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Protein

Calpain small subunit 1

Gene

Capns1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi110Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi113Calcium 1By similarity1
Metal bindingi115Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi120Calcium 1By similarity1
Metal bindingi138Calcium 4By similarity1
Metal bindingi153Calcium 2By similarity1
Metal bindingi155Calcium 2By similarity1
Metal bindingi157Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi159Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi164Calcium 2By similarity1
Metal bindingi183Calcium 3By similarity1
Metal bindingi185Calcium 3By similarity1
Metal bindingi187Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi189Calcium 3; via carbonyl oxygenBy similarity1
Metal bindingi194Calcium 3By similarity1
Metal bindingi226Calcium 4By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi109 – 1201PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi153 – 1642PROSITE-ProRule annotationAdd BLAST12
Calcium bindingi183 – 1943PROSITE-ProRule annotationAdd BLAST12

GO - Molecular functioni

  • calcium-dependent cysteine-type endopeptidase activity Source: MGI
  • calcium ion binding Source: InterPro
  • protein heterodimerization activity Source: MGI

Keywordsi

LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.B24 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain small subunit 1
Short name:
CSS1
Alternative name(s):
Calcium-activated neutral proteinase small subunit
Short name:
CANP small subunit
Calcium-dependent protease small subunit
Short name:
CDPS
Calcium-dependent protease small subunit 1
Calpain regulatory subunit
Gene namesi
Name:Capns1
Synonyms:Capn4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:88266 Capns1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000737141 – 269Calpain small subunit 1Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei6PhosphoserineBy similarity1
Modified residuei180N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO88456
MaxQBiO88456
PaxDbiO88456
PeptideAtlasiO88456
PRIDEiO88456

PTM databases

iPTMnetiO88456
PhosphoSitePlusiO88456

Expressioni

Gene expression databases

CleanExiMM_CAPNS1

Interactioni

Subunit structurei

Homodimer or heterodimer of a large (catalytic) and a small (regulatory) subunit. In presence of calcium, the heterodimer dissociates (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198473, 2 interactors
IntActiO88456, 2 interactors
MINTiO88456
STRINGi10090.ENSMUSP00000001845

Structurei

3D structure databases

ProteinModelPortaliO88456
SMRiO88456
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini97 – 131EF-hand 1; atypicalPROSITE-ProRule annotationAdd BLAST35
Domaini140 – 173EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini170 – 205EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini206 – 234EF-hand 4PROSITE-ProRule annotationAdd BLAST29
Domaini235 – 269EF-hand 5PROSITE-ProRule annotationAdd BLAST35

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1 – 65Gly-rich (hydrophobic)Add BLAST65
Compositional biasi10 – 25Poly-GlyAdd BLAST16
Compositional biasi37 – 57Poly-GlyAdd BLAST21
Compositional biasi79 – 84Poly-Pro6

Domaini

The contact of the 5th EF-hand domain from each monomer allows the formation of the homodimer and also appears to mediate the contact between the large catalytic subunit and small regulatory subunit for the formation of the heterodimer.By similarity
EF-hand domains are paired. EF-hand 1 is paired with EF-hand 2 and EF-hand 3 is paired with EF-hand 4. The fifth EF-hand domain, left unpaired, does not bind the calcium but is responsible of the dimerization by EF-embrace. The first four EF-hand domains bind calcium, however it is not sure if the binding of EF-hand 4 to calcium is physiologically relevant.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0037 Eukaryota
ENOG410YKQK LUCA
HOGENOMiHOG000063658
HOVERGENiHBG004492
InParanoidiO88456
KOiK08583
PhylomeDBiO88456
TreeFamiTF314682

