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Protein

Diacylglycerol kinase alpha

Gene

Dgka

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Activity regulationi

Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi124 – 1351CuratedAdd BLAST12
Calcium bindingi169 – 1802CuratedAdd BLAST12
Zinc fingeri206 – 254Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri270 – 320Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 3474
ReactomeiR-MMU-114508 Effects of PIP2 hydrolysis

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase alpha (EC:2.7.1.107)
Short name:
DAG kinase alpha
Alternative name(s):
80 kDa diacylglycerol kinase
Diglyceride kinase alpha
Short name:
DGK-alpha
Gene namesi
Name:Dgka
Synonyms:Dagk1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:102952 Dgka

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184541 – 730Diacylglycerol kinase alphaAdd BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei479N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO88673
MaxQBiO88673
PaxDbiO88673
PeptideAtlasiO88673
PRIDEiO88673

PTM databases

iPTMnetiO88673
PhosphoSitePlusiO88673

Expressioni

Gene expression databases

BgeeiENSMUSG00000025357 Expressed in 282 organ(s), highest expression level in lymph node
CleanExiMM_DGKA
ExpressionAtlasiO88673 baseline and differential
GenevisibleiO88673 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi199049, 1 interactor
STRINGi10090.ENSMUSP00000026414

Structurei

3D structure databases

ProteinModelPortaliO88673
SMRiO88673
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 146EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini156 – 191EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini368 – 501DAGKcPROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri206 – 254Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri270 – 320Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000252931
HOVERGENiHBG051345
InParanoidiO88673
KOiK00901
OMAiPPWAIYP
OrthoDBiEOG091G04A5
PhylomeDBiO88673
TreeFamiTF313104

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 1 hit
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF14513 DAG_kinase_N, 1 hit
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O88673-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKEKGLISP EDFAQLQKYI EYSTKRVSDV LKVFDDGEMN RFCQGDAIGY
60 70 80 90 100
LGFEQFMKMY LEMEEVPHHL CWALFWSFHT SQVAAEKTKS KANVICLSDV
110 120 130 140 150
YCYFTLLEGG RPEDKLEFTF KLYDMDRNGI LDSTEVEKII LQMMRVAEYL
160 170 180 190 200
DWDVSELRPI LQEMMREMDQ DGSGSVSLDE WVRAGATTVP LLVLLGMDVT
210 220 230 240 250
MKDDGNHIWR PKRFTRLVYC NLCEQSISLG KQGLSCNFCK YIVHDHCAMK
260 270 280 290 300
AQPCEVSTYA KSRKDIGVQS HLWVRGGCHS GRCDRCQKKI RTYHSLTGLH
310 320 330 340 350
CVWCHLEIHD DCLQAVGPEC DCGLLRDHIL PPCSIYPSVL VSGQECKHKT
360 370 380 390 400
TDDTSLCTPE AFRIEPVSNT HPLLVFINLK SGGKQGQSVL WKFQYILNPR
410 420 430 440 450
QVFDLKDGPE PGLRFFKDVP QFRILVCGGD GTVGWVLETI DKANFATVPP
460 470 480 490 500
VAVLPLGTGN DLARCLRWGR GYEGENLRKI LKDIELSKVV YLDRWFLEVI
510 520 530 540 550
PQQNGEKSDP VPSQIINNYF SIGVDASIAH RFHLMREKYP EKFNSRMKNK
560 570 580 590 600
LWYFEFATSE SIFSTCKKLE ESVTVEICGK LLDLSDLSLE GIAVLNIPST
610 620 630 640 650
HGGSNLWGDT KRPHGDTCEI NQALGSAAKI ITDPDILKTC VPDMSDKRLE
660 670 680 690 700
VVGIEGAIEM GQIYTRLKSA GHRLAKCSEI TFQTTKTLPM QIDGEPWMQA
710 720 730
PCTIKITHKN QMPMLMGPPS NSYNFFGFWS
Length:730
Mass (Da):82,762
Last modified:March 27, 2002 - v2
Checksum:iA7808E4F8FD83350
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
R4I5B2R4I5B2_MOUSE
Diacylglycerol kinase alpha
Dgka
253Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti317G → V in AAC33483 (Ref. 1) Curated1
Sequence conflicti392K → N in AAC33483 (Ref. 1) Curated1
Sequence conflicti398N → D in AAC33483 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085219 mRNA Translation: AAC33483.1
BC006713 mRNA Translation: AAH06713.1
CCDSiCCDS24290.1
RefSeqiNP_058091.2, NM_016811.2
XP_011241653.1, XM_011243351.1
XP_011241662.1, XM_011243360.1
XP_017169275.1, XM_017313786.1
XP_017169276.1, XM_017313787.1
XP_017169277.1, XM_017313788.1
UniGeneiMm.291235

Genome annotation databases

EnsembliENSMUST00000026414; ENSMUSP00000026414; ENSMUSG00000025357
GeneIDi13139
KEGGimmu:13139
UCSCiuc007hoc.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085219 mRNA Translation: AAC33483.1
BC006713 mRNA Translation: AAH06713.1
CCDSiCCDS24290.1
RefSeqiNP_058091.2, NM_016811.2
XP_011241653.1, XM_011243351.1
XP_011241662.1, XM_011243360.1
XP_017169275.1, XM_017313786.1
XP_017169276.1, XM_017313787.1
XP_017169277.1, XM_017313788.1
UniGeneiMm.291235

3D structure databases

ProteinModelPortaliO88673
SMRiO88673
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199049, 1 interactor
STRINGi10090.ENSMUSP00000026414

PTM databases

iPTMnetiO88673
PhosphoSitePlusiO88673

Proteomic databases

EPDiO88673
MaxQBiO88673
PaxDbiO88673
PeptideAtlasiO88673
PRIDEiO88673

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026414; ENSMUSP00000026414; ENSMUSG00000025357
GeneIDi13139
KEGGimmu:13139
UCSCiuc007hoc.1 mouse

Organism-specific databases

CTDi1606
MGIiMGI:102952 Dgka

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000252931
HOVERGENiHBG051345
InParanoidiO88673
KOiK00901
OMAiPPWAIYP
OrthoDBiEOG091G04A5
PhylomeDBiO88673
TreeFamiTF313104

Enzyme and pathway databases

BRENDAi2.7.1.107 3474
ReactomeiR-MMU-114508 Effects of PIP2 hydrolysis

Miscellaneous databases

ChiTaRSiDgka mouse
PROiPR:O88673
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025357 Expressed in 282 organ(s), highest expression level in lymph node
CleanExiMM_DGKA
ExpressionAtlasiO88673 baseline and differential
GenevisibleiO88673 MM

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 1 hit
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF14513 DAG_kinase_N, 1 hit
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 1 hit
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiDGKA_MOUSE
AccessioniPrimary (citable) accession number: O88673
Secondary accession number(s): Q922X2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: March 27, 2002
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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