Emp2

Functionⁱ

Functions as a key regulator of cell membrane composition by regulating proteins surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation (PubMed:17609206, PubMed:14978215). Facilitates surface trafficking and the formation of lipid rafts bearing GPI-anchor proteins (PubMed:14978215). Regulates surface expression of MHC1 and ICAM1 proteins increasing susceptibility to T-cell mediated cytotoxicity (PubMed:12763482). Regulates the plasma membrane expression of the integrin heterodimers ITGA6-ITGB1, ITGA5-ITGB3 and ITGA5-ITGB1 resulting in modulation of cell-matrix adhesion (PubMed:12189152). Also regulates many processes through PTK2. Regulates blood vessel endothelial cell migration and angiogenesis by regulating VEGF protein expression through PTK2 activation (By similarity). Regulates cell migration and cell contraction through PTK2 and SRC activation (By similarity). Regulates focal adhesion density, F-actin conformation and cell adhesion capacity through interaction with PTK2 (By similarity). Positively regulates cell proliferation (By similarity). Plays a role during cell death and cell blebbing (By similarity). Promotes angiogenesis and vasculogenesis through induction of VEGFA via a HIF1A-dependent pathway (By similarity). Also plays a role in embryo implantation by regulating surface trafficking of integrin heterodimer ITGA5-ITGB3 (PubMed:16487956, PubMed:16216233). May play a role in glomerular filtration (By similarity).By similarity6 Publications

GO - Molecular functionⁱ

integrin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 12189152
kinase binding Source: MGI
protein kinase binding Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

actin filament organization Source: UniProtKB
actin-mediated cell contraction Source: UniProtKB
activation of protein kinase activity Source: UniProtKB
bleb assembly Source: UniProtKB
blood vessel endothelial cell migration Source: UniProtKB
cell adhesion Source: UniProtKB
cell death Source: UniProtKB
cell-matrix adhesion Source: UniProtKB
cell migration Source: UniProtKB
early endosome to late endosome transport
Source: UniProtKB
Inferred from direct assayⁱ
- 18400107
embryo implantation
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 16487956
membrane raft assembly
Source: UniProtKB
Inferred from direct assayⁱ
- 14978215
positive regulation of cell-matrix adhesion
Source: MGI
Inferred from sequence orthologyⁱ
- 16216233
positive regulation of cell proliferation Source: UniProtKB
positive regulation of integrin-mediated signaling pathway
Source: MGI
Inferred from mutant phenotypeⁱ
- 16216233
protein localization to cell surface Source: UniProtKB
protein localization to plasma membrane
Source: UniProtKB
Inferred from direct assayⁱ
- 12189152
regulation of angiogenesis Source: UniProtKB
regulation of cell-matrix adhesion
Source: UniProtKB
Inferred from direct assayⁱ
- 12189152
regulation of endothelial cell migration Source: UniProtKB
regulation of glomerular filtration Source: UniProtKB
regulation of kinase activity Source: UniProtKB
regulation of vasculogenesis Source: UniProtKB
T cell mediated cytotoxicity
Source: UniProtKB
Inferred from direct assayⁱ
- 12763482

View the complete GO annotation on QuickGO ...

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Epithelial membrane protein 2 Short name: EMP-2 Alternative name(s): Protein XMP
Gene namesⁱ	Name:Emp2 Synonyms:Xmp
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:1098726 Emp2

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	1 – 21	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	72 – 92	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	100 – 120	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	148 – 168	HelicalSequence analysisAdd BLAST	21

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000164659	1 – 172	Epithelial membrane protein 2Add BLAST		172

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Glycosylationⁱ	44	N-linked (GlcNAc...) asparagineSequence analysis		1
Glycosylationⁱ	47	N-linked (GlcNAc...) asparagineSequence analysis		1

Keywords - PTMⁱ

Glycoprotein

Proteomic databases

PaxDbⁱ	O88662
PRIDEⁱ	O88662

PTM databases

PhosphoSitePlusⁱ	O88662

PhosphoSitePlusⁱ

O88662

Expressionⁱ

Tissue specificityⁱ

Expressed in both the endometrial epithelial and stromal cells during the embryo implantation period (PubMed:16487956). Expressed in the corneal epithelium, the sclera, the nerve fiber layer of the inner retina and retinal ganglion cell layer, the nonpigmented epithelium of the ciliary body and the optic nerve (PubMed:12710941).2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000022505 Expressed in 294 organ(s), highest expression level in lung
CleanExⁱ	MM_EMP2
ExpressionAtlasⁱ	O88662 baseline and differential
Genevisibleⁱ	O88662 MM

Interactionⁱ

Subunit structureⁱ

Interacts with PTK2; regulates PTK2 activation and localization (By similarity). Interacts with ITGB3; regulates the levels of the heterodimer ITGA5-ITGB3 integrin surface expression (By similarity). Interacts with P2RX7 (via C-terminus) (By similarity). Interacts with ITGB1; the interaction may be direct or indirect and ITGB1 has a heterodimer form (PubMed:12189152).By similarity1 Publication

GO - Molecular functionⁱ

integrin binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 12189152
kinase binding Source: MGI
protein kinase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000077466

STRINGⁱ

10090.ENSMUSP00000077466

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O88662
SMRⁱ	O88662
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the PMP-22/EMP/MP20 family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IH06 Eukaryota ENOG4111QXD LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00510000046328
HOGENOMⁱ	HOG000059542
HOVERGENⁱ	HBG001690
InParanoidⁱ	O88662
OMAⁱ	NNTNCTE
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0RF4
PhylomeDBⁱ	O88662
TreeFamⁱ	TF330414

Family and domain databases

InterProⁱ	View protein in InterPro IPR003933 EMP-2 IPR004031 PMP22/EMP/MP20/Claudin IPR004032 PMP22_EMP_MP20
PANTHERⁱ	PTHR10671:SF32 PTHR10671:SF32, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00822 PMP22_Claudin, 1 hit
PRINTSⁱ	PR01453 EPMEMFAMILY PR01455 EPMEMPROT2
PROSITEⁱ	View protein in PROSITE PS01221 PMP22_1, 1 hit PS01222 PMP22_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O88662-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLVILAFIIV FHIVSTALLF ISTIDNAWWV GDSFSADLWR VCTNSTNCTE 
        60         70         80         90        100
INELTGPEAF EGYSVMQAVQ ATMILSTILS CISFLIFLLQ LFRLKQGERF 
       110        120        130        140        150
VLTSIIQLMS CLCVMIGASI YTDRRQDLHQ QNRKLYYLLQ EGSYGYSFIL 
       160        170 
AWVAFAFTFI SGLMYMILRK RK

Length:172

Mass (Da):19,747

Last modified:November 1, 1998 - v1

Checksum:ⁱ6110C073BC75FB6D

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF083876 mRNA Translation: AAC33108.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS37250.1
NCBI Reference Sequences More...RefSeqⁱ	NP_031955.2, NM_007929.2
UniGeneⁱ	Mm.246009

Genome annotation databases

Ensemblⁱ	ENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505
GeneIDⁱ	13731
KEGGⁱ	mmu:13731
UCSC genome browser More...UCSCⁱ	uc007ydj.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O88662	Epithelial membrane protein 2		MOUSE		172	UniRef100_O88662
Epithelial membrane protein 2-like protein (Fragment)		LATJA		165
Epithelial membrane protein 2 (Fragment)		wild yak		143
UPI0003C03C82		wild yak		140

Protein	Similar proteins		Species	Score	Length	Source
O88662	Epithelial membrane protein 2		RAT		172	UniRef90_O88662
Epithelial membrane protein 2		MOUSE		172
Epithelial membrane protein 2-like protein (Fragment)		LATJA		165
Epithelial membrane protein 2 (Fragment)		wild yak		143
Epithelial membrane protein 2		Okinawa island spiny rat		172
+5

Protein	Similar proteins		Species	Score	Length	Source
O88662	Epithelial membrane protein 2		HUMAN		167	UniRef50_P54851
Epithelial membrane protein 2		PANTR		167
Epithelial membrane protein 2		RAT		172
Epithelial membrane protein 2		BOVIN		167
Epithelial membrane protein 2		NOMLE		167
+198

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF083876 mRNA Translation: AAC33108.1
CCDSⁱ	CCDS37250.1
RefSeqⁱ	NP_031955.2, NM_007929.2
UniGeneⁱ	Mm.246009

3D structure databases

ProteinModelPortalⁱ	O88662
SMRⁱ	O88662
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000077466

STRINGⁱ

10090.ENSMUSP00000077466

PTM databases

PhosphoSitePlusⁱ	O88662

PhosphoSitePlusⁱ

O88662

Proteomic databases

PaxDbⁱ	O88662
PRIDEⁱ	O88662

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000078357; ENSMUSP00000077466; ENSMUSG00000022505
GeneIDⁱ	13731
KEGGⁱ	mmu:13731
UCSCⁱ	uc007ydj.1 mouse

Organism-specific databases

CTDⁱ	2013
MGIⁱ	MGI:1098726 Emp2

Phylogenomic databases

eggNOGⁱ	ENOG410IH06 Eukaryota ENOG4111QXD LUCA
GeneTreeⁱ	ENSGT00510000046328
HOGENOMⁱ	HOG000059542
HOVERGENⁱ	HBG001690
InParanoidⁱ	O88662
OMAⁱ	NNTNCTE
OrthoDBⁱ	EOG091G0RF4
PhylomeDBⁱ	O88662
TreeFamⁱ	TF330414

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:O88662
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000022505 Expressed in 294 organ(s), highest expression level in lung
CleanExⁱ	MM_EMP2
ExpressionAtlasⁱ	O88662 baseline and differential
Genevisibleⁱ	O88662 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR003933 EMP-2 IPR004031 PMP22/EMP/MP20/Claudin IPR004032 PMP22_EMP_MP20
PANTHERⁱ	PTHR10671:SF32 PTHR10671:SF32, 1 hit
Pfamⁱ	View protein in Pfam PF00822 PMP22_Claudin, 1 hit
PRINTSⁱ	PR01453 EPMEMFAMILY PR01455 EPMEMPROT2
PROSITEⁱ	View protein in PROSITE PS01221 PMP22_1, 1 hit PS01222 PMP22_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	EMP2_MOUSE
Accessionⁱ	O88662Primary (citable) accession number: O88662
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
	Last sequence update:	November 1, 1998
	Last modified:	November 7, 2018
	This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O88662 (EMP2_MOUSE)

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Epithelial membrane protein 2

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Golgi apparatus

Plasma membrane

Nucleus

Other locations

Cytosol

Golgi apparatus

Nucleus

Plasma Membrane

Other locations

Topology

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