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Protein

Axin-2

Gene

Axin2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Inhibitor of the Wnt signaling pathway. Down-regulates beta-catenin. Probably facilitate the phosphorylation of beta-catenin and APC by GSK3B (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-201681 TCF dependent signaling in response to WNT
R-MMU-4641257 Degradation of AXIN
R-MMU-5689880 Ub-specific processing proteases

Names & Taxonomyi

Protein namesi
Recommended name:
Axin-2
Alternative name(s):
Axin-like protein
Short name:
Axil
Axis inhibition protein 2
Conductin
Gene namesi
Name:Axin2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1270862 Axin2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Chemistry databases

ChEMBLiCHEMBL1255128

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002208961 – 840Axin-2Add BLAST840

Post-translational modificationi

Probably phosphorylated by GSK3B and dephosphorylated by PP2A.By similarity
ADP-ribosylated by tankyrase TNKS and TNKS2. Poly-ADP-ribosylated protein is recognized by RNF146, followed by ubiquitination and subsequent activation of the Wnt signaling pathway (By similarity).By similarity
Ubiquitinated by RNF146 when poly-ADP-ribosylated, leading to its degradation and subsequent activation of the Wnt signaling pathway. Deubiquitinated by USP34, deubiquitinated downstream of beta-catenin stabilization step: deubiquitination is important Wnt signaling to positively regulate beta-catenin (CTNBB1)-mediated transcription (By similarity).By similarity

Keywords - PTMi

ADP-ribosylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO88566
PaxDbiO88566
PRIDEiO88566

PTM databases

iPTMnetiO88566
PhosphoSitePlusiO88566

Expressioni

Gene expression databases

BgeeiENSMUSG00000000142 Expressed in 356 organ(s), highest expression level in primary oocyte
CleanExiMM_AXIN2
ExpressionAtlasiO88566 baseline and differential
GenevisibleiO88566 MM

Interactioni

Subunit structurei

Interacts with glycogen synthase kinase-3 beta (GSK3B) and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Interacts with SMAD7 and RNF111 (By similarity). Interacts with ANKRD6.By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198288, 6 interactors
ComplexPortaliCPX-449 Beta-catenin destruction core complex, variant 3
CPX-452 Beta-catenin destruction core complex, variant 4
CPX-457 Beta-catenin destruction core complex, variant 7
CPX-458 Beta-catenin destruction core complex, variant 8
CORUMiO88566
DIPiDIP-42640N
IntActiO88566, 9 interactors
MINTiO88566
STRINGi10090.ENSMUSP00000051331

Structurei

3D structure databases

ProteinModelPortaliO88566
SMRiO88566
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini81 – 200RGSPROSITE-ProRule annotationAdd BLAST120
Domaini758 – 840DIXPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni327 – 413Interaction with GSK3BBy similarityAdd BLAST87
Regioni413 – 478Interaction with beta-cateninBy similarityAdd BLAST66

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi21 – 30Tankyrase-binding motif10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi469 – 476Poly-His8

Domaini

The tankyrase-binding motif (also named TBD) is required for interaction with tankyrase TNKS and TNKS2.By similarity

Phylogenomic databases

eggNOGiKOG3589 Eukaryota
ENOG410YMJD LUCA
GeneTreeiENSGT00390000010011
HOGENOMiHOG000231228
HOVERGENiHBG004324
InParanoidiO88566
KOiK04385
OMAiKTYIRDS
OrthoDBiEOG091G0ABG
TreeFamiTF315454

