88712
PRIDEiO88712

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079746; ENSMUSP00000078682; ENSMUSG00000037373 [O88712-1]
ENSMUST00000201575; ENSMUSP00000144554; ENSMUSG00000037373 [O88712-3]
GeneIDi13016
KEGGimmu:13016
UCSCiuc008xaj.2 mouse [O88712-1]
uc008xak.2 mouse [O88712-2]

Organism-specific databases

CTDi1487
MGIiMGI:1201685 Ctbp1

Phylogenomic databases

eggNOGiKOG0067 Eukaryota
COG0111 LUCA
GeneTreeiENSGT00530000063021
HOVERGENiHBG001898
InParanoidiO88712
KOiK04496
OMAiYHAIGIR
OrthoDBiEOG091G08GS
PhylomeDBiO88712
TreeFamiTF313593

Enzyme and pathway databases

ReactomeiR-MMU-3769402 Deactivation of the beta-catenin transactivating complex
R-MMU-3899300 SUMOylation of transcription cofactors
R-MMU-4641265 Repression of WNT target genes

Miscellaneous databases

ChiTaRSiCtbp1 mouse
PROiPR:O88712
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037373 Expressed in 302 organ(s), highest expression level in ear
CleanExiMM_CTBP1
ExpressionAtlasiO88712 baseline and differential
GenevisibleiO88712 MM

Family and domain databases

InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029753 D-isomer_DH_CS
IPR029752 D-isomer_DH_CS1
IPR006140 D-isomer_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00065 D_2_HYDROXYACID_DH_1, 1 hit
PS00671 D_2_HYDROXYACID_DH_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCTBP1_MOUSE
AccessioniPrimary (citable) accession number: O88712
Secondary accession number(s): Q3TAT1
, Q3TDL5, Q3TUM5, Q91WI6, Q91YX3, Q9QYG2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 2007
Last modified: November 7, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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