UniProtKB - O88662 (EMP2_MOUSE)
Protein
Epithelial membrane protein 2
Gene
Emp2
Organism
Mus musculus (Mouse)
Status
Functioni
Functions as a key regulator of cell membrane composition by regulating proteins surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation (PubMed:17609206, PubMed:14978215). Facilitates surface trafficking and the formation of lipid rafts bearing GPI-anchor proteins (PubMed:14978215). Regulates surface expression of MHC1 and ICAM1 proteins increasing susceptibility to T-cell mediated cytotoxicity (PubMed:12763482). Regulates the plasma membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion (PubMed:12189152). Also regulates many processes through PTK2. Regulates blood vessel endothelial cell migration and angiogenesis by regulating VEGF protein expression through PTK2 activation (By similarity). Regulates cell migration and cell contraction through PTK2 and SRC activation (By similarity). Regulates focal adhesion density, F-actin conformation and cell adhesion capacity through interaction with PTK2 (By similarity). Positively regulates cell proliferation (By similarity). Plays a role during cell death and cell blebbing (By similarity). Promotes angiogenesis and vasculogenesis through induction of VEGFA via a HIF1A-dependent pathway (By similarity). Also plays a role in embryo implantation by regulating surface trafficking of integrin heterodimer ITGA5-ITGB3 (PubMed:16487956, PubMed:16216233). May play a role in glomerular filtration (By similarity).By similarity6 Publications
GO - Molecular functioni
- integrin binding Source: UniProtKB
- kinase binding Source: MGI
- protein kinase binding Source: MGI
GO - Biological processi
- actin filament organization Source: UniProtKB
- actin-mediated cell contraction Source: UniProtKB
- activation of protein kinase activity Source: UniProtKB
- bleb assembly Source: UniProtKB
- blood vessel endothelial cell migration Source: UniProtKB
- cell adhesion Source: UniProtKB
- cell death Source: UniProtKB
- cell-matrix adhesion Source: UniProtKB
- cell migration Source: UniProtKB
- early endosome to late endosome transport Source: UniProtKB
- embryo implantation Source: UniProtKB
- membrane raft assembly Source: UniProtKB
- positive regulation of cell-matrix adhesion Source: MGI
- positive regulation of cell population proliferation Source: UniProtKB
- positive regulation of integrin-mediated signaling pathway Source: MGI
- protein localization to cell surface Source: UniProtKB
- protein localization to plasma membrane Source: UniProtKB
- regulation of angiogenesis Source: UniProtKB
- regulation of cell-matrix adhesion Source: UniProtKB
- regulation of endothelial cell migration Source: UniProtKB
- regulation of glomerular filtration Source: UniProtKB
- regulation of kinase activity Source: UniProtKB
- regulation of vasculogenesis Source: UniProtKB
- T cell mediated cytotoxicity Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: Epithelial membrane protein 2Short name: EMP-2 Alternative name(s): Protein XMP |
Gene namesi | Name:Emp2 Synonyms:Xmp |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1098726, Emp2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Golgi apparatus
- Golgi apparatus membrane Curated3 Publications; Multi-pass membrane protein Sequence analysis
Plasma membrane
- Cell membrane 1 Publication
- Apical cell membrane 1 Publication
Other locations
- Membrane raft 2 Publications
Note: Localizes in cytoplasm, foot processes and cell bodies of podocytes and nucleus of endothelial cells of kidney (By similarity). Localizes to the apical cell surface in the luminal epithelium and glandular epithelium (PubMed:16487956). Colocalized with ITGB1 and GPI-anchor proteins on plasma membrane (PubMed:12189152) (PubMed:14978215).By similarity3 Publications
Cytosol
- cytosol Source: GOC
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- Golgi membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- apical plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- apical part of cell Source: UniProtKB
- cell surface Source: UniProtKB
- cytoplasm Source: UniProtKB
- integral component of membrane Source: UniProtKB-KW
- membrane raft Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 1 – 21 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 72 – 92 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 100 – 120 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 148 – 168 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Golgi apparatus, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000164659 | 1 – 172 | Epithelial membrane protein 2Add BLAST | 172 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 44 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 47 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
PaxDbi | O88662 |
PRIDEi | O88662 |
ProteomicsDBi | 275749 |
PTM databases
GlyGeni | O88662, 2 sites |
PhosphoSitePlusi | O88662 |
Expressioni
Tissue specificityi
Expressed in both the endometrial epithelial and stromal cells during the embryo implantation period (PubMed:16487956). Expressed in the corneal epithelium, the sclera, the nerve fiber layer of the inner retina and retinal ganglion cell layer, the nonpigmented epithelium of the ciliary body and the optic nerve (PubMed:12710941).2 Publications
Gene expression databases
Bgeei | ENSMUSG00000022505, Expressed in lung and 308 other tissues |
ExpressionAtlasi | O88662, baseline and differential |
Genevisiblei | O88662, MM |
Interactioni
Subunit structurei
Interacts with PTK2; regulates PTK2 activation and localization (By similarity).
