UniProtKB - O88387 (FGD4_RAT)
Protein
FYVE, RhoGEF and PH domain-containing protein 4
Gene
Fgd4
Organism
Rattus norvegicus (Rat)
Status
Functioni
Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Plays a role in regulating the actin cytoskeleton and cell shape. Activates MAPK8.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 559 – 619 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
GO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: RGD
- metal ion binding Source: UniProtKB-KW
- Rho guanyl-nucleotide exchange factor activity Source: InterPro
GO - Biological processi
- activation of JUN kinase activity Source: MGI
- regulation of cell shape Source: RGD
- regulation of Rho protein signal transduction Source: InterPro
Keywordsi
Molecular function | Actin-binding, Guanine-nucleotide releasing factor |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-193648 NRAGE signals death through JNK R-RNO-194840 Rho GTPase cycle R-RNO-416482 G alpha (12/13) signalling events |
Names & Taxonomyi
Protein namesi | Recommended name: FYVE, RhoGEF and PH domain-containing protein 4Alternative name(s): Actin filament-binding protein frabin FGD1-related F-actin-binding protein |
Gene namesi | Name:Fgd4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 708357 Fgd4 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- filopodium 1 Publication
Note: Concentrated in filopodia and poorly detected at lamellipodia. Binds along the sides of actin fibers.
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-KW
- filopodium Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell projection, Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080949 | 1 – 766 | FYVE, RhoGEF and PH domain-containing protein 4Add BLAST | 766 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 702 | PhosphoserineBy similarity | 1 | |
Modified residuei | 716 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O88387 |
PRIDEi | O88387 |
Expressioni
Tissue specificityi
Detected in brain, lung, liver, skeletal muscle, kidney, testis and cultured hippocampal neurons.1 Publication
Interactioni
Subunit structurei
Homooligomer.
GO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: RGD
- Rho guanyl-nucleotide exchange factor activity Source: InterPro
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000002491 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 206 – 393 | DHPROSITE-ProRule annotationAdd BLAST | 188 | |
Domaini | 422 – 521 | PH 1PROSITE-ProRule annotationAdd BLAST | 100 | |
Domaini | 643 – 740 | PH 2PROSITE-ProRule annotationAdd BLAST | 98 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 150 | Actin filament-bindingAdd BLAST | 150 |
Domaini
The part of the protein spanning the actin filament-binding domain together with the DH domain and the first PH domain is necessary and sufficient for microspike formation. Activation of MAPK8 requires the presence of all domains with the exception of the actin filament-binding domain.
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 559 – 619 | FYVE-typePROSITE-ProRule annotationAdd BLAST | 61 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG4424 Eukaryota ENOG410XRXV LUCA |
HOGENOMi | HOG000220866 |
InParanoidi | O88387 |
KOi | K05723 |
OrthoDBi | 652460at2759 |
PhylomeDBi | O88387 |
Family and domain databases
CDDi | cd15791 PH1_FDG4, 1 hit cd13236 PH2_FGD1-4, 1 hit cd00160 RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 2 hits 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR035899 DBL_dom_sf IPR000219 DH-domain IPR037742 FDG4_N_PH IPR035941 FGD1-4_PH2 IPR011993 PH-like_dom_sf IPR001849 PH_domain IPR000306 Znf_FYVE IPR017455 Znf_FYVE-rel IPR013083 Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363 FYVE, 1 hit PF00169 PH, 2 hits PF00621 RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00064 FYVE, 1 hit SM00233 PH, 2 hits SM00325 RhoGEF, 1 hit |