Family and domain databases

CDDicd11582 Axin_TNKS_binding, 1 hit
Gene3Di1.10.196.10, 1 hit
3.10.20.380, 1 hit
InterProiView protein in InterPro
IPR014936 Axin_b-cat-bd
IPR032101 Axin_TNKS-bd
IPR001158 DIX
IPR038207 DIX_dom_sf
IPR016137 RGS
IPR036305 RGS_sf
IPR024066 RGS_subdom1/3
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF16646 AXIN1_TNKS_BD, 1 hit
PF08833 Axin_b-cat_bind, 1 hit
PF00778 DIX, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR01301 RGSPROTEIN
SMARTiView protein in SMART
SM00021 DAX, 1 hit
SM00315 RGS, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50841 DIX, 1 hit
PS50132 RGS, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O88566-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSAVLVTLL PDPSSSFRED APRPPVPGEE GETPPCQPSV GKVQSTKPMP
60 70 80 90 100
VSSNARRNED GLGEPEGRAS PDSPLTRWTK SLHSLLGDQD GAYLFRTFLE
110 120 130 140 150
REKCVDTLDF WFACNGFRQM NLKDTKTLRV AKAIYKRYIE NNSVVSKQLK
160 170 180 190 200
PATKTYIRDG IKKQQIGSVM FDQAQTEIQA VMEENAYQVF LTSDIYLEYV
210 220 230 240 250
RSGGENTAYM SNGGLGSLKV LCGYLPTLNE EEEWTCADLK CKLSPTVVGL
260 270 280 290 300
SSKTLRATAS VRSTETAENG FRSFKRSDPV NPYHVGSGYV FAPATSANDS
310 320 330 340 350
ELSSDALTDD SMSMTDSSVD GVPPYRMGSK KQLQREMHRS VKANGQVSLP
360 370 380 390 400
HFPRTHRLPK EMTPVEPAAF AAELISRLEK LKLELESRHS LEERLQQIRE
410 420 430 440 450
DEEKEGSEQA LSSRDGAPVQ HPLALLPSGS YEEDPQTILD DHLSRVLKTP
460 470 480 490 500
GCQSPGVGRY SPRSRSPDHH HQHHHHQQCH TLLPTGGKLP PVAACPLLGG
510 520 530 540 550
KSFLTKQTTK HVHHHYIHHH AVPKTKEEIE AEATQRVRCL CPGGTDYYCY
560 570 580 590 600
SKCKSHPKAP EPLPGEQFCG SRGGTLPKRN AKGTEPGLAL SARDGGMSSA
610 620 630 640 650
AGAPQLPGEE GDRSQDVWQW MLESERQSKS KPHSAQSIRK SYPLESACAA
660 670 680 690 700
PGERVSRHHL LGASGHSRSV ARAHPFTQDP AMPPLTPPNT LAQLEEACRR
710 720 730 740 750
LAEVSKPQKQ RCCVASQQRD RNHSAAGQAG ASPFANPSLA PEDHKEPKKL
760 770 780 790 800
ASVHALQASE LVVTYFFCGE EIPYRRMLKA QSLTLGHFKE QLSKKGNYRY
810 820 830 840
YFKKASDEFA CGAVFEEIWD DETVLPMYEG RILGKVERID
Length:840
Mass (Da):92,906
Last modified:July 27, 2011 - v2
Checksum:i53D7D33128879298
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2ABR0A2ABR0_MOUSE
Axin-2
Axin2
775Annotation score:
A2ABQ9A2ABQ9_MOUSE
Axin-2
Axin2
103Annotation score:
A2ABQ8A2ABQ8_MOUSE
Axin-2
Axin2
98Annotation score:
A2ABQ7A2ABQ7_MOUSE
Axin-2
Axin2
69Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101R → K in AAF22800 (Ref. 2) Curated1
Sequence conflicti474H → Y in AAF22800 (Ref. 2) Curated1
Sequence conflicti484P → S in AAC26047 (PubMed:9554852).Curated1
Sequence conflicti503F → S in AAF22800 (Ref. 2) Curated1
Sequence conflicti603A → G in AAC26047 (PubMed:9554852).Curated1
Sequence conflicti648C → R in AAC26047 (PubMed:9554852).Curated1
Sequence conflicti648C → R in AAF22800 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073788 mRNA Translation: AAC26047.1
AF205889 mRNA Translation: AAF22800.1
AL662893 Genomic DNA Translation: CAM17369.1
CH466558 Genomic DNA Translation: EDL34346.1
BC057338 mRNA Translation: AAH57338.1
CCDSiCCDS25575.1
RefSeqiNP_056547.3, NM_015732.4
XP_006532120.1, XM_006532057.3
XP_006532121.1, XM_006532058.1
XP_006532122.1, XM_006532059.3
XP_006532123.1, XM_006532060.1
UniGeneiMm.71710

