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Protein

ATP-dependent Clp protease proteolytic subunit, mitochondrial

Gene

Clpp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease component of the Clp complex that cleaves peptides and various proteins in an ATP-dependent process. Has low peptidase activity in the absence of CLPX. The Clp complex can degrade CSN1S1, CSN2 and CSN3, as well as synthetic peptides (in vitro) and may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec, and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei149NucleophileBy similarity1
Active sitei174By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.92 3474

Protein family/group databases

MEROPSiS14.003

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit, mitochondrial (EC:3.4.21.92)
Alternative name(s):
Endopeptidase Clp
Gene namesi
Name:Clpp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1858213 Clpp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Homozygous pups are born at about 60 % of the expected Mendelian rate, indicating decreased intrauterine survival. Mutant mice are smaller in size than wild-type littermates, show decreased motor activity, are completely deaf after 12 months and their lifespan is decreased relative to that of wild-type littermates. Both female and male mutant mice are completely infertile due to defects in germ cell development.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 52MitochondrionBy similarityAdd BLAST52
ChainiPRO_000000551753 – 272ATP-dependent Clp protease proteolytic subunit, mitochondrialAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei196N6-succinyllysineCombined sources1
Modified residuei207N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO88696
MaxQBiO88696
PaxDbiO88696
PeptideAtlasiO88696
PRIDEiO88696

2D gel databases

REPRODUCTION-2DPAGEiIPI00133270
O88696

PTM databases

iPTMnetiO88696
PhosphoSitePlusiO88696

Expressioni

Tissue specificityi

Detected in liver (at protein level). High levels found in heart, liver and skeletal muscle.2 Publications

Gene expression databases

BgeeiENSMUSG00000002660 Expressed in 293 organ(s), highest expression level in embryo
GenevisibleiO88696 MM

Interactioni

Subunit structurei

Fourteen CLPP subunits assemble into 2 heptameric rings which stack back to back to give a disk-like structure with a central cavity. Component of the Clp complex formed by the assembly of two CLPP heptameric rings with two CLPX hexameric rings, giving rise to a symmetrical structure with two central CLPP rings flanked by a CLPX ring at either end of the complex.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207517, 1 interactor
CORUMiO88696
IntActiO88696, 3 interactors
MINTiO88696
STRINGi10090.ENSMUSP00000002735

Structurei

3D structure databases

ProteinModelPortaliO88696
SMRiO88696
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0840 Eukaryota
COG0740 LUCA
GeneTreeiENSGT00390000005830
HOGENOMiHOG000285833
HOVERGENiHBG001689
InParanoidiO88696
KOiK01358
OMAiKPPIATW
OrthoDBiEOG091G0KCO
PhylomeDBiO88696
TreeFamiTF105002

Family and domain databases

CDDicd07017 S14_ClpP_2, 1 hit
HAMAPiMF_00444 ClpP, 1 hit
InterProiView protein in InterPro
IPR001907 ClpP
IPR029045 ClpP/crotonase-like_dom_sf
IPR023562 ClpP/TepA
IPR033135 ClpP_His_AS
IPR018215 ClpP_Ser_AS
PANTHERiPTHR10381 PTHR10381, 1 hit
PfamiView protein in Pfam
PF00574 CLP_protease, 1 hit
PRINTSiPR00127 CLPPROTEASEP
SUPFAMiSSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00382 CLP_PROTEASE_HIS, 1 hit
PS00381 CLP_PROTEASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88696-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MWPRVLLGEA RVAVDGCRAL LSRLAVHFSP PWTAVSCSPL RRSLHGTATR
60 70 80 90 100
AFPLIPIVVE QTGRGERAYD IYSRLLRERI VCVMGPIDDS VASLVIAQLL
110 120 130 140 150
FLQSESNKKP IHMYINSPGG VVTAGLAIYD TMQYILNPIC TWCVGQAASM
160 170 180 190 200
GSLLLAAGSP GMRHSLPNSR IMIHQPSGGA RGQATDIAIQ AEEIMKLKKQ
210 220 230 240 250
LYNIYAKHTK QSLQVIESAM ERDRYMSPME AQEFGILDKV LVHPPQDGED
260 270
EPELVQKETA TAPTDPPAPT ST
Length:272
Mass (Da):29,800
Last modified:November 1, 1998 - v1
Checksum:i1F34C21E9C5C04D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005253 mRNA Translation: CAA06443.1
AJ012249
, AJ012250, AJ012251, AJ012252, AJ012253 Genomic DNA Translation: CAA09966.1
AK004024 mRNA Translation: BAB23132.1
AK145765 mRNA Translation: BAE26636.1
AK168053 mRNA Translation: BAE40033.1
BC001998 mRNA Translation: AAH01998.1
CCDSiCCDS28920.1
RefSeqiNP_059089.1, NM_017393.2
UniGeneiMm.287892

Genome annotation databases

EnsembliENSMUST00000002735; ENSMUSP00000002735; ENSMUSG00000002660
GeneIDi53895
KEGGimmu:53895
UCSCiuc008ddm.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005253 mRNA Translation: CAA06443.1
AJ012249
, AJ012250, AJ012251, AJ012252, AJ012253 Genomic DNA Translation: CAA09966.1
AK004024 mRNA Translation: BAB23132.1
AK145765 mRNA Translation: BAE26636.1
AK168053 mRNA Translation: BAE40033.1
BC001998 mRNA Translation: AAH01998.1
CCDSiCCDS28920.1
RefSeqiNP_059089.1, NM_017393.2
UniGeneiMm.287892

3D structure databases

ProteinModelPortaliO88696
SMRiO88696
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207517, 1 interactor
CORUMiO88696
IntActiO88696, 3 interactors
MINTiO88696
STRINGi10090.ENSMUSP00000002735

Protein family/group databases

MEROPSiS14.003

PTM databases

iPTMnetiO88696
PhosphoSitePlusiO88696

2D gel databases

REPRODUCTION-2DPAGEiIPI00133270
O88696

Proteomic databases

EPDiO88696
MaxQBiO88696
PaxDbiO88696
PeptideAtlasiO88696
PRIDEiO88696

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002735; ENSMUSP00000002735; ENSMUSG00000002660
GeneIDi53895
KEGGimmu:53895
UCSCiuc008ddm.2 mouse

Organism-specific databases

CTDi8192
MGIiMGI:1858213 Clpp

Phylogenomic databases

eggNOGiKOG0840 Eukaryota
COG0740 LUCA
GeneTreeiENSGT00390000005830
HOGENOMiHOG000285833
HOVERGENiHBG001689
InParanoidiO88696
KOiK01358
OMAiKPPIATW
OrthoDBiEOG091G0KCO
PhylomeDBiO88696
TreeFamiTF105002

Enzyme and pathway databases

BRENDAi3.4.21.92 3474

Miscellaneous databases

PROiPR:O88696
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002660 Expressed in 293 organ(s), highest expression level in embryo
GenevisibleiO88696 MM

Family and domain databases

CDDicd07017 S14_ClpP_2, 1 hit
HAMAPiMF_00444 ClpP, 1 hit
InterProiView protein in InterPro
IPR001907 ClpP
IPR029045 ClpP/crotonase-like_dom_sf
IPR023562 ClpP/TepA
IPR033135 ClpP_His_AS
IPR018215 ClpP_Ser_AS
PANTHERiPTHR10381 PTHR10381, 1 hit
PfamiView protein in Pfam
PF00574 CLP_protease, 1 hit
PRINTSiPR00127 CLPPROTEASEP
SUPFAMiSSF52096 SSF52096, 1 hit
PROSITEiView protein in PROSITE
PS00382 CLP_PROTEASE_HIS, 1 hit
PS00381 CLP_PROTEASE_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCLPP_MOUSE
AccessioniPrimary (citable) accession number: O88696
Secondary accession number(s): Q3TI13
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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