SUPFAMi | SSF48065 SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS50010 DH_2, 1 hit PS50003 PH_DOMAIN, 2 hits PS50178 ZF_FYVE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
O88387-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEESNPAPTS CASKGKHSKV SDLISHFEGG SVLSSYTDVQ KDSTMNLNIP
60 70 80 90 100
QTPRQHGLTS TTPQKLPSHK SPQKQEKDSD QNQGQHGCLA NGVAAAQSQM
110 120 130 140 150
ECETEKEAAL SPETDTQTAA ASPDAHVLNG VRNETTTDSA SSVTNSHDEN
160 170 180 190 200
ACDSSCRTQG TDLGLPSKEG EPVIEAELQE RENGLSTEGL NPLDQHHEVK
210 220 230 240 250
ETNEQKLHKI ATELLLTERA YVSRLNLLDQ VFYCKLLEEA NRGSFPAEMV
260 270 280 290 300
NKIFSNISSI NAFHSKFLLP ELEKRMQEWE TTPRIGDILQ KLAPFLKMYG
310 320 330 340 350
EYVKGFDNAV ELVKNMTERV PQFKSVTEEI QKQKICGSLT LQHHMLEPIQ
360 370 380 390 400
RIPRYEMLLK DYLKKLSPDA PDWNDAKKSL EIISTAASHS NSAIRKMENL
410 420 430 440 450
KKLLEIYEML GEEEDIVNPS NELIKEGQIL KLAARNTSAQ ERYLFLFNNM
460 470 480 490 500
LLYCVPRFSL VGSKFTVRTR VGIDGMKIVE THNEEYPHTF QVSGKERTLE
510 520 530 540 550
LQASSEQDKE EWIKALQESI DAFHQRHETF RNAIAKENDI PLEVSTAELG
560 570 580 590 600
KRAPRWIRDN EVTMCMKCKE SFNALTRRRH HCRACGHVVC WKCSDYKAQL
610 620 630 640 650
EYDGGRLNKV CKDCYQIMSG FAESEEKKRR GILEIESAEV SGNSEVCSFL
660 670 680 690 700
QYMEKSKPWQ KIWCVIPKQD PLVLYMYGAP QDVRAQATIP LLGYIVDDMP
710 720 730 740 750
KSADLPHSFK LTQSKSVHSF AADSEELKQK WLKIILLAVT GETPDGPSEH
760
LDTLDNLPGP KEKSEC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K8X0 | A0A0G2K8X0_RAT | FYVE, RhoGEF and PH domain-containi... | Fgd4 | 764 | Annotation score: | ||
A0A0G2K2S8 | A0A0G2K2S8_RAT | FYVE, RhoGEF and PH domain-containi... | Fgd4 | 764 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF038388 mRNA Translation: AAC27698.1 |
RefSeqi | NP_640356.1, NM_139263.1 |
Genome annotation databases
GeneIDi | 246174 |
KEGGi | rno:246174 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF038388 mRNA Translation: AAC27698.1 |
RefSeqi | NP_640356.1, NM_139263.1 |
3D structure databases
SMRi | O88387 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000002491 |
Proteomic databases
PaxDbi | O88387 |
PRIDEi | O88387 |
Genome annotation databases
GeneIDi | 246174 |
KEGGi | rno:246174 |
Organism-specific databases
CTDi | 121512 |
RGDi | 708357 Fgd4 |
Phylogenomic databases
eggNOGi | KOG4424 Eukaryota ENOG410XRXV LUCA |
HOGENOMi | HOG000220866 |
InParanoidi | O88387 |
KOi | K05723 |
OrthoDBi | 652460at2759 |
PhylomeDBi | O88387 |
Enzyme and pathway databases
Reactomei | R-RNO-193648 NRAGE signals death through JNK R-RNO-194840 Rho GTPase cycle R-RNO-416482 G alpha (12/13) signalling events |
Miscellaneous databases
PROi | PR:O88387 |
Family and domain databases
CDDi | cd15791 PH1_FDG4, 1 hit cd13236 PH2_FGD1-4, 1 hit cd00160 RhoGEF, 1 hit |
Gene3Di | 1.20.900.10, 1 hit 2.30.29.30, 2 hits 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR035899 DBL_dom_sf IPR000219 DH-domain IPR037742 FDG4_N_PH IPR035941 FGD1-4_PH2 IPR011993 PH-like_dom_sf IPR001849 PH_domain IPR000306 Znf_FYVE IPR017455 Znf_FYVE-rel IPR013083 Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF01363 FYVE, 1 hit PF00169 PH, 2 hits PF00621 RhoGEF, 1 hit |
SMARTi | View protein in SMART SM00064 FYVE, 1 hit SM00233 PH, 2 hits SM00325 RhoGEF, 1 hit |
SUPFAMi | SSF48065 SSF48065, 1 hit |
PROSITEi | View protein in PROSITE PS50010 DH_2, 1 hit PS50003 PH_DOMAIN, 2 hits PS50178 ZF_FYVE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FGD4_RAT | |
Accessioni | O88387Primary (citable) accession number: O88387 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2005 |
Last sequence update: | November 1, 1998 | |
Last modified: | November 13, 2019 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |