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Protein

Voltage-dependent calcium channel gamma-2 subunit

Gene

Cacng2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state (By similarity).By similarity

GO - Molecular functioni

  • channel regulator activity Source: GO_Central
  • ionotropic glutamate receptor binding Source: MGI
  • voltage-gated calcium channel activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionCalcium channel, Ion channel, Voltage-gated channel
Biological processCalcium transport, Ion transport, Transport
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-112308 Presynaptic depolarization and calcium channel opening
R-MMU-399719 Trafficking of AMPA receptors
R-MMU-5576892 Phase 0 - rapid depolarisation
R-MMU-5576893 Phase 2 - plateau phase
R-MMU-5682910 LGI-ADAM interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent calcium channel gamma-2 subunit
Alternative name(s):
Neuronal voltage-gated calcium channel gamma-2 subunit
Stargazin
Transmembrane AMPAR regulatory protein gamma-2
Short name:
TARP gamma-2
Gene namesi
Name:Cacng2
Synonyms:Stg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1316660 Cacng2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Transmembranei104 – 124HelicalSequence analysisAdd BLAST21
Transmembranei134 – 154HelicalSequence analysisAdd BLAST21
Transmembranei182 – 202HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell junction, Membrane, Synapse, Synaptosome

Pathology & Biotechi

Involvement in diseasei

Defects in Cacng2 cause the stargazer (stg) phenotype. Stg mice have spike-wave seizures characteristic of absence epilepsy, with accompanying defects in the cerebellum and inner ear.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi321T → A: Abolishes phosphorylation. 1 Publication1
Mutagenesisi321T → D or E: No interaction with DLG1 and DLG4. 1 Publication1
Mutagenesisi323V → A: No interaction with DLG1 and DLG4. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001646741 – 323Voltage-dependent calcium channel gamma-2 subunitAdd BLAST323

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei253PhosphoserineBy similarity1
Modified residuei271PhosphotyrosineCombined sources1
Modified residuei321Phosphothreonine; by PKA1 Publication1

Post-translational modificationi

Phosphorylation of Thr-321 by PKA impairs interaction with DLG1 and DLG4.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO88602
PeptideAtlasiO88602
PRIDEiO88602

PTM databases

iPTMnetiO88602
PhosphoSitePlusiO88602

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiENSMUSG00000019146 Expressed in 162 organ(s), highest expression level in lumbar subsegment of spinal cord
ExpressionAtlasiO88602 baseline and differential
GenevisibleiO88602 MM

Interactioni

Subunit structurei

The L-type calcium channel is composed of five subunits: alpha-1, alpha-2/delta, beta and gamma. Interacts with the PDZ domains of DLG4/PSD-95 and DLG1/SAP97. May interact with GOPC. Acts as an auxiliary subunit for AMPA-selective glutamate receptors (AMPARs). Found in a complex with GRIA1, GRIA2, GRIA3, GRIA4, CNIH2, CNIH3, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with GRIA1 and GRIA2 (By similarity). Interacts with MPP2.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Gria1P238182EBI-770326,EBI-445486

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198444, 4 interactors
DIPiDIP-32457N
ELMiO88602
IntActiO88602, 4 interactors
STRINGi10090.ENSMUSP00000019290

Structurei

Secondary structure

1323
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO88602
SMRiO88602
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PMP-22/EMP/MP20 family. CACNG subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFNQ Eukaryota
ENOG4110ISU LUCA
GeneTreeiENSGT00550000074547
HOGENOMiHOG000233440
HOVERGENiHBG003682
InParanoidiO88602
KOiK04867
OMAiNARATDY
OrthoDBiEOG091G0D2Z
PhylomeDBiO88602
TreeFamiTF327980

Family and domain databases

InterProiView protein in InterPro
IPR004031 PMP22/EMP/MP20/Claudin
IPR005422 VDCC_g2su
IPR008368 VDCC_gsu
PANTHERiPTHR12107:SF1 PTHR12107:SF1, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01792 VDCCGAMMA
PR01602 VDCCGAMMA2

Sequencei

Sequence statusi: Complete.

