Papss2

Functionⁱ

Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. May have a important role in skeletogenesis during postnatal growth.

Catalytic activityⁱ

ATP + sulfate = diphosphate + adenylyl sulfate.

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayⁱ: sulfate assimilation

This protein is involved in the pathway sulfate assimilation, which is part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the pathway sulfate assimilation and in Sulfur metabolism.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	92	Adenylyl sulfateBy similarity	1
Binding siteⁱ	162	Adenylyl sulfateBy similarity	1
Binding siteⁱ	198	ATP 1; via carbonyl oxygenBy similarity	1
Binding siteⁱ	559	ATP 2; via amide nitrogen and carbonyl oxygenBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	53 – 58	ATP 1By similarity	6
Nucleotide bindingⁱ	415 – 418	ATP 2By similarity	4
Nucleotide bindingⁱ	517 – 521	ATP 2By similarity	5

GO - Molecular functionⁱ

adenylylsulfate kinase activity
Source: MGI
Inferred from direct assayⁱ
- 10559207
ATP binding Source: UniProtKB-KW
nucleotidyltransferase activity Source: UniProtKB
sulfate adenylyltransferase (ATP) activity
Source: MGI
Inferred from direct assayⁱ
- 10559207

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

3'-phosphoadenosine 5'-phosphosulfate biosynthetic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
blood coagulation
Source: MGI
Inferred from mutant phenotypeⁱ
- 8835524
bone development
Source: MGI
Inferred from mutant phenotypeⁱ
- 624676
sulfate assimilation
Source: MGI
Inferred from direct assayⁱ
- 10559207

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-MMU-174362 Transport and synthesis of PAPS
UniPathwayⁱ	UPA00097

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 Short name: PAPS synthase 2 Short name: PAPSS 2 Alternative name(s): Sulfurylase kinase 2 Short name: SK 2 Short name: SK2 Including the following 2 domains: Sulfate adenylyltransferase (EC:2.7.7.4) Alternative name(s): ATP-sulfurylase Sulfate adenylate transferase Short name: SAT Adenylyl-sulfate kinase (EC:2.7.1.25) Alternative name(s): 3'-phosphoadenosine-5'-phosphosulfate synthase APS kinase Adenosine-5'-phosphosulfate 3'-phosphotransferase Adenylylsulfate 3'-phosphotransferase
Gene namesⁱ	Name:Papss2 Synonyms:Atpsk2
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 19

Organism-specific databases

MGIⁱ	MGI:1330223 Papss2

Pathology & Biotechⁱ

Involvement in diseaseⁱ

Defects in Papss2 are the cause of brachymorphism (bm), a autosomal recessive disease, which is characterized by abnormal hepatic detoxification, bleeding times and postnatal growth, such as dome-shaped skull, short thick tail, and shortened but not widened limbs. The abnormal postnatal growth has been attributed to undersulfation of cartilage proteoglycans.

Keywords - Diseaseⁱ

Disease mutation

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000105962	1 – 621	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2Add BLAST		621

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O88428
PaxDbⁱ	O88428
PeptideAtlas More...PeptideAtlasⁱ	O88428
PRIDEⁱ	O88428

PTM databases

iPTMnetⁱ	O88428
PhosphoSitePlusⁱ	O88428

Expressionⁱ

Tissue specificityⁱ

Expressed in liver, cartilage, skin and brain.

Gene expression databases

Bgeeⁱ	ENSMUSG00000024899 Expressed in 331 organ(s), highest expression level in intestinal epithelium
CleanExⁱ	MM_PAPSS2
Genevisibleⁱ	O88428 MM

Interactionⁱ

Protein-protein interaction databases

IntActⁱ	O88428, 4 interactors
Molecular INTeraction database More...MINTⁱ	O88428
STRINGⁱ	10090.ENSMUSP00000025833

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O88428
SMRⁱ	O88428
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 216	Adenylyl-sulfate kinaseCuratedAdd BLAST	216
Regionⁱ	80 – 83	Adenylyl sulfate bindingBy similarity	4
Regionⁱ	97 – 100	Adenylyl sulfate bindingBy similarity	4
Regionⁱ	123 – 124	Adenylyl sulfate bindingBy similarity	2
Regionⁱ	175 – 176	Adenylyl sulfate bindingBy similarity	2
Regionⁱ	225 – 621	Sulfate adenylyltransferaseCuratedAdd BLAST	397

Sequence similaritiesⁱ

In the N-terminal section; belongs to the APS kinase family.Curated

In the C-terminal section; belongs to the sulfate adenylyltransferase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0635 Eukaryota KOG4238 Eukaryota COG0529 LUCA COG2046 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000009613
HOGENOMⁱ	HOG000069045
HOVERGENⁱ	HBG053503
InParanoidⁱ	O88428
KEGG Orthology (KO) More...KOⁱ	K13811
OMAⁱ	QHPYIKM
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G07ZR
TreeFamⁱ	TF313143

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd02027 APSK, 1 hit cd00517 ATPS, 1 hit
Gene3Dⁱ	3.40.50.620, 1 hit
HAMAPⁱ	MF_00065 Adenylyl_sulf_kinase, 1 hit
InterProⁱ	View protein in InterPro IPR002891 APS_kinase IPR025980 ATP-Sase_PUA-like_dom IPR027417 P-loop_NTPase IPR015947 PUA-like_sf IPR014729 Rossmann-like_a/b/a_fold IPR024951 Sulfurylase_cat_dom IPR002650 Sulphate_adenylyltransferase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01747 ATP-sulfurylase, 1 hit PF14306 PUA_2, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF88697 SSF88697, 1 hit
TIGRFAMsⁱ	TIGR00455 apsK, 1 hit TIGR00339 sopT, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

O88428-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSANFKMNHK RDQQKSTNVV YQAHHVSRNK RGQVVGTRGG FRGCTVWLTG 
        60         70         80         90        100
LSGAGKTTIS FALEEYLVSH AIPCYSLDGD NVRHGLNKNL GFSAGDREEN 
       110        120        130        140        150
IRRIAEVARL FADAGLVCIT SFISPFAKDR ENARKIHESA GLPFFEIFVD 
       160        170        180        190        200
APLNICESRD VKGLYKRARA GEIKGFTGID SDYEKPETPE CVLKTNLSSV 
       210        220        230        240        250
SDCVQQVVEL LQEQNIVPHT TIKGIHELFV PENKVDQIRA EAETLPSLPI 
       260        270        280        290        300
TKLDLQWVQI LSEGWATPLK GFMREKEYLQ TLHFDTLLDG VVPRDGVINM 
       310        320        330        340        350
SIPIVLPVSA DDKARLEGCS KFALMYEGRR VALLQDPEFY EHRKEERCSR 
       360        370        380        390        400
VWGTATAKHP HIKMVMESGD WLVGGDLQVL ERIRWDDGLD QYRLTPLELK 
       410        420        430        440        450
QKCKDMNADA VFAFQLRNPV HNGHALLMQD TRRRLLERGY KHPVLLLHPL 
       460        470        480        490        500
GGWTKDDDVP LEWRMKQHAA VLEERVLDPK STIVAIFPSP MLYAGPTEVQ 
       510        520        530        540        550
WHCRCRMIAG ANFYIVGRDP AGMPHPETKK DLYEPTHGGK VLSMAPGLTS 
       560        570        580        590        600
VEIIPFRVAA YNKIKKAMDF YDPARHEEFD FISGTRMRKL AREGEDPPDG 
       610        620 
FMAPKAWKVL TDYYRSLEKT N

Length:621

Mass (Da):70,351

Last modified:July 27, 2011 - v2

Checksum:ⁱ27FD5377A79EFD61

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	5	F → S in AAC40191 (PubMed:9671738).Curated		1
Sequence conflictⁱ	290 – 294	Missing in AAC98687 (PubMed:9771708).Curated		5

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	79	G → R in bm; activity abolished. 2 Publications		1
Natural variantⁱ	109	R → K1 Publication		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF052453 mRNA Translation: AAC40191.1 AF085144 mRNA Translation: AAC98687.1 BC090997 mRNA Translation: AAH90997.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS37960.1
NCBI Reference Sequences More...RefSeqⁱ	NP_035994.2, NM_011864.3
UniGeneⁱ	Mm.203916

Genome annotation databases

Ensemblⁱ	ENSMUST00000025833; ENSMUSP00000025833; ENSMUSG00000024899
GeneIDⁱ	23972
KEGGⁱ	mmu:23972
UCSC genome browser More...UCSCⁱ	uc008hfl.2 mouse