Interacts with ITGB3; regulates the levels of the heterodimer ITGA5-ITGB3 integrin surface expression (By similarity).
Interacts with P2RX7 (via C-terminus) (By similarity).
Interacts with ITGB1; the interaction may be direct or indirect and ITGB1 has a heterodimer form (PubMed:12189152).
By similarity1 PublicationGO - Molecular functioni
- integrin binding Source: UniProtKB
- kinase binding Source: MGI
- protein kinase binding Source: MGI
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077466 |
Miscellaneous databases
RNActi | O88662, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the PMP-22/EMP/MP20 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502RYYE, Eukaryota |
GeneTreei | ENSGT00950000182696 |
HOGENOMi | CLU_138632_0_0_1 |
InParanoidi | O88662 |
OMAi | GSYGYSF |
OrthoDBi | 1345659at2759 |
PhylomeDBi | O88662 |
TreeFami | TF330414 |
Family and domain databases
InterProi | View protein in InterPro IPR003933, EMP-2 IPR004031, PMP22/EMP/MP20/Claudin IPR004032, PMP22_EMP_MP20 |
PANTHERi | PTHR10671:SF32, PTHR10671:SF32, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01453, EPMEMFAMILY PR01455, EPMEMPROT2 |
PROSITEi | View protein in PROSITE PS01221, PMP22_1, 1 hit PS01222, PMP22_2, 1 hit |
i Sequence
Sequence statusi: Complete.
O88662-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLVILAFIIV FHIVSTALLF ISTIDNAWWV GDSFSADLWR VCTNSTNCTE
60 70 80 90 100
INELTGPEAF EGYSVMQAVQ ATMILSTILS CISFLIFLLQ LFRLKQGERF
110 120 130 140 150
VLTSIIQLMS CLCVMIGASI YTDRRQDLHQ QNRKLYYLLQ EGSYGYSFIL
160 170
AWVAFAFTFI SGLMYMILRK RK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083876 mRNA Translation: AAC33108.1 |
CCDSi | CCDS37250.1 |
RefSeqi | NP_031955.2, NM_007929.2 |
Genome annotation databases
Ensembli | ENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505 |
GeneIDi | 13731 |
KEGGi | mmu:13731 |
UCSCi | uc007ydj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF083876 mRNA Translation: AAC33108.1 |
CCDSi | CCDS37250.1 |
RefSeqi | NP_031955.2, NM_007929.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000077466 |
PTM databases
GlyGeni | O88662, 2 sites |
PhosphoSitePlusi | O88662 |
Proteomic databases
PaxDbi | O88662 |
PRIDEi | O88662 |
ProteomicsDBi | 275749 |
Protocols and materials databases
Antibodypediai | 11432, 145 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505 |
GeneIDi | 13731 |
KEGGi | mmu:13731 |
UCSCi | uc007ydj.1, mouse |
Organism-specific databases
CTDi | 2013 |
MGIi | MGI:1098726, Emp2 |
Phylogenomic databases
eggNOGi | ENOG502RYYE, Eukaryota |
GeneTreei | ENSGT00950000182696 |
HOGENOMi | CLU_138632_0_0_1 |
InParanoidi | O88662 |
OMAi | GSYGYSF |
OrthoDBi | 1345659at2759 |
PhylomeDBi | O88662 |
TreeFami | TF330414 |
Miscellaneous databases
BioGRID-ORCSi | 13731, 1 hit in 55 CRISPR screens |
ChiTaRSi | Emp2, mouse |
PROi | PR:O88662 |
RNActi | O88662, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022505, Expressed in lung and 308 other tissues |
ExpressionAtlasi | O88662, baseline and differential |
Genevisiblei | O88662, MM |
Family and domain databases
InterProi | View protein in InterPro IPR003933, EMP-2 IPR004031, PMP22/EMP/MP20/Claudin IPR004032, PMP22_EMP_MP20 |
PANTHERi | PTHR10671:SF32, PTHR10671:SF32, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01453, EPMEMFAMILY PR01455, EPMEMPROT2 |
PROSITEi | View protein in PROSITE PS01221, PMP22_1, 1 hit PS01222, PMP22_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | EMP2_MOUSE | |
Accessioni | O88662Primary (citable) accession number: O88662 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | November 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families