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Protein

Cytoplasmic dynein 1 intermediate chain 2

Gene

Dync1i2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes (By similarity).By similarity

GO - Molecular functioni

  • dynein heavy chain binding Source: GO_Central
  • dynein light chain binding Source: GO_Central
  • motor activity Source: UniProtKB-KW
  • protein-containing complex binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionMotor protein
Biological processTransport

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2132295 MHC class II antigen presentation
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-MMU-68877 Mitotic Prometaphase
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic dynein 1 intermediate chain 2
Alternative name(s):
Cytoplasmic dynein intermediate chain 2
Dynein intermediate chain 2, cytosolic
Short name:
DH IC-2
Gene namesi
Name:Dync1i2
Synonyms:Dnci2, Dncic2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:107750 Dync1i2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001146562 – 612Cytoplasmic dynein 1 intermediate chain 2Add BLAST611

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei51PhosphoserineBy similarity1
Modified residuei84PhosphoserineBy similarity1
Modified residuei89PhosphothreonineCombined sources1
Modified residuei91PhosphoserineCombined sources1
Modified residuei95PhosphoserineCombined sources1
Modified residuei98PhosphoserineCombined sources1

Post-translational modificationi

The phosphorylation status of Ser-84 appears to be involved in dynactin-dependent target binding.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO88487
MaxQBiO88487
PaxDbiO88487
PRIDEiO88487

2D gel databases

REPRODUCTION-2DPAGEiO88487

PTM databases

iPTMnetiO88487
PhosphoSitePlusiO88487

Expressioni

Gene expression databases

BgeeiENSMUSG00000027012 Expressed in 315 organ(s), highest expression level in brain
CleanExiMM_DYNC1I2
ExpressionAtlasiO88487 baseline and differential
GenevisibleiO88487 MM

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with BICD2 (PubMed:22956769). Interacts with SPEF2 (PubMed:28619825).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199256, 9 interactors
IntActiO88487, 12 interactors
MINTiO88487
STRINGi10090.ENSMUSP00000097605

Structurei

3D structure databases

ProteinModelPortaliO88487
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati251 – 300WD 1Add BLAST50
Repeati304 – 344WD 2Add BLAST41
Repeati353 – 394WD 3Add BLAST42
Repeati403 – 443WD 4Add BLAST41
Repeati448 – 493WD 5Add BLAST46
Repeati496 – 536WD 6Add BLAST41
Repeati542 – 581WD 7Add BLAST40

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1587 Eukaryota
ENOG410XQ99 LUCA
GeneTreeiENSGT00730000110228
HOVERGENiHBG004083
InParanoidiO88487
KOiK10415
OMAiTIFPCHR
TreeFamiTF300553

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR025956 DYNC1I1/DYNC1I2
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF11540 Dynein_IC2, 1 hit
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50294 WD_REPEATS_REGION, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O88487-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKETDQK KEAAVSVQEE
60 70 80 90 100
SDLEKKRREA EALLQSMGLT TDSPIVPPPM SPSSKSVSTP SEAGSQDSGD
110 120 130 140 150
GAVGSRRGPI KLGMAKITQV DFPPREIVTY TKETQTPVTA QPKEDEEEED
160 170 180 190 200
DVATPKPPVE PEEEKTLKKD EENDSKAPPH ELTEEEKQQI LHSEEFLSFF
210 220 230 240 250
DHSTRIVERA LSEQINIFFD YSGRDLEDKE GEIQAGAKLS LNRQFFDERW
260 270 280 290 300
SKHRVVSCLD WSSQYPELLV ASYNNNEEAP HEPDGVALVW NMKYKKTTPE
310 320 330 340 350
YVFHCQSAVM SATFAKFHPN LVVGGTYSGQ IVLWDNRSNK RTPVQRTPLS
360 370 380 390 400
AAAHTHPVYC VNVVGTQNAH NLISISTDGK ICSWSLDMLS HPQDSMELVH
410 420 430 440 450
KQSKAVAVTS MSFPVGDVNN FVVGSEEGSV YTACRHGSKA GISEMFEGHQ
460 470 480 490 500
GPITGIHCHA AVGAVDFSHL FVTSSFDWTV KLWTTKNNKP LYSFEDNSDY
510 520 530 540 550
VYDVMWSPTH PALFACVDGM GRLDLWNLNN DTEVPTASIS VEGNPALNRV
560 570 580 590 600
RWTHSGREIA VGDSEGQIVI YDVGEQIAVP RNDEWARFGR TLAEINANRA
610
DAEEEAATRI PA
Length:612
Mass (Da):68,394
Last modified:November 1, 1998 - v1
Checksum:i1D14CEECF62A5E33
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2BFF9A2BFF9_MOUSE
Cytoplasmic dynein 1 intermediate c...
Dync1i2
638Annotation score:
A2BFF5A2BFF5_MOUSE
Cytoplasmic dynein 1 intermediate c...
Dync1i2
637Annotation score:
A2BFF8A2BFF8_MOUSE
Cytoplasmic dynein 1 intermediate c...
Dync1i2
611Annotation score:
Q3TPJ8Q3TPJ8_MOUSE
Cytoplasmic dynein 1 intermediate c...
Dync1i2 mCG_19348
632Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063231 mRNA Translation: AAC33446.1
AK088015 mRNA Translation: BAC40097.1
AK164437 mRNA Translation: BAE37788.1
AK168730 mRNA Translation: BAE40571.1
CCDSiCCDS16112.1
RefSeqiNP_001185805.1, NM_001198876.1
NP_001185806.1, NM_001198877.1
NP_001185807.1, NM_001198878.1
NP_034194.1, NM_010064.4
UniGeneiMm.249479

Genome annotation databases

EnsembliENSMUST00000081710; ENSMUSP00000080410; ENSMUSG00000027012
ENSMUST00000112138; ENSMUSP00000107766; ENSMUSG00000027012
GeneIDi13427
KEGGimmu:13427
UCSCiuc008kai.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063231 mRNA Translation: AAC33446.1
AK088015 mRNA Translation: BAC40097.1
AK164437 mRNA Translation: BAE37788.1
AK168730 mRNA Translation: BAE40571.1
CCDSiCCDS16112.1
RefSeqiNP_001185805.1, NM_001198876.1
NP_001185806.1, NM_001198877.1
NP_001185807.1, NM_001198878.1
NP_034194.1, NM_010064.4
UniGeneiMm.249479

3D structure databases

ProteinModelPortaliO88487
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199256, 9 interactors
IntActiO88487, 12 interactors
MINTiO88487
STRINGi10090.ENSMUSP00000097605

PTM databases

iPTMnetiO88487
PhosphoSitePlusiO88487

2D gel databases

REPRODUCTION-2DPAGEiO88487

Proteomic databases

EPDiO88487
MaxQBiO88487
PaxDbiO88487
PRIDEiO88487

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081710; ENSMUSP00000080410; ENSMUSG00000027012
ENSMUST00000112138; ENSMUSP00000107766; ENSMUSG00000027012
GeneIDi13427
KEGGimmu:13427
UCSCiuc008kai.2 mouse

Organism-specific databases

CTDi1781
MGIiMGI:107750 Dync1i2

Phylogenomic databases

eggNOGiKOG1587 Eukaryota
ENOG410XQ99 LUCA
GeneTreeiENSGT00730000110228
HOVERGENiHBG004083
InParanoidiO88487
KOiK10415
OMAiTIFPCHR
TreeFamiTF300553

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2132295 MHC class II antigen presentation
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-MMU-68877 Mitotic Prometaphase
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

PROiPR:O88487
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027012 Expressed in 315 organ(s), highest expression level in brain
CleanExiMM_DYNC1I2
ExpressionAtlasiO88487 baseline and differential
GenevisibleiO88487 MM

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR025956 DYNC1I1/DYNC1I2
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF11540 Dynein_IC2, 1 hit
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDC1I2_MOUSE
AccessioniPrimary (citable) accession number: O88487
Secondary accession number(s): Q3TGH7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 151 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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