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Protein

Ceramide glucosyltransferase

Gene

Ugcg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first glycosylation step in glycosphingolipid biosynthesis, the transfer of glucose to ceramide. May also serve as a "flippase" (By similarity).By similarity

Catalytic activityi

UDP-glucose + N-acylsphingosine = UDP + D-glucosyl-N-acylsphingosine.By similarity

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei193May play an important role in binding to the inhibitors DEPC and PDMPBy similarity1
Active sitei236Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glucosylceramide biosynthetic process Source: GO_Central

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Sphingolipid metabolism

Enzyme and pathway databases

BRENDAi2.4.1.80 3474
ReactomeiR-MMU-1660662 Glycosphingolipid metabolism
UniPathwayi
UPA00222

Protein family/group databases

CAZyiGT21 Glycosyltransferase Family 21

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide glucosyltransferase (EC:2.4.1.80)
Alternative name(s):
GLCT-1
Glucosylceramide synthase
Short name:
GCS
UDP-glucose ceramide glucosyltransferase
UDP-glucose:N-acylsphingosine D-glucosyltransferase
Gene namesi
Name:Ugcg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1332243 Ugcg

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 10LumenalSequence analysis10
Transmembranei11 – 32HelicalSequence analysisAdd BLAST22
Topological domaini33 – 195CytoplasmicSequence analysisAdd BLAST163
Transmembranei196 – 215HelicalSequence analysisAdd BLAST20
Topological domaini216 – 287LumenalSequence analysisAdd BLAST72
Transmembranei288 – 304HelicalSequence analysisAdd BLAST17
Topological domaini305 – 309CytoplasmicSequence analysis5
Transmembranei310 – 328HelicalSequence analysisAdd BLAST19
Topological domaini329 – 348LumenalSequence analysisAdd BLAST20
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Topological domaini370 – 394CytoplasmicSequence analysisAdd BLAST25

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL6013

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000591771 – 394Ceramide glucosyltransferaseAdd BLAST394

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO88693
MaxQBiO88693
PaxDbiO88693
PeptideAtlasiO88693
PRIDEiO88693

PTM databases

iPTMnetiO88693
PhosphoSitePlusiO88693
SwissPalmiO88693

Expressioni

Gene expression databases

BgeeiENSMUSG00000028381 Expressed in 289 organ(s), highest expression level in morula
CleanExiMM_UGCG
GenevisibleiO88693 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030074

Chemistry databases

BindingDBiO88693

Structurei

3D structure databases

ProteinModelPortaliO88693
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi92D1Curated1
Motifi144D2Curated1
Motifi236D3Curated1
Motifi272 – 276(Q/R)XXRWCurated5

Domaini

The D1, D2, D3, (Q/R)XXRW motif is a critical part of the GCS active site, involved in catalysis and UDP-sugar binding.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2547 Eukaryota
COG1215 LUCA
GeneTreeiENSGT00390000012898
HOGENOMiHOG000039663
HOVERGENiHBG003997
InParanoidiO88693
KOiK00720
OMAiLETFFTM
OrthoDBiEOG091G0G3M
PhylomeDBiO88693
TreeFamiTF314564

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR025993 Ceramide_glucosylTrfase
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR12726 PTHR12726, 1 hit
PfamiView protein in Pfam
PF13506 Glyco_transf_21, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit

Sequencei

Sequence statusi: Complete.

O88693-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALLDLAQEG MALFGFVLFV VLWLMHFMSI IYTRLHLNKK ATDKQPYSKL
60 70 80 90 100
PGVSLLKPLK GVDPNLINNL ETFFELDYPK YEVLLCVQDH DDPAIDVCKK
110 120 130 140 150
LLGKYPNVDA RLFIGGKKVG INPKINNLMP AYEVAKYDLI WICDSGIRVI
160 170 180 190 200
PDTLTDMVNQ MTEKVGLVHG LPYVADRQGF AATLEQVYFG TSHPRSYISA
210 220 230 240 250
NVTGFKCVTG MSCLMRKDVL DQAGGLIAFA QYIAEDYFMA KAIADRGWRF
260 270 280 290 300
SMSTQVAMQN SGSYSISQFQ SRMIRWTKLR INMLPATIIC EPISECFVAS
310 320 330 340 350
LIIGWAAHHV FRWDIMVFFM CHCLAWFIFD YIQLRGVQGG TLCFSKLDYA
360 370 380 390
VAWFIRESMT IYIFLSALWD PTISWRTGRY RLRCGGTAEE ILDV
Length:394
Mass (Da):44,839
Last modified:November 1, 1998 - v1
Checksum:i8B18D09437CACE0E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012807 Genomic DNA Translation: BAA33558.1
D89866 mRNA Translation: BAA28782.1
AL808112 Genomic DNA Translation: CAM26180.1
CH466565 Genomic DNA Translation: EDL02214.1
BC050828 mRNA Translation: AAH50828.1
CCDSiCCDS18219.1
RefSeqiNP_035803.1, NM_011673.3
UniGeneiMm.198803

Genome annotation databases

EnsembliENSMUST00000030074; ENSMUSP00000030074; ENSMUSG00000028381
GeneIDi22234
KEGGimmu:22234
UCSCiuc008szr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012807 Genomic DNA Translation: BAA33558.1
D89866 mRNA Translation: BAA28782.1
AL808112 Genomic DNA Translation: CAM26180.1
CH466565 Genomic DNA Translation: EDL02214.1
BC050828 mRNA Translation: AAH50828.1
CCDSiCCDS18219.1
RefSeqiNP_035803.1, NM_011673.3
UniGeneiMm.198803

3D structure databases

ProteinModelPortaliO88693
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030074

Chemistry databases

BindingDBiO88693
ChEMBLiCHEMBL6013

Protein family/group databases

CAZyiGT21 Glycosyltransferase Family 21

PTM databases

iPTMnetiO88693
PhosphoSitePlusiO88693
SwissPalmiO88693

Proteomic databases

EPDiO88693
MaxQBiO88693
PaxDbiO88693
PeptideAtlasiO88693
PRIDEiO88693

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030074; ENSMUSP00000030074; ENSMUSG00000028381
GeneIDi22234
KEGGimmu:22234
UCSCiuc008szr.1 mouse

Organism-specific databases

CTDi7357
MGIiMGI:1332243 Ugcg

Phylogenomic databases

eggNOGiKOG2547 Eukaryota
COG1215 LUCA
GeneTreeiENSGT00390000012898
HOGENOMiHOG000039663
HOVERGENiHBG003997
InParanoidiO88693
KOiK00720
OMAiLETFFTM
OrthoDBiEOG091G0G3M
PhylomeDBiO88693
TreeFamiTF314564

Enzyme and pathway databases

UniPathwayi
UPA00222

BRENDAi2.4.1.80 3474
ReactomeiR-MMU-1660662 Glycosphingolipid metabolism

Miscellaneous databases

ChiTaRSiUgcg mouse
PROiPR:O88693
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028381 Expressed in 289 organ(s), highest expression level in morula
CleanExiMM_UGCG
GenevisibleiO88693 MM

Family and domain databases

Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR025993 Ceramide_glucosylTrfase
IPR029044 Nucleotide-diphossugar_trans
PANTHERiPTHR12726 PTHR12726, 1 hit
PfamiView protein in Pfam
PF13506 Glyco_transf_21, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCEGT_MOUSE
AccessioniPrimary (citable) accession number: O88693
Secondary accession number(s): A2AN90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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