UniProtKB - O88708 (ORC4_MOUSE)
Protein
Origin recognition complex subunit 4
Gene
Orc4
Organism
Mus musculus (Mouse)
Status
Functioni
Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity). Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 65 – 72 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- DNA replication origin binding Source: MGI
- nucleotide binding Source: MGI
GO - Biological processi
- DNA replication Source: MGI
- DNA replication initiation Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68616, Assembly of the ORC complex at the origin of replication R-MMU-68689, CDC6 association with the ORC:origin complex R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex |
Names & Taxonomyi
Protein namesi | Recommended name: Origin recognition complex subunit 4 |
Gene namesi | Name:Orc4 Synonyms:Orc4l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1347043, Orc4 |
Subcellular locationi
Nucleus
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear chromosome, telomeric region Source: Ensembl
- nuclear origin of replication recognition complex Source: UniProtKB
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
Other locations
- origin recognition complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127088 | 1 – 433 | Origin recognition complex subunit 4Add BLAST | 433 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 7 | N6-methyllysineBy similarity | 1 |
Keywords - PTMi
MethylationProteomic databases
EPDi | O88708 |
MaxQBi | O88708 |
PaxDbi | O88708 |
PeptideAtlasi | O88708 |
PRIDEi | O88708 |
PTM databases
iPTMneti | O88708 |
PhosphoSitePlusi | O88708 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000026761, Expressed in otic placode and 296 other tissues |
ExpressionAtlasi | O88708, baseline and differential |
Genevisiblei | O88708, MM |
Interactioni
Subunit structurei
Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase (By similarity).
Interacts with DBF4 (PubMed:12614612).
Interacts with POLQ (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 204980, 1 interactor |
ComplexPortali | CPX-1915, Nuclear origin of replication recognition complex |
CORUMi | O88708 |
STRINGi | 10090.ENSMUSP00000028098 |
Miscellaneous databases
RNActi | O88708, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ORC4 family.Curated
Phylogenomic databases
eggNOGi | KOG2228, Eukaryota |
GeneTreei | ENSGT00390000016542 |
HOGENOMi | CLU_007115_0_1_1 |
InParanoidi | O88708 |
OMAi | KEWAING |
OrthoDBi | 1543398at2759 |
TreeFami | TF101094 |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041664, AAA_16 IPR016527, ORC4 IPR032705, ORC4_C IPR027417, P-loop_NTPase |
PANTHERi | PTHR12087, PTHR12087, 1 hit |
Pfami | View protein in Pfam PF13191, AAA_16, 1 hit PF14629, ORC4_C, 1 hit |
PIRSFi | PIRSF007858, ORC4, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
O88708-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSRKTKSNA HAECLSQVQR ILRERFCHHS PHSNLFGVQV QYKHLIELLK
60 70 80 90 100
RTAIYGESNS VLIVGPRGSG KTTLLNHALK ELMEIEVSEN VIQVHLNGLL
110 120 130 140 150
QTNEKIALKE ITRQLNLDNV VEDKVFGSFA ENLSFLLEAL QKGDRTSSCP
160 170 180 190 200
VIFILDEFDI FAHQKNQTLL YNLFDISQSA QTPVAVIGLT CRLDILELLE
210 220 230 240 250
KRVKSRFSHR QIHLMNSFDF PQYLKIFKEQ LSLPAEFPDK AFAERWNENV
260 270 280 290 300
HCLSEDSTVL EVLQKHFSVN KNLQSLHMLL MLALNRVTVS HPFMTSADLM
310 320 330 340 350
EAQHMCSLDS KANIVHGLSV LEICLIIAMK HLNDIYEEEP FNFQMVYNEF
360 370 380 390 400
QKFIQRKAHS VYNFEKPVVM KAFEHLQQLE LIKPVERTSV NSQREYQLVK
410 420 430
LLLDNTQIMN ALQKYSNCPT DVRQWATSSL SWL
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA2AI40 | A2AI40_MOUSE | Origin recognition complex subunit ... | Orc4 | 237 | Annotation score: | ||
Q9CZF7 | Q9CZF7_MOUSE | Origin recognition complex subunit ... | Orc4 Orc4l | 138 | Annotation score: | ||
A2AI43 | A2AI43_MOUSE | Origin recognition complex subunit ... | Orc4 | 72 | Annotation score: | ||
A2AI42 | A2AI42_MOUSE | Origin recognition complex subunit ... | Orc4 | 49 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 141 | Q → K in CAA76188 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y16386 mRNA Translation: CAA76188.1 AJ003140 mRNA Translation: CAA05896.1 AK010258 mRNA Translation: BAB26801.1 AK147823 mRNA Translation: BAE28161.1 AK169291 mRNA Translation: BAE41048.1 AL732317 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL26871.1 BC015072 mRNA Translation: AAH15072.1 BC019748 mRNA Translation: AAH19748.1 |
CCDSi | CCDS16022.1 |
RefSeqi | NP_036088.3, NM_011958.3 XP_006498126.1, XM_006498063.3 XP_006498127.1, XM_006498064.3 XP_006498128.1, XM_006498065.3 |
Genome annotation databases
Ensembli | ENSMUST00000028098; ENSMUSP00000028098; ENSMUSG00000026761 |
GeneIDi | 26428 |
KEGGi | mmu:26428 |
UCSCi | uc008jpp.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y16386 mRNA Translation: CAA76188.1 AJ003140 mRNA Translation: CAA05896.1 AK010258 mRNA Translation: BAB26801.1 AK147823 mRNA Translation: BAE28161.1 AK169291 mRNA Translation: BAE41048.1 AL732317 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL26871.1 BC015072 mRNA Translation: AAH15072.1 BC019748 mRNA Translation: AAH19748.1 |
CCDSi | CCDS16022.1 |
RefSeqi | NP_036088.3, NM_011958.3 XP_006498126.1, XM_006498063.3 XP_006498127.1, XM_006498064.3 XP_006498128.1, XM_006498065.3 |
3D structure databases
SMRi | O88708 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204980, 1 interactor |
ComplexPortali | CPX-1915, Nuclear origin of replication recognition complex |
CORUMi | O88708 |
STRINGi | 10090.ENSMUSP00000028098 |
PTM databases
iPTMneti | O88708 |
PhosphoSitePlusi | O88708 |
Proteomic databases
EPDi | O88708 |
MaxQBi | O88708 |
PaxDbi | O88708 |
PeptideAtlasi | O88708 |
PRIDEi | O88708 |
Protocols and materials databases
Antibodypediai | 4189, 231 antibodies |
DNASUi | 26428 |
Genome annotation databases
Ensembli | ENSMUST00000028098; ENSMUSP00000028098; ENSMUSG00000026761 |
GeneIDi | 26428 |
KEGGi | mmu:26428 |
UCSCi | uc008jpp.2, mouse |
Organism-specific databases
CTDi | 5000 |
MGIi | MGI:1347043, Orc4 |
Phylogenomic databases
eggNOGi | KOG2228, Eukaryota |
GeneTreei | ENSGT00390000016542 |
HOGENOMi | CLU_007115_0_1_1 |
InParanoidi | O88708 |
OMAi | KEWAING |
OrthoDBi | 1543398at2759 |
TreeFami | TF101094 |
Enzyme and pathway databases
Reactomei | R-MMU-176187, Activation of ATR in response to replication stress R-MMU-68616, Assembly of the ORC complex at the origin of replication R-MMU-68689, CDC6 association with the ORC:origin complex R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68867, Assembly of the pre-replicative complex R-MMU-68949, Orc1 removal from chromatin R-MMU-68962, Activation of the pre-replicative complex |
Miscellaneous databases
BioGRID-ORCSi | 26428, 9 hits in 18 CRISPR screens |
ChiTaRSi | Orc4, mouse |
PROi | PR:O88708 |
RNActi | O88708, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000026761, Expressed in otic placode and 296 other tissues |
ExpressionAtlasi | O88708, baseline and differential |
Genevisiblei | O88708, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR041664, AAA_16 IPR016527, ORC4 IPR032705, ORC4_C IPR027417, P-loop_NTPase |
PANTHERi | PTHR12087, PTHR12087, 1 hit |
Pfami | View protein in Pfam PF13191, AAA_16, 1 hit PF14629, ORC4_C, 1 hit |
PIRSFi | PIRSF007858, ORC4, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ORC4_MOUSE | |
Accessioni | O88708Primary (citable) accession number: O88708 Secondary accession number(s): Q91V62, Q9QYX1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families