O88602-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLFDRGVQM LLTTVGAFAA FSLMTIAVGT DYWLYSRGVC KTKSVSENET
60 70 80 90 100
SKKNEEVMTH SGLWRTCCLE GNFKGLCKQI DHFPEDADYE ADTAEYFLRA
110 120 130 140 150
VRASSIFPIL SVILLFMGGL CIAASEFYKT RHNIILSAGI FFVSAGLSNI
160 170 180 190 200
IGIIVYISAN AGDPSKSDSK KNSYSYGWSF YFGALSFIIA EMVGVLAVHM
210 220 230 240 250
FIDRHKQLRA TARATDYLQA SAITRIPSYR YRYQRRSRSS SRSTEPSHSR
260 270 280 290 300
DASPVGVKGF NTLPSTEISM YTLSRDPLKA ATTPTATYNS DRDNSFLQVH
310 320
NCIQKDSKDS LHANTANRRT TPV
Length:323
Mass (Da):35,895
Last modified:November 1, 1998 - v1
Checksum:iAA9D475606A0FBA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077739 mRNA Translation: AAC40201.1
CCDSiCCDS27608.1
RefSeqiNP_031609.1, NM_007583.2
UniGeneiMm.277338
Mm.400802

Genome annotation databases

EnsembliENSMUST00000019290; ENSMUSP00000019290; ENSMUSG00000019146
GeneIDi12300
KEGGimmu:12300
UCSCiuc007wop.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF077739 mRNA Translation: AAC40201.1
CCDSiCCDS27608.1
RefSeqiNP_031609.1, NM_007583.2
UniGeneiMm.277338
Mm.400802

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JXTX-ray1.50C/D318-323[»]
4X3HX-ray2.40B225-233[»]
5KBSelectron microscopy8.70A/B/C/D2-208[»]
5KBTelectron microscopy6.40A/B/C/D2-208[»]
5KBUelectron microscopy7.80A/B/C/D2-208[»]
5WEOelectron microscopy4.20A/B/C/D2-208[»]
ProteinModelPortaliO88602
SMRiO88602
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198444, 4 interactors
DIPiDIP-32457N
ELMiO88602
IntActiO88602, 4 interactors
STRINGi10090.ENSMUSP00000019290

PTM databases

iPTMnetiO88602
PhosphoSitePlusiO88602

Proteomic databases

PaxDbiO88602
PeptideAtlasiO88602
PRIDEiO88602

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019290; ENSMUSP00000019290; ENSMUSG00000019146
GeneIDi12300
KEGGimmu:12300
UCSCiuc007wop.2 mouse

Organism-specific databases

CTDi10369
MGIiMGI:1316660 Cacng2

Phylogenomic databases

eggNOGiENOG410IFNQ Eukaryota
ENOG4110ISU LUCA
GeneTreeiENSGT00550000074547
HOGENOMiHOG000233440
HOVERGENiHBG003682
InParanoidiO88602
KOiK04867
OMAiNARATDY
OrthoDBiEOG091G0D2Z
PhylomeDBiO88602
TreeFamiTF327980

Enzyme and pathway databases

ReactomeiR-MMU-112308 Presynaptic depolarization and calcium channel opening
R-MMU-399719 Trafficking of AMPA receptors
R-MMU-5576892 Phase 0 - rapid depolarisation
R-MMU-5576893 Phase 2 - plateau phase
R-MMU-5682910 LGI-ADAM interactions

Miscellaneous databases

ChiTaRSiSgta mouse
PROiPR:O88602
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019146 Expressed in 162 organ(s), highest expression level in lumbar subsegment of spinal cord
ExpressionAtlasiO88602 baseline and differential
GenevisibleiO88602 MM

Family and domain databases

InterProiView protein in InterPro
IPR004031 PMP22/EMP/MP20/Claudin
IPR005422 VDCC_g2su
IPR008368 VDCC_gsu
PANTHERiPTHR12107:SF1 PTHR12107:SF1, 1 hit
PfamiView protein in Pfam
PF00822 PMP22_Claudin, 1 hit
PRINTSiPR01792 VDCCGAMMA
PR01602 VDCCGAMMA2
ProtoNetiSearch...

Entry informationi

Entry nameiCCG2_MOUSE
AccessioniPrimary (citable) accession number: O88602
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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