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Protein

Serine/threonine-protein kinase 16

Gene

Stk16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Membrane-associated protein kinase that phosphorylates on serine and threonine residues. In vitro substrates include DRG1, ENO1 and EIF4EBP1. Also autophosphorylates (By similarity). May be involved in secretory vesicle trafficking or intracellular signaling. May have a role in regulating stromal-epithelial interactions that occur during ductal morphogenesis in the mammary gland. May be involved in TGF-beta signaling. Able to autophosphorylate on Tyr residue; it is however unclear whether it has tyrosine-protein kinase toward other proteins.By similarity1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei148Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase 16 (EC:2.7.11.1)
Alternative name(s):
Embryo-derived protein kinase
Short name:
Edpk
Myristoylated and palmitoylated serine/threonine-protein kinase
Short name:
MPSK
Protein kinase Krct
Protein kinase PKL12
TGF-beta-stimulated factor 1
Short name:
TSF-1
Tyrosine-protein kinase STK16 (EC:2.7.10.2)
Gene namesi
Name:Stk16
Synonyms:Edpk, Krct, Mpsk1, Pkl12, Tsf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1313271 Stk16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000867022 – 305Serine/threonine-protein kinase 16Add BLAST304

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1
Lipidationi6S-palmitoyl cysteineBy similarity1
Lipidationi8S-palmitoyl cysteineBy similarity1
Modified residuei197Phosphoserine; by autocatalysisBy similarity1
Modified residuei198Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Mainly autophosphorylated on serine/threonine residues. Also autophosphorylated on Tyr-198 (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

EPDiO88697
MaxQBiO88697
PaxDbiO88697
PeptideAtlasiO88697
PRIDEiO88697

PTM databases

PhosphoSitePlusiO88697
SwissPalmiO88697

Expressioni

Tissue specificityi

Ubiquitously expressed at low levels. Relatively higher levels in testis, kidney and liver.2 Publications

Developmental stagei

Expressed at all stages of developing embryo.1 Publication

Gene expression databases

BgeeiENSMUSG00000026201 Expressed in 289 organ(s), highest expression level in colon
CleanExiMM_STK16
ExpressionAtlasiO88697 baseline and differential
GenevisibleiO88697 MM

Interactioni

Subunit structurei

Monomer. Interacts with DRG1 (via its N-terminal); the interaction phosphorylates DRG1.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027401

Structurei

3D structure databases

ProteinModelPortaliO88697
SMRiO88697
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST274

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni166 – 202Activation loopBy similarityAdd BLAST37

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2345 Eukaryota
ENOG410YN06 LUCA
GeneTreeiENSGT00550000075037
HOGENOMiHOG000157694
HOVERGENiHBG054359
InParanoidiO88697
KOiK08856
OMAiSFVYLAQ
OrthoDBiEOG091G0F0S
PhylomeDBiO88697
TreeFamiTF350433

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O88697-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGHALCVCSR GTVIIDNKRY LFVQKLGEGG FSYVDLVEGL HDGHFYALKR
60 70 80 90 100
ILCHEQQDQE EAQREAEMHR LFQHPNILRL MAYSLKERGA KHEAWLLLPF
110 120 130 140 150
FKKGTLWNEI ERLKDQGSFL TEDQILPLLL GISRGLEAIH AKGYAHRDLK
160 170 180 190 200
PTNILLGDEG QPVLMDLGSM NQACIQVEGS RQALALQDWA AQRCTISYRA
210 220 230 240 250
PELFSVQSHC VIDERTDVWS LGCVLYAMMF GEGPYDMVFQ KGDSVALAVQ
260 270 280 290 300
NELSIPQSPR HSSALRQLLS SMMTVDPQQR PHIPVLLSQL EALQPPAPGQ

