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UniProtKB - O88509 (DNM3B_MOUSE)

Entry version 178 (22 Apr 2020)
Sequence version 2 (01 May 2000)
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Protein

DNA (cytosine-5)-methyltransferase 3B

Gene

Dnmt3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Functions as a transcriptional corepressor by associating with ZHX1 (By similarity). Required for DUX4 silencing in somatic cells (By similarity).By similarity6 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Activated by binding to the regulatory factor DNMT3L.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei611S-adenosyl-L-methionineBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei657PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri439 – 469GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST31
Zinc fingeri480 – 536PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST57

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionActivator, DNA-binding, Methyltransferase, Repressor, Transferase
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.1.1.37 3474

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-212300 PRC2 methylates histones and DNA
R-MMU-4655427 SUMOylation of DNA methylation proteins

Protein family/group databases

Restriction enzymes and methylases database

More...REBASEi		3748 M.MmuDnmt3B

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA (cytosine-5)-methyltransferase 3B (EC:2.1.1.37)
Short name:
Dnmt3b
Alternative name(s):
DNA methyltransferase MmuIIIB
Short name:
DNA MTase MmuIIIB
Short name:
M.MmuIIIB
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Dnmt3b
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 2

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:1261819 Dnmt3b

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi236 – 237VW → RR: Prevents accumulation in pericentric heterochromatin. 1 Publication2
Mutagenesisi277S → P: Prevents accumulation in pericentric heterochromatin. 1 Publication1
Mutagenesisi609A → T: Significantly reduces activity. 1 Publication1
Mutagenesisi656 – 657PC → GT: No effect on localization. 1 Publication2
Mutagenesisi669G → S: Significantly reduces activity. 1 Publication1
Mutagenesisi670L → T: Significantly reduces activity. 1 Publication1
Mutagenesisi725V → G: Loss of activity. 1 Publication1
Mutagenesisi823D → G: Significantly reduces activity. 1 Publication1
Mutagenesisi824V → M: Significantly reduces activity. 1 Publication1

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL2189115

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000880461 – 859DNA (cytosine-5)-methyltransferase 3BAdd BLAST859

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei96PhosphoserineBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki102Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei112PhosphothreonineBy similarity1
Modified residuei116PhosphoserineBy similarity1
Modified residuei216PhosphoserineBy similarity1
Modified residuei415Citrulline1 Publication1
Cross-linki623Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Sumoylated.By similarity
Citrullinated by PADI4.1 Publication

Keywords - PTMi

Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		O88509

MaxQB - The MaxQuant DataBase

More...MaxQBi		O88509

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O88509

PeptideAtlas

More...PeptideAtlasi		O88509

PRoteomics IDEntifications database

More...PRIDEi		O88509

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		O88509

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		O88509

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Expressed in almost all blastocysts at 3.0 dpc. Preferentially expressed in the trophectoderm (TE) in 3.5 dpc and polar TE in 4.0 dpc blastocysts. In 4.5 dpc embryos, expressed in the polar TE and some inner cell mass (ICM) embryonic lineage cells. In post-implantation embryo at 5.5 dpc, expressed in the epiblast (embryonic lineage) derived from the ICM. Highly expressed, at 7.5 dpc, in the embryonic ectoderm, neural ectoderm, and chorionic ectoderm; a weak expression is also detected in mesodermal and endodermal cells. At later stages, the expression is detected predominantly in the forebrain and eyes but weakly throughout the embryo.2 Publications

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000027478 Expressed in epiblast (generic) and 277 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		O88509 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		O88509 MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with CBX4, DNMT1, DNMT3A, SETDB1, UBE2I9, UBL1 and ZHX1 (By similarity).

Interacts with SUV39H1 and BAZ2A/TIP5.

Interacts with the PRC2/EED-EZH2 complex.

Interacts with UHRF1.

By similarity6 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Show more details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...BioGridi		199262, 11 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		O88509

Database of interacting proteins

More...DIPi		DIP-43736N

Protein interaction database and analysis system

More...IntActi		O88509, 11 interactors

Molecular INTeraction database

More...MINTi		O88509

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000105396

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		O88509

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		O88509 protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1859
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O88509

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		O88509

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini232 – 290PWWPPROSITE-ProRule annotationAdd BLAST59
Domaini428 – 560ADDPROSITE-ProRule annotationAdd BLAST133
Domaini581 – 859SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST279

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 305Interaction with DNMT1 and DNMT3ABy similarityAdd BLAST305
Regioni440 – 532Interaction with the PRC2/EED-EZH2 complexAdd BLAST93
Regioni588 – 592S-adenosyl-L-methionine bindingBy similarity5
Regioni633 – 635S-adenosyl-L-methionine bindingBy similarity3
Regioni838 – 840S-adenosyl-L-methionine bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi160 – 165Poly-Arg6

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The PWWP domain is essential for targeting to pericentric heterochromatin.

