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Protein

Epithelial membrane protein 2

Gene

Emp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as a key regulator of cell membrane composition by regulating proteins surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation (PubMed:17609206, PubMed:14978215). Facilitates surface trafficking and the formation of lipid rafts bearing GPI-anchor proteins (PubMed:14978215). Regulates surface expression of MHC1 and ICAM1 proteins increasing susceptibility to T-cell mediated cytotoxicity (PubMed:12763482). Regulates the plasma membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion (PubMed:12189152). Also regulates many processes through PTK2. Regulates blood vessel endothelial cell migration and angiogenesis by regulating VEGF protein expression through PTK2 activation (By similarity). Regulates cell migration and cell contraction through PTK2 and SRC activation (By similarity). Regulates focal adhesion density, F-actin conformation and cell adhesion capacity through interaction with PTK2 (By similarity). Positively regulates cell proliferation (By similarity). Plays a role during cell death and cell blebbing (By similarity). Promotes angiogenesis and vasculogenesis through induction of VEGFA via a HIF1A-dependent pathway (By similarity). Also plays a role in embryo implantation by regulating surface trafficking of integrin heterodimer ITGA5-ITGB3 (PubMed:16487956, PubMed:16216233). May play a role in glomerular filtration (By similarity).By similarity6 Publications

GO - Molecular functioni

  • integrin binding Source: UniProtKB
  • kinase binding Source: MGI
  • protein kinase binding Source: MGI

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Epithelial membrane protein 2
Short name:
EMP-2
Alternative name(s):
Protein XMP
Gene namesi
Name:Emp2
Synonyms:Xmp
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1098726 Emp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei148 – 168HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001646591 – 172Epithelial membrane protein 2Add BLAST172

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi44N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi47N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO88662
PRIDEiO88662

PTM databases

PhosphoSitePlusiO88662

Expressioni

Tissue specificityi

Expressed in both the endometrial epithelial and stromal cells during the embryo implantation period (PubMed:16487956). Expressed in the corneal epithelium, the sclera, the nerve fiber layer of the inner retina and retinal ganglion cell layer, the nonpigmented epithelium of the ciliary body and the optic nerve (PubMed:12710941).2 Publications

Gene expression databases

BgeeiENSMUSG00000022505 Expressed in 294 organ(s), highest expression level in lung
CleanExiMM_EMP2
ExpressionAtlasiO88662 baseline and differential
GenevisibleiO88662 MM

Interactioni

Subunit structurei

Interacts with PTK2; regulates PTK2 activation and localization (By similarity). Interacts with ITGB3; regulates the levels of the heterodimer ITGA5-ITGB3 integrin surface expression (By similarity). Interacts with P2RX7 (via C-terminus) (By similarity). Interacts with ITGB1; the interaction may be direct or indirect and ITGB1 has a heterodimer form (PubMed:12189152).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077466

Structurei

3D structure databases

ProteinModelPortaliO88662
SMRiO88662
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PMP-22/EMP/MP20 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH06 Eukaryota
ENOG4111QXD LUCA
GeneTreeiENSGT00510000046328
HOGENOMiHOG000059542
HOVERGENiHBG001690
InParanoidiO88662
OMAiNNTNCTE
OrthoDBiEOG091G0RF4
PhylomeDBiO88662
TreeFamiTF330414

Family and domain databases

InterProiView protein in InterPro
IPR003933 EMP-2
IPR004031 PMP22/EMP/MP20/Claudin
IPR004032 PMP22_EMP_MP20
PANTHERiPTHR10671:SF32 PTHR10671:SF32, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01453 EPMEMFAMILY
PR01455 EPMEMPROT2
PROSITEiView protein in PROSITE
PS01221 PMP22_1, 1 hit
PS01222 PMP22_2, 1 hit

Sequencei

Sequence statusi: Complete.

O88662-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLVILAFIIV FHIVSTALLF ISTIDNAWWV GDSFSADLWR VCTNSTNCTE
60 70 80 90 100
INELTGPEAF EGYSVMQAVQ ATMILSTILS CISFLIFLLQ LFRLKQGERF
110 120 130 140 150
VLTSIIQLMS CLCVMIGASI YTDRRQDLHQ QNRKLYYLLQ EGSYGYSFIL
160 170
AWVAFAFTFI SGLMYMILRK RK
Length:172
Mass (Da):19,747
Last modified:November 1, 1998 - v1
Checksum:i6110C073BC75FB6D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083876 mRNA Translation: AAC33108.1
CCDSiCCDS37250.1
RefSeqiNP_031955.2, NM_007929.2
UniGeneiMm.246009

Genome annotation databases

EnsembliENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505
GeneIDi13731
KEGGimmu:13731
UCSCiuc007ydj.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083876 mRNA Translation: AAC33108.1
CCDSiCCDS37250.1
RefSeqiNP_031955.2, NM_007929.2
UniGeneiMm.246009

3D structure databases

ProteinModelPortaliO88662
SMRiO88662
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000077466

PTM databases

PhosphoSitePlusiO88662

Proteomic databases

PaxDbiO88662
PRIDEiO88662

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505
GeneIDi13731
KEGGimmu:13731
UCSCiuc007ydj.1 mouse

Organism-specific databases

CTDi2013
MGIiMGI:1098726 Emp2

Phylogenomic databases

eggNOGiENOG410IH06 Eukaryota
ENOG4111QXD LUCA
GeneTreeiENSGT00510000046328
HOGENOMiHOG000059542
HOVERGENiHBG001690
InParanoidiO88662
OMAiNNTNCTE
OrthoDBiEOG091G0RF4
PhylomeDBiO88662
TreeFamiTF330414

Miscellaneous databases

PROiPR:O88662
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022505 Expressed in 294 organ(s), highest expression level in lung
CleanExiMM_EMP2
ExpressionAtlasiO88662 baseline and differential
GenevisibleiO88662 MM

Family and domain databases

InterProiView protein in InterPro
IPR003933 EMP-2
IPR004031 PMP22/EMP/MP20/Claudin
IPR004032 PMP22_EMP_MP20
PANTHERiPTHR10671:SF32 PTHR10671:SF32, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01453 EPMEMFAMILY
PR01455 EPMEMPROT2
PROSITEiView protein in PROSITE
PS01221 PMP22_1, 1 hit
PS01222 PMP22_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEMP2_MOUSE
AccessioniPrimary (citable) accession number: O88662
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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