Dnmt3b

Functionⁱ

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing. In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Function as transcriptional corepressor by associating with ZHX1 (By similarity). Required for DUX4 silencing in somatic cells (By similarity).By similarity6 Publications

Catalytic activityⁱ

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Activity regulationⁱ

Activated by binding to the regulatory factor DNMT3L.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Binding siteⁱ	611	S-adenosyl-L-methionineBy similarity		1
Active siteⁱ	657	PROSITE-ProRule annotation		1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	439 – 469	GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST		31
Zinc fingerⁱ	480 – 536	PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST		57

GO - Molecular functionⁱ

chromatin binding Source: MGI
DNA (cytosine-5-)-methyltransferase activity
Source: MGI
Inferred from direct assayⁱ
- 16543361
- 9662389
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
Source: MGI
Inferred from direct assayⁱ
- 27856912
DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates Source: Reactome
DNA binding
Source: MGI
Inferred from direct assayⁱ
- 21378119
histone deacetylase binding Source: MGI
metal ion binding Source: UniProtKB-KW
methyltransferase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11919202
transcription corepressor activity Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

C-5 methylation of cytosine
Source: MGI
Inferred from direct assayⁱ
- 27856912
cellular response to amino acid stimulus
Source: MGI
Inferred from direct assayⁱ
- 20548288
DNA methylation
Source: MGI
Inferred from mutant phenotypeⁱ
- 22133874
DNA methylation involved in embryo development
Source: MGI
Inferred from genetic interactionⁱ
- 10555141
inactivation of X chromosome by DNA methylation
Source: MGI
Inferred from mutant phenotypeⁱ
- 23754746
methylation-dependent chromatin silencing
Source: MGI
Inferred from genetic interactionⁱ
- 17938196
negative regulation of gene expression, epigenetic
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 18056424
negative regulation of histone H3-K9 methylation Source: MGI
negative regulation of transcription, DNA-templated Source: MGI
negative regulation of transcription by RNA polymerase II
Source: MGI
Inferred from mutant phenotypeⁱ
- 22133874
positive regulation of gene expression Source: MGI
positive regulation of histone H3-K4 methylation Source: MGI
positive regulation of neuron differentiation Source: MGI
protein-containing complex localization
Source: MGI
Inferred from direct assayⁱ
- 16543361
regulation of gene expression by genetic imprinting
Source: MGI
Inferred from mutant phenotypeⁱ
- 11934864

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, DNA-binding, Methyltransferase, Repressor, Transferase
Ligand	Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

BRENDAⁱ	2.1.1.37 3474
Reactomeⁱ	R-MMU-212300 PRC2 methylates histones and DNA R-MMU-4655427 SUMOylation of DNA methylation proteins

Protein family/group databases

REBASEⁱ	3748 M.MmuDnmt3B

REBASEⁱ

3748 M.MmuDnmt3B

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: DNA (cytosine-5)-methyltransferase 3B (EC:2.1.1.37) Short name: Dnmt3b Alternative name(s): DNA methyltransferase MmuIIIB Short name: DNA MTase MmuIIIB Short name: M.MmuIIIB
Gene namesⁱ	Name:Dnmt3b
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 2

Organism-specific databases

MGIⁱ	MGI:1261819 Dnmt3b

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	236 – 237	VW → RR: Prevents accumulation in pericentric heterochromatin. 1 Publication	2
Mutagenesisⁱ	277	S → P: Prevents accumulation in pericentric heterochromatin. 1 Publication	1
Mutagenesisⁱ	609	A → T: Significantly reduces activity. 1 Publication	1
Mutagenesisⁱ	656 – 657	PC → GT: No effect on localization. 1 Publication	2
Mutagenesisⁱ	669	G → S: Significantly reduces activity. 1 Publication	1
Mutagenesisⁱ	670	L → T: Significantly reduces activity. 1 Publication	1
Mutagenesisⁱ	725	V → G: Loss of activity. 1 Publication	1
Mutagenesisⁱ	823	D → G: Significantly reduces activity. 1 Publication	1
Mutagenesisⁱ	824	V → M: Significantly reduces activity. 1 Publication	1

Chemistry databases

ChEMBLⁱ	CHEMBL2189115

ChEMBLⁱ

CHEMBL2189115

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000088046	1 – 859	DNA (cytosine-5)-methyltransferase 3BAdd BLAST		859

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	96	PhosphoserineBy similarity	1
Cross-linkⁱ	102	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residueⁱ	112	PhosphothreonineBy similarity	1
Modified residueⁱ	116	PhosphoserineBy similarity	1
Modified residueⁱ	216	PhosphoserineBy similarity	1
Modified residueⁱ	415	Citrulline1 Publication	1
Cross-linkⁱ	623	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Post-translational modificationⁱ

Sumoylated.By similarity

Citrullinated by PADI4.1 Publication

Keywords - PTMⁱ

Citrullination, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O88509
PaxDbⁱ	O88509
PeptideAtlas More...PeptideAtlasⁱ	O88509
PRIDEⁱ	O88509

PTM databases

iPTMnetⁱ	O88509
PhosphoSitePlusⁱ	O88509

Expressionⁱ

Developmental stageⁱ

Expressed in almost all blastocysts at embryonic day 3.0 (E3.0). Preferentially expressed in the trophectoderm (TE) in E3.5 and polar TE in E4.0 blastocysts. In E4.5 embryos, expressed in the polar TE and some inner cell mass (ICM) embryonic lineage cells. In post-implantation embryo at E5.5, expressed in the epiblast (embryonic lineage) derived from the ICM. Highly expressed, at E7.5, in the embryonic ectoderm, neural ectoderm, and chorionic ectoderm; a weak expression is also detected in mesodermal and endodermal cells. At later stages, the expression is detected predominantly in the forebrain and eyes but weakly throughout the embryo.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000027478 Expressed in 278 organ(s), highest expression level in epiblast (generic)
CleanExⁱ	MM_DNMT3B
ExpressionAtlasⁱ	O88509 baseline and differential
Genevisibleⁱ	O88509 MM

Interactionⁱ

Subunit structureⁱ

Interacts with CBX4, DNMT1, DNMT3A, SETDB1, UBE2I9, UBL1 and ZHX1 (By similarity). Interacts with SUV39H1 and BAZ2A/TIP5. Interacts with the PRC2/EED-EZH2 complex. Interacts with UHRF1.By similarity6 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
Bmi1	P25916	2	EBI-7987547,EBI-927401
Dnmt3l	Q9CWR8	6	EBI-7987547,EBI-3043871
Ehmt2	Q9Z148-2	3	EBI-7987547,EBI-15737169
Hells	Q60848	4	EBI-7987547,EBI-3043887

GO - Molecular functionⁱ

histone deacetylase binding Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	199262, 11 interactors
CORUMⁱ	O88509
Database of interacting proteins More...DIPⁱ	DIP-43736N
IntActⁱ	O88509, 11 interactors
Molecular INTeraction database More...MINTⁱ	O88509
STRINGⁱ	10090.ENSMUSP00000051830

Chemistry databases

BindingDBⁱ

O88509

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	226 – 229	Combined sources	4
Beta strandⁱ	235 – 240	Combined sources	6
Turnⁱ	241 – 243	Combined sources	3
Beta strandⁱ	244 – 251	Combined sources	8
Helixⁱ	253 – 255	Combined sources	3
Beta strandⁱ	265 – 270	Combined sources	6
Turnⁱ	271 – 273	Combined sources	3
Beta strandⁱ	276 – 280	Combined sources	5
Helixⁱ	281 – 283	Combined sources	3
Helixⁱ	290 – 293	Combined sources	4
Helixⁱ	296 – 301	Combined sources	6
Helixⁱ	303 – 320	Combined sources	18
Helixⁱ	332 – 344	Combined sources	13
Turnⁱ	348 – 350	Combined sources	3
Helixⁱ	351 – 355	Combined sources	5

Show more details

3D structure databases

ProteinModelPortalⁱ	O88509
SMRⁱ	O88509
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	O88509

EvolutionaryTraceⁱ

O88509

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	232 – 290	PWWPPROSITE-ProRule annotationAdd BLAST	59
Domainⁱ	428 – 560	ADDPROSITE-ProRule annotationAdd BLAST	133
Domainⁱ	581 – 859	SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST	279

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 305	Interaction with DNMT1 and DNMT3ABy similarityAdd BLAST	305
Regionⁱ	440 – 532	Interaction with the PRC2/EED-EZH2 complexAdd BLAST	93
Regionⁱ	588 – 592	S-adenosyl-L-methionine bindingBy similarity	5
Regionⁱ	633 – 635	S-adenosyl-L-methionine bindingBy similarity	3
Regionⁱ	838 – 840	S-adenosyl-L-methionine bindingBy similarity	3

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	160 – 165	Poly-Arg		6

Domainⁱ

The PWWP domain is essential for targeting to pericentric heterochromatin.

Sequence similaritiesⁱ

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	439 – 469	GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST		31
Zinc fingerⁱ	480 – 536	PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST		57

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	ENOG410IGHW Eukaryota ENOG410XQ4Y LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000008341
HOGENOMⁱ	HOG000230875
HOVERGENⁱ	HBG051381
InParanoidⁱ	O88509
KEGG Orthology (KO) More...KOⁱ	K17399
OMAⁱ	MKGDTRQ
TreeFamⁱ	TF329039

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd11728 ADDz_Dnmt3b, 1 hit
InterProⁱ	View protein in InterPro IPR025766 ADD IPR018117 C5_DNA_meth_AS IPR001525 C5_MeTfrase IPR030488 DNMT3B_ADD IPR000313 PWWP_dom IPR029063 SAM-dependent_MTases IPR011011 Znf_FYVE_PHD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00145 DNA_methylase, 1 hit PF00855 PWWP, 1 hit
SMARTⁱ	View protein in SMART SM00293 PWWP, 1 hit
SUPFAMⁱ	SSF53335 SSF53335, 1 hit SSF57903 SSF57903, 1 hit
PROSITEⁱ	View protein in PROSITE PS51533 ADD, 1 hit PS00094 C5_MTASE_1, 1 hit PS50812 PWWP, 1 hit PS51679 SAM_MT_C5, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: O88509-1) [UniParc]FASTA Add to basket

Also known as: 5

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKGDSRHLNE EEGASGYEEC IIVNGNFSDQ SSDTKDAPSP PVLEAICTEP 
        60         70         80         90        100
VCTPETRGRR SSSRLSKREV SSLLNYTQDM TGDGDRDDEV DDGNGSDILM 
       110        120        130        140        150
PKLTRETKDT RTRSESPAVR TRHSNGTSSL ERQRASPRIT RGRQGRHHVQ 
       160        170        180        190        200
EYPVEFPATR SRRRRASSSA STPWSSPASV DFMEEVTPKS VSTPSVDLSQ 
       210        220        230        240        250
DGDQEGMDTT QVDAESRDGD STEYQDDKEF GIGDLVWGKI KGFSWWPAMV 
       260        270        280        290        300
VSWKATSKRQ AMPGMRWVQW FGDGKFSEIS ADKLVALGLF SQHFNLATFN 
       310        320        330        340        350
KLVSYRKAMY HTLEKARVRA GKTFSSSPGE SLEDQLKPML EWAHGGFKPT 
       360        370        380        390        400
GIEGLKPNKK QPVVNKSKVR RSDSRNLEPR RRENKSRRRT TNDSAASESP 
       410        420        430        440        450
PPKRLKTNSY GGKDRGEDEE SRERMASEVT NNKGNLEDRC LSCGKKNPVS 
       460        470        480        490        500
FHPLFEGGLC QSCRDRFLEL FYMYDEDGYQ SYCTVCCEGR ELLLCSNTSC 
       510        520        530        540        550
CRCFCVECLE VLVGAGTAED AKLQEPWSCY MCLPQRCHGV LRRRKDWNMR 
       560        570        580        590        600
LQDFFTTDPD LEEFEPPKLY PAIPAAKRRP IRVLSLFDGI ATGYLVLKEL 
       610        620        630        640        650
GIKVEKYIAS EVCAESIAVG TVKHEGQIKY VNDVRKITKK NIEEWGPFDL 
       660        670        680        690        700
VIGGSPCNDL SNVNPARKGL YEGTGRLFFE FYHLLNYTRP KEGDNRPFFW 
       710        720        730        740        750
MFENVVAMKV NDKKDISRFL ACNPVMIDAI KVSAAHRARY FWGNLPGMNR 
       760        770        780        790        800
PVMASKNDKL ELQDCLEFSR TAKLKKVQTI TTKSNSIRQG KNQLFPVVMN 
       810        820        830        840        850
GKDDVLWCTE LERIFGFPAH YTDVSNMGRG ARQKLLGRSW SVPVIRHLFA 

PLKDYFACE

Length:859

Mass (Da):97,228

Last modified:May 1, 2000 - v2

Checksum:ⁱ93E334D9FBCC590E

GO

Isoform 2 (identifier: O88509-2) [UniParc]FASTA Add to basket

Also known as: 6

The sequence of this isoform differs from the canonical sequence as follows:
363-382: Missing.

« Hide

        10         20         30         40         50
MKGDSRHLNE EEGASGYEEC IIVNGNFSDQ SSDTKDAPSP PVLEAICTEP 
        60         70         80         90        100
VCTPETRGRR SSSRLSKREV SSLLNYTQDM TGDGDRDDEV DDGNGSDILM 
       110        120        130        140        150
PKLTRETKDT RTRSESPAVR TRHSNGTSSL ERQRASPRIT RGRQGRHHVQ 
       160        170        180        190        200
EYPVEFPATR SRRRRASSSA STPWSSPASV DFMEEVTPKS VSTPSVDLSQ 
       210        220        230        240        250
DGDQEGMDTT QVDAESRDGD STEYQDDKEF GIGDLVWGKI KGFSWWPAMV 
       260        270        280        290        300
VSWKATSKRQ AMPGMRWVQW FGDGKFSEIS ADKLVALGLF SQHFNLATFN 
       310        320        330        340        350
KLVSYRKAMY HTLEKARVRA GKTFSSSPGE SLEDQLKPML EWAHGGFKPT 
       360        370        380        390        400
GIEGLKPNKK QPENKSRRRT TNDSAASESP PPKRLKTNSY GGKDRGEDEE 
       410        420        430        440        450
SRERMASEVT NNKGNLEDRC LSCGKKNPVS FHPLFEGGLC QSCRDRFLEL 
       460        470        480        490        500
FYMYDEDGYQ SYCTVCCEGR ELLLCSNTSC CRCFCVECLE VLVGAGTAED 
       510        520        530        540        550
AKLQEPWSCY MCLPQRCHGV LRRRKDWNMR LQDFFTTDPD LEEFEPPKLY 
       560        570        580        590        600
PAIPAAKRRP IRVLSLFDGI ATGYLVLKEL GIKVEKYIAS EVCAESIAVG 
       610        620        630        640        650
TVKHEGQIKY VNDVRKITKK NIEEWGPFDL VIGGSPCNDL SNVNPARKGL 
       660        670        680        690        700
YEGTGRLFFE FYHLLNYTRP KEGDNRPFFW MFENVVAMKV NDKKDISRFL 
       710        720        730        740        750
ACNPVMIDAI KVSAAHRARY FWGNLPGMNR PVMASKNDKL ELQDCLEFSR 
       760        770        780        790        800
TAKLKKVQTI TTKSNSIRQG KNQLFPVVMN GKDDVLWCTE LERIFGFPAH 
       810        820        830 
YTDVSNMGRG ARQKLLGRSW SVPVIRHLFA PLKDYFACE

Show »

Length:839

Mass (Da):94,793

Checksum:ⁱ59BF48AB459A1298

GO

Isoform 3 (identifier: O88509-3) [UniParc]FASTA Add to basket

Also known as: 8

The sequence of this isoform differs from the canonical sequence as follows:
363-382: Missing.
750-812: Missing.

« Hide

        10         20         30         40         50
MKGDSRHLNE EEGASGYEEC IIVNGNFSDQ SSDTKDAPSP PVLEAICTEP 
        60         70         80         90        100
VCTPETRGRR SSSRLSKREV SSLLNYTQDM TGDGDRDDEV DDGNGSDILM 
       110        120        130        140        150
PKLTRETKDT RTRSESPAVR TRHSNGTSSL ERQRASPRIT RGRQGRHHVQ 
       160        170        180        190        200
EYPVEFPATR SRRRRASSSA STPWSSPASV DFMEEVTPKS VSTPSVDLSQ 
       210        220        230        240        250
DGDQEGMDTT QVDAESRDGD STEYQDDKEF GIGDLVWGKI KGFSWWPAMV 
       260        270        280        290        300
VSWKATSKRQ AMPGMRWVQW FGDGKFSEIS ADKLVALGLF SQHFNLATFN 
       310        320        330        340        350
KLVSYRKAMY HTLEKARVRA GKTFSSSPGE SLEDQLKPML EWAHGGFKPT 
       360        370        380        390        400
GIEGLKPNKK QPENKSRRRT TNDSAASESP PPKRLKTNSY GGKDRGEDEE 
       410        420        430        440        450
SRERMASEVT NNKGNLEDRC LSCGKKNPVS FHPLFEGGLC QSCRDRFLEL 
       460        470        480        490        500
FYMYDEDGYQ SYCTVCCEGR ELLLCSNTSC CRCFCVECLE VLVGAGTAED 
       510        520        530        540        550
AKLQEPWSCY MCLPQRCHGV LRRRKDWNMR LQDFFTTDPD LEEFEPPKLY 
       560        570        580        590        600
PAIPAAKRRP IRVLSLFDGI ATGYLVLKEL GIKVEKYIAS EVCAESIAVG 
       610        620        630        640        650
TVKHEGQIKY VNDVRKITKK NIEEWGPFDL VIGGSPCNDL SNVNPARKGL 
       660        670        680        690        700
YEGTGRLFFE FYHLLNYTRP KEGDNRPFFW MFENVVAMKV NDKKDISRFL 
       710        720        730        740        750
ACNPVMIDAI KVSAAHRARY FWGNLPGMNR IFGFPAHYTD VSNMGRGARQ 
       760        770 
KLLGRSWSVP VIRHLFAPLK DYFACE

Show »

Length:776

Mass (Da):87,586

Checksum:ⁱ211E2DE8BF06C48E

GO

Isoform 4 (identifier: O88509-4) [UniParc]FASTA Add to basket

Also known as: 7

The sequence of this isoform differs from the canonical sequence as follows:
750-812: Missing.

« Hide

        10         20         30         40         50
MKGDSRHLNE EEGASGYEEC IIVNGNFSDQ SSDTKDAPSP PVLEAICTEP 
        60         70         80         90        100
VCTPETRGRR SSSRLSKREV SSLLNYTQDM TGDGDRDDEV DDGNGSDILM 
       110        120        130        140        150
PKLTRETKDT RTRSESPAVR TRHSNGTSSL ERQRASPRIT RGRQGRHHVQ 
       160        170        180        190        200
EYPVEFPATR SRRRRASSSA STPWSSPASV DFMEEVTPKS VSTPSVDLSQ 
       210        220        230        240        250
DGDQEGMDTT QVDAESRDGD STEYQDDKEF GIGDLVWGKI KGFSWWPAMV 
       260        270        280        290        300
VSWKATSKRQ AMPGMRWVQW FGDGKFSEIS ADKLVALGLF SQHFNLATFN 
       310        320        330        340        350
KLVSYRKAMY HTLEKARVRA GKTFSSSPGE SLEDQLKPML EWAHGGFKPT 
       360        370        380        390        400
GIEGLKPNKK QPVVNKSKVR RSDSRNLEPR RRENKSRRRT TNDSAASESP 
       410        420        430        440        450
PPKRLKTNSY GGKDRGEDEE SRERMASEVT NNKGNLEDRC LSCGKKNPVS 
       460        470        480        490        500
FHPLFEGGLC QSCRDRFLEL FYMYDEDGYQ SYCTVCCEGR ELLLCSNTSC 
       510        520        530        540        550
CRCFCVECLE VLVGAGTAED AKLQEPWSCY MCLPQRCHGV LRRRKDWNMR 
       560        570        580        590        600
LQDFFTTDPD LEEFEPPKLY PAIPAAKRRP IRVLSLFDGI ATGYLVLKEL 
       610        620        630        640        650
GIKVEKYIAS EVCAESIAVG TVKHEGQIKY VNDVRKITKK NIEEWGPFDL 
       660        670        680        690        700
VIGGSPCNDL SNVNPARKGL YEGTGRLFFE FYHLLNYTRP KEGDNRPFFW 
       710        720        730        740        750
MFENVVAMKV NDKKDISRFL ACNPVMIDAI KVSAAHRARY FWGNLPGMNR 
       760        770        780        790 
IFGFPAHYTD VSNMGRGARQ KLLGRSWSVP VIRHLFAPLK DYFACE

Show »

Length:796

Mass (Da):90,021

Checksum:ⁱ0B7D93B4D802744E

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	Q3KR45	Q3KR45_MOUSE		DNA (cytosine-5)-methyltransferase ... DNA (cytosine-5)-methyltransferase 3B (DNA methyltransferase 3B)	Dnmt3b	860	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	217 – 218	RD → IY in AAF74515 (PubMed:12567489).Curated		2
Sequence conflictⁱ	217 – 218	RD → IY in AAF74516 (PubMed:12567489).Curated		2

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_005642	363 – 382	Missing in isoform 2 and isoform 3. 2 PublicationsAdd BLAST		20
Alternative sequenceⁱVSP_005643	750 – 812	Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST		63

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF068626 mRNA Translation: AAC40178.2 AF068627 mRNA Translation: AAC40179.2 AF068628 mRNA Translation: AAC40180.2 AF151969 mRNA Translation: AAF74515.1 AF151970 mRNA Translation: AAF74516.1 AF151971 mRNA Translation: AAF74517.1 AF151972 mRNA Translation: AAF74518.1 AF151973 mRNA Translation: AAF74519.1 AF151974 mRNA Translation: AAF74520.1 AF151975 mRNA Translation: AAF74521.1 AF151976 mRNA Translation: AAF74522.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS16913.1 [O88509-2] CCDS16914.1 [O88509-1] CCDS16915.1 [O88509-4] CCDS16916.1 [O88509-3]
NCBI Reference Sequences More...RefSeqⁱ	NP_001003960.2, NM_001003960.4 [O88509-2] NP_001003961.2, NM_001003961.4 [O88509-1] NP_001258673.1, NM_001271744.1 [O88509-1] NP_001258674.1, NM_001271745.1 [O88509-2] NP_001258676.1, NM_001271747.1 [O88509-3] NP_034198.3, NM_010068.5 [O88509-3]
UniGeneⁱ	Mm.491804 Mm.89772

Genome annotation databases

Ensemblⁱ	ENSMUST00000072997; ENSMUSP00000072761; ENSMUSG00000027478 [O88509-1] ENSMUST00000081628; ENSMUSP00000080334; ENSMUSG00000027478 [O88509-2] ENSMUST00000088976; ENSMUSP00000086370; ENSMUSG00000027478 [O88509-4] ENSMUST00000103150; ENSMUSP00000099439; ENSMUSG00000027478 [O88509-3] ENSMUST00000103151; ENSMUSP00000099440; ENSMUSG00000027478 [O88509-3] ENSMUST00000109772; ENSMUSP00000105394; ENSMUSG00000027478 [O88509-4] ENSMUST00000109773; ENSMUSP00000105395; ENSMUSG00000027478 [O88509-2] ENSMUST00000109774; ENSMUSP00000105396; ENSMUSG00000027478 [O88509-1]
GeneIDⁱ	13436
KEGGⁱ	mmu:13436
UCSC genome browser More...UCSCⁱ	uc008nib.3 mouse [O88509-1] uc008nic.3 mouse [O88509-2] uc008nie.3 mouse [O88509-3]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O88509-4	DNA cytosine methyltransferase 3b6		MOUSE		796	UniRef100_Q541E5
UPI0003D73CE6		MOUSE		808

Protein	Similar proteins		Species	Score	Length	Source
O88509	DNA (cytosine-5)-methyltransferase 3B		MOUSE		860	UniRef90_O88509
DNA methyltransferase 3B (Fragment)		MERUN		67
DNA methyltransferase 3 beta		RAT		859
UPI00022F484D		CRIGR		866
UPI000A31515A		MUSCR		861
+9
O88509-2	Isoform 3 of DNA (cytosine-5)-methyltransferase 3B		MOUSE		776	UniRef90_O88509-2
DNA methyltransferase 3 beta		RAT		731
UPI00038C3AAA		MICOH		839
UPI0003D74F5E		MOUSE		851
UPI0003D71D38		MOUSE		852
+2
O88509-3	Isoform 2 of DNA (cytosine-5)-methyltransferase 3B		MOUSE		839	UniRef90_O88509-2
DNA methyltransferase 3 beta		RAT		731
UPI00038C3AAA		MICOH		839
UPI0003D74F5E		MOUSE		851
UPI0003D71D38		MOUSE		852
+2

Protein	Similar proteins		Species	Score	Length	Source
O88509	DNA (cytosine-5)-methyltransferase 3B	)	HUMAN		853	UniRef50_Q9UBC3
DNA methyltransferase 3 beta		PANTR		853
Uncharacterized protein		PANPA		853
DNA (Cytosine-5)-methyltransferase 3B isoform X1		URSMA		889
DNMT3B		CEREH		884
+711

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF068626 mRNA Translation: AAC40178.2 AF068627 mRNA Translation: AAC40179.2 AF068628 mRNA Translation: AAC40180.2 AF151969 mRNA Translation: AAF74515.1 AF151970 mRNA Translation: AAF74516.1 AF151971 mRNA Translation: AAF74517.1 AF151972 mRNA Translation: AAF74518.1 AF151973 mRNA Translation: AAF74519.1 AF151974 mRNA Translation: AAF74520.1 AF151975 mRNA Translation: AAF74521.1 AF151976 mRNA Translation: AAF74522.1
CCDSⁱ	CCDS16913.1 [O88509-2] CCDS16914.1 [O88509-1] CCDS16915.1 [O88509-4] CCDS16916.1 [O88509-3]
RefSeqⁱ	NP_001003960.2, NM_001003960.4 [O88509-2] NP_001003961.2, NM_001003961.4 [O88509-1] NP_001258673.1, NM_001271744.1 [O88509-1] NP_001258674.1, NM_001271745.1 [O88509-2] NP_001258676.1, NM_001271747.1 [O88509-3] NP_034198.3, NM_010068.5 [O88509-3]
UniGeneⁱ	Mm.491804 Mm.89772

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1KHC	X-ray	1.80	A	219-362	[»]
ProteinModelPortalⁱ	O88509
SMRⁱ	O88509
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	199262, 11 interactors
CORUMⁱ	O88509
DIPⁱ	DIP-43736N
IntActⁱ	O88509, 11 interactors
MINTⁱ	O88509
STRINGⁱ	10090.ENSMUSP00000051830

Chemistry databases

BindingDBⁱ	O88509
ChEMBLⁱ	CHEMBL2189115

Protein family/group databases

REBASEⁱ	3748 M.MmuDnmt3B

REBASEⁱ

3748 M.MmuDnmt3B

PTM databases

iPTMnetⁱ	O88509
PhosphoSitePlusⁱ	O88509

Proteomic databases

MaxQBⁱ	O88509
PaxDbⁱ	O88509
PeptideAtlasⁱ	O88509
PRIDEⁱ	O88509

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Organism-specific databases

CTDⁱ	1789
MGIⁱ	MGI:1261819 Dnmt3b

Phylogenomic databases

eggNOGⁱ	ENOG410IGHW Eukaryota ENOG410XQ4Y LUCA
GeneTreeⁱ	ENSGT00390000008341
HOGENOMⁱ	HOG000230875
HOVERGENⁱ	HBG051381
InParanoidⁱ	O88509
KOⁱ	K17399
OMAⁱ	MKGDTRQ
TreeFamⁱ	TF329039

Enzyme and pathway databases

BRENDAⁱ	2.1.1.37 3474
Reactomeⁱ	R-MMU-212300 PRC2 methylates histones and DNA R-MMU-4655427 SUMOylation of DNA methylation proteins

Miscellaneous databases

ChiTaRSⁱ	Dnmt3b mouse
EvolutionaryTraceⁱ	O88509
Protein Ontology More...PROⁱ	PR:O88509
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000027478 Expressed in 278 organ(s), highest expression level in epiblast (generic)
CleanExⁱ	MM_DNMT3B
ExpressionAtlasⁱ	O88509 baseline and differential
Genevisibleⁱ	O88509 MM

Family and domain databases

CDDⁱ	cd11728 ADDz_Dnmt3b, 1 hit
InterProⁱ	View protein in InterPro IPR025766 ADD IPR018117 C5_DNA_meth_AS IPR001525 C5_MeTfrase IPR030488 DNMT3B_ADD IPR000313 PWWP_dom IPR029063 SAM-dependent_MTases IPR011011 Znf_FYVE_PHD
Pfamⁱ	View protein in Pfam PF00145 DNA_methylase, 1 hit PF00855 PWWP, 1 hit
SMARTⁱ	View protein in SMART SM00293 PWWP, 1 hit
SUPFAMⁱ	SSF53335 SSF53335, 1 hit SSF57903 SSF57903, 1 hit
PROSITEⁱ	View protein in PROSITE PS51533 ADD, 1 hit PS00094 C5_MTASE_1, 1 hit PS50812 PWWP, 1 hit PS51679 SAM_MT_C5, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DNM3B_MOUSE
Accessionⁱ	O88509Primary (citable) accession number: O88509 Secondary accession number(s): O88510, O88511
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
	Last sequence update:	May 1, 2000
	Last modified:	November 7, 2018
	This is version 167 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - O88509 (DNM3B_MOUSE)

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DNA (cytosine-5)-methyltransferase 3B

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

Enzyme and pathway databases

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<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

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Keywords - PTMi

Proteomic databases

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<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

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Protein-protein interaction databases

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<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

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<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

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Experimental Info

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Keywords - Coding sequence diversityi

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<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