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Protein

Epsin-1

Gene

Epn1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Modifies membrane curvature and facilitates the formation of clathrin-coated invaginations. Regulates receptor-mediated endocytosis.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8Phosphatidylinositol lipid headgroup1
Binding sitei11Phosphatidylinositol lipid headgroup1
Binding sitei25Phosphatidylinositol lipid headgroup1
Binding sitei30Phosphatidylinositol lipid headgroup1
Binding sitei63Phosphatidylinositol lipid headgroup1
Binding sitei73Phosphatidylinositol lipid headgroup1

GO - Molecular functioni

  • clathrin adaptor activity Source: CAFA
  • ion channel binding Source: RGD
  • lipid binding Source: UniProtKB-KW
  • transcription factor binding Source: RGD

GO - Biological processi

  • endocytosis Source: RGD
  • positive regulation of clathrin coat assembly Source: CAFA

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-1
Alternative name(s):
EPS-15-interacting protein 1
Gene namesi
Name:Epn1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi619772 Epn1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi6L → E, H or Q: Reduces lipid binding. 1 Publication1
Mutagenesisi8R → A: Strongly reduces lipid binding. 1 Publication1
Mutagenesisi63R → L: Strongly reduces lipid binding. Abolishes lipid binding; when associated with L-73. 2 Publications1
Mutagenesisi72R → A: Abolishes ZNF145 binding. 1 Publication1
Mutagenesisi73H → L: Abolishes lipid binding; when associated with L-63. 1 Publication1
Mutagenesisi76K → A: Strongly reduces lipid binding. 1 Publication1
Mutagenesisi190L → A: Abolishes mono-ubiquitination. 1 Publication1
Mutagenesisi257 – 259LMD → AAA: Strongly reduces clathrin binding. 1 Publication3
Mutagenesisi260 – 263LADV → AAAA: Strongly reduces clathrin binding. 1 Publication4

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745151 – 575Epsin-1Add BLAST575

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei382PhosphoserineBy similarity1
Modified residuei418PhosphoserineBy similarity1
Modified residuei419PhosphoserineBy similarity1
Modified residuei420PhosphothreonineBy similarity1
Modified residuei434PhosphoserineBy similarity1
Modified residuei446PhosphoserineBy similarity1
Modified residuei453PhosphoserineCombined sources1
Modified residuei459PhosphothreonineBy similarity1
Modified residuei463PhosphothreonineBy similarity1
Modified residuei469PhosphothreonineCombined sources1
Modified residuei472PhosphoserineBy similarity1
Modified residuei493PhosphothreonineBy similarity1
Modified residuei533Omega-N-methylarginineBy similarity1

Post-translational modificationi

Ubiquitinated.1 Publication
Phosphorylated on serine and/or threonine residues in mitotic cells. Phosphorylation reduces interaction with REPS2, AP-2 and the membrane fraction. Depolarization of synaptosomes results in dephosphorylation.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO88339
PeptideAtlasiO88339
PRIDEiO88339

PTM databases

iPTMnetiO88339
PhosphoSitