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Protein

Forkhead box protein H1

Gene

Foxh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi64 – 163Fork-headPROSITE-ProRule annotationAdd BLAST100

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1181150 Signaling by NODAL
R-MMU-1502540 Signaling by Activin

Names & Taxonomyi

Protein namesi
Recommended name:
Forkhead box protein H1
Alternative name(s):
Forkhead activin signal transducer 1
Short name:
Fast-1
Forkhead activin signal transducer 2
Short name:
Fast-2
Gene namesi
Name:Foxh1
Synonyms:Fast1, Fast2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1347465 Foxh1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000918431 – 401Forkhead box protein H1Add BLAST401

Proteomic databases

PaxDbiO88621
PRIDEiO88621

PTM databases

iPTMnetiO88621
PhosphoSitePlusiO88621

Expressioni

Developmental stagei

Expressed predominantly throughout the epiblast before gastrulation and declines as development progresses.1 Publication

Gene expression databases

BgeeiENSMUSG00000033837 Expressed in 133 organ(s), highest expression level in epiblast (generic)
CleanExiMM_FOXH1
ExpressionAtlasiO88621 baseline and differential
GenevisibleiO88621 MM

Interactioni

Subunit structurei

Interacts with the MH2 domains of SMAD2 and SMAD3.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
GscQ025912EBI-7457430,EBI-7457485

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199598, 7 interactors
CORUMiO88621
IntActiO88621, 3 interactors
MINTiO88621
STRINGi10090.ENSMUSP00000036591

Structurei

3D structure databases

ProteinModelPortaliO88621
SMRiO88621
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni307 – 390SMAD-interaction domain (SID)Add BLAST84

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi311 – 315Fast/FoxH1 motif 1 (FM1)5
Motifi321 – 327Fast/FoxH1 motif 2 (FM2)7
Motifi363 – 384SMAD interaction motif (SIM)Add BLAST22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi187 – 194Poly-Pro8
Compositional biasi243 – 246Poly-Ser4

Domaini

The FM region is required for binding SMAD2/SMAD4 complexes. FM2 is more effective than FM1 and only interacts with phosphorylated SMAD2 that is in an activated SMAD complex (By similarity).By similarity

Phylogenomic databases

eggNOGiKOG2294 Eukaryota
COG5025 LUCA
GeneTreeiENSGT00930000150834
HOGENOMiHOG000112630
HOVERGENiHBG051647
InParanoidiO88621
KOiK09400
OMAiMIAMVIQ
OrthoDBiEOG091G0X2S
PhylomeDBiO88621
TreeFamiTF350620

Family and domain databases

CDDicd00059 FH, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001766 Fork_head_dom
IPR030456 TF_fork_head_CS_2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00250 Forkhead, 1 hit
PRINTSiPR00053 FORKHEAD
SMARTiView protein in SMART
SM00339 FH, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00658 FORK_HEAD_2, 1 hit
PS50039 FORK_HEAD_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform Long (identifier: O88621-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MASGWDLAST YTPTTPSPQL ALAPAQGYLP CMGPRDNSQL RPPEAESLSK
60 70 80 90 100
TPKRRKKRYL RHDKPPYTYL AMIALVIQAA PFRRLKLAQI IRQVQAVFPF
110 120 130 140 150
FRDDYEGWKD SIRHNLSSNR CFHKVPKDPA KPQAKGNFWA VDVSLIPAEA
160 170 180 190 200
LRLQNTALCR RWQNRGTHRA FAKDLSPYVL HGQPYQPPSP PPPPREGFSI
210 220 230 240 250
KSLLGDPGKE STWPQHPGLP GQSTAAQAGT LSKGEEGMGT GPSSSSETPL
260 270 280 290 300
WPLCSLPGPT IIEGESSQGE VIRPSPVTPD QGSWPLHLLE DSADSRGVPR
310 320 330 340 350
RGSRASLWGQ LPTSYLPIYT PNVVMPLATL PTTSCPQCPS SASPAYWSVG
360 370 380 390 400
TESQGSQDLL CDLDSLFQGV PPNKSIYDVW VSHPRDLAAP APGWLLSWYS

M
Length:401
Mass (Da):44,001
Last modified:November 1, 1998 - v1
Checksum:iFB11DC9779DF1454
GO
Isoform Short (identifier: O88621-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     89-92: Missing.

Note: Fails to bind DNA and cannot confer activin response element responsiveness.
Show »
Length:397
Mass (Da):43,490
Checksum:i3FBEF9C838B20567
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti167T → A in AAD55949 (PubMed:10349617).Curated1
Sequence conflicti207P → L in AAD14683 (Ref. 4) Curated1
Sequence conflicti215Q → K in AAD14683 (Ref. 4) Curated1
Sequence conflicti220P → L in AAD14683 (Ref. 4) Curated1
Sequence conflicti223S → N in AAD55949 (PubMed:10349617).Curated1
Sequence conflicti237G → R in AAD55949 (PubMed:10349617).Curated1
Sequence conflicti330L → I in AAD55949 (PubMed:10349617).Curated1
Sequence conflicti334S → C in AAD55949 (PubMed:10349617).Curated1
Sequence conflicti340S → F in AAD55949 (PubMed:10349617).Curated1
Sequence conflicti366L → F in AAD55949 (PubMed:10349617).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00154289 – 92Missing in isoform Short. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069303 mRNA Translation: AAC79808.1
AF079514 mRNA Translation: AAD12486.1
AF177770 mRNA Translation: AAD55949.1
AF110506 mRNA Translation: AAD14683.1
CCDSiCCDS27584.1 [O88621-1]
RefSeqiNP_032015.1, NM_007989.4 [O88621-1]
XP_006520499.1, XM_006520436.3 [O88621-2]
UniGeneiMm.42011

Genome annotation databases

EnsembliENSMUST00000037824; ENSMUSP00000036591; ENSMUSG00000033837 [O88621-1]
GeneIDi14106
KEGGimmu:14106
UCSCiuc007wlm.2 mouse [O88621-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069303 mRNA Translation: AAC79808.1
AF079514 mRNA Translation: AAD12486.1
AF177770 mRNA Translation: AAD55949.1
AF110506 mRNA Translation: AAD14683.1
CCDSiCCDS27584.1 [O88621-1]
RefSeqiNP_032015.1, NM_007989.4 [O88621-1]
XP_006520499.1, XM_006520436.3 [O88621-2]
UniGeneiMm.42011

3D structure databases

ProteinModelPortaliO88621
SMRiO88621
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199598, 7 interactors
CORUMiO88621
IntActiO88621, 3 interactors
MINTiO88621
STRINGi10090.ENSMUSP00000036591

PTM databases

iPTMnetiO88621
PhosphoSitePlusiO88621

Proteomic databases

PaxDbiO88621
PRIDEiO88621

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037824; ENSMUSP00000036591; ENSMUSG00000033837 [O88621-1]
GeneIDi14106
KEGGimmu:14106
UCSCiuc007wlm.2 mouse [O88621-1]

Organism-specific databases

CTDi8928
MGIiMGI:1347465 Foxh1

Phylogenomic databases

eggNOGiKOG2294 Eukaryota
COG5025 LUCA
GeneTreeiENSGT00930000150834
HOGENOMiHOG000112630
HOVERGENiHBG051647
InParanoidiO88621
KOiK09400
OMAiMIAMVIQ
OrthoDBiEOG091G0X2S
PhylomeDBiO88621
TreeFamiTF350620

Enzyme and pathway databases

ReactomeiR-MMU-1181150 Signaling by NODAL
R-MMU-1502540 Signaling by Activin

Miscellaneous databases

PROiPR:O88621
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033837 Expressed in 133 organ(s), highest expression level in epiblast (generic)
CleanExiMM_FOXH1
ExpressionAtlasiO88621 baseline and differential
GenevisibleiO88621 MM

Family and domain databases

CDDicd00059 FH, 1 hit
Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR001766 Fork_head_dom
IPR030456 TF_fork_head_CS_2
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PfamiView protein in Pfam
PF00250 Forkhead, 1 hit
PRINTSiPR00053 FORKHEAD
SMARTiView protein in SMART
SM00339 FH, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00658 FORK_HEAD_2, 1 hit
PS50039 FORK_HEAD_3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFOXH1_MOUSE
AccessioniPrimary (citable) accession number: O88621
Secondary accession number(s): Q9QZL5, Q9R241
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 155 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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