Family and domain databases

InterProiView protein in InterPro
IPR029642 CAPN4
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PANTHERiPTHR44408:SF3 PTHR44408:SF3, 1 hit
PfamiView protein in Pfam
PF13833 EF-hand_8, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O88456-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFLVNSFLKG GGGGGGGGGL GGGLGNVLGG LISGAAGGGG GGGGGGMGLG
60 70 80 90 100
GGGGGGGTAM RILGGVISAI SEAAAQYNPE PPPPRSHYSN IEANESEEVR
110 120 130 140 150
QFRKLFVQLA GDDMEVSATE LMNILNKVVT RHPDLKTDGF GIDTCRSMVA
160 170 180 190 200
VMDSDTTGKL GFEEFKYLWN NIKKWQAIYK RFDTDRSGTI GSHELPGAFE
210 220 230 240 250
AAGFHLNEHL YSMIIRRYAD ESGNMDFDNF ISCLVRLDAM FRAFKSLDKN
260
GTGQIQVNIQ EWLQLTMYS
Length:269
Mass (Da):28,463
Last modified:November 1, 1998 - v1
Checksum:iC578771942FB57E9
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0R4IZW8A0A0R4IZW8_MOUSE
Calpain small subunit 1
Capns1 mCG_21569
268Annotation score:
A0A0R4J1C2A0A0R4J1C2_MOUSE
Calpain small subunit 1
Capns1
200Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46Missing in AAH18352 (PubMed:15489334).Curated1
Sequence conflicti46Missing in AAH90988 (PubMed:15489334).Curated1
Sequence conflicti69 – 70AI → PL in BAC25743 (PubMed:16141072).Curated2
Sequence conflicti70I → M in BAE21378 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139373 Genomic DNA Translation: AAD38363.2
AF058298 mRNA Translation: AAC97194.1
BC090988 mRNA Translation: AAH90988.1
BC018352 mRNA Translation: AAH18352.1
AK028092 mRNA Translation: BAC25743.1
AK132819 mRNA Translation: BAE21378.1
RefSeqiNP_033925.2, NM_009795.4
XP_006539551.1, XM_006539488.2
UniGeneiMm.439782

Genome annotation databases

GeneIDi12336
KEGGimmu:12336
UCSCiuc009gdq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139373 Genomic DNA Translation: AAD38363.2
AF058298 mRNA Translation: AAC97194.1
BC090988 mRNA Translation: AAH90988.1
BC018352 mRNA Translation: AAH18352.1
AK028092 mRNA Translation: BAC25743.1
AK132819 mRNA Translation: BAE21378.1
RefSeqiNP_033925.2, NM_009795.4
XP_006539551.1, XM_006539488.2
UniGeneiMm.439782

3D structure databases

ProteinModelPortaliO88456
SMRiO88456
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198473, 2 interactors
IntActiO88456, 2 interactors
MINTiO88456
STRINGi10090.ENSMUSP00000001845

PTM databases

iPTMnetiO88456
PhosphoSitePlusiO88456

Proteomic databases

EPDiO88456
MaxQBiO88456
PaxDbiO88456
PeptideAtlasiO88456
PRIDEiO88456

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi12336
KEGGimmu:12336
UCSCiuc009gdq.1 mouse

Organism-specific databases

CTDi826
MGIiMGI:88266 Capns1

Phylogenomic databases

eggNOGiKOG0037 Eukaryota
ENOG410YKQK LUCA
HOGENOMiHOG000063658
HOVERGENiHBG004492
InParanoidiO88456
KOiK08583
PhylomeDBiO88456
TreeFamiTF314682

Enzyme and pathway databases

BRENDAi3.4.22.B24 3474

Miscellaneous databases

ChiTaRSiCapns1 mouse
PROiPR:O88456
SOURCEiSearch...

Gene expression databases

CleanExiMM_CAPNS1

Family and domain databases

InterProiView protein in InterPro
IPR029642 CAPN4
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
PANTHERiPTHR44408:SF3 PTHR44408:SF3, 1 hit
PfamiView protein in Pfam
PF13833 EF-hand_8, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCPNS1_MOUSE
AccessioniPrimary (citable) accession number: O88456
Secondary accession number(s): Q3V0X5
, Q5BKQ2, Q8CEI2, Q8VEK4, Q9R1C5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: November 1, 1998
Last modified: July 18, 2018
This is version 161 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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