UniProtKB - O88477 (IF2B1_MOUSE)
Insulin-like growth factor 2 mRNA-binding protein 1
Igf2bp1
Functioni
GO - Molecular functioni
- mRNA 3'-UTR binding Source: UniProtKB
- mRNA 5'-UTR binding Source: MGI
- mRNA binding Source: UniProtKB
- translation regulator activity Source: MGI
GO - Biological processi
- CRD-mediated mRNA stabilization Source: MGI
- mRNA transport Source: UniProtKB-KW
- negative regulation of translation Source: MGI
- nervous system development Source: GO_Central
- neuronal stem cell population maintenance Source: MGI
- pallium cell proliferation in forebrain Source: MGI
- regulation of gene expression Source: GO_Central
- regulation of mRNA stability involved in response to stress Source: UniProtKB
- regulation of RNA metabolic process Source: GO_Central
- RNA localization Source: MGI
Keywordsi
Molecular function | RNA-binding |
Biological process | mRNA transport, Translation regulation, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Insulin-like growth factor 2 mRNA-binding protein 1Short name: IGF2 mRNA-binding protein 1 Short name: IMP-1 Alternative name(s): Coding region determinant-binding protein Short name: CRD-BP IGF-II mRNA-binding protein 1 VICKZ family member 1 Zipcode-binding protein 1 Short name: ZBP-1 |
Gene namesi | Name:Igf2bp1 Synonyms:Vickz1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1890357, Igf2bp1 |
Subcellular locationi
Nucleus
Other locations
- Cytoplasm
- perinuclear region By similarity
- lamellipodium By similarity
- dendrite By similarity
- dendritic spine By similarity
- growth cone By similarity
- filopodium By similarity
- axon By similarity
Note: In the nucleus, located in discrete foci, coinciding with the sites of ACTB transcription (By similarity). In the cytoplasm, localizes in cytoplasmic mRNP granules. Colocalizes with microtubules in growth cone filopodia and along neurites in neuronal cells (By similarity). Cytoplasmic colocalization with ACTB mRNA is partially lost at the cell periphery, suggesting release of the transcript (By similarity). In hippocampal neurons, predominantly located within dendrites, particularly at dendritic branching points in young cells, compared to axons (By similarity). In axons, predominantly found in axonal branches and their growth cones (By similarity). In neuronal processes, exhibits fast retrograde and anterograde movements, when associated with ACTB mRNA; this motility is lost when the association is inhibited (By similarity). Dendritic levels are regulated by neuronal activity and glutaminergic signals: they are increased by KCl-induced depolarization, which induces rapid efflux from the cell body into dendrites, and decreased by NMDA receptor agonists (By similarity). In motile cells, such as migrating fibroblasts, localizes to leading edges where it colocalizes with microtubules and microfilaments and to retracting tails (By similarity). In motile cells, transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). In response to cellular stress, such as oxidative stress or heat shock, recruited to stress granules, but not to processing bodies (By similarity).By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- CRD-mediated mRNA stability complex Source: UniProtKB
- cytoplasm Source: MGI
- cytoplasmic stress granule Source: UniProtKB
- dendrite Source: MGI
- dendritic spine Source: UniProtKB-SubCell
- filopodium Source: UniProtKB-SubCell
- growth cone Source: UniProtKB-SubCell
- lamellipodium Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, Nucleus, SynapsePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000282534 | 1 – 577 | Insulin-like growth factor 2 mRNA-binding protein 1Add BLAST | 577 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphoserineBy similarity | 1 | |
Modified residuei | 73 | PhosphoserineBy similarity | 1 | |
Modified residuei | 181 | PhosphoserineCombined sources | 1 | |
Modified residuei | 528 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
MaxQBi | O88477 |
PaxDbi | O88477 |
PeptideAtlasi | O88477 |
PRIDEi | O88477 |
PTM databases
iPTMneti | O88477 |
PhosphoSitePlusi | O88477 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000013415, Expressed in brain and 163 other tissues |
Genevisiblei | O88477, MM |
Interactioni
Subunit structurei
Can form homodimers and heterodimers with IGF2BP1 and IGF2BP3 (By similarity).
