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Protein

Cytoplasmic dynein 1 intermediate chain 1

Gene

Dync1i1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCNT1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMotor protein
Biological processTransport

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2132295 MHC class II antigen presentation
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-3371497 HSP90 chaperone cycle for steroid hormone receptors (SHR)
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-6807878 COPI-mediated anterograde transport
R-MMU-6811436 COPI-independent Golgi-to-ER retrograde traffic
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Cytoplasmic dynein 1 intermediate chain 1
Alternative name(s):
Cytoplasmic dynein intermediate chain 1
Dynein intermediate chain 1, cytosolic
Short name:
DH IC-1
Gene namesi
Name:Dync1i1
Synonyms:Dnci1, Dncic1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:107743 Dync1i1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Dynein, Kinetochore, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi151 – 155Missing : Abolishes interaction with DYNLL2. 1 Publication5
Mutagenesisi153T → A: Impairs interaction with DYNLL2. 1
Mutagenesisi153T → G: Abolishes interaction with DYNLL2. 1
Mutagenesisi154Q → A: Abolishes interaction with DYNLL2. 1
Mutagenesisi155T → G: Abolishes interaction with DYNLL2. 1
Mutagenesisi155T → S: Impairs interaction with DYNLL2. 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001146532 – 628Cytoplasmic dynein 1 intermediate chain 1Add BLAST627

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei50PhosphoserineCombined sources1
Modified residuei83PhosphoserineBy similarity1
Modified residuei88PhosphothreonineBy similarity1
Modified residuei90PhosphoserineBy similarity1
Modified residuei94PhosphoserineCombined sources1
Modified residuei97PhosphoserineCombined sources1
Modified residuei159PhosphothreonineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei180PhosphoserineCombined sources1
Modified residuei618PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO88485
PaxDbiO88485
PeptideAtlasiO88485
PRIDEiO88485

PTM databases

iPTMnetiO88485
PhosphoSitePlusiO88485
SwissPalmiO88485

Expressioni

Gene expression databases

BgeeiENSMUSG00000029757
CleanExiMM_DYNC1I1
ExpressionAtlasiO88485 baseline and differential
GenevisibleiO88485 MM

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and the LCs assemble on the IC dimer. Interacts with DYNC1H1. Interacts with DYNLT1 and DYNLT3. Interacts with DCNT1 (By similarity). Interacts with DYNLL2.By similarity1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199255, 10 interactors
CORUMiO88485
DIPiDIP-32048N
ELMiO88485
IntActiO88485, 14 interactors
MINTiO88485
STRINGi10090.ENSMUSP00000111221

Structurei

3D structure databases

ProteinModelPortaliO88485
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati268 – 317WD 1Add BLAST50
Repeati321 – 361WD 2Add BLAST41
Repeati370 – 411WD 3Add BLAST42
Repeati420 – 460WD 4Add BLAST41
Repeati465 – 510WD 5Add BLAST46
Repeati513 – 553WD 6Add BLAST41
Repeati559 – 598WD 7Add BLAST40

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 146Interaction with DYNLT1By similarityAdd BLAST17

Sequence similaritiesi

Belongs to the dynein intermediate chain family.Curated

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG1587 Eukaryota
ENOG410XQ99 LUCA
GeneTreeiENSGT00730000110228
HOGENOMiHOG000116383
HOVERGENiHBG004083
InParanoidiO88485
KOiK10415
TreeFamiTF300553

Family and domain databases

Gene3Di2.130.10.10, 2 hits
InterProiView protein in InterPro
IPR025956 DYNC1I1/DYNC1I2
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF11540 Dynein_IC2, 1 hit
PF00400 WD40, 1 hit
SMARTiView protein in SMART
SM00320 WD40, 5 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1A (identifier: O88485-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSDKSDLKAE LERKKQRLAQ IREEKKRKEE ERKKKEADMQ QKKEPVQDDS
60 70 80 90 100
DLDRKRRETE ALLQSIGISP EPPLVPTPMS PSSKSVSTPS DAGSQDSGDL
110 120 130 140 150
GPLTRTLQWD TDPSVLQLQS DSELGRRLHK LGVSKVTQVD FLPREVVSYS
160 170 180 190 200
KETQTPLATH QSEEDEEDEE MVEPKIGHDS ELENQEKKQE TKEAPPRELT
210 220 230 240 250
EEEKQQILHS EEFLIFFDRT IRVIERALAE DSDIFFDYSG RELEEKDGDV
260 270 280 290 300
QAGANLSFNR QFYDEHWSKH RVVTCMDWSL QYPELMVASY SNNEDAPHEP
310 320 330 340 350
DGVALVWNMK FKKTTPEYVF HCQSSVMSVC FARFHPNLVV GGTYSGQIVL
360 370 380 390 400
WDNRSHRRTP VQRTPLSAAA HTHPVYCVNV VGTQNAHNLI TVSTDGKMCS
410 420 430 440 450
WSLDMLSTPQ ESMELVYNKS KPVAVTGMAF PTGDVNNFVV GSEEGTVYTA
460 470 480 490 500
CRHGSKAGIG EVFEGHQGPV TGINCHMAVG PIDFSHLFVT SSFDWTVKLW
510 520 530 540 550
TTKHNKPLYS FEDNADYVYD VMWSPVHPAL FACVDGMGRL DLWNLNSDTE
560 570 580 590 600
VPTASVAIEG ASALNRVRWA QGGKEVAVGD SEGRIWIYDV GELAVPHNDE
610 620
WTRFARTLVE IRANRADSEE EGAVELAA
Length:628
Mass (Da):70,725
Last modified:July 27, 2011 - v2
Checksum:i1DFD9264D63B2CE8
GO
Isoform 1B (identifier: O88485-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     106-125: Missing.

Show »
Length:608
Mass (Da):68,510
Checksum:i3310615A8823774E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti384Q → H in AAC33444 (PubMed:10049579).Curated1
Sequence conflicti387H → R in AAC33444 (PubMed:10049579).Curated1
Sequence conflicti508L → V in AAC33444 (PubMed:10049579).Curated1
Sequence conflicti511F → S in AAC33444 (PubMed:10049579).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001334106 – 125Missing in isoform 1B. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063229 mRNA Translation: AAC33444.1
AF063230 mRNA Translation: AAC33445.1
CH466533 Genomic DNA Translation: EDL13960.1
BC051992 mRNA Translation: AAH51992.1
CCDSiCCDS19903.1 [O88485-1]
CCDS57408.1 [O88485-2]
RefSeqiNP_001177952.1, NM_001191023.1
NP_001177954.1, NM_001191025.1 [O88485-2]
NP_034193.2, NM_010063.4 [O88485-1]
UniGeneiMm.20893

Genome annotation databases

EnsembliENSMUST00000115554; ENSMUSP00000111216; ENSMUSG00000029757 [O88485-2]
ENSMUST00000115559; ENSMUSP00000111221; ENSMUSG00000029757 [O88485-1]
GeneIDi13426
KEGGimmu:13426
UCSCiuc009awm.2 mouse [O88485-2]
uc012ehw.1 mouse [O88485-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDC1I1_MOUSE
AccessioniPrimary (citable) accession number: O88485
Secondary accession number(s): O88486, Q80W09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: June 20, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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