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UniProtKB - O88455 (DHCR7_MOUSE)
Protein
7-dehydrocholesterol reductase
Gene
Dhcr7
Organism
Mus musculus (Mouse)
Status
Functioni
7-dehydrocholesterol reductase of the cholesterol biosynthetic pathway reducing the C7-C8 double bond of cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol, two intermediates in that pathway.
1 PublicationCatalytic activityi
- EC:1.3.1.211 PublicationThis reaction proceeds in the backward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
: cholesterol biosynthesis Pathwayi
This protein is involved in the pathway cholesterol biosynthesis, which is part of Steroid biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway cholesterol biosynthesis and in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 354 | NADPBy similarity | 1 | |
Binding sitei | 358 | NADPBy similarity | 1 | |
Binding sitei | 391 | NADP; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 396 | NADPBy similarity | 1 | |
Binding sitei | 443 | NADPBy similarity | 1 | |
Binding sitei | 458 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 403 – 404 | NADPBy similarity | 2 | |
Nucleotide bindingi | 447 – 451 | NADPBy similarity | 5 |
GO - Molecular functioni
- 7-dehydrocholesterol reductase activity Source: MGI
- NADP binding Source: UniProtKB
- oxidoreductase activity, acting on the CH-CH group of donors Source: GO_Central
- sterol delta7 reductase activity Source: GO_Central
GO - Biological processi
- blood vessel development Source: MGI
- brassinosteroid biosynthetic process Source: GO_Central
- cell differentiation Source: MGI
- cholesterol biosynthetic process Source: MGI
- lung development Source: MGI
- multicellular organism growth Source: MGI
- post-embryonic development Source: MGI
- regulation of cell population proliferation Source: MGI
- regulation of cholesterol biosynthetic process Source: MGI
- sterol biosynthetic process Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.3.1.21, 3474 |
Reactomei | R-MMU-6807047, Cholesterol biosynthesis via desmosterol R-MMU-6807062, Cholesterol biosynthesis via lathosterol |
UniPathwayi | UPA00063 |
Chemistry databases
SwissLipidsi | SLP:000001320 |
Names & Taxonomyi
Protein namesi | Recommended name: 7-dehydrocholesterol reductase (EC:1.3.1.211 Publication)Short name: 7-DHC reductase Alternative name(s): Sterol Delta(7)-reductase |
Gene namesi | Name:Dhcr7 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1298378, Dhcr7 |
VEuPathDBi | HostDB:ENSMUSG00000058454 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- integral component of endoplasmic reticulum membrane Source: GO_Central
Nucleus
- nuclear outer membrane Source: MGI
Other locations
- intracellular membrane-bounded organelle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 36 – 56 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 95 – 115 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 144 – 164 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 173 – 193 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 233 – 253 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 262 – 282 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 302 – 322 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 327 – 347 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 416 – 436 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Disruption phenotypei
Deficient mice die within one day of birth due to respiratory and suckling problems. They exhibit abnormal cholesterol homeostasis with reduced tissue cholesterol levels and total sterol levels, enlarged bladders and sometimes cleft palate.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000207503 | 1 – 471 | 7-dehydrocholesterol reductaseAdd BLAST | 471 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 14 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O88455 |
jPOSTi | O88455 |
MaxQBi | O88455 |
PaxDbi | O88455 |
PeptideAtlasi | O88455 |
PRIDEi | O88455 |
ProteomicsDBi | 277332 |
TopDownProteomicsi | O88455 |
PTM databases
iPTMneti | O88455 |
PhosphoSitePlusi | O88455 |
SwissPalmi | O88455 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000058454, Expressed in adrenal gland and 307 other tissues |
Genevisiblei | O88455, MM |
Interactioni
Subunit structurei
Interacts with DHCR24; this interaction regulates DHCR7 activity.
By similarityProtein-protein interaction databases
BioGRIDi | 199217, 2 interactors |
STRINGi | 10090.ENSMUSP00000073541 |
Miscellaneous databases
RNActi | O88455, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 23 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 21 | Polar residuesSequence analysisAdd BLAST | 21 |
Sequence similaritiesi
Belongs to the ERG4/ERG24 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1435, Eukaryota |
GeneTreei | ENSGT00390000000417 |
HOGENOMi | CLU_015631_0_0_1 |
InParanoidi | O88455 |
PhylomeDBi | O88455 |