Keywords - Coding sequence diversityⁱ

Polymorphism

Protein	Similar proteins		Species	Score	Length	Source
O88428	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2	)	HUMAN		614	UniRef90_O95340
3'-phosphoadenosine 5'-phosphosulfate synthase 2		PANTR		614
Uncharacterized protein		PANPA		614
3'-phosphoadenosine 5'-phosphosulfate synthase 2		GORGO		614
Bifunctional 3-phosphoadenosine 5-phosphosulfate synthase 2		CAMFR		630
+188

Protein	Similar proteins		Species	Score	Length	Source
O88428	Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1	)	HUMAN		624	UniRef50_O43252
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1		CAVPO		624
Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1		MOUSE		624
3'-phosphoadenosine 5'-phosphosulfate synthase 1		PANTR		700
3'-phosphoadenosine 5'-phosphosulfate synthase 1		GORGO		603
+821

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF052453 mRNA Translation: AAC40191.1 AF085144 mRNA Translation: AAC98687.1 BC090997 mRNA Translation: AAH90997.1
CCDSⁱ	CCDS37960.1
RefSeqⁱ	NP_035994.2, NM_011864.3
UniGeneⁱ	Mm.203916

3D structure databases

ProteinModelPortalⁱ	O88428
SMRⁱ	O88428
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

IntActⁱ	O88428, 4 interactors
MINTⁱ	O88428
STRINGⁱ	10090.ENSMUSP00000025833

PTM databases

iPTMnetⁱ	O88428
PhosphoSitePlusⁱ	O88428

Proteomic databases

MaxQBⁱ	O88428
PaxDbⁱ	O88428
PeptideAtlasⁱ	O88428
PRIDEⁱ	O88428

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	23972
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000025833; ENSMUSP00000025833; ENSMUSG00000024899
GeneIDⁱ	23972
KEGGⁱ	mmu:23972
UCSCⁱ	uc008hfl.2 mouse

Organism-specific databases

CTDⁱ	9060
MGIⁱ	MGI:1330223 Papss2

Phylogenomic databases

eggNOGⁱ	KOG0635 Eukaryota KOG4238 Eukaryota COG0529 LUCA COG2046 LUCA
GeneTreeⁱ	ENSGT00390000009613
HOGENOMⁱ	HOG000069045
HOVERGENⁱ	HBG053503
InParanoidⁱ	O88428
KOⁱ	K13811
OMAⁱ	QHPYIKM
OrthoDBⁱ	EOG091G07ZR
TreeFamⁱ	TF313143

Enzyme and pathway databases

UniPathwayⁱ	UPA00097
Reactomeⁱ	R-MMU-174362 Transport and synthesis of PAPS

Miscellaneous databases

ChiTaRSⁱ	Papss2 mouse
Protein Ontology More...PROⁱ	PR:O88428
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000024899 Expressed in 331 organ(s), highest expression level in intestinal epithelium
CleanExⁱ	MM_PAPSS2
Genevisibleⁱ	O88428 MM

Family and domain databases

CDDⁱ	cd02027 APSK, 1 hit cd00517 ATPS, 1 hit
Gene3Dⁱ	3.40.50.620, 1 hit
HAMAPⁱ	MF_00065 Adenylyl_sulf_kinase, 1 hit
InterProⁱ	View protein in InterPro IPR002891 APS_kinase IPR025980 ATP-Sase_PUA-like_dom IPR027417 P-loop_NTPase IPR015947 PUA-like_sf IPR014729 Rossmann-like_a/b/a_fold IPR024951 Sulfurylase_cat_dom IPR002650 Sulphate_adenylyltransferase
Pfamⁱ	View protein in Pfam PF01747 ATP-sulfurylase, 1 hit PF14306 PUA_2, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF88697 SSF88697, 1 hit
TIGRFAMsⁱ	TIGR00455 apsK, 1 hit TIGR00339 sopT, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PAPS2_MOUSE
Accessionⁱ	O88428Primary (citable) accession number: O88428 Secondary accession number(s): Q5BKP4, Q9Z274
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
	Last sequence update:	July 27, 2011
	Last modified:	November 7, 2018
	This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways

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Supporting data

UniProtKB - O88428 (PAPS2_MOUSE)

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Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: sulfate assimilation

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Keywords - Diseasei

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: sulfate assimilation

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Pathology & Biotechⁱ

Keywords - Diseaseⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