UniProtKB - O88665 (BRD7_MOUSE)
Bromodomain-containing protein 7
Brd7
Functioni
Acts both as coactivator and as corepressor. May play a role in chromatin remodeling. Transcriptional corepressor that down-regulates the expression of target genes. Binds to target promoters, leading to increased histone H3 acetylation at 'Lys-9' (H3K9ac). Binds to the ESR1 promoter. Recruits BRCA1 and POU2F1 to the ESR1 promoter. Coactivator for TP53-mediated activation of transcription of a set of target genes. Required for TP53-mediated cell-cycle arrest in response to oncogene activation. Promotes acetylation of TP53 at 'Lys-382', and thereby promotes efficient recruitment of TP53 to target promoters. Inhibits cell cycle progression from G1 to S phase (By similarity).
Activator of the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. Induces dephosphorylation of GSK3B at 'Tyr-216'. Down-regulates TRIM24-mediated activation of transcriptional activation by AR.
By similarity3 PublicationsGO - Molecular functioni
- histone binding Source: MGI
- lysine-acetylated histone binding Source: UniProtKB
- p53 binding Source: UniProtKB
- transcription cis-regulatory region binding Source: MGI
- transcription coactivator activity Source: UniProtKB
- transcription corepressor activity Source: UniProtKB
GO - Biological processi
- cell cycle Source: UniProtKB-KW
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of G1/S transition of mitotic cell cycle Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of histone acetylation Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of transcription by RNA polymerase II Source: MGI
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Biological process | Cell cycle, Transcription, Transcription regulation, Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-MMU-6804758, Regulation of TP53 Activity through Acetylation |
Names & Taxonomyi
Protein namesi | Recommended name: Bromodomain-containing protein 7Alternative name(s): 75 kDa bromodomain protein |
Gene namesi | Name:Brd7 Synonyms:Bp75 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1349766, Brd7 |
VEuPathDBi | HostDB:ENSMUSG00000031660 |
Subcellular locationi
Nucleus
- Nucleus 2 Publications
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000227665 | 1 – 651 | Bromodomain-containing protein 7Add BLAST | 651 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 21 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 127 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 186 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 197 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 201 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 212 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 241 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 279 | PhosphoserineCombined sources | 1 | |
Modified residuei | 289 | PhosphoserineBy similarity | 1 | |
Cross-linki | 305 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 328 | N6-acetyllysineCombined sources | 1 | |
Cross-linki | 344 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 380 | PhosphoserineBy similarity | 1 | |
Cross-linki | 389 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 475 | PhosphoserineCombined sources | 1 | |
Modified residuei | 482 | PhosphoserineCombined sources | 1 | |
Modified residuei | 483 | PhosphoserineCombined sources | 1 | |
Modified residuei | 621 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O88665 |
jPOSTi | O88665 |
MaxQBi | O88665 |
PaxDbi | O88665 |
PeptideAtlasi | O88665 |
PRIDEi | O88665 |
ProteomicsDBi | 265378 |
PTM databases
iPTMneti | O88665 |
PhosphoSitePlusi | O88665 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000031660, Expressed in spleen and 331 other tissues |
Genevisiblei | O88665, MM |
Interactioni
Subunit structurei
Interacts with IRF2 and HNRPUL1 (By similarity).
Interacts (via N-terminus) with TP53.
Interacts (via C-terminus) with EP300.
Interacts with BRCA1.
Interacts (via bromo domain) with histone H3 (via N-terminus) acetylated at 'Lys-14' (H3K14ac). Has low affinity for histone H3 acetylated at 'Lys-9' (H3K9ac). Has the highest affinity for histone H3 that is acetylated both at 'Lys-9' (H3K9ac) and at 'Lys-14' (H3K14ac). Has very low affinity for non-acetylated histone H3.
Interacts (via bromo domain) with histone H4 (via N-terminus) acetylated at 'Lys-8' (H3K8ac) (in vitro) (By similarity).
Interacts with TRIM24, PTPN13 and DVL1.