HTTQI
Length:305
Mass (Da):34,382
Last modified:January 23, 2007 - v3
Checksum:iEB4725D26B210537
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WQT0A0A087WQT0_MOUSE
Serine/threonine-protein kinase 16
Stk16
219Annotation score:
Q05DP1Q05DP1_MOUSE
Serine/threonine-protein kinase 16
Stk16
227Annotation score:
A0A087WQ12A0A087WQ12_MOUSE
Serine/threonine-protein kinase 16
Stk16
187Annotation score:
D3YXM1D3YXM1_MOUSE
Serine/threonine-protein kinase 16
Stk16
132Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti25 – 28KLGE → EIGG in BAA89662 (PubMed:10947953).Curated4
Sequence conflicti25 – 28KLGE → EIGG in BAA89499 (PubMed:16141072).Curated4
Sequence conflicti195T → A in BAA89499 (PubMed:16141072).Curated1
Sequence conflicti267 – 268QL → HV in AAD02811 (PubMed:10364453).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005790 mRNA Translation: CAA06699.1
AB017197 mRNA Translation: BAA74457.1
AF089869 mRNA Translation: AAC98502.1
AF062076 mRNA Translation: AAD02811.1
AB022285 Genomic DNA Translation: BAA89662.1
AB022179 mRNA Translation: BAA89499.1
AK149539 mRNA Translation: BAE28946.1
CH466548 Genomic DNA Translation: EDL00383.1
CH466548 Genomic DNA Translation: EDL00384.1
CH466548 Genomic DNA Translation: EDL00386.1
BC028999 mRNA Translation: AAH28999.1
CCDSiCCDS15067.1
RefSeqiNP_001264921.1, NM_001277992.1
NP_035624.3, NM_011494.5
XP_006496524.1, XM_006496461.1
UniGeneiMm.1155
Mm.17461

Genome annotation databases

EnsembliENSMUST00000027401; ENSMUSP00000027401; ENSMUSG00000026201
GeneIDi20872
KEGGimmu:20872
UCSCiuc007boh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005790 mRNA Translation: CAA06699.1
AB017197 mRNA Translation: BAA74457.1
AF089869 mRNA Translation: AAC98502.1
AF062076 mRNA Translation: AAD02811.1
AB022285 Genomic DNA Translation: BAA89662.1
AB022179 mRNA Translation: BAA89499.1
AK149539 mRNA Translation: BAE28946.1
CH466548 Genomic DNA Translation: EDL00383.1
CH466548 Genomic DNA Translation: EDL00384.1
CH466548 Genomic DNA Translation: EDL00386.1
BC028999 mRNA Translation: AAH28999.1
CCDSiCCDS15067.1
RefSeqiNP_001264921.1, NM_001277992.1
NP_035624.3, NM_011494.5
XP_006496524.1, XM_006496461.1
UniGeneiMm.1155
Mm.17461

3D structure databases

ProteinModelPortaliO88697
SMRiO88697
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000027401

PTM databases

PhosphoSitePlusiO88697
SwissPalmiO88697

Proteomic databases

EPDiO88697
MaxQBiO88697
PaxDbiO88697
PeptideAtlasiO88697
PRIDEiO88697

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027401; ENSMUSP00000027401; ENSMUSG00000026201
GeneIDi20872
KEGGimmu:20872
UCSCiuc007boh.2 mouse

Organism-specific databases

CTDi8576
MGIiMGI:1313271 Stk16

Phylogenomic databases

eggNOGiKOG2345 Eukaryota
ENOG410YN06 LUCA
GeneTreeiENSGT00550000075037
HOGENOMiHOG000157694
HOVERGENiHBG054359
InParanoidiO88697
KOiK08856
OMAiSFVYLAQ
OrthoDBiEOG091G0F0S
PhylomeDBiO88697
TreeFamiTF350433

Enzyme and pathway databases

BRENDAi2.7.11.1 3474

Miscellaneous databases

PROiPR:O88697
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026201 Expressed in 289 organ(s), highest expression level in colon
CleanExiMM_STK16
ExpressionAtlasiO88697 baseline and differential
GenevisibleiO88697 MM

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSTK16_MOUSE
AccessioniPrimary (citable) accession number: O88697
Secondary accession number(s): Q3UEG5
, Q9JMJ0, Q9JMJ1, Q9QX00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 155 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
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Main funding by: National Institutes of Health

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