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri439 – 469GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST31
Zinc fingeri480 – 536PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST57

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		ENOG410IGHW Eukaryota
ENOG410XQ4Y LUCA

Ensembl GeneTree

More...GeneTreei		ENSGT00940000156928

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_006958_9_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		O88509

KEGG Orthology (KO)

More...KOi		K17399

Identification of Orthologs from Complete Genome Data

More...OMAi		IKPMLDW

Database of Orthologous Groups

More...OrthoDBi		1015783at2759

TreeFam database of animal gene trees

More...TreeFami		TF329039

Family and domain databases

Conserved Domains Database

More...CDDi		cd11728 ADDz_Dnmt3b, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR025766 ADD
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR040552 DNMT3_ADD
IPR030488 DNMT3B_ADD
IPR000313 PWWP_dom
IPR029063 SAM-dependent_MTases
IPR011011 Znf_FYVE_PHD

Pfam protein domain database

More...Pfami		View protein in Pfam
PF17980 ADD_DNMT3, 1 hit
PF00145 DNA_methylase, 1 hit
PF00855 PWWP, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00293 PWWP, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF53335 SSF53335, 1 hit
SSF57903 SSF57903, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS51533 ADD, 1 hit
PS00094 C5_MTASE_1, 1 hit
PS50812 PWWP, 1 hit
PS51679 SAM_MT_C5, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O88509-1) [UniParc]FASTAAdd to basket
Also known as: 5

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKGDSRHLNE EEGASGYEEC IIVNGNFSDQ SSDTKDAPSP PVLEAICTEP 
        60         70         80         90        100
VCTPETRGRR SSSRLSKREV SSLLNYTQDM TGDGDRDDEV DDGNGSDILM 
       110        120        130        140        150
PKLTRETKDT RTRSESPAVR TRHSNGTSSL ERQRASPRIT RGRQGRHHVQ 
       160        170        180        190        200
EYPVEFPATR SRRRRASSSA STPWSSPASV DFMEEVTPKS VSTPSVDLSQ 
       210        220        230        240        250
DGDQEGMDTT QVDAESRDGD STEYQDDKEF GIGDLVWGKI KGFSWWPAMV 
       260        270        280        290        300
VSWKATSKRQ AMPGMRWVQW FGDGKFSEIS ADKLVALGLF SQHFNLATFN 
       310        320        330        340        350
KLVSYRKAMY HTLEKARVRA GKTFSSSPGE SLEDQLKPML EWAHGGFKPT 
       360        370        380        390        400
GIEGLKPNKK QPVVNKSKVR RSDSRNLEPR RRENKSRRRT TNDSAASESP 
       410        420        430        440        450
PPKRLKTNSY GGKDRGEDEE SRERMASEVT NNKGNLEDRC LSCGKKNPVS 
       460        470        480        490        500
FHPLFEGGLC QSCRDRFLEL FYMYDEDGYQ SYCTVCCEGR ELLLCSNTSC 
       510        520        530        540        550
CRCFCVECLE VLVGAGTAED AKLQEPWSCY MCLPQRCHGV LRRRKDWNMR 
       560        570        580        590        600
LQDFFTTDPD LEEFEPPKLY PAIPAAKRRP IRVLSLFDGI ATGYLVLKEL 
       610        620        630        640        650
GIKVEKYIAS EVCAESIAVG TVKHEGQIKY VNDVRKITKK NIEEWGPFDL 
       660        670        680        690        700
VIGGSPCNDL SNVNPARKGL YEGTGRLFFE FYHLLNYTRP KEGDNRPFFW 
       710        720        730        740        750
MFENVVAMKV NDKKDISRFL ACNPVMIDAI KVSAAHRARY FWGNLPGMNR 
       760        770        780        790        800
PVMASKNDKL ELQDCLEFSR TAKLKKVQTI TTKSNSIRQG KNQLFPVVMN 
       810        820        830        840        850
GKDDVLWCTE LERIFGFPAH YTDVSNMGRG ARQKLLGRSW SVPVIRHLFA 

PLKDYFACE
Length:859
Mass (Da):97,228
Last modified:May 1, 2000 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i93E334D9FBCC590E
GO
Isoform 2 (identifier: O88509-2) [UniParc]FASTAAdd to basket
Also known as: 6

The sequence of this isoform differs from the canonical sequence as follows:
     363-382: Missing.

Show »
Length:839
Mass (Da):94,793
Checksum:i59BF48AB459A1298
GO
Isoform 3 (identifier: O88509-3) [UniParc]FASTAAdd to basket
Also known as: 8

The sequence of this isoform differs from the canonical sequence as follows:
     363-382: Missing.
     750-812: Missing.

Show »
Length:776
Mass (Da):87,586
Checksum:i211E2DE8BF06C48E
GO
Isoform 4 (identifier: O88509-4) [UniParc]FASTAAdd to basket
Also known as: 7

The sequence of this isoform differs from the canonical sequence as follows:
     750-812: Missing.

Show »
Length:796
Mass (Da):90,021
Checksum:i0B7D93B4D802744E
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3KR45Q3KR45_MOUSE
DNA (cytosine-5)-methyltransferase ...
Dnmt3b
860Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti217 – 218RD → IY in AAF74515 (PubMed:12567489).Curated2
Sequence conflicti217 – 218RD → IY in AAF74516 (PubMed:12567489).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_005642363 – 382Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST20
Alternative sequenceiVSP_005643750 – 812Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST63

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF068626 mRNA Translation: AAC40178.2
AF068627 mRNA Translation: AAC40179.2
AF068628 mRNA Translation: AAC40180.2
AF151969 mRNA Translation: AAF74515.1
AF151970 mRNA Translation: AAF74516.1
AF151971 mRNA Translation: AAF74517.1
AF151972 mRNA Translation: AAF74518.1
AF151973 mRNA Translation: AAF74519.1
AF151974 mRNA Translation: AAF74520.1
AF151975 mRNA Translation: AAF74521.1
AF151976 mRNA Translation: AAF74522.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS16913.1 [O88509-2]
CCDS16914.1 [O88509-1]
CCDS16915.1 [O88509-4]
CCDS16916.1 [O88509-3]

NCBI Reference Sequences

More...RefSeqi		NP_001003960.2, NM_001003960.4 [O88509-2]
NP_001003961.2, NM_001003961.4 [O88509-1]
NP_001258673.1, NM_001271744.1 [O88509-1]
NP_001258674.1, NM_001271745.1 [O88509-2]
NP_001258676.1, NM_001271747.1 [O88509-3]
NP_034198.3, NM_010068.5 [O88509-3]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000072997; ENSMUSP00000072761; ENSMUSG00000027478 [O88509-1]
ENSMUST00000081628; ENSMUSP00000080334; ENSMUSG00000027478 [O88509-2]
ENSMUST00000088976; ENSMUSP00000086370; ENSMUSG00000027478 [O88509-4]
ENSMUST00000103150; ENSMUSP00000099439; ENSMUSG00000027478 [O88509-3]
ENSMUST00000103151; ENSMUSP00000099440; ENSMUSG00000027478 [O88509-3]
ENSMUST00000109772; ENSMUSP00000105394; ENSMUSG00000027478 [O88509-4]
ENSMUST00000109773; ENSMUSP00000105395; ENSMUSG00000027478 [O88509-2]
ENSMUST00000109774; ENSMUSP00000105396; ENSMUSG00000027478 [O88509-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		13436

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:13436

UCSC genome browser

More...UCSCi		uc008nib.3 mouse [O88509-1]
uc008nic.3 mouse [O88509-2]
uc008nie.3 mouse [O88509-3]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068626 mRNA Translation: AAC40178.2
AF068627 mRNA Translation: AAC40179.2
AF068628 mRNA Translation: AAC40180.2
AF151969 mRNA Translation: AAF74515.1
AF151970 mRNA Translation: AAF74516.1
AF151971 mRNA Translation: AAF74517.1
AF151972 mRNA Translation: AAF74518.1
AF151973 mRNA Translation: AAF74519.1
AF151974 mRNA Translation: AAF74520.1
AF151975 mRNA Translation: AAF74521.1
AF151976 mRNA Translation: AAF74522.1
CCDSiCCDS16913.1 [O88509-2]
CCDS16914.1 [O88509-1]
CCDS16915.1 [O88509-4]
CCDS16916.1 [O88509-3]
RefSeqiNP_001003960.2, NM_001003960.4 [O88509-2]
NP_001003961.2, NM_001003961.4 [O88509-1]
NP_001258673.1, NM_001271744.1 [O88509-1]
NP_001258674.1, NM_001271745.1 [O88509-2]
NP_001258676.1, NM_001271747.1 [O88509-3]
NP_034198.3, NM_010068.5 [O88509-3]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1KHCX-ray1.80A219-362[»]
SMRiO88509
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi199262, 11 interactors
CORUMiO88509
DIPiDIP-43736N
IntActiO88509, 11 interactors
MINTiO88509
STRINGi10090.ENSMUSP00000105396

Chemistry databases

BindingDBiO88509
ChEMBLiCHEMBL2189115

Protein family/group databases

REBASEi3748 M.MmuDnmt3B

PTM databases

iPTMnetiO88509
PhosphoSitePlusiO88509

Proteomic databases

jPOSTiO88509
MaxQBiO88509
PaxDbiO88509
PeptideAtlasiO88509
PRIDEiO88509

Genome annotation databases

EnsembliENSMUST00000072997; ENSMUSP00000072761; ENSMUSG00000027478 [O88509-1]
ENSMUST00000081628; ENSMUSP00000080334; ENSMUSG00000027478 [O88509-2]
ENSMUST00000088976; ENSMUSP00000086370; ENSMUSG00000027478 [O88509-4]
ENSMUST00000103150; ENSMUSP00000099439; ENSMUSG00000027478 [O88509-3]
ENSMUST00000103151; ENSMUSP00000099440; ENSMUSG00000027478 [O88509-3]
ENSMUST00000109772; ENSMUSP00000105394; ENSMUSG00000027478 [O88509-4]
ENSMUST00000109773; ENSMUSP00000105395; ENSMUSG00000027478 [O88509-2]
ENSMUST00000109774; ENSMUSP00000105396; ENSMUSG00000027478 [O88509-1]
GeneIDi13436
KEGGimmu:13436
UCSCiuc008nib.3 mouse [O88509-1]
uc008nic.3 mouse [O88509-2]
uc008nie.3 mouse [O88509-3]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		1789
MGIiMGI:1261819 Dnmt3b

Phylogenomic databases

eggNOGiENOG410IGHW Eukaryota
ENOG410XQ4Y LUCA
GeneTreeiENSGT00940000156928
HOGENOMiCLU_006958_9_1_1
InParanoidiO88509
KOiK17399
OMAiIKPMLDW
OrthoDBi1015783at2759
TreeFamiTF329039

Enzyme and pathway databases

BRENDAi2.1.1.37 3474
ReactomeiR-MMU-212300 PRC2 methylates histones and DNA
R-MMU-4655427 SUMOylation of DNA methylation proteins

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Dnmt3b mouse
EvolutionaryTraceiO88509

Protein Ontology

More...PROi		PR:O88509
RNActiO88509 protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000027478 Expressed in epiblast (generic) and 277 other tissues
ExpressionAtlasiO88509 baseline and differential
GenevisibleiO88509 MM

Family and domain databases

CDDicd11728 ADDz_Dnmt3b, 1 hit
InterProiView protein in InterPro
IPR025766 ADD
IPR018117 C5_DNA_meth_AS
IPR001525 C5_MeTfrase
IPR040552 DNMT3_ADD
IPR030488 DNMT3B_ADD
IPR000313 PWWP_dom
IPR029063 SAM-dependent_MTases
IPR011011 Znf_FYVE_PHD
PfamiView protein in Pfam
PF17980 ADD_DNMT3, 1 hit
PF00145 DNA_methylase, 1 hit
PF00855 PWWP, 1 hit
SMARTiView protein in SMART
SM00293 PWWP, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
SSF57903 SSF57903, 1 hit
PROSITEiView protein in PROSITE
PS51533 ADD, 1 hit
PS00094 C5_MTASE_1, 1 hit
PS50812 PWWP, 1 hit
PS51679 SAM_MT_C5, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiDNM3B_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O88509
Secondary accession number(s): O88510, O88511
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 2000
Last modified: April 22, 2020
This is version 178 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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