Identified in a complex with SMARCA4/BRG1, SMARCC1/BAF155, SMARCE1/BAF57, DPF2/BAF45D and ARID2, subunits of the SWI/SNF-B (PBAF) chromatin remodeling complex.
By similarity4 PublicationsBinary interactionsi
O88665
With | #Exp. | IntAct |
---|---|---|
Pik3r1 [P26450] | 11 | EBI-643930,EBI-641764 |
Pik3r2 [O08908] | 4 | EBI-643930,EBI-643570 |
Ptpn13 [Q64512] | 3 | EBI-643930,EBI-4284057 |
GO - Molecular functioni
- histone binding Source: MGI
- lysine-acetylated histone binding Source: UniProtKB
- p53 binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 205094, 7 interactors |
ComplexPortali | CPX-1248, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6A variant CPX-1250, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B variant |
IntActi | O88665, 4 interactors |
MINTi | O88665 |
STRINGi | 10090.ENSMUSP00000034085 |
Miscellaneous databases
RNActi | O88665, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 148 – 218 | BromoPROSITE-ProRule annotationAdd BLAST | 71 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 34 – 103 | DisorderedSequence analysisAdd BLAST | 70 | |
Regioni | 252 – 316 | DisorderedSequence analysisAdd BLAST | 65 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 536 – 567 | Sequence analysisAdd BLAST | 32 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 65 – 96 | Nuclear localization signalBy similarityAdd BLAST | 32 |
Keywords - Domaini
Bromodomain, Coiled coilPhylogenomic databases
eggNOGi | KOG1828, Eukaryota |
GeneTreei | ENSGT00950000183170 |
HOGENOMi | CLU_020704_0_1_1 |
InParanoidi | O88665 |
OMAi | GEDSSCW |
OrthoDBi | 439339at2759 |
PhylomeDBi | O88665 |
TreeFami | TF106439 |
Family and domain databases
Gene3Di | 1.20.920.10, 1 hit |
InterProi | View protein in InterPro IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR021900, DUF3512 |
Pfami | View protein in Pfam PF00439, Bromodomain, 1 hit PF12024, DUF3512, 1 hit |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 1 hit |
SUPFAMi | SSF47370, SSF47370, 1 hit |
PROSITEi | View protein in PROSITE PS50014, BROMODOMAIN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGKKHKKHKS DRHFYEEYVE KPLKLVLKVG GSEVTELSTG SSGHDSSLFE
60 70 80 90 100
DRSDHDKHKD RKRKKRKKGE KQAPGEEKGR KRRRVKEDKK KRDRDRAENE
110 120 130 140 150
VDRDLQCHVP IRLDLPPEKP LTSSLAKQEE VEQTPLQEAL NQLMRQLQRK
160 170 180 190 200
DPSAFFSFPV TDFIAPGYSM IIKHPMDFST MKEKIKNNDY QSIEELKDNF
210 220 230 240 250
KLMCTNAMIY NKPETIYYKA AKKLLHSGMK ILSQERIQSL KQSIDFMSDL
260 270 280 290 300
QKTRKQKERT DACQSGEDSG CWQREREDSG DAETQAFRSP AKDNKRKDKD
310 320 330 340 350
VLEDKWRSSN SEREHEQIER VVQESGGKLT RRLANSQCEF ERRKPDGTTT
360 370 380 390 400
LGLLHPVDPI VGEPGYCPVR LGMTTGRLQS GVNTLQGFKE DKRNRVTPVL
410 420 430 440 450
YLNYGPYSSY APHYDSTFAN ISKDDSDLIY STYGEDSDLP NNFSISEFLA
460 470 480 490 500
TCQDYPYVMA DSLLDVLTKG GHSRSLQDLD MSSPEDEGQT RALDTAKEAE
510 520 530 540 550
ITQIEPTGRL ESSSQDRLTA LQAVTTFGAP AEVFDSEEAE VFQRKLDETT
560 570 580 590 600
RLLRELQEAQ NERLSTRPPP NMICLLGPSY REMYLAEQVT NNLKELTQQV
610 620 630 640 650
TPGDVVSIHG VRKAMGISVP SPIVGNSFVD LTGECEEPKE TSTAECGPDA
S
