UniProtKB - O88428 (PAPS2_MOUSE)
Protein
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2
Gene
Papss2
Organism
Mus musculus (Mouse)
Status
Functioni
Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth.
Catalytic activityi
: sulfate assimilation Pathwayi
This protein is involved in the pathway sulfate assimilation, which is part of Sulfur metabolism.View all proteins of this organism that are known to be involved in the pathway sulfate assimilation and in Sulfur metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 92 | Adenylyl sulfateBy similarity | 1 | |
Binding sitei | 162 | Adenylyl sulfateBy similarity | 1 | |
Binding sitei | 198 | ATP 1; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 559 | ATP 2; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 53 – 58 | ATP 1By similarity | 6 | |
Nucleotide bindingi | 415 – 418 | ATP 2By similarity | 4 | |
Nucleotide bindingi | 517 – 521 | ATP 2By similarity | 5 |
GO - Molecular functioni
- adenylylsulfate kinase activity Source: MGI
- ATP binding Source: UniProtKB-KW
- nucleotidyltransferase activity Source: UniProtKB
- sulfate adenylyltransferase (ATP) activity Source: MGI
GO - Biological processi
- 3'-phosphoadenosine 5'-phosphosulfate biosynthetic process Source: GO_Central
- blood coagulation Source: MGI
- bone development Source: MGI
- sulfate assimilation Source: MGI
Keywordsi
Molecular function | Kinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-174362 Transport and synthesis of PAPS |
UniPathwayi | UPA00097 |
Names & Taxonomyi
Protein namesi | Recommended name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2Short name: PAPS synthase 2 Short name: PAPSS 2 Alternative name(s): Sulfurylase kinase 2 Short name: SK 2 Short name: SK2 Including the following 2 domains: Sulfate adenylyltransferase (EC:2.7.7.4) Alternative name(s): ATP-sulfurylase Sulfate adenylate transferase Short name: SAT Adenylyl-sulfate kinase (EC:2.7.1.25) Alternative name(s): 3'-phosphoadenosine-5'-phosphosulfate synthase APS kinase Adenosine-5'-phosphosulfate 3'-phosphotransferase Adenylylsulfate 3'-phosphotransferase |
Gene namesi | Name:Papss2 Synonyms:Atpsk2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1330223 Papss2 |
Pathology & Biotechi
Involvement in diseasei
Defects in Papss2 are the cause of brachymorphism (bm), a autosomal recessive disease, which is characterized by abnormal hepatic detoxification, bleeding times and postnatal growth, such as dome-shaped skull, short thick tail, and shortened but not widened limbs. The abnormal postnatal growth has been attributed to undersulfation of cartilage proteoglycans.
Keywords - Diseasei
Disease mutationPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000105962 | 1 – 621 | Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2Add BLAST | 621 |
Proteomic databases
jPOSTi | O88428 |
MaxQBi | O88428 |
PaxDbi | O88428 |
PeptideAtlasi | O88428 |
PRIDEi | O88428 |
PTM databases
iPTMneti | O88428 |
PhosphoSitePlusi | O88428 |
Expressioni
Tissue specificityi
Expressed in liver, cartilage, skin and brain.
Gene expression databases
Bgeei | ENSMUSG00000024899 Expressed in 331 organ(s), highest expression level in intestinal epithelium |
Genevisiblei | O88428 MM |
Interactioni
Protein-protein interaction databases
IntActi | O88428, 4 interactors |
MINTi | O88428 |
STRINGi | 10090.ENSMUSP00000025833 |
Structurei
3D structure databases
ProteinModelPortali | O88428 |
SMRi | O88428 |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 216 | Adenylyl-sulfate kinaseCuratedAdd BLAST | 216 | |
Regioni | 80 – 83 | Adenylyl sulfate bindingBy similarity | 4 | |
Regioni | 97 – 100 | Adenylyl sulfate bindingBy similarity | 4 | |
Regioni | 123 – 124 | Adenylyl sulfate bindingBy similarity | 2 | |
Regioni | 175 – 176 | Adenylyl sulfate bindingBy similarity | 2 | |
Regioni | 225 – 621 | Sulfate adenylyltransferaseCuratedAdd BLAST | 397 |
Sequence similaritiesi
In the N-terminal section; belongs to the APS kinase family.Curated
In the C-terminal section; belongs to the sulfate adenylyltransferase family.Curated
Phylogenomic databases
eggNOGi | KOG0635 Eukaryota KOG4238 Eukaryota COG0529 LUCA COG2046 LUCA |
GeneTreei | ENSGT00390000009613 |
HOGENOMi | HOG000069045 |
HOVERGENi | HBG053503 |
InParanoidi | O88428 |
KOi | K13811 |
OMAi | QHPYIKM |
OrthoDBi | 528280at2759 |
TreeFami | TF313143 |
Family and domain databases
CDDi | cd02027 APSK, 1 hit cd00517 ATPS, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00065 Adenylyl_sulf_kinase, 1 hit |
InterProi | View protein in InterPro IPR002891 APS_kinase IPR025980 ATP-Sase_PUA-like_dom IPR027417 P-loop_NTPase IPR015947 PUA-like_sf IPR014729 Rossmann-like_a/b/a_fold IPR024951 Sulfurylase_cat_dom IPR002650 Sulphate_adenylyltransferase |
Pfami | View protein in Pfam PF01747 ATP-sulfurylase, 1 hit PF14306 PUA_2, 1 hit |
SUPFAMi | SSF52540 SSF52540, 1 hit SSF88697 SSF88697, 1 hit |
TIGRFAMsi | TIGR00455 apsK, 1 hit TIGR00339 sopT, 1 hit |
i Sequence
Sequence statusi: Complete.
