UniProtKB - O88342 (WDR1_MOUSE)
Protein
WD repeat-containing protein 1
Gene
Wdr1
Organism
Mus musculus (Mouse)
Status
Functioni
Induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins (By similarity). Enhances cofilin-mediated actin severing (PubMed:25915128). Involved in cytokinesis. Involved in chemotactic cell migration by restricting lamellipodial membrane protrusions (By similarity). Involved in myocardium sarcomere organization. Required for cardiomyocyte growth at the postnatal and maintenance at the adult stage (PubMed:24840128). Involved in neutrophil actin dynamics and migration. Involved in megakaryocyte maturation and platelet shedding (PubMed:17515402). Required for the establishment of planar cell polarity (PCP) during follicular epithelium development and for cell shape changes during PCP; the function seems to implicate cooperation with CFL1 and/or DSTN/ADF. Involved in the generation/maintenance of cortical tension (PubMed:25915128). Involved in assembly and maintenance of epithelial apical cell junctions and plays a role in the organization of the perijunctional actomyosin belt (By similarity).By similarity2 Publications
GO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: UniProtKB
GO - Biological processi
- actin cytoskeleton organization Source: MGI
- actin filament depolymerization Source: GO_Central
- actin filament fragmentation Source: UniProtKB
- apical junction assembly Source: MGI
- cortical cytoskeleton organization Source: UniProtKB
- establishment of planar polarity of follicular epithelium Source: UniProtKB
- locomotion Source: GO_Central
- maintenance of epithelial cell apical/basal polarity Source: MGI
- neutrophil mediated immunity Source: UniProtKB
- neutrophil migration Source: UniProtKB
- platelet formation Source: UniProtKB
- positive regulation of actin filament depolymerization Source: MGI
- regulation of actin filament depolymerization Source: MGI
- regulation of cell shape Source: UniProtKB
- regulation of oligodendrocyte differentiation Source: MGI
- regulation of ventricular cardiac muscle cell membrane repolarization Source: MGI
- sarcomere organization Source: MGI
- sensory perception of sound Source: MGI
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: WD repeat-containing protein 1Alternative name(s): Actin-interacting protein 1 Short name: AIP1 |
Gene namesi | Name:Wdr1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1337100, Wdr1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton By similarity
Other locations
- podosome By similarity
Cytoskeleton
- actin cytoskeleton Source: MGI
- actin filament Source: Ensembl
- cortical actin cytoskeleton Source: GO_Central
- podosome Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cell junction Source: MGI
- cell projection Source: UniProtKB-KW
- cell-cell junction Source: MGI
- myelin sheath Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Cell projection, Cytoplasm, CytoskeletonPathology & Biotechi
Disruption phenotypei
Embryonic lethal. Partial deficiencies disrupt megakaryocyte maturation, platelet shedding and provoke neutrophilic autoinflammatory disease.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000051342 | 1 – 606 | WD repeat-containing protein 1Add BLAST | 606 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 28 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 81 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 95 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 115 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 238 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 480 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3680 non-CPTAC-3955 |
EPDi | O88342 |
jPOSTi | O88342 |
PaxDbi | O88342 |
PeptideAtlasi | O88342 |
PRIDEi | O88342 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00314748 O88342 |
UCD-2DPAGEi | O88342 |
PTM databases
iPTMneti | O88342 |
PhosphoSitePlusi | O88342 |
SwissPalmi | O88342 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000005103, Expressed in lymph node and 313 other tissues |
Genevisiblei | O88342, MM |
Interactioni
GO - Molecular functioni
- actin binding Source: MGI
- actin filament binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 204554, 24 interactors |
IntActi | O88342, 13 interactors |
MINTi | O88342 |
STRINGi | 10090.ENSMUSP00000005234 |
Miscellaneous databases
RNActi | O88342, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 4 – 45 | WD 1Add BLAST | 42 | |
Repeati | 48 – 87 | WD 2Add BLAST | 40 | |
Repeati | 93 – 135 | WD 3Add BLAST | 43 | |
Repeati | 138 – 176 | WD 4Add BLAST | 39 | |
Repeati | 180 – 218 | WD 5Add BLAST | 39 | |
Repeati | 224 – 263 | WD 6Add BLAST | 40 | |
Repeati | 270 – 306 | WD 7Add BLAST | 37 | |
Repeati | 311 – 351 | WD 8Add BLAST | 41 | |
Repeati | 358 – 408 | WD 9Add BLAST | 51 | |
Repeati | 432 – 474 | WD 10Add BLAST | 43 | |
Repeati | 480 – 518 | WD 11Add BLAST | 39 | |
Repeati | 523 – 561 | WD 12Add BLAST | 39 | |
Repeati | 566 – 604 | WD 13Add BLAST | 39 |
Sequence similaritiesi
Belongs to the WD repeat AIP1 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0318, Eukaryota |
GeneTreei | ENSGT00390000009416 |
HOGENOMi | CLU_015246_3_0_1 |
InParanoidi | O88342 |
OMAi | TNPAKHT |
OrthoDBi | 325552at2759 |
PhylomeDBi | O88342 |
TreeFami | TF300821 |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR011045, N2O_reductase_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 11 hits |
SUPFAMi | SSF50974, SSF50974, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O88342-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPYEIKKVFA SLPQVERGVS KILGGDPKGD HFLYTNGKCV ILRNIDNPAI
60 70 80 90 100
ADIYTEHAHQ VVVAKYAPSG FYIASGDISG KLRIWDTTQK EHLLKYEYQP
110 120 130 140 150
FAGKIKDIAW TEDSKRIAVV GEGREKFGAV FLWDTGSSVG EITGHNKVIN
160 170 180 190 200
SVDIKQTRPY RLATGSDDNC AAFFEGPPFK FKFTIGDHSR FVNCVRFSPD
210 220 230 240 250
GNRFATASAD GQIFIYDGKT GEKVCALGES KAHDGGIYAI SWSPDSTHLL
260 270 280 290 300
SASGDKTSKI WDVNVNSVVS TFPMGSNVLD QQLGCLWQKD HLLSISLSGY
310 320 330 340 350
INYLDKNNPS KPLRVIKGHS KSIQCLTVHR NGGKSYIYSG SHDGHINYWD
360 370 380 390 400
SETGENDSFS GKGHTNQVSR MTVNESEQLV SCSMDDTVRY TNLTLRDYSG
410 420 430 440 450
QGVVKLDVQP KCVAVGPGGY TVVVCIGQIV LLKDQKKCFS IDNPGYEPEV
460 470 480 490 500
VAVHPGGDTV AVGGTDGNVR VYSILASTLK DEGKLLEAKG PVTDVAYSHD
510 520 530 540 550
GAFLAVCDAS KVVTVFSVAD GYSENNVFYG HHAKIVCLAW SPDNEHFASG
560 570 580 590 600
GMDMMVYVWT LSDPETKVKI QDAHRLHHVS SLAWLDEHTL VTTSHDASVK
EWTITY
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0J9YU05 | A0A0J9YU05_MOUSE | WD repeat-containing protein 1 | Wdr1 | 333 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF020055 mRNA Translation: AAD05043.1 |
CCDSi | CCDS51485.1 |
RefSeqi | NP_035845.1, NM_011715.2 |
Genome annotation databases
Ensembli | ENSMUST00000005234; ENSMUSP00000005234; ENSMUSG00000005103 |
GeneIDi | 22388 |
KEGGi | mmu:22388 |
UCSCi | uc033ije.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF020055 mRNA Translation: AAD05043.1 |
CCDSi | CCDS51485.1 |
RefSeqi | NP_035845.1, NM_011715.2 |
3D structure databases
SMRi | O88342 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 204554, 24 interactors |
IntActi | O88342, 13 interactors |
MINTi | O88342 |
STRINGi | 10090.ENSMUSP00000005234 |
PTM databases
iPTMneti | O88342 |
PhosphoSitePlusi | O88342 |
SwissPalmi | O88342 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00314748 O88342 |
UCD-2DPAGEi | O88342 |
Proteomic databases
CPTACi | non-CPTAC-3680 non-CPTAC-3955 |
EPDi | O88342 |
jPOSTi | O88342 |
PaxDbi | O88342 |
PeptideAtlasi | O88342 |
PRIDEi | O88342 |
Protocols and materials databases
Antibodypediai | 22867, 152 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000005234; ENSMUSP00000005234; ENSMUSG00000005103 |
GeneIDi | 22388 |
KEGGi | mmu:22388 |
UCSCi | uc033ije.1, mouse |
Organism-specific databases
CTDi | 9948 |
MGIi | MGI:1337100, Wdr1 |
Phylogenomic databases
eggNOGi | KOG0318, Eukaryota |
GeneTreei | ENSGT00390000009416 |
HOGENOMi | CLU_015246_3_0_1 |
InParanoidi | O88342 |
OMAi | TNPAKHT |
OrthoDBi | 325552at2759 |
PhylomeDBi | O88342 |
TreeFami | TF300821 |
Enzyme and pathway databases
Reactomei | R-MMU-114608, Platelet degranulation |
Miscellaneous databases
BioGRID-ORCSi | 22388, 5 hits in 16 CRISPR screens |
ChiTaRSi | Wdr1, mouse |
PROi | PR:O88342 |
RNActi | O88342, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005103, Expressed in lymph node and 313 other tissues |
Genevisiblei | O88342, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR011045, N2O_reductase_N IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 11 hits |
SUPFAMi | SSF50974, SSF50974, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 5 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | WDR1_MOUSE | |
Accessioni | O88342Primary (citable) accession number: O88342 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families