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Protein

Palmitoyl-protein thioesterase 1

Gene

Ppt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes thioester-linked fatty acyl groups such as palmitate from modified cysteine residues in proteins or peptides during lysosomal degradation. Prefers acyl chain lengths of 14 to 18 carbons.By similarity

Catalytic activityi

Palmitoyl-[protein] + H2O = palmitate + [protein].By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei115By similarity1
Active sitei233By similarity1
Active sitei289By similarity1

GO - Molecular functioni

  • palmitoyl-(protein) hydrolase activity Source: MGI
  • palmitoyl-CoA hydrolase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

ReactomeiR-MMU-75105 Fatty acyl-CoA biosynthesis

Protein family/group databases

ESTHERimouse-ppt Palmitoyl-protein_thioesterase

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyl-protein thioesterase 1 (EC:3.1.2.22)
Short name:
PPT-1
Alternative name(s):
Palmitoyl-protein hydrolase 1
Gene namesi
Name:Ppt1
Synonyms:Cln1, Ppt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1298204 Ppt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27By similarityAdd BLAST27
ChainiPRO_000002555228 – 306Palmitoyl-protein thioesterase 1Add BLAST279

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi45 ↔ 46By similarity
Disulfide bondi96 ↔ 128By similarity
Disulfide bondi152 ↔ 160By similarity
Glycosylationi197N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi212N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi232N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO88531
PaxDbiO88531
PeptideAtlasiO88531
PRIDEiO88531

PTM databases

iPTMnetiO88531
PhosphoSitePlusiO88531
SwissPalmiO88531

Expressioni

Tissue specificityi

Highest level in testis and kidney, lower in heart, brain and lung and lowest in skeletal muscle.2 Publications

Developmental stagei

Expressed in the retina at a higher level than that in the brain at all developmental stages and this high level of expression in the retina is detectable much earlier than that in the brain (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000028657 Expressed in 306 organ(s), highest expression level in brainstem
CleanExiMM_PPT1
ExpressionAtlasiO88531 baseline and differential
GenevisibleiO88531 MM

Interactioni

Subunit structurei

Interacts with CLN5, ATP5F1A and ATP5F1B (PubMed:19941651).1 Publication

Protein-protein interaction databases

IntActiO88531, 2 interactors
STRINGi10090.ENSMUSP00000030412

Structurei

3D structure databases

ProteinModelPortaliO88531
SMRiO88531
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2541 Eukaryota
COG1075 LUCA
GeneTreeiENSGT00530000063368
HOVERGENiHBG018186
InParanoidiO88531
KOiK01074
OMAiGFFIHPN
OrthoDBiEOG091G0HJH
TreeFamiTF323926

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002472 Palm_thioest
IPR030294 PPT1
PANTHERiPTHR11247:SF8 PTHR11247:SF8, 1 hit
PfamiView protein in Pfam
PF02089 Palm_thioest, 1 hit
PRINTSiPR00414 PPTHIESTRASE
SUPFAMiSSF53474 SSF53474, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O88531-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASSCSRRLL AAALLPWCCA AWALGHLDPP SPPPLVIWHG MGDSCCNPMS
60 70 80 90 100
MGVIKKMVEK EIPGIYVLSL EIGKNMMEDV ENSFFLNVNV QVNMVCQILE
110 120 130 140 150
KDPKLQQGYN AIGFSQGGQF LRAVAQRCPT PPMMTLISVG GQHQGVFGLP
160 170 180 190 200
RCPGESSHIC DFIRKSLNAG AYSKLVQERL VQAQYWHDPI KESVYRNYSI
210 220 230 240 250
FLADINQERC VNESYKKNLM ALKKFVMVKF FNDSIVDPVD SEWFGFYRSG
260 270 280 290 300
QAKETIPLQE STLYTEDRLG LKKMDKAGKL VFLAKEGDHL QISKEWFTAH

IIPFLK
Length:306
Mass (Da):34,490
Last modified:March 5, 2002 - v2
Checksum:iCC7D9B65ECAF46A8
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1B0P8B1B0P8_MOUSE
Palmitoyl-protein thioesterase 1
Ppt1
179Annotation score:
B1B0P9B1B0P9_MOUSE
Palmitoyl-protein thioesterase 1
Ppt1
197Annotation score:
F7CTA8F7CTA8_MOUSE
Palmitoyl-protein thioesterase 1
Ppt1
54Annotation score:

Sequence cautioni

The sequence AAC25398 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11A → R in AAD25224 (PubMed:10231585).Curated1
Sequence conflicti18C → S in AAD25224 (PubMed:10231585).Curated1
Sequence conflicti109Missing in AAD25224 (PubMed:10231585).Curated1
Sequence conflicti118G → GY in AAD25224 (PubMed:10231585).Curated1
Sequence conflicti151R → L in AK014561 (PubMed:16141072).Curated1
Sequence conflicti255 – 257TIP → NIA in AAD25224 (PubMed:10231585).Curated3
Sequence conflicti276K → R in AAD25224 (PubMed:10231585).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071025 mRNA Translation: AAC25398.1 Different initiation.
AF087568 mRNA Translation: AAD25224.1
AK014561 mRNA No translation available.
CCDSiCCDS38870.1
RefSeqiNP_032943.2, NM_008917.3
UniGeneiMm.277719

Genome annotation databases

EnsembliENSMUST00000030412; ENSMUSP00000030412; ENSMUSG00000028657
GeneIDi19063
KEGGimmu:19063
UCSCiuc008uoh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071025 mRNA Translation: AAC25398.1 Different initiation.
AF087568 mRNA Translation: AAD25224.1
AK014561 mRNA No translation available.
CCDSiCCDS38870.1
RefSeqiNP_032943.2, NM_008917.3
UniGeneiMm.277719

3D structure databases

ProteinModelPortaliO88531
SMRiO88531
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88531, 2 interactors
STRINGi10090.ENSMUSP00000030412

Protein family/group databases

ESTHERimouse-ppt Palmitoyl-protein_thioesterase

PTM databases

iPTMnetiO88531
PhosphoSitePlusiO88531
SwissPalmiO88531

Proteomic databases

EPDiO88531
PaxDbiO88531
PeptideAtlasiO88531
PRIDEiO88531

Protocols and materials databases

DNASUi19063
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030412; ENSMUSP00000030412; ENSMUSG00000028657
GeneIDi19063
KEGGimmu:19063
UCSCiuc008uoh.2 mouse

Organism-specific databases

CTDi5538
MGIiMGI:1298204 Ppt1

Phylogenomic databases

eggNOGiKOG2541 Eukaryota
COG1075 LUCA
GeneTreeiENSGT00530000063368
HOVERGENiHBG018186
InParanoidiO88531
KOiK01074
OMAiGFFIHPN
OrthoDBiEOG091G0HJH
TreeFamiTF323926

Enzyme and pathway databases

ReactomeiR-MMU-75105 Fatty acyl-CoA biosynthesis

Miscellaneous databases

ChiTaRSiPpt1 mouse
PROiPR:O88531
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028657 Expressed in 306 organ(s), highest expression level in brainstem
CleanExiMM_PPT1
ExpressionAtlasiO88531 baseline and differential
GenevisibleiO88531 MM

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR002472 Palm_thioest
IPR030294 PPT1
PANTHERiPTHR11247:SF8 PTHR11247:SF8, 1 hit
PfamiView protein in Pfam
PF02089 Palm_thioest, 1 hit
PRINTSiPR00414 PPTHIESTRASE
SUPFAMiSSF53474 SSF53474, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPPT1_MOUSE
AccessioniPrimary (citable) accession number: O88531
Secondary accession number(s): Q9D681, Q9WTY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 5, 2002
Last modified: September 12, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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