Component of the coding region determinant (CRD)-mediated complex, composed of DHX9, HNRNPU, IGF2BP1, SYNCRIP and YBX1 (By similarity).
Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1 (By similarity). Associates with mRNP complex (By similarity).
Interacts with FMR1 (By similarity).
Component of a multisubunit autoregulatory RNP complex (ARC), at least composed of IGF2BP1, PABPC1 and CSDE1.
Interacts with AGO1 and AGO2 (By similarity).
Interacts, through domains KH3 and KH4, with PABPC1 in an RNA-independent manner (By similarity).
Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP.
Interacts with ELAVL4 in an RNA-dependent manner. Associates with microtubules and polysomes.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 228260, 12 interactors |
ComplexPortali | CPX-1089, CRD-mediated mRNA stability complex |
DIPi | DIP-48578N |
IntActi | O88477, 5 interactors |
MINTi | O88477 |
STRINGi | 10090.ENSMUSP00000013559 |
Miscellaneous databases
RNActi | O88477, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 75 | RRM 1PROSITE-ProRule annotationAdd BLAST | 74 | |
Domaini | 81 – 156 | RRM 2PROSITE-ProRule annotationAdd BLAST | 76 | |
Domaini | 195 – 260 | KH 1PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 276 – 343 | KH 2PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 405 – 470 | KH 3PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 487 – 553 | KH 4PROSITE-ProRule annotationAdd BLAST | 67 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 187 – 570 | Necessary for interaction with IGF2BP1 and binding to TAU mRNAAdd BLAST | 384 | |
Regioni | 312 – 323 | Sufficient for nuclear exportBy similarityAdd BLAST | 12 | |
Regioni | 485 – 495 | Sufficient for nuclear exportBy similarityAdd BLAST | 11 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2193, Eukaryota |
GeneTreei | ENSGT00940000160427 |
HOGENOMi | CLU_020744_1_0_1 |
InParanoidi | O88477 |
OMAi | ISQGPEN |
OrthoDBi | 286875at2759 |
PhylomeDBi | O88477 |
TreeFami | TF320229 |
Family and domain databases
CDDi | cd12625, RRM1_IGF2BP1, 1 hit cd12628, RRM2_IGF2BP1, 1 hit |
Gene3Di | 3.30.1370.10, 2 hits 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034837, IGF2BP1_RRM1 IPR034842, IGF2BP1_RRM2 IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00013, KH_1, 4 hits PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00322, KH, 4 hits SM00360, RRM, 2 hits |
SUPFAMi | SSF54791, SSF54791, 4 hits SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 4 hits PS50102, RRM, 2 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MNKLYIGNLN ESVTPADLEK VFAEHKISYS GQFLVKSGYA FVDCPDEHWA
60 70 80 90 100
MKAIETFSGK VELQGKRLEI EHSVPKKQRS RKIQIRNIPP QLRWEVLDSL
110 120 130 140 150
LAQYGTVENC EQVNTESETA VVNVTYSNRE QTRQAIMKLN GHQLENHALK
160 170 180 190 200
VSYIPDEQIT QGPENGRRGG FGSRGQPRQG SPVAAGAPAK QQPVDIPLRL
210 220 230 240 250
LVPTQYVGAI IGKEGATIRN ITKQTQSKID VHRKENAGAA EKAISVHSTP
260 270 280 290 300
EGCSSACKMI LEIMHKEAKD TKTADEVPLK ILAHNNFVGR LIGKEGRNLK
310 320 330 340 350
KVEQDTETKI TISSLQDLTL YNPERTITVK GAIENCCRAE QEIMKKVREA
360 370 380 390 400
YENDVAAMSL QSHLIPGLNL AAVGLFPASS SAVPPPPSSV TGAAPYSSFM
410 420 430 440 450
QAPEQEMVQV FIPAQAVGAI IGKKGQHIKQ LSRFASASIK IAPPETPDSK
460 470 480 490 500
VRMVVITGPP EAQFKAQGRI YGKLKEENFF GPKEEVKLET HIRVPASAAG