O88428-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSANFKMNHK RDQQKSTNVV YQAHHVSRNK RGQVVGTRGG FRGCTVWLTG
60 70 80 90 100
LSGAGKTTIS FALEEYLVSH AIPCYSLDGD NVRHGLNKNL GFSAGDREEN
110 120 130 140 150
IRRIAEVARL FADAGLVCIT SFISPFAKDR ENARKIHESA GLPFFEIFVD
160 170 180 190 200
APLNICESRD VKGLYKRARA GEIKGFTGID SDYEKPETPE CVLKTNLSSV
210 220 230 240 250
SDCVQQVVEL LQEQNIVPHT TIKGIHELFV PENKVDQIRA EAETLPSLPI
260 270 280 290 300
TKLDLQWVQI LSEGWATPLK GFMREKEYLQ TLHFDTLLDG VVPRDGVINM
310 320 330 340 350
SIPIVLPVSA DDKARLEGCS KFALMYEGRR VALLQDPEFY EHRKEERCSR
360 370 380 390 400
VWGTATAKHP HIKMVMESGD WLVGGDLQVL ERIRWDDGLD QYRLTPLELK
410 420 430 440 450
QKCKDMNADA VFAFQLRNPV HNGHALLMQD TRRRLLERGY KHPVLLLHPL
460 470 480 490 500
GGWTKDDDVP LEWRMKQHAA VLEERVLDPK STIVAIFPSP MLYAGPTEVQ
510 520 530 540 550
WHCRCRMIAG ANFYIVGRDP AGMPHPETKK DLYEPTHGGK VLSMAPGLTS
560 570 580 590 600
VEIIPFRVAA YNKIKKAMDF YDPARHEEFD FISGTRMRKL AREGEDPPDG
610 620
FMAPKAWKVL TDYYRSLEKT N
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | F → S in AAC40191 (PubMed:9671738).Curated | 1 | |
Sequence conflicti | 290 – 294 | Missing in AAC98687 (PubMed:9771708).Curated | 5 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 79 | G → R in bm; activity abolished. 2 Publications | 1 | |
Natural varianti | 109 | R → K1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052453 mRNA Translation: AAC40191.1 AF085144 mRNA Translation: AAC98687.1 BC090997 mRNA Translation: AAH90997.1 |
CCDSi | CCDS37960.1 |
RefSeqi | NP_035994.2, NM_011864.3 |
UniGenei | Mm.203916 |
Genome annotation databases
Ensembli | ENSMUST00000025833; ENSMUSP00000025833; ENSMUSG00000024899 |
GeneIDi | 23972 |
KEGGi | mmu:23972 |
UCSCi | uc008hfl.2 mouse |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF052453 mRNA Translation: AAC40191.1 AF085144 mRNA Translation: AAC98687.1 BC090997 mRNA Translation: AAH90997.1 |
CCDSi | CCDS37960.1 |
RefSeqi | NP_035994.2, NM_011864.3 |
UniGenei | Mm.203916 |
3D structure databases
ProteinModelPortali | O88428 |
SMRi | O88428 |
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
IntActi | O88428, 4 interactors |
MINTi | O88428 |
STRINGi | 10090.ENSMUSP00000025833 |
PTM databases
iPTMneti | O88428 |
PhosphoSitePlusi | O88428 |
Proteomic databases
jPOSTi | O88428 |
MaxQBi | O88428 |
PaxDbi | O88428 |
PeptideAtlasi | O88428 |
PRIDEi | O88428 |
Protocols and materials databases
DNASUi | 23972 |
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000025833; ENSMUSP00000025833; ENSMUSG00000024899 |
GeneIDi | 23972 |
KEGGi | mmu:23972 |
UCSCi | uc008hfl.2 mouse |
Organism-specific databases
CTDi | 9060 |
MGIi | MGI:1330223 Papss2 |
Phylogenomic databases
eggNOGi | KOG0635 Eukaryota KOG4238 Eukaryota COG0529 LUCA COG2046 LUCA |
GeneTreei | ENSGT00390000009613 |
HOGENOMi | HOG000069045 |
HOVERGENi | HBG053503 |
InParanoidi | O88428 |
KOi | K13811 |
OMAi | QHPYIKM |
OrthoDBi | 528280at2759 |
TreeFami | TF313143 |
Enzyme and pathway databases
UniPathwayi | UPA00097 |
Reactomei | R-MMU-174362 Transport and synthesis of PAPS |
Miscellaneous databases
ChiTaRSi | Papss2 mouse |
PROi | PR:O88428 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024899 Expressed in 331 organ(s), highest expression level in intestinal epithelium |
Genevisiblei | O88428 MM |
Family and domain databases
CDDi | cd02027 APSK, 1 hit cd00517 ATPS, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
HAMAPi | MF_00065 Adenylyl_sulf_kinase, 1 hit |
InterProi | View protein in InterPro IPR002891 APS_kinase IPR025980 ATP-Sase_PUA-like_dom IPR027417 P-loop_NTPase IPR015947 PUA-like_sf IPR014729 Rossmann-like_a/b/a_fold IPR024951 Sulfurylase_cat_dom IPR002650 Sulphate_adenylyltransferase |
Pfami | View protein in Pfam PF01747 ATP-sulfurylase, 1 hit PF14306 PUA_2, 1 hit |
SUPFAMi | SSF52540 SSF52540, 1 hit SSF88697 SSF88697, 1 hit |
TIGRFAMsi | TIGR00455 apsK, 1 hit TIGR00339 sopT, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | PAPS2_MOUSE | |
Accessioni | O88428Primary (citable) accession number: O88428 Secondary accession number(s): Q5BKP4, Q9Z274 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | February 13, 2019 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways