UniProtKB - O88382 (MAGI2_RAT)
Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2
Magi2
Functioni
GO - Molecular functioni
- activin receptor binding Source: RGD
- beta-1 adrenergic receptor binding Source: UniProtKB
- kinesin binding Source: RGD
- phosphatase binding Source: UniProtKB
- protein-containing complex binding Source: RGD
- signaling receptor complex adaptor activity Source: UniProtKB
- SMAD binding Source: UniProtKB
- structural constituent of postsynaptic density Source: SynGO
- structural constituent of postsynaptic specialization Source: SynGO
- type II activin receptor binding Source: UniProtKB
GO - Biological processi
- cellular response to nerve growth factor stimulus Source: UniProtKB
- glomerular filtration Source: UniProtKB
- glomerular visceral epithelial cell development Source: UniProtKB
- mitotic cell cycle arrest Source: UniProtKB
- negative regulation of activin receptor signaling pathway Source: UniProtKB
- negative regulation of cell migration Source: UniProtKB
- negative regulation of cell population proliferation Source: UniProtKB
- negative regulation of protein kinase B signaling Source: UniProtKB
- nerve growth factor signaling pathway Source: UniProtKB
- neuroligin clustering involved in postsynaptic membrane assembly Source: RGD
- positive regulation of neuron projection development Source: UniProtKB
- positive regulation of phosphoprotein phosphatase activity Source: UniProtKB
- positive regulation of receptor internalization Source: UniProtKB
- positive regulation of synaptic vesicle clustering Source: RGD
- receptor clustering Source: RGD
- regulation of neurotransmitter receptor localization to postsynaptic specialization membrane Source: SynGO
- regulation of synaptic membrane adhesion Source: SynGO
- signal transduction Source: RGD
- SMAD protein signal transduction Source: UniProtKB
Keywordsi
Biological process | Neurogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2Alternative name(s): Atrophin-1-interacting protein 1 Short name: AIP-1 Membrane-associated guanylate kinase inverted 2 Short name: MAGI-2 Synaptic-scaffolding molecule Short name: S-SCAM |
Gene namesi | Name:Magi2 Synonyms:Acvrinp1, Aip1, Sscam |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 621855, Magi2 |
Subcellular locationi
Endosome
Cytoplasm and Cytosol
Plasma membrane
Other locations
Note: Membrane-associated in synaptosomes. Localized diffusely in the cytoplasm before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation.
Endosome
- late endosome Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- extrinsic component of postsynaptic membrane Source: SynGO
- plasma membrane Source: UniProtKB
Other locations
- bicellular tight junction Source: UniProtKB
- cell-cell junction Source: GO_Central
- cytoplasm Source: UniProtKB
- dendrite Source: UniProtKB
- GABA-ergic synapse Source: SynGO
- glutamatergic synapse Source: SynGO
- perinuclear region of cytoplasm Source: UniProtKB
- postsynaptic density Source: UniProtKB
- protein-containing complex Source: UniProtKB
- slit diaphragm Source: UniProtKB
- synapse Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell membrane, Cytoplasm, Endosome, Membrane, Synapse, SynaptosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094588 | 1 – 1277 | Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 2Add BLAST | 1277 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 362 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 686 | PhosphoserineCombined sources | 1 | |
Modified residuei | 827 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 884 | PhosphoserineCombined sources | 1 | |
Modified residuei | 885 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O88382 |
PRIDEi | O88382 |
PTM databases
iPTMneti | O88382 |
PhosphoSitePlusi | O88382 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Interacts (via its WW domains) with DRPLA (By similarity).
Interacts with CTNNB1, ACVR2A, SMAD2 and SMAD3 (By similarity).
Part of a complex consisting of AIP1, ACVR2A, ACVR1B and SMAD3 (By similarity). May interact with HTR2A and IGSF9 (By similarity).
Interacts with HTR4 (By similarity).
Interacts (via guanylate kinase domain) with DLGAP1 (PubMed:9694864).
Interacts (via PDZ domains) with GRIN2A, GRID2 and NLGN1 (PubMed:9694864, PubMed:12589829).
Interacts with CTNND2 (PubMed:10080919).
Interacts with MAGUIN-1 (PubMed:10207009).
Interacts (via its second PDZ domain) with PTEN (via unphosphorylated C-terminus); this interaction diminishes the degradation rate of PTEN (PubMed:15951562).
Found in a complex, at least composed of KIDINS220, MAGI2, NTRK1 and RAPGEF2; the complex is mainly formed at late endosomes in a NGF-dependent manner (PubMed:17724123).
Interacts with RAPGEF2; the interaction occurs before or after nerve growth factor (NGF) stimulation (PubMed:10548487). Isoform 1 interacts (via PDZ domain) with KIDINS220 isoform 2 (via C-terminal domain) (PubMed:17724123).
Interacts with DDN (PubMed:16751601, PubMed:16464232).
Identified in a complex with ACTN4, CASK, IQGAP1, NPHS1, SPTAN1 and SPTBN1 (PubMed:15994232).
Interacts with DLL1 (By similarity).
Found in a complex with IGSF9B and NLGN2; the interaction with IGSF9B is mediated via the PDZ 5 and PDZ 6 domains, while the interaction with NLGN2 is mediated via the WW1, WW2 and PDZ2 domains (PubMed:23751499).
By similarity11 PublicationsBinary interactionsi
Hide detailsO88382
GO - Molecular functioni
- activin receptor binding Source: RGD
- beta-1 adrenergic receptor binding Source: UniProtKB
- kinesin binding Source: RGD
- phosphatase binding Source: UniProtKB
- SMAD binding Source: UniProtKB
- type II activin receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 250241, 7 interactors |
CORUMi | O88382 |
IntActi | O88382, 28 interactors |
MINTi | O88382 |
STRINGi | 10116.ENSRNOP00000059912 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 101 | PDZPROSITE-ProRule annotationAdd BLAST | 85 | |
Domaini | 109 – 283 | Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST | 175 | |
Domaini | 302 – 335 | WW 1PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 348 – 381 | WW 2PROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 426 – 510 | PDZ 1PROSITE-ProRule annotationAdd BLAST | 85 | |
Domaini | 605 – 683 | PDZ 2PROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 778 – 860 | PDZ 3PROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 920 – 1010 | PDZ 4PROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 1141 – 1223 | PDZ 5PROSITE-ProRule annotationAdd BLAST | 83 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 302 – 381 | Interaction with DDNBy similarityAdd BLAST | 80 |
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3209, Eukaryota |
InParanoidi | O88382 |
OMAi | RNGRMRX |
OrthoDBi | 284488at2759 |
PhylomeDBi | O88382 |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
Gene3Di | 2.30.42.10, 6 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR030036, MAGI2 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR10316:SF27, PTHR10316:SF27, 1 hit |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 6 hits PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 6 hits SM00456, WW, 2 hits |
SUPFAMi | SSF50156, SSF50156, 6 hits SSF51045, SSF51045, 2 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 6 hits PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSKSLKKKSH WTSKVHESVI GRNPEGQLGF ELKGGAENGQ FPYLGEVKPG
60 70 80 90 100
KVAYESGSKL VSEELLLEVN ETPVAGLTIR DVLAVIKHCK DPLRLKCVKQ
110 120 130 140 150
GGIVDKDLRH YLNLRFQKGS VDHELQQIIR DNLYLRTVPC TTRPHKEGEV
160 170 180 190 200
PGVDYIFITV EDFMELEKSG ALLESGTYED NYYGTPKPPA EPAPLLLNVT
210 220 230 240 250
DQILPGATPS AEGKRKRNKS VTNMEKASIE PPEEEEEERP VVNGNGVVIT
260 270 280 290 300
PESSEHEDKS AGASGETPSQ PYPAPVYSQP EELKDQMDDT KSTKPEENED
310 320 330 340 350
SDPLPDNWEM AYTEKGEVYF IDHNTKTTSW LDPRLAKKAK PAEECKENEL
360 370 380 390 400
PYGWEKIDDP IYGTYYVDHI NRRTQFENPV LEAKRKLQQH NMPHTELGTK
410 420 430 440 450
PLQAPGFREK PLFTRDASQL KGTFLSTTLK KSNMGFGFTI IGGDEPDEFL
460 470 480 490 500
QVKSVIPDGP AAQDGKMETG DVIVYINEVC VLGHTHADVV KLFQSVPIGQ
510 520 530 540 550
SVNLVLCRGY PLPFDPEDPA NSMVPPLAIM ERPPPVMVNG RHNYETYLEY
560 570 580 590 600
ISRTSQSVPD ITDRPPHSLH SMPADGQLDG TYPPPVHDDN VSVASSGATQ
610 620 630 640 650
AELMTLTIVK GAKGFGFTIA DSPTGQRVKQ ILDIQGCPGL CEGDLIVEIN
660 670 680 690 700
QQNVQNLSHT EVVDILKDCP VGSETSLIIH RGGFFSPWKT PKPMVDRWEN
710 720 730 740 750
QGSPQTSLSA PAVPQSLPFP PALHRSSFPD STEAFDPRKP DPYELYEKSR
760 770 780 790 800
AIYESRQQVP PRTSFRMDSS GPDYKELDVH LRRMESGFGF RILGGDEPGQ
810 820 830 840 850
PILIGAVIAM GSADRDGRLH PGDELVYVDG IPVAGKTHRY VIDLMHHAAR
860 870 880 890 900
NGQVNLTVRR KVLCGGEPCP ENGRSPGSVS THHSSPRSDY ATYANSNHAA
910 920 930 940 950
PSNNASPPEG FASHSLQTSD VIIHRKENEG FGFVIISSLN RPESGATITV
960 970 980 990 1000
PHKIGRIIDG SPADRCAKLK VGDRILAVNG QSIINMPHAD IVKLIKDAGL
1010 1020 1030 1040 1050
SVTLRIIPQE ELNNPTSAPS SEKQSPMAQQ HSPLAQQHSP LAQPSPATPN
1060 1070 1080 1090 1100
SPVAQPAPPQ PLQLQGHENS YRSEVKARQD VKPDIRQPPF TDYRQPPLDY
1110 1120 1130 1140 1150
RQPPGGDYSQ PSPLDYRQHS PDTRQYPLSD YRQPQDFDYF TVDMEKGAKG
1160 1170 1180 1190 1200
FGFSIRGGRE YKMDLYVLRL AEDGPAIRNG RMRVGDQIIE INGESTRDMT
1210 1220 1230 1240 1250
HARAIELIKS GGRRVRLLLK RGTGQVPEYG MVPSSLSMCM KSDKHGSPYF
1260 1270
YLLGHPKDTT NPTPGALPLP PPQACRK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3ZY03 | D3ZY03_RAT | Membrane-associated guanylate kinas... | Magi2 | 1,138 | Annotation score: | ||
A0A0G2JXR9 | A0A0G2JXR9_RAT | Membrane-associated guanylate kinas... | Magi2 | 1,082 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 645 | L → F in AAD31015 (PubMed:10644767).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_008439 | 1 – 223 | Missing in isoform 3. CuratedAdd BLAST | 223 | |
Alternative sequenceiVSP_008438 | 1 – 163 | Missing in isoform 2. 1 PublicationAdd BLAST | 163 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF034863 mRNA Translation: AAC31124.1 AF130819 mRNA Translation: AAD31015.1 |
PIRi | T14152 |
RefSeqi | NP_446073.1, NM_053621.1 [O88382-1] |
Genome annotation databases
GeneIDi | 113970 |
KEGGi | rno:113970 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF034863 mRNA Translation: AAC31124.1 AF130819 mRNA Translation: AAD31015.1 |
PIRi | T14152 |
RefSeqi | NP_446073.1, NM_053621.1 [O88382-1] |
3D structure databases
SMRi | O88382 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 250241, 7 interactors |
CORUMi | O88382 |
IntActi | O88382, 28 interactors |
MINTi | O88382 |
STRINGi | 10116.ENSRNOP00000059912 |
PTM databases
iPTMneti | O88382 |
PhosphoSitePlusi | O88382 |
Proteomic databases
PaxDbi | O88382 |
PRIDEi | O88382 |
Genome annotation databases
GeneIDi | 113970 |
KEGGi | rno:113970 |
Organism-specific databases
CTDi | 9863 |
RGDi | 621855, Magi2 |
Phylogenomic databases
eggNOGi | KOG3209, Eukaryota |
InParanoidi | O88382 |
OMAi | RNGRMRX |
OrthoDBi | 284488at2759 |
PhylomeDBi | O88382 |
Miscellaneous databases
PROi | PR:O88382 |
Family and domain databases
CDDi | cd00201, WW, 2 hits |
Gene3Di | 2.30.42.10, 6 hits |
InterProi | View protein in InterPro IPR008145, GK/Ca_channel_bsu IPR008144, Guanylate_kin-like_dom IPR020590, Guanylate_kinase_CS IPR030036, MAGI2 IPR027417, P-loop_NTPase IPR001478, PDZ IPR036034, PDZ_sf IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR10316:SF27, PTHR10316:SF27, 1 hit |
Pfami | View protein in Pfam PF00625, Guanylate_kin, 1 hit PF00595, PDZ, 6 hits PF00397, WW, 1 hit |
SMARTi | View protein in SMART SM00072, GuKc, 1 hit SM00228, PDZ, 6 hits SM00456, WW, 2 hits |
SUPFAMi | SSF50156, SSF50156, 6 hits SSF51045, SSF51045, 2 hits SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00856, GUANYLATE_KINASE_1, 1 hit PS50052, GUANYLATE_KINASE_2, 1 hit PS50106, PDZ, 6 hits PS01159, WW_DOMAIN_1, 2 hits PS50020, WW_DOMAIN_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MAGI2_RAT | |
Accessioni | O88382Primary (citable) accession number: O88382 Secondary accession number(s): Q9R271 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2003 |
Last sequence update: | November 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families