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Protein

Proteinase-activated receptor 4

Gene

F2rl3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for activated thrombin or trypsin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation.

GO - Molecular functioni

  • G protein-coupled receptor activity Source: GO_Central
  • thrombin-activated receptor activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBlood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-416476 G alpha (q) signalling events
R-MMU-456926 Thrombin signalling through proteinase activated receptors (PARs)

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 4
Short name:
PAR-4
Alternative name(s):
Coagulation factor II receptor-like 3
Thrombin receptor-like 3
Gene namesi
Name:F2rl3
Synonyms:Par4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1298207 F2rl3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini60 – 94ExtracellularSequence analysisAdd BLAST35
Transmembranei95 – 115Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini116 – 120CytoplasmicSequence analysis5
Transmembranei121 – 141Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini142 – 162ExtracellularSequence analysisAdd BLAST21
Transmembranei163 – 183Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini184 – 203CytoplasmicSequence analysisAdd BLAST20
Transmembranei204 – 224Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini225 – 255ExtracellularSequence analysisAdd BLAST31
Transmembranei256 – 276Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini277 – 295CytoplasmicSequence analysisAdd BLAST19
Transmembranei296 – 316Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini317 – 331ExtracellularSequence analysisAdd BLAST15
Transmembranei332 – 355Helical; Name=7Sequence analysisAdd BLAST24
Topological domaini356 – 396CytoplasmicSequence analysisAdd BLAST41

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000001276417 – 59Removed for receptor activationBy similarityAdd BLAST43
ChainiPRO_000001276560 – 396Proteinase-activated receptor 4Add BLAST337

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi161 ↔ 240PROSITE-ProRule annotation

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei59 – 60Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiO88634
PaxDbiO88634
PRIDEiO88634

PTM databases

PhosphoSitePlusiO88634

Expressioni

Tissue specificityi

Highly expressed in the spleen. Slight expression in the heart, lung, skeletal muscle and kidney. No detectable expression in brain, liver or testis. Also detected in platelets.

Gene expression databases

BgeeiENSMUSG00000050147 Expressed in 49 organ(s), highest expression level in left lobe of liver
CleanExiMM_F2RL3
GenevisibleiO88634 MM

Interactioni

Protein-protein interaction databases

DIPiDIP-42480N
IntActiO88634, 2 interactors
MINTiO88634
STRINGi10090.ENSMUSP00000054426

Structurei

Secondary structure

1396
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO88634
SMRiO88634
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88634

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi374 – 377Poly-Ser4

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFSN Eukaryota
ENOG410YD35 LUCA
GeneTreeiENSGT00920000148955
HOGENOMiHOG000116291
HOVERGENiHBG096365
InParanoidiO88634
KOiK04236
OMAiCHDALPL
OrthoDBiEOG091G0C2F
TreeFamiTF350010

Family and domain databases

CDDicd15372 7tmA_PAR4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR003944 Prot_act_rcpt_4
IPR003912 Protea_act_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR01428 PROTEASEAR
PR01430 PROTEASEAR4
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O88634-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MCWPLLYPLV LGLSISLAEG IQTPSIYDDV ESTRGSHEGP LGPTVELKEP
60 70 80 90 100
KSSDKPNPRG YPGKFCANDS DTLELPASSQ ALLLGWVPTR LVPALYGLVV
110 120 130 140 150
AVGLPANGLA LWVLATRVPR LPSTILLMNL AVADLLLALV LPPRLAYHLR
160 170 180 190 200
GQRWPFGEAA CRVATAALYG HMYGSVLLLA AVSLDRYLAL VHPLRARALR
210 220 230 240 250
GQRLTTGLCL VAWLSAATLA LPLTLHRQTF RLAGSDRMLC HDALPLTEQT
260 270 280 290 300
SHWRPAFICL AVLGCFVPLL AMGLCYGATL RALAANGQRY SHALRLTALV
310 320 330 340 350
LFSAVASFTP SNVLLVLHYS NPSPEAWGNL YGAYVPSLAL STLNSCVDPF
360 370 380 390
IYYYVSHEFR EKVRAMLCRQ PEASSSSQAS REAGSRGTAI CSSTLL
Length:396
Mass (Da):42,786
Last modified:July 27, 2011 - v2
Checksum:iDC10502E7AAE1B86
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88P → S in AAC28861 (PubMed:9722561).Curated1
Sequence conflicti128M → T in AAC28861 (PubMed:9722561).Curated1
Sequence conflicti135L → S in AAC28861 (PubMed:9722561).Curated1
Sequence conflicti141L → P in AAC28861 (PubMed:9722561).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080215 mRNA Translation: AAC28861.1
AK036427 mRNA Translation: BAC29423.1
AK153720 mRNA Translation: BAE32155.1
AK156729 mRNA Translation: BAE33823.1
CH466525 Genomic DNA Translation: EDL10818.1
CCDSiCCDS22419.1
RefSeqiNP_032001.2, NM_007975.4
UniGeneiMm.12948

Genome annotation databases

EnsembliENSMUST00000058099; ENSMUSP00000054426; ENSMUSG00000050147
GeneIDi14065
KEGGimmu:14065
UCSCiuc009mgr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080215 mRNA Translation: AAC28861.1
AK036427 mRNA Translation: BAC29423.1
AK153720 mRNA Translation: BAE32155.1
AK156729 mRNA Translation: BAE33823.1
CH466525 Genomic DNA Translation: EDL10818.1
CCDSiCCDS22419.1
RefSeqiNP_032001.2, NM_007975.4
UniGeneiMm.12948

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PV9X-ray3.50C51-76[»]
ProteinModelPortaliO88634
SMRiO88634
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-42480N
IntActiO88634, 2 interactors
MINTiO88634
STRINGi10090.ENSMUSP00000054426

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiO88634

Proteomic databases

MaxQBiO88634
PaxDbiO88634
PRIDEiO88634

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000058099; ENSMUSP00000054426; ENSMUSG00000050147
GeneIDi14065
KEGGimmu:14065
UCSCiuc009mgr.1 mouse

Organism-specific databases

CTDi9002
MGIiMGI:1298207 F2rl3

Phylogenomic databases

eggNOGiENOG410IFSN Eukaryota
ENOG410YD35 LUCA
GeneTreeiENSGT00920000148955
HOGENOMiHOG000116291
HOVERGENiHBG096365
InParanoidiO88634
KOiK04236
OMAiCHDALPL
OrthoDBiEOG091G0C2F
TreeFamiTF350010

Enzyme and pathway databases

ReactomeiR-MMU-375276 Peptide ligand-binding receptors
R-MMU-416476 G alpha (q) signalling events
R-MMU-456926 Thrombin signalling through proteinase activated receptors (PARs)

Miscellaneous databases

EvolutionaryTraceiO88634
PROiPR:O88634
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000050147 Expressed in 49 organ(s), highest expression level in left lobe of liver
CleanExiMM_F2RL3
GenevisibleiO88634 MM

Family and domain databases

CDDicd15372 7tmA_PAR4, 1 hit
InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR003944 Prot_act_rcpt_4
IPR003912 Protea_act_rcpt
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR01428 PROTEASEAR
PR01430 PROTEASEAR4
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPAR4_MOUSE
AccessioniPrimary (citable) accession number: O88634
Secondary accession number(s): Q8BZ77
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 142 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
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Main funding by: National Institutes of Health

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