UniProtKB - O88667 (RAD_MOUSE)
Protein
GTP-binding protein RAD
Gene
Rrad
Organism
Mus musculus (Mouse)
Status
Functioni
May play an important role in cardiac antiarrhythmia via the strong suppression of voltage-gated L-type Ca2+ currents. Regulates voltage-dependent L-type calcium channel subunit alpha-1C trafficking to the cell membrane. Inhibits cardiac hypertrophy through the calmodulin-dependent kinase II (CaMKII) pathway. Inhibits phosphorylation and activation of CAMK2D.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 98 – 105 | GTPBy similarity | 8 | |
Nucleotide bindingi | 203 – 206 | GTPBy similarity | 4 |
GO - Molecular functioni
- calcium channel regulator activity Source: GO_Central
- calmodulin binding Source: UniProtKB-KW
- GTPase activity Source: InterPro
- GTP binding Source: GO_Central
GO - Biological processi
- negative regulation of cell growth Source: MGI
- negative regulation of high voltage-gated calcium channel activity Source: InterPro
- signal transduction Source: InterPro
Keywordsi
Molecular function | Calmodulin-binding |
Ligand | GTP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: GTP-binding protein RAD |
Gene namesi | Name:Rrad |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1930943, Rrad |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Plasma Membrane
- plasma membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Mice show increased susceptibility to thoracic transverse aortic constriction (TAC)-induced cardiac hypertrophy. TAC-induced CAMKII phosphorylation in the heart is also significantly increased.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 105 | S → A: Loss of interaction with CACNB2. QT prolongation and ventricular arrhythmias, caused by the augmentation of L-type Ca(2+) channels in the heart. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000122479 | 1 – 308 | GTP-binding protein RADAdd BLAST | 308 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 24 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 26 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | O88667 |
MaxQBi | O88667 |
PaxDbi | O88667 |
PRIDEi | O88667 |
PTM databases
iPTMneti | O88667 |
PhosphoSitePlusi | O88667 |
Interactioni
Subunit structurei
Interacts with Calmodulin preferentially in the inactive, GDP-bound form.
Interacts with CAMK2D (By similarity).
Interacts with CACNB2. Interaction with CACNB2 regulates the trafficking of CACNA1C to the cell membrane.
By similarity1 PublicationGO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
IntActi | O88667, 1 interactor |
MINTi | O88667 |
STRINGi | 10090.ENSMUSP00000034351 |
Miscellaneous databases
RNActi | O88667, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 278 – 297 | Calmodulin-bindingBy similarityAdd BLAST | 20 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
InParanoidi | O88667 |
PhylomeDBi | O88667 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028869, RAD IPR017358, RGK IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR45775:SF3, PTHR45775:SF3, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PIRSFi | PIRSF038017, GTP-binding_GEM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
O88667-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTLNGGSGAS GSRGAGGRER DRRRGSTPWG PAPPLHRRSM PVDERDLQAA
60 70 80 90 100
LAPGSLATTA AGTRTQGQRL DWPEGSSDSL SSGGSGSEEG VYKVLLLGAP
110 120 130 140 150
GVGKSALARI FGGIEDGPEA EAAGHTYDRS ITVDGEEASL LVYDIWEEDG
160 170 180 190 200
GCWLPGHCMA MGDAYVIVYS ITDKGSFEKA SELRVQLRRA RQTDDVPIIL
210 220 230 240 250
VGNKSDLVRS REVSVDEGRA CAVVFDCKFI ETSAALHHNV QALFEGVVRQ
260 270 280 290 300
IRLRRDSKED NARRQAGTRR RESLGKKAKR FLGRIVARNS RKMAFRAKSK
SCHDLSVL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ6PGA2 | Q6PGA2_MOUSE | GTP-binding protein | Rrad | 307 | Annotation score: | ||
A0A1D5RLT1 | A0A1D5RLT1_MOUSE | GTP-binding protein RAD | Rrad | 100 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084466 mRNA Translation: AAC33133.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084466 mRNA Translation: AAC33133.1 |
3D structure databases
SMRi | O88667 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O88667, 1 interactor |
MINTi | O88667 |
STRINGi | 10090.ENSMUSP00000034351 |
PTM databases
iPTMneti | O88667 |
PhosphoSitePlusi | O88667 |
Proteomic databases
EPDi | O88667 |
MaxQBi | O88667 |
PaxDbi | O88667 |
PRIDEi | O88667 |
Organism-specific databases
MGIi | MGI:1930943, Rrad |
Phylogenomic databases
eggNOGi | KOG0395, Eukaryota |
InParanoidi | O88667 |
PhylomeDBi | O88667 |
Miscellaneous databases
PROi | PR:O88667 |
RNActi | O88667, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR028869, RAD IPR017358, RGK IPR001806, Small_GTPase IPR020849, Small_GTPase_Ras-type |
PANTHERi | PTHR45775:SF3, PTHR45775:SF3, 1 hit |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
PIRSFi | PIRSF038017, GTP-binding_GEM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51421, RAS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAD_MOUSE | |
Accessioni | O88667Primary (citable) accession number: O88667 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 7, 2003 |
Last sequence update: | November 1, 1998 | |
Last modified: | August 12, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families