UniProtKB - O88551 (CLD1_MOUSE)
Protein
Claudin-1
Gene
Cldn1
Organism
Mus musculus (Mouse)
Status
Functioni
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.3 Publications
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- structural molecule activity Source: InterPro
- virus receptor activity Source: MGI
GO - Biological processi
- aging Source: Ensembl
- bicellular tight junction assembly Source: MGI
- calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
- cell adhesion Source: GO_Central
- cell-cell junction organization Source: MGI
- cell junction maintenance Source: ARUK-UCL
- cellular response to butyrate Source: Ensembl
- cellular response to interferon-gamma Source: Ensembl
- cellular response to lead ion Source: Ensembl
- cellular response to transforming growth factor beta stimulus Source: Ensembl
- cellular response to tumor necrosis factor Source: Ensembl
- establishment of blood-nerve barrier Source: MGI
- establishment of endothelial intestinal barrier Source: MGI
- establishment of skin barrier Source: UniProtKB
- hyperosmotic salinity response Source: Ensembl
- liver regeneration Source: Ensembl
- positive regulation of bicellular tight junction assembly Source: MGI
- positive regulation of cell migration Source: MGI
- positive regulation of epithelial cell proliferation involved in wound healing Source: MGI
- positive regulation of wound healing Source: MGI
- protein complex oligomerization Source: MGI
- response to dexamethasone Source: Ensembl
- response to ethanol Source: Ensembl
- response to interleukin-18 Source: MGI
- response to lipopolysaccharide Source: Ensembl
- response to toxic substance Source: Ensembl
- xenobiotic transport across blood-nerve barrier Source: MGI
Names & Taxonomyi
Protein namesi | Recommended name: Claudin-1 |
Gene namesi | Name:Cldn1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1276109, Cldn1 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
- Basolateral cell membrane By similarity
Other locations
- tight junction 3 Publications
Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.By similarity
Plasma Membrane
- apical plasma membrane Source: MGI
- basolateral plasma membrane Source: MGI
- integral component of plasma membrane Source: UniProtKB
- lateral plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- bicellular tight junction Source: UniProtKB
- cell junction Source: CACAO
- cell-cell junction Source: ARUK-UCL
- cytoplasm Source: MGI
- integral component of membrane Source: UniProtKB
- protein-containing complex Source: MGI
- tight junction Source: ARUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicBy similarity | 7 | |
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 81 | ExtracellularSequence analysisAdd BLAST | 53 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 115 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 116 – 136 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 137 – 163 | ExtracellularSequence analysisAdd BLAST | 27 | |
Transmembranei | 164 – 184 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 185 – 211 | CytoplasmicBy similarityAdd BLAST | 27 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPathology & Biotechi
Disruption phenotypei
Complete perinatal lethality. Mice are born at the expected Mendelian rate, but die within one day after birth, due to severe defects in the skin barrier function, leading to rapid transepidermal water loss and dehydration.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144730 | 1 – 211 | Claudin-1Add BLAST | 211 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 54 ↔ 64 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | O88551 |
PaxDbi | O88551 |
PRIDEi | O88551 |
ProteomicsDBi | 285488 |
PTM databases
iPTMneti | O88551 |
PhosphoSitePlusi | O88551 |
Expressioni
Tissue specificityi
Detected in epidermis and liver (at protein level). Widely expressed, with highest levels in liver and kidney.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000022512, Expressed in liver and 247 other tissues |
ExpressionAtlasi | O88551, baseline and differential |
Genevisiblei | O88551, MM |
Interactioni
Subunit structurei
Can form homo- and heteropolymers with other CLDN. Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.
Interacts with MPDZ and PATJ.
Interacts with OCLN, CD81, CLDN4, CLDN6 and CLDN9 (By similarity).
