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Protein

GTP-binding protein RAD

Gene

Rrad

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 105GTPBy similarity8
Nucleotide bindingi203 – 206GTPBy similarity4

GO - Molecular functioni

  • calmodulin binding Source: UniProtKB-KW
  • GTPase activity Source: InterPro
  • GTP binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding protein RAD
Gene namesi
Name:Rrad
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1930943 Rrad

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show increased susceptibility to thoracic transverse aortic constriction (TAC)-induced cardiac hypertrophy. TAC-induced CAMKII phosphorylation in the heart is also significantly increased.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105S → A: Loss of interaction with CACNB2. QT prolongation and ventricular arrhythmias, caused by the augmentation of L-type Ca(2+) channels in the heart. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001224791 – 308GTP-binding protein RADAdd BLAST308

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24Omega-N-methylarginineCombined sources1
Modified residuei26PhosphoserineCombined sources1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiO88667
MaxQBiO88667
PaxDbiO88667
PRIDEiO88667

PTM databases

iPTMnetiO88667
PhosphoSitePlusiO88667

Expressioni

Gene expression databases

CleanExiMM_RRAD

Interactioni

Subunit structurei

Interacts with Calmodulin preferentially in the inactive, GDP-bound form. Interacts with CAMK2D (By similarity). Interacts with CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiO88667, 1 interactor
MINTiO88667
STRINGi10090.ENSMUSP00000034351

Structurei

3D structure databases

ProteinModelPortaliO88667
SMRiO88667
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 297Calmodulin-bindingBy similarityAdd BLAST20

Sequence similaritiesi

Belongs to the small GTPase superfamily. RGK family.Curated

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
HOGENOMiHOG000246961
HOVERGENiHBG104899
InParanoidiO88667
PhylomeDBiO88667

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR028869 RAD
IPR017358 RGK
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PTHR24070:SF74 PTHR24070:SF74, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
PIRSFiPIRSF038017 GTP-binding_GEM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O88667-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTLNGGSGAS GSRGAGGRER DRRRGSTPWG PAPPLHRRSM PVDERDLQAA
60 70 80 90 100
LAPGSLATTA AGTRTQGQRL DWPEGSSDSL SSGGSGSEEG VYKVLLLGAP
110 120 130 140 150
GVGKSALARI FGGIEDGPEA EAAGHTYDRS ITVDGEEASL LVYDIWEEDG
160 170 180 190 200
GCWLPGHCMA MGDAYVIVYS ITDKGSFEKA SELRVQLRRA RQTDDVPIIL
210 220 230 240 250
VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ
260 270 280 290 300
IRLRRDSKED NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK

SCHDLSVL
Length:308
Mass (Da):33,279
Last modified:November 1, 1998 - v1
Checksum:i698F0A3BCE605D53
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q6PGA2Q6PGA2_MOUSE
GTP-binding protein
Rrad mCG_23403
307Annotation score:
A0A1D5RLT1A0A1D5RLT1_MOUSE
GTP-binding protein RAD
Rrad
100Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF084466 mRNA Translation: AAC33133.1
UniGeneiMm.29467

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF084466 mRNA Translation: AAC33133.1
UniGeneiMm.29467

3D structure databases

ProteinModelPortaliO88667
SMRiO88667
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88667, 1 interactor
MINTiO88667
STRINGi10090.ENSMUSP00000034351

PTM databases

iPTMnetiO88667
PhosphoSitePlusiO88667

Proteomic databases

EPDiO88667
MaxQBiO88667
PaxDbiO88667
PRIDEiO88667

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1930943 Rrad

Phylogenomic databases

eggNOGiKOG0395 Eukaryota
COG1100 LUCA
HOGENOMiHOG000246961
HOVERGENiHBG104899
InParanoidiO88667
PhylomeDBiO88667

Miscellaneous databases

PROiPR:O88667
SOURCEiSearch...

Gene expression databases

CleanExiMM_RRAD

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR028869 RAD
IPR017358 RGK
IPR001806 Small_GTPase
IPR020849 Small_GTPase_Ras-type
PANTHERiPTHR24070 PTHR24070, 1 hit
PTHR24070:SF74 PTHR24070:SF74, 1 hit
PfamiView protein in Pfam
PF00071 Ras, 1 hit
PIRSFiPIRSF038017 GTP-binding_GEM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51421 RAS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRAD_MOUSE
AccessioniPrimary (citable) accession number: O88667
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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