Genome annotation databases

EnsembliENSMUST00000052915; ENSMUSP00000051331; ENSMUSG00000000142
GeneIDi12006
KEGGimmu:12006
UCSCiuc007mbu.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF073788 mRNA Translation: AAC26047.1
AF205889 mRNA Translation: AAF22800.1
AL662893 Genomic DNA Translation: CAM17369.1
CH466558 Genomic DNA Translation: EDL34346.1
BC057338 mRNA Translation: AAH57338.1
CCDSiCCDS25575.1
RefSeqiNP_056547.3, NM_015732.4
XP_006532120.1, XM_006532057.3
XP_006532121.1, XM_006532058.1
XP_006532122.1, XM_006532059.3
XP_006532123.1, XM_006532060.1
UniGeneiMm.71710

3D structure databases

ProteinModelPortaliO88566
SMRiO88566
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198288, 6 interactors
ComplexPortaliCPX-449 Beta-catenin destruction core complex, variant 3
CPX-452 Beta-catenin destruction core complex, variant 4
CPX-457 Beta-catenin destruction core complex, variant 7
CPX-458 Beta-catenin destruction core complex, variant 8
CORUMiO88566
DIPiDIP-42640N
IntActiO88566, 9 interactors
MINTiO88566
STRINGi10090.ENSMUSP00000051331

Chemistry databases

ChEMBLiCHEMBL1255128

PTM databases

iPTMnetiO88566
PhosphoSitePlusiO88566

Proteomic databases

MaxQBiO88566
PaxDbiO88566
PRIDEiO88566

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000052915; ENSMUSP00000051331; ENSMUSG00000000142
GeneIDi12006
KEGGimmu:12006
UCSCiuc007mbu.2 mouse

Organism-specific databases

CTDi8313
MGIiMGI:1270862 Axin2

Phylogenomic databases

eggNOGiKOG3589 Eukaryota
ENOG410YMJD LUCA
GeneTreeiENSGT00390000010011
HOGENOMiHOG000231228
HOVERGENiHBG004324
InParanoidiO88566
KOiK04385
OMAiKTYIRDS
OrthoDBiEOG091G0ABG
TreeFamiTF315454

Enzyme and pathway databases

ReactomeiR-MMU-201681 TCF dependent signaling in response to WNT
R-MMU-4641257 Degradation of AXIN
R-MMU-5689880 Ub-specific processing proteases

Miscellaneous databases

PROiPR:O88566
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000142 Expressed in 356 organ(s), highest expression level in primary oocyte
CleanExiMM_AXIN2
ExpressionAtlasiO88566 baseline and differential
GenevisibleiO88566 MM

Family and domain databases

CDDicd11582 Axin_TNKS_binding, 1 hit
Gene3Di1.10.196.10, 1 hit
3.10.20.380, 1 hit
InterProiView protein in InterPro
IPR014936 Axin_b-cat-bd
IPR032101 Axin_TNKS-bd
IPR001158 DIX
IPR038207 DIX_dom_sf
IPR016137 RGS
IPR036305 RGS_sf
IPR024066 RGS_subdom1/3
IPR029071 Ubiquitin-like_domsf
PfamiView protein in Pfam
PF16646 AXIN1_TNKS_BD, 1 hit
PF08833 Axin_b-cat_bind, 1 hit
PF00778 DIX, 1 hit
PF00615 RGS, 1 hit
PRINTSiPR01301 RGSPROTEIN
SMARTiView protein in SMART
SM00021 DAX, 1 hit
SM00315 RGS, 1 hit
SUPFAMiSSF48097 SSF48097, 1 hit
SSF54236 SSF54236, 1 hit
PROSITEiView protein in PROSITE
PS50841 DIX, 1 hit
PS50132 RGS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAXIN2_MOUSE
AccessioniPrimary (citable) accession number: O88566
Secondary accession number(s): Q6PFZ9, Q9QXJ6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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