By similarity5 PublicationsBinary interactionsi
O88551
With | #Exp. | IntAct |
---|---|---|
Mpdz [Q8VBX6] | 2 | EBI-7158428,EBI-8026435 |
GO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 198743, 2 interactors |
IntActi | O88551, 2 interactors |
MINTi | O88551 |
STRINGi | 10090.ENSMUSP00000023154 |
Miscellaneous databases
RNActi | O88551, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 210 – 211 | Interactions with TJP1, TJP2, TJP3 and PATJ | 2 |
Sequence similaritiesi
Belongs to the claudin family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502R7HF, Eukaryota |
GeneTreei | ENSGT00940000155387 |
HOGENOMi | CLU_076370_2_3_1 |
InParanoidi | O88551 |
OMAi | IGMKCMK |
OrthoDBi | 1244077at2759 |
PhylomeDBi | O88551 |
TreeFami | TF331936 |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003548, Claudin1 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF92, PTHR12002:SF92, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01377, CLAUDIN1 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
i Sequence
Sequence statusi: Complete.
O88551-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANAGLQLLG FILASLGWIG SIVSTALPQW KIYSYAGDNI VTAQAIYEGL
60 70 80 90 100
WMSCVSQSTG QIQCKVFDSL LNLNSTLQAT RALMVIGILL GLIAIFVSTI
110 120 130 140 150
GMKCMRCLED DEVQKMWMAV IGGIIFLISG LATLVATAWY GNRIVQEFYD
160 170 180 190 200
PLTPINARYE FGQALFTGWA AASLCLLGGV LLSCSCPRKT TSYPTPRPYP
210
KPTPSSGKDY V
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF072127 mRNA Translation: AAC27078.1 AK028428 mRNA Translation: BAC25945.1 AK036762 mRNA Translation: BAC29567.1 AK036780 mRNA Translation: BAC29574.1 AK040604 mRNA Translation: BAC30640.1 AK054207 mRNA Translation: BAC35693.1 AK081601 mRNA Translation: BAC38267.1 BC002003 mRNA Translation: AAH02003.1 |
CCDSi | CCDS28087.1 |
RefSeqi | NP_057883.1, NM_016674.4 |
Genome annotation databases
Ensembli | ENSMUST00000023154; ENSMUSP00000023154; ENSMUSG00000022512 |
GeneIDi | 12737 |
KEGGi | mmu:12737 |
UCSCi | uc007yuz.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF072127 mRNA Translation: AAC27078.1 AK028428 mRNA Translation: BAC25945.1 AK036762 mRNA Translation: BAC29567.1 AK036780 mRNA Translation: BAC29574.1 AK040604 mRNA Translation: BAC30640.1 AK054207 mRNA Translation: BAC35693.1 AK081601 mRNA Translation: BAC38267.1 BC002003 mRNA Translation: AAH02003.1 |
CCDSi | CCDS28087.1 |
RefSeqi | NP_057883.1, NM_016674.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 198743, 2 interactors |
IntActi | O88551, 2 interactors |
MINTi | O88551 |
STRINGi | 10090.ENSMUSP00000023154 |
PTM databases
iPTMneti | O88551 |
PhosphoSitePlusi | O88551 |
Proteomic databases
jPOSTi | O88551 |
PaxDbi | O88551 |
PRIDEi | O88551 |
ProteomicsDBi | 285488 |
Protocols and materials databases
Antibodypediai | 3613, 774 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000023154; ENSMUSP00000023154; ENSMUSG00000022512 |
GeneIDi | 12737 |
KEGGi | mmu:12737 |
UCSCi | uc007yuz.2, mouse |
Organism-specific databases
CTDi | 9076 |
MGIi | MGI:1276109, Cldn1 |
Phylogenomic databases
eggNOGi | ENOG502R7HF, Eukaryota |
GeneTreei | ENSGT00940000155387 |
HOGENOMi | CLU_076370_2_3_1 |
InParanoidi | O88551 |
OMAi | IGMKCMK |
OrthoDBi | 1244077at2759 |
PhylomeDBi | O88551 |
TreeFami | TF331936 |
Miscellaneous databases
BioGRID-ORCSi | 12737, 0 hits in 52 CRISPR screens |
ChiTaRSi | Cldn1, mouse |
PROi | PR:O88551 |
RNActi | O88551, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022512, Expressed in liver and 247 other tissues |
ExpressionAtlasi | O88551, baseline and differential |
Genevisiblei | O88551, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003548, Claudin1 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF92, PTHR12002:SF92, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01377, CLAUDIN1 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLD1_MOUSE | |
Accessioni | O88551Primary (citable) accession number: O88551 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | November 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families