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | D → G in BAE23823 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084259 mRNA Translation: AAC33302.1 AK004429 mRNA Translation: BAB23299.1 AK138934 mRNA Translation: BAE23823.1 Different initiation. AK142758 mRNA Translation: BAE25187.1 |
CCDSi | CCDS22510.1 |
RefSeqi | NP_036177.1, NM_012047.2 XP_017168329.1, XM_017312840.1 |
Genome annotation databases
Ensembli | ENSMUST00000034085; ENSMUSP00000034085; ENSMUSG00000031660 |
GeneIDi | 26992 |
KEGGi | mmu:26992 |
UCSCi | uc009mrn.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084259 mRNA Translation: AAC33302.1 AK004429 mRNA Translation: BAB23299.1 AK138934 mRNA Translation: BAE23823.1 Different initiation. AK142758 mRNA Translation: BAE25187.1 |
CCDSi | CCDS22510.1 |
RefSeqi | NP_036177.1, NM_012047.2 XP_017168329.1, XM_017312840.1 |
3D structure databases
BMRBi | O88665 |
SMRi | O88665 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 205094, 7 interactors |
ComplexPortali | CPX-1248, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6A variant CPX-1250, Polybromo-associated SWI/SNF ATP-dependent chromatin remodeling complex, ACTL6B variant |
IntActi | O88665, 4 interactors |
MINTi | O88665 |
STRINGi | 10090.ENSMUSP00000034085 |
Chemistry databases
ChEMBLi | CHEMBL3822348 |
PTM databases
iPTMneti | O88665 |
PhosphoSitePlusi | O88665 |
Proteomic databases
EPDi | O88665 |
jPOSTi | O88665 |
MaxQBi | O88665 |
PaxDbi | O88665 |
PeptideAtlasi | O88665 |
PRIDEi | O88665 |
ProteomicsDBi | 265378 |
Protocols and materials databases
Antibodypediai | 14527, 224 antibodies from 32 providers |
DNASUi | 26992 |
Genome annotation databases
Ensembli | ENSMUST00000034085; ENSMUSP00000034085; ENSMUSG00000031660 |
GeneIDi | 26992 |
KEGGi | mmu:26992 |
UCSCi | uc009mrn.2, mouse |
Organism-specific databases
CTDi | 29117 |
MGIi | MGI:1349766, Brd7 |
VEuPathDBi | HostDB:ENSMUSG00000031660 |
Phylogenomic databases
eggNOGi | KOG1828, Eukaryota |
GeneTreei | ENSGT00950000183170 |
HOGENOMi | CLU_020704_0_1_1 |
InParanoidi | O88665 |
OMAi | GEDSSCW |
OrthoDBi | 439339at2759 |
PhylomeDBi | O88665 |
TreeFami | TF106439 |
Enzyme and pathway databases
Reactomei | R-MMU-6804758, Regulation of TP53 Activity through Acetylation |
Miscellaneous databases
BioGRID-ORCSi | 26992, 3 hits in 69 CRISPR screens |
ChiTaRSi | Brd7, mouse |
PROi | PR:O88665 |
RNActi | O88665, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031660, Expressed in spleen and 331 other tissues |
Genevisiblei | O88665, MM |
Family and domain databases
Gene3Di | 1.20.920.10, 1 hit |
InterProi | View protein in InterPro IPR001487, Bromodomain IPR036427, Bromodomain-like_sf IPR021900, DUF3512 |
Pfami | View protein in Pfam PF00439, Bromodomain, 1 hit PF12024, DUF3512, 1 hit |
PRINTSi | PR00503, BROMODOMAIN |
SMARTi | View protein in SMART SM00297, BROMO, 1 hit |
SUPFAMi | SSF47370, SSF47370, 1 hit |
PROSITEi | View protein in PROSITE PS50014, BROMODOMAIN_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | BRD7_MOUSE | |
Accessioni | O88665Primary (citable) accession number: O88665 Secondary accession number(s): Q3UQ56, Q3UU06, Q9CT78 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 21, 2006 |
Last sequence update: | November 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot