UniProtKB - O88508 (DNM3A_MOUSE)
DNA (cytosine-5)-methyltransferase 3A
Dnmt3a
Functioni
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development (PubMed:9662389, PubMed:11399089, PubMed:10555141, PubMed:11919202, PubMed:16567415, PubMed:17713477).
DNA methylation is coordinated with methylation of histones (PubMed:9662389, PubMed:11399089, PubMed:10555141, PubMed:11919202, PubMed:16567415, PubMed:17713477).
It modifies DNA in a non-processive manner and also methylates non-CpG sites (PubMed:9662389, PubMed:11399089, PubMed:10555141, PubMed:11919202, PubMed:16567415, PubMed:17713477).
May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1 (PubMed:18823905).
Plays a role in paternal and maternal imprinting (PubMed:15215868).
Required for methylation of most imprinted loci in germ cells (PubMed:15215868).
Acts as a transcriptional corepressor for ZBTB18 (PubMed:11350943).
Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites (PubMed:20547484).
Can actively repress transcription through the recruitment of HDAC activity (PubMed:11350943).
Also has weak auto-methylation activity on Cys-706 in absence of DNA (PubMed:21481189).
11 PublicationsCatalytic activityi
- a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-methyl-2'-deoxycytidine in DNA + H+ + S-adenosyl-L-homocysteinePROSITE-ProRule annotation2 PublicationsEC:2.1.1.37PROSITE-ProRule annotation2 PublicationsThis reaction proceeds in the forward2 Publications direction.
- L-cysteinyl-[protein] + S-adenosyl-L-methionine = H+ + S-adenosyl-L-homocysteine + S-methyl-L-cysteinyl-[protein]1 PublicationThis reaction proceeds in the forward1 Publication direction.
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 660 | S-adenosyl-L-methionineBy similarity | 1 | |
Active sitei | 706 | PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 489 – 519 | GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 530 – 586 | PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST | 57 |
GO - Molecular functioni
- chromatin binding Source: MGI
- DNA (cytosine-5-)-methyltransferase activity Source: MGI
- DNA (cytosine-5-)-methyltransferase activity, acting on CpN substrates Source: Reactome
- DNA binding Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- methyltransferase activity Source: MGI
- protein-cysteine methyltransferase activity Source: UniProtKB
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
GO - Biological processi
- aging Source: Ensembl
- C-5 methylation of cytosine Source: MGI
- cellular response to amino acid stimulus Source: MGI
- cellular response to bisphenol A Source: MGI
- cellular response to ethanol Source: Ensembl
- cellular response to hypoxia Source: Ensembl
- DNA methylation Source: MGI
- DNA methylation-dependent heterochromatin assembly Source: MGI
- DNA methylation involved in embryo development Source: UniProtKB
- DNA methylation involved in gamete generation Source: UniProtKB
- DNA methylation on cytosine Source: MGI
- hepatocyte apoptotic process Source: Ensembl
- hypermethylation of CpG island Source: BHF-UCL
- maintenance of DNA methylation Source: BHF-UCL
- mitotic cell cycle Source: Ensembl
- negative regulation of transcription, DNA-templated Source: GO_Central
- negative regulation of transcription by RNA polymerase II Source: MGI
- neuron differentiation Source: Ensembl
- positive regulation of cell death Source: MGI
- regulation of gene expression by genomic imprinting Source: BHF-UCL
- response to cocaine Source: Ensembl
- response to estradiol Source: Ensembl
- response to ionizing radiation Source: Ensembl
- response to lead ion Source: Ensembl
- response to toxic substance Source: Ensembl
- response to vitamin A Source: Ensembl
- response to xenobiotic stimulus Source: Ensembl
- spermatogenesis Source: MGI
Keywordsi
Molecular function | Chromatin regulator, DNA-binding, Methyltransferase, Repressor, Transferase |
Biological process | Transcription, Transcription regulation |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Enzyme and pathway databases
BRENDAi | 2.1.1.37, 3474 |
Reactomei | R-MMU-212300, PRC2 methylates histones and DNA R-MMU-3214858, RMTs methylate histone arginines |
Protein family/group databases
REBASEi | 3747, M.MmuDnmt3A |
Names & Taxonomyi
Protein namesi | |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1261827, Dnmt3a |
VEuPathDBi | HostDB:ENSMUSG00000020661 |
Subcellular locationi
Nucleus
- Nucleus 3 Publications
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- Chromosome 1 Publication
Note: Accumulates in the major satellite repeats at pericentric heterochromatin.1 Publication
Nucleus
- nuclear matrix Source: UniProtKB
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- catalytic complex Source: MGI
- chromosome, centromeric region Source: UniProtKB
- cytoplasm Source: MGI
- euchromatin Source: UniProtKB
- heterochromatin Source: MGI
- XY body Source: MGI
Keywords - Cellular componenti
Chromosome, Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 293 | Missing : Decreased protein abundance. 1 Publication | 1 | |
Mutagenesisi | 302 – 303 | WP → ST: Prevents accumulation in pericentric heterochromatin. 1 Publication | 2 | |
Mutagenesisi | 306 | I → N: Decreased protein abundance. 1 Publication | 1 | |
Mutagenesisi | 326 | W → R: The protein is stably expressed. 1 Publication | 1 | |
Mutagenesisi | 636 | F → A: Reduces activity about 20-fold. Loss of substrate binding. 1 Publication | 1 | |
Mutagenesisi | 660 | E → A: Reduces activity about 15-fold. Loss of substrate binding. 1 Publication | 1 | |
Mutagenesisi | 682 | D → A: Strongly reduces substrate binding. No effect on activity. 1 Publication | 1 | |
Mutagenesisi | 705 – 706 | PC → VD: No effect on localization. 1 Publication | 2 | |
Mutagenesisi | 706 | C → A: Reduces activity about 5-fold. Reduces DNA-binding capacity. Abolished cysteine-methylation. 2 Publications | 1 | |
Mutagenesisi | 707 | N → Q: Reduces activity about 3-fold. 1 Publication | 1 | |
Mutagenesisi | 710 | S → A: No effect on activity. 1 Publication | 1 | |
Mutagenesisi | 716 | R → A: Reduces activity about 30-fold. Reduces DNA-binding capacity. 1 Publication | 1 | |
Mutagenesisi | 717 | K → A: Reduces activity about 3-fold. 1 Publication | 1 | |
Mutagenesisi | 728 | F → A: Loss of activity due to the incapacity to bind the regulatory subunit DNMT3L. 1 Publication | 1 | |
Mutagenesisi | 752 | E → A: Reduces activity about 10-fold. 1 Publication | 1 | |
Mutagenesisi | 753 | N → A: Reduces activity about 10-fold. 1 Publication | 1 | |
Mutagenesisi | 788 | R → A: Reduces activity about 15-fold. 1 Publication | 1 | |
Mutagenesisi | 827 | R → A: Reduces activity about 2-fold. Reduces DNA-binding capacity. 1 Publication | 1 | |
Mutagenesisi | 832 | R → A: Reduces DNA-binding capacity. No effect on activity. 1 Publication | 1 | |
Mutagenesisi | 878 | R → A: Reduces activity about 6-fold. Reduces DNA-binding capacity. 1 Publication | 1 | |
Mutagenesisi | 881 | R → A: Loss of activity. Strongly reduces substrate binding. 1 Publication | 1 | |
Mutagenesisi | 883 | R → A: Reduces activity about 3-fold. Reduces DNA-binding capacity. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3108652 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088044 | 1 – 908 | DNA (cytosine-5)-methyltransferase 3AAdd BLAST | 908 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 102 | PhosphoserineCombined sources | 1 | |
Modified residuei | 120 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 158 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 167 | Omega-N-methylarginineCombined sources | 1 | |
Modified residuei | 239 | PhosphoserineBy similarity | 1 | |
Modified residuei | 251 | PhosphoserineBy similarity | 1 | |
Modified residuei | 257 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 386 | PhosphoserineCombined sources | 1 | |
Modified residuei | 389 | PhosphoserineCombined sources | 1 | |
Modified residuei | 706 | S-methylcysteine; by autocatalysis1 Publication | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O88508 |
jPOSTi | O88508 |
MaxQBi | O88508 |
PaxDbi | O88508 |
PeptideAtlasi | O88508 |
PRIDEi | O88508 |
ProteomicsDBi | 277363 [O88508-1] 277364 [O88508-2] |
PTM databases
iPTMneti | O88508 |
PhosphoSitePlusi | O88508 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000020661, Expressed in embryonic post-anal tail and 393 other tissues |
ExpressionAtlasi | O88508, baseline and differential |
Genevisiblei | O88508, MM |
Interactioni
Subunit structurei
Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L) (By similarity).
Interacts with DNMT1 and DNMT3B (By similarity).
Interacts with MPHOSPH8 (By similarity).
Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4) (By similarity). Binds the ZBTB18 transcriptional repressor (PubMed:11350943).
Interacts with SETDB1 (By similarity). Associates with HDAC1 through its ADD domain (PubMed:11350943, PubMed:12616525).
Interacts with UHRF1 (PubMed:19798101).
Interacts with the PRC2/EED-EZH2 complex (PubMed:16357870).
Interacts with UBC9, PIAS1 and PIAS2 (PubMed:14752048).
Interacts with SPOCD1 (PubMed:32674113).
Interacts with ZNF263; recruited to the SIX3 promoter along with other proteins involved in chromatin modification and transcriptional corepression where it contributes to transcriptional repression (By similarity).
By similarity6 PublicationsBinary interactionsi
O88508
With | #Exp. | IntAct |
---|---|---|
Dnmt3l [Q9CWR8] | 6 | EBI-995154,EBI-3043871 |
Hells [Q60848] | 4 | EBI-995154,EBI-3043887 |
MECP2 - isoform A [P51608-1] from Homo sapiens. | 10 | EBI-995154,EBI-26687319 |
Isoform 1 [O88508-1]
With | #Exp. | IntAct |
---|---|---|
Dnmt3l [Q9CWR8] | 6 | EBI-15650457,EBI-3043871 |
Ehmt2 - isoform 2 [Q9Z148-2] | 3 | EBI-15650457,EBI-15737169 |
GO - Molecular functioni
- identical protein binding Source: MGI
- RNA polymerase II-specific DNA-binding transcription factor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 199261, 24 interactors |
CORUMi | O88508 |
DIPi | DIP-38005N |
IntActi | O88508, 15 interactors |
MINTi | O88508 |
STRINGi | 10090.ENSMUSP00000020991 |
Chemistry databases
BindingDBi | O88508 |
Miscellaneous databases
RNActi | O88508, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 288 – 346 | PWWPPROSITE-ProRule annotationAdd BLAST | 59 | |
Domaini | 478 – 610 | ADDPROSITE-ProRule annotationAdd BLAST | 133 | |
Domaini | 630 – 908 | SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST | 279 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 183 | DisorderedSequence analysisAdd BLAST | 183 | |
Regioni | 195 – 399 | Interaction with DNMT1 and DNMT3BBy similarityAdd BLAST | 205 | |
Regioni | 226 – 281 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 443 – 462 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 490 – 582 | Interaction with the PRC2/EED-EZH2 complex1 PublicationAdd BLAST | 93 | |
Regioni | 637 – 641 | S-adenosyl-L-methionine bindingBy similarity | 5 | |
Regioni | 682 – 684 | S-adenosyl-L-methionine bindingBy similarity | 3 | |
Regioni | 887 – 889 | S-adenosyl-L-methionine bindingBy similarity | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 12 – 41 | Basic and acidic residuesSequence analysisAdd BLAST | 30 | |
Compositional biasi | 63 – 80 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 226 – 240 | Polar residuesSequence analysisAdd BLAST | 15 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 489 – 519 | GATA-type; atypicalPROSITE-ProRule annotationAdd BLAST | 31 | |
Zinc fingeri | 530 – 586 | PHD-type; atypicalPROSITE-ProRule annotationAdd BLAST | 57 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG502QR6U, Eukaryota |
