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UniProtKB - O88552 (CLD2_MOUSE)
Protein
Claudin-2
Gene
Cldn2
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
2 PublicationsGO - Molecular functioni
- identical protein binding Source: UniProtKB
- structural molecule activity Source: InterPro
GO - Biological processi
- bicellular tight junction assembly Source: GO_Central
- calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
- cell adhesion Source: GO_Central
- cell-cell adhesion Source: UniProtKB
Protein family/group databases
TCDBi | 1.H.1.1.11, the claudin tight junction (claudin1) family |
Names & Taxonomyi
Protein namesi | Recommended name: Claudin-2 |
Gene namesi | Name:Cldn2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1276110, Cldn2 |
VEuPathDBi | HostDB:ENSMUSG00000047230 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Other locations
- tight junction 2 Publications
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- bicellular tight junction Source: UniProtKB
- cell-cell junction Source: MGI
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 81 | ExtracellularSequence analysisAdd BLAST | 53 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 116 | CytoplasmicSequence analysisAdd BLAST | 14 | |
Transmembranei | 117 – 137 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 138 – 162 | ExtracellularSequence analysisAdd BLAST | 25 | |
Transmembranei | 163 – 183 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 184 – 230 | CytoplasmicSequence analysisAdd BLAST | 47 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPathology & Biotechi
Disruption phenotypei
Both female and male Cldn2-knockout mice are similarly hypercalciuric compared to wild-type littermates. Cldn2-null mice are hypercalciuric due to a primary defect in renal tubule calcium transport and develop papillary nephrocalcinosis. Cldn2-null mice are also found to have increased net intestinal calcium absorption, but reduced paracellular calcium permeability in the colon, suggesting reduced intestinal calcium secretion.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144735 | 1 – 230 | Claudin-2Add BLAST | 230 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 54 ↔ 64 | By similarity | ||
Cross-linki | 218 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Modified residuei | 219 | PhosphoserineCombined sources | 1 | |
Modified residuei | 223 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
The disulfide bond is necessary for pore formation, but is not required for correct protein trafficking.By similarity
Keywords - PTMi
Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
MaxQBi | O88552 |
PaxDbi | O88552 |
PeptideAtlasi | O88552 |
PRIDEi | O88552 |
ProteomicsDBi | 285489 |
PTM databases
iPTMneti | O88552 |
PhosphoSitePlusi | O88552 |
Expressioni
Tissue specificityi
Expressed in the kidney, liver and intestine, with higher levels in the ileum than in the jejunum. Low levels in the brain.2 Publications
Inductioni
By CDX1 and CDX2. Induction by CDX2, but not CDX1, is potentiated by TCF1.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000047230, Expressed in adult mammalian kidney and 160 other tissues |
ExpressionAtlasi | O88552, baseline and differential |
Genevisiblei | O88552, MM |
Interactioni
Subunit structurei
Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN1, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.
2 PublicationsGO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
MINTi | O88552 |
STRINGi | 10090.ENSMUSP00000054219 |
Miscellaneous databases
RNActi | O88552, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | O88552 |
SMRi | O88552 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 205 – 230 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 229 – 230 | Interactions with TJP1, TJP2 and TJP3By similarity | 2 |
Sequence similaritiesi
Belongs to the claudin family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502R10A, Eukaryota |
GeneTreei | ENSGT00940000160785 |
HOGENOMi | CLU_076370_1_2_1 |
InParanoidi | O88552 |
OMAi | VWNIHVV |
OrthoDBi | 1209440at2759 |
PhylomeDBi | O88552 |
TreeFami | TF331936 |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR005411, Claudin2 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01589, CLAUDIN2 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O88552-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASLGVQLVG YILGLLGLLG TSIAMLLPNW RTSSYVGASI VTAVGFSKGL
60 70 80 90 100
WMECATHSTG ITQCDIYSTL LGLPADIQAA QAMMVTSSAM SSLACIISVV
110 120 130 140 150
GMRCTVFCQD SRAKDRVAVV GGVFFILGGI LGFIPVAWNL HGILRDFYSP
160 170 180 190 200
LVPDSMKFEI GEALYLGIIS ALFSLVAGVI LCFSCSPQGN RTNYYDGYQA
210 220 230
QPLATRSSPR SAQQPKAKSE FNSYSLTGYV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA3KGB5 | A3KGB5_MOUSE | Claudin | Cldn2 | 131 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF072128 mRNA Translation: AAC27079.1 AK004990 mRNA Translation: BAB23725.1 BC015252 mRNA Translation: AAH15252.1 BC085494 mRNA Translation: AAH85494.1 |
CCDSi | CCDS30437.1 |
RefSeqi | NP_057884.1, NM_016675.4 XP_006528553.1, XM_006528490.3 |
Genome annotation databases
Ensembli | ENSMUST00000054889; ENSMUSP00000054219; ENSMUSG00000047230 |
GeneIDi | 12738 |
KEGGi | mmu:12738 |
UCSCi | uc009ukl.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF072128 mRNA Translation: AAC27079.1 AK004990 mRNA Translation: BAB23725.1 BC015252 mRNA Translation: AAH15252.1 BC085494 mRNA Translation: AAH85494.1 |
CCDSi | CCDS30437.1 |
RefSeqi | NP_057884.1, NM_016675.4 XP_006528553.1, XM_006528490.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4P5H | X-ray | 3.38 | 1/2/3/4/P/Q/R/S/T/U/V/W/X/Y/Z | 141-160 | [»] | |
AlphaFoldDBi | O88552 | |||||
SMRi | O88552 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
MINTi | O88552 |
STRINGi | 10090.ENSMUSP00000054219 |
Protein family/group databases
TCDBi | 1.H.1.1.11, the claudin tight junction (claudin1) family |
PTM databases
iPTMneti | O88552 |
PhosphoSitePlusi | O88552 |
Proteomic databases
MaxQBi | O88552 |
PaxDbi | O88552 |
PeptideAtlasi | O88552 |
PRIDEi | O88552 |
ProteomicsDBi | 285489 |
Protocols and materials databases
Antibodypediai | 3617, 585 antibodies from 33 providers |
DNASUi | 12738 |
Genome annotation databases
Ensembli | ENSMUST00000054889; ENSMUSP00000054219; ENSMUSG00000047230 |
GeneIDi | 12738 |
KEGGi | mmu:12738 |
UCSCi | uc009ukl.2, mouse |
Organism-specific databases
CTDi | 9075 |
MGIi | MGI:1276110, Cldn2 |
VEuPathDBi | HostDB:ENSMUSG00000047230 |
Phylogenomic databases
eggNOGi | ENOG502R10A, Eukaryota |
GeneTreei | ENSGT00940000160785 |
HOGENOMi | CLU_076370_1_2_1 |
InParanoidi | O88552 |
OMAi | VWNIHVV |
OrthoDBi | 1209440at2759 |
PhylomeDBi | O88552 |
TreeFami | TF331936 |
Miscellaneous databases
BioGRID-ORCSi | 12738, 3 hits in 74 CRISPR screens |
ChiTaRSi | Cldn2, mouse |
PROi | PR:O88552 |
RNActi | O88552, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000047230, Expressed in adult mammalian kidney and 160 other tissues |
ExpressionAtlasi | O88552, baseline and differential |
Genevisiblei | O88552, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR005411, Claudin2 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01589, CLAUDIN2 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CLD2_MOUSE | |
Accessioni | O88552Primary (citable) accession number: O88552 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families