510 520 530 540 550
RVIGKGGKTV NELQNLTAAE VVVPRDQTPD ENDQVIVKII GHFYASQMAQ
560 570
RKIRDILAQV KQQHQKGQSN LAQARRK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 276 | E → G in AAH51679 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 406 | E → G in BAC32119 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061569 mRNA Translation: AAC72743.1 AK044850 mRNA Translation: BAC32119.1 AK013940 mRNA Translation: BAB29071.1 AL603682 Genomic DNA No translation available. AL606704 Genomic DNA No translation available. BC051679 mRNA Translation: AAH51679.1 |
CCDSi | CCDS25286.1 |
RefSeqi | NP_034081.1, NM_009951.4 |
Genome annotation databases
Ensembli | ENSMUST00000013559; ENSMUSP00000013559; ENSMUSG00000013415 |
GeneIDi | 140486 |
KEGGi | mmu:140486 |
UCSCi | uc007lay.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF061569 mRNA Translation: AAC72743.1 AK044850 mRNA Translation: BAC32119.1 AK013940 mRNA Translation: BAB29071.1 AL603682 Genomic DNA No translation available. AL606704 Genomic DNA No translation available. BC051679 mRNA Translation: AAH51679.1 |
CCDSi | CCDS25286.1 |
RefSeqi | NP_034081.1, NM_009951.4 |
3D structure databases
SMRi | O88477 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228260, 12 interactors |
ComplexPortali | CPX-1089, CRD-mediated mRNA stability complex |
DIPi | DIP-48578N |
IntActi | O88477, 5 interactors |
MINTi | O88477 |
STRINGi | 10090.ENSMUSP00000013559 |
PTM databases
iPTMneti | O88477 |
PhosphoSitePlusi | O88477 |
Proteomic databases
MaxQBi | O88477 |
PaxDbi | O88477 |
PeptideAtlasi | O88477 |
PRIDEi | O88477 |
Protocols and materials databases
Antibodypediai | 17915, 304 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000013559; ENSMUSP00000013559; ENSMUSG00000013415 |
GeneIDi | 140486 |
KEGGi | mmu:140486 |
UCSCi | uc007lay.2, mouse |
Organism-specific databases
CTDi | 10642 |
MGIi | MGI:1890357, Igf2bp1 |
Phylogenomic databases
eggNOGi | KOG2193, Eukaryota |
GeneTreei | ENSGT00940000160427 |
HOGENOMi | CLU_020744_1_0_1 |
InParanoidi | O88477 |
OMAi | ISQGPEN |
OrthoDBi | 286875at2759 |
PhylomeDBi | O88477 |
TreeFami | TF320229 |
Miscellaneous databases
BioGRID-ORCSi | 140486, 2 hits in 17 CRISPR screens |
ChiTaRSi | Igf2bp1, mouse |
PROi | PR:O88477 |
RNActi | O88477, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000013415, Expressed in brain and 163 other tissues |
Genevisiblei | O88477, MM |
Family and domain databases
CDDi | cd12625, RRM1_IGF2BP1, 1 hit cd12628, RRM2_IGF2BP1, 1 hit |
Gene3Di | 3.30.1370.10, 2 hits 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR034837, IGF2BP1_RRM1 IPR034842, IGF2BP1_RRM2 IPR004087, KH_dom IPR004088, KH_dom_type_1 IPR036612, KH_dom_type_1_sf IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom |
Pfami | View protein in Pfam PF00013, KH_1, 4 hits PF00076, RRM_1, 2 hits |
SMARTi | View protein in SMART SM00322, KH, 4 hits SM00360, RRM, 2 hits |
SUPFAMi | SSF54791, SSF54791, 4 hits SSF54928, SSF54928, 1 hit |
PROSITEi | View protein in PROSITE PS50084, KH_TYPE_1, 4 hits PS50102, RRM, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IF2B1_MOUSE | |
Accessioni | O88477Primary (citable) accession number: O88477 Secondary accession number(s): Q80US9, Q8BRH1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families