TreeFami | TF101180 |
Family and domain databases
InterProi | View protein in InterPro IPR001171, Ergosterol_biosynth_ERG4_ERG24 IPR018083, Sterol_reductase_CS |
Pfami | View protein in Pfam PF01222, ERG4_ERG24, 1 hit |
PROSITEi | View protein in PROSITE PS01017, STEROL_REDUCT_1, 1 hit PS01018, STEROL_REDUCT_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All
O88455-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASKSQHNAP KVKSPNGKAG SQGQWGRAWE VDWFSLASII FLLLFAPFIV
60 70 80 90 100
YYFIMACDQY SCSLTAPALD IATGHASLAD IWAKTPPVTA KAAQLYALWV
110 120 130 140 150
SFQVLLYSWL PDFCHRFLPG YVGGVQEGAI TPAGVVNKYE VNGLQAWLIT
160 170 180 190 200
HILWFVNAYL LSWFSPTIIF DNWIPLLWCA NILGYAVSTF AMIKGYLFPT
210 220 230 240 250
SAEDCKFTGN FFYNYMMGIE FNPRIGKWFD FKLFFNGRPG IVAWTLINLS
260 270 280 290 300
FAAKQQELYG HVTNSMILVN VLQAIYVLDF FWNETWYLKT IDICHDHFGW
310 320 330 340 350
YLGWGDCVWL PYLYTLQGLY LVYHPVQLST PNALGILLLG LVGYYIFRMT
360 370 380 390 400
NHQKDLFRRT DGRCLIWGKK PKAIECSYTS ADGLKHHSKL LVSGFWGVAR
410 420 430 440 450
HFNYTGDLMG SLAYCLACGG GHLLPYFYII YMTILLTHRC LRDEHRCANK
460 470
YGRDWERYTA AVPYRLLPGI F
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A140LIT2 | A0A140LIT2_MOUSE | 7-dehydrocholesterol reductase | Dhcr7 | 474 | Annotation score: | ||
D3YXR4 | D3YXR4_MOUSE | 7-dehydrocholesterol reductase | Dhcr7 | 225 | Annotation score: | ||
D3Z7G1 | D3Z7G1_MOUSE | 7-dehydrocholesterol reductase | Dhcr7 | 215 | Annotation score: | ||
A0A140LI18 | A0A140LI18_MOUSE | 7-dehydrocholesterol reductase | Dhcr7 | 71 | Annotation score: | ||
Q711T1 | Q711T1_MOUSE | 7-dehydrocholesterol reductase | Dhcr7 | 12 | Annotation score: | ||
A0A140LIS4 | A0A140LIS4_MOUSE | 7-dehydrocholesterol reductase | Dhcr7 | 23 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF057368 mRNA Translation: AAC40164.1 BC006854 mRNA Translation: AAH06854.1 |
CCDSi | CCDS22048.1 |
RefSeqi | NP_031882.1, NM_007856.2 XP_006508541.1, XM_006508478.1 XP_006508542.1, XM_006508479.3 XP_006508543.1, XM_006508480.2 XP_006508544.1, XM_006508481.2 |
Genome annotation databases
Ensembli | ENSMUST00000073878; ENSMUSP00000073541; ENSMUSG00000058454 ENSMUST00000124340; ENSMUSP00000117659; ENSMUSG00000058454 ENSMUST00000141916; ENSMUSP00000121782; ENSMUSG00000058454 |
GeneIDi | 13360 |
KEGGi | mmu:13360 |
UCSCi | uc009kqc.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF057368 mRNA Translation: AAC40164.1 BC006854 mRNA Translation: AAH06854.1 |
CCDSi | CCDS22048.1 |
RefSeqi | NP_031882.1, NM_007856.2 XP_006508541.1, XM_006508478.1 XP_006508542.1, XM_006508479.3 XP_006508543.1, XM_006508480.2 XP_006508544.1, XM_006508481.2 |
3D structure databases
AlphaFoldDBi | O88455 |
SMRi | O88455 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 199217, 2 interactors |
STRINGi | 10090.ENSMUSP00000073541 |
Chemistry databases
SwissLipidsi | SLP:000001320 |
PTM databases
iPTMneti | O88455 |
PhosphoSitePlusi | O88455 |
SwissPalmi | O88455 |
Proteomic databases
EPDi | O88455 |
jPOSTi | O88455 |
MaxQBi | O88455 |
PaxDbi | O88455 |
PeptideAtlasi | O88455 |
PRIDEi | O88455 |
ProteomicsDBi | 277332 |
TopDownProteomicsi | O88455 |
Protocols and materials databases
Antibodypediai | 30720, 175 antibodies from 26 providers |
DNASUi | 13360 |
Genome annotation databases
Ensembli | ENSMUST00000073878; ENSMUSP00000073541; ENSMUSG00000058454 ENSMUST00000124340; ENSMUSP00000117659; ENSMUSG00000058454 ENSMUST00000141916; ENSMUSP00000121782; ENSMUSG00000058454 |
GeneIDi | 13360 |
KEGGi | mmu:13360 |
UCSCi | uc009kqc.1, mouse |
Organism-specific databases
CTDi | 1717 |
MGIi | MGI:1298378, Dhcr7 |
VEuPathDBi | HostDB:ENSMUSG00000058454 |
Phylogenomic databases
eggNOGi | KOG1435, Eukaryota |
GeneTreei | ENSGT00390000000417 |
HOGENOMi | CLU_015631_0_0_1 |
InParanoidi | O88455 |
PhylomeDBi | O88455 |
TreeFami | TF101180 |
Enzyme and pathway databases
UniPathwayi | UPA00063 |
BRENDAi | 1.3.1.21, 3474 |
Reactomei | R-MMU-6807047, Cholesterol biosynthesis via desmosterol R-MMU-6807062, Cholesterol biosynthesis via lathosterol |
Miscellaneous databases
BioGRID-ORCSi | 13360, 3 hits in 76 CRISPR screens |
ChiTaRSi | Dhcr7, mouse |
PROi | PR:O88455 |
RNActi | O88455, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058454, Expressed in adrenal gland and 307 other tissues |
Genevisiblei | O88455, MM |
Family and domain databases
InterProi | View protein in InterPro IPR001171, Ergosterol_biosynth_ERG4_ERG24 IPR018083, Sterol_reductase_CS |
Pfami | View protein in Pfam PF01222, ERG4_ERG24, 1 hit |
PROSITEi | View protein in PROSITE PS01017, STEROL_REDUCT_1, 1 hit PS01018, STEROL_REDUCT_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHCR7_MOUSE | |
Accessioni | O88455Primary (citable) accession number: O88455 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 31, 2002 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 156 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families