GeneTreei | ENSGT00940000155459 |
HOGENOMi | CLU_006958_9_1_1 |
InParanoidi | O88508 |
OMAi | DGKFSVX |
OrthoDBi | 1015783at2759 |
PhylomeDBi | O88508 |
TreeFami | TF329039 |
Family and domain databases
CDDi | cd11729, ADDz_Dnmt3a, 1 hit |
Gene3Di | 3.40.50.150, 2 hits |
IDEALi | IID50163 |
InterProi | View protein in InterPro IPR025766, ADD IPR044108, ADD_DNMT3A IPR018117, C5_DNA_meth_AS IPR001525, C5_MeTfrase IPR040552, DNMT3_ADD IPR030487, DNMT3A IPR000313, PWWP_dom IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR23068:SF10, PTHR23068:SF10, 1 hit |
Pfami | View protein in Pfam PF17980, ADD_DNMT3, 1 hit PF00145, DNA_methylase, 1 hit PF00855, PWWP, 1 hit |
SMARTi | View protein in SMART SM00293, PWWP, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51533, ADD, 1 hit PS00094, C5_MTASE_1, 1 hit PS50812, PWWP, 1 hit PS51679, SAM_MT_C5, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative promoter usage. AlignAdd to basketThis entry has 2 described isoforms and 11 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPSSGPGDTS SSSLEREDDR KEGEEQEENR GKEERQEPSA TARKVGRPGR
60 70 80 90 100
KRKHPPVESS DTPKDPAVTT KSQPMAQDSG PSDLLPNGDL EKRSEPQPEE
110 120 130 140 150
GSPAAGQKGG APAEGEGTET PPEASRAVEN GCCVTKEGRG ASAGEGKEQK
160 170 180 190 200
QTNIESMKME GSRGRLRGGL GWESSLRQRP MPRLTFQAGD PYYISKRKRD
210 220 230 240 250
EWLARWKREA EKKAKVIAVM NAVEENQASG ESQKVEEASP PAVQQPTDPA
260 270 280 290 300
SPTVATTPEP VGGDAGDKNA TKAADDEPEY EDGRGFGIGE LVWGKLRGFS
310 320 330 340 350
WWPGRIVSWW MTGRSRAAEG TRWVMWFGDG KFSVVCVEKL MPLSSFCSAF
360 370 380 390 400
HQATYNKQPM YRKAIYEVLQ VASSRAGKLF PACHDSDESD SGKAVEVQNK
410 420 430 440 450
QMIEWALGGF QPSGPKGLEP PEEEKNPYKE VYTDMWVEPE AAAYAPPPPA
460 470 480 490 500
KKPRKSTTEK PKVKEIIDER TRERLVYEVR QKCRNIEDIC ISCGSLNVTL
510 520 530 540 550
EHPLFIGGMC QNCKNCFLEC AYQYDDDGYQ SYCTICCGGR EVLMCGNNNC
560 570 580 590 600
CRCFCVECVD LLVGPGAAQA AIKEDPWNCY MCGHKGTYGL LRRREDWPSR
610 620 630 640 650
LQMFFANNHD QEFDPPKVYP PVPAEKRKPI RVLSLFDGIA TGLLVLKDLG
660 670 680 690 700
IQVDRYIASE VCEDSITVGM VRHQGKIMYV GDVRSVTQKH IQEWGPFDLV
710 720 730 740 750
IGGSPCNDLS IVNPARKGLY EGTGRLFFEF YRLLHDARPK EGDDRPFFWL
760 770 780 790 800
FENVVAMGVS DKRDISRFLE SNPVMIDAKE VSAAHRARYF WGNLPGMNRP
810 820 830 840 850
LASTVNDKLE LQECLEHGRI AKFSKVRTIT TRSNSIKQGK DQHFPVFMNE
860 870 880 890 900
KEDILWCTEM ERVFGFPVHY TDVSNMSRLA RQRLLGRSWS VPVIRHLFAP
LKEYFACV
Computationally mapped potential isoform sequencesi
There are 11 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG3UZK0 | G3UZK0_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 181 | Annotation score: | ||
G3UXY2 | G3UXY2_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 332 | Annotation score: | ||
G3UZF8 | G3UZF8_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 140 | Annotation score: | ||
G3UZN5 | G3UZN5_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 184 | Annotation score: | ||
G3UX07 | G3UX07_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 193 | Annotation score: | ||
G3UZG8 | G3UZG8_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 107 | Annotation score: | ||
G3UZY8 | G3UZY8_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 34 | Annotation score: | ||
G3UX63 | G3UX63_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 94 | Annotation score: | ||
G3UY43 | G3UY43_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 72 | Annotation score: | ||
G3UZU0 | G3UZU0_MOUSE | DNA (cytosine-5)-methyltransferase ... | Dnmt3a | 41 | Annotation score: | ||
There is more potential isoformShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 151 | Q → P in AAH07466 (PubMed:12138111).Curated | 1 | |
Sequence conflicti | 775 | M → T in BAB28644 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 781 | V → G in BAB28644 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 791 | W → R in BAB28644 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 809 | L → P in BAB28644 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 904 | Y → I in BAB28644 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_009423 | 1 – 219 | Missing in isoform 2. 2 PublicationsAdd BLAST | 219 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF068625 mRNA Translation: AAC40177.2 AF480164 mRNA Translation: AAN40038.1 AK013096 mRNA Translation: BAB28644.2 Different initiation. AK090132 mRNA Translation: BAC41110.1 AK147263 mRNA Translation: BAE27806.1 AK147627 mRNA Translation: BAE28033.1 AK147642 mRNA Translation: BAE28043.1 AK147676 mRNA Translation: BAE28067.1 AK157792 mRNA Translation: BAE34200.1 BC007466 mRNA Translation: AAH07466.1 |
CCDSi | CCDS25784.1 [O88508-2] CCDS36397.1 [O88508-1] |
RefSeqi | NP_001258682.1, NM_001271753.1 [O88508-1] NP_031898.1, NM_007872.4 [O88508-1] NP_714965.1, NM_153743.4 [O88508-2] XP_006515016.1, XM_006514953.3 [O88508-1] XP_006515019.1, XM_006514956.3 [O88508-1] |
Genome annotation databases
Ensembli | ENSMUST00000020991; ENSMUSP00000020991; ENSMUSG00000020661 [O88508-1] ENSMUST00000111186; ENSMUSP00000106817; ENSMUSG00000020661 [O88508-2] ENSMUST00000172689; ENSMUSP00000133543; ENSMUSG00000020661 [O88508-2] ENSMUST00000174817; ENSMUSP00000134009; ENSMUSG00000020661 [O88508-1] |
GeneIDi | 13435 |
KEGGi | mmu:13435 |
UCSCi | uc007mxb.1, mouse [O88508-1] |
Keywords - Coding sequence diversityi
Alternative promoter usageSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF068625 mRNA Translation: AAC40177.2 AF480164 mRNA Translation: AAN40038.1 AK013096 mRNA Translation: BAB28644.2 Different initiation. AK090132 mRNA Translation: BAC41110.1 AK147263 mRNA Translation: BAE27806.1 AK147627 mRNA Translation: BAE28033.1 AK147642 mRNA Translation: BAE28043.1 AK147676 mRNA Translation: BAE28067.1 AK157792 mRNA Translation: BAE34200.1 BC007466 mRNA Translation: AAH07466.1 |
CCDSi | CCDS25784.1 [O88508-2] CCDS36397.1 [O88508-1] |
RefSeqi | NP_001258682.1, NM_001271753.1 [O88508-1] NP_031898.1, NM_007872.4 [O88508-1] NP_714965.1, NM_153743.4 [O88508-2] XP_006515016.1, XM_006514953.3 [O88508-1] XP_006515019.1, XM_006514956.3 [O88508-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3SW9 | X-ray | 3.05 | P/Q | 39-50 | [»] | |
3SWC | X-ray | 2.33 | P/Q | 39-50 | [»] | |
AlphaFoldDBi | O88508 | |||||
SMRi | O88508 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 199261, 24 interactors |
CORUMi | O88508 |
DIPi | DIP-38005N |
IntActi | O88508, 15 interactors |
MINTi | O88508 |
STRINGi | 10090.ENSMUSP00000020991 |
Chemistry databases
BindingDBi | O88508 |
ChEMBLi | CHEMBL3108652 |
Protein family/group databases
REBASEi | 3747, M.MmuDnmt3A |
PTM databases
iPTMneti | O88508 |
PhosphoSitePlusi | O88508 |
Proteomic databases
EPDi | O88508 |
jPOSTi | O88508 |
MaxQBi | O88508 |
PaxDbi | O88508 |
PeptideAtlasi | O88508 |
PRIDEi | O88508 |
ProteomicsDBi | 277363 [O88508-1] 277364 [O88508-2] |
Protocols and materials databases
Antibodypediai | 4006, 994 antibodies from 46 providers |
DNASUi | 13435 |
Genome annotation databases
Ensembli | ENSMUST00000020991; ENSMUSP00000020991; ENSMUSG00000020661 [O88508-1] ENSMUST00000111186; ENSMUSP00000106817; ENSMUSG00000020661 [O88508-2] ENSMUST00000172689; ENSMUSP00000133543; ENSMUSG00000020661 [O88508-2] ENSMUST00000174817; ENSMUSP00000134009; ENSMUSG00000020661 [O88508-1] |
GeneIDi | 13435 |
KEGGi | mmu:13435 |
UCSCi | uc007mxb.1, mouse [O88508-1] |
Organism-specific databases
CTDi | 1788 |
MGIi | MGI:1261827, Dnmt3a |
VEuPathDBi | HostDB:ENSMUSG00000020661 |
Phylogenomic databases
eggNOGi | ENOG502QR6U, Eukaryota |
GeneTreei | ENSGT00940000155459 |
HOGENOMi | CLU_006958_9_1_1 |
InParanoidi | O88508 |
OMAi | DGKFSVX |
OrthoDBi | 1015783at2759 |
PhylomeDBi | O88508 |
TreeFami | TF329039 |
Enzyme and pathway databases
BRENDAi | 2.1.1.37, 3474 |
Reactomei | R-MMU-212300, PRC2 methylates histones and DNA R-MMU-3214858, RMTs methylate histone arginines |
Miscellaneous databases
BioGRID-ORCSi | 13435, 2 hits in 75 CRISPR screens |
ChiTaRSi | Dnmt3a, mouse |
PROi | PR:O88508 |
RNActi | O88508, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020661, Expressed in embryonic post-anal tail and 393 other tissues |
ExpressionAtlasi | O88508, baseline and differential |
Genevisiblei | O88508, MM |
Family and domain databases
CDDi | cd11729, ADDz_Dnmt3a, 1 hit |
Gene3Di | 3.40.50.150, 2 hits |
IDEALi | IID50163 |
InterProi | View protein in InterPro IPR025766, ADD IPR044108, ADD_DNMT3A IPR018117, C5_DNA_meth_AS IPR001525, C5_MeTfrase IPR040552, DNMT3_ADD IPR030487, DNMT3A IPR000313, PWWP_dom IPR029063, SAM-dependent_MTases |
PANTHERi | PTHR23068:SF10, PTHR23068:SF10, 1 hit |
Pfami | View protein in Pfam PF17980, ADD_DNMT3, 1 hit PF00145, DNA_methylase, 1 hit PF00855, PWWP, 1 hit |
SMARTi | View protein in SMART SM00293, PWWP, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS51533, ADD, 1 hit PS00094, C5_MTASE_1, 1 hit PS50812, PWWP, 1 hit PS51679, SAM_MT_C5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DNM3A_MOUSE | |
Accessioni | O88508Primary (citable) accession number: O88508 Secondary accession number(s): Q3TZK8 Q9CSE1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 26, 2001 |
Last sequence update: | May 1, 2000 | |
Last modified: | May 25, 2022 | |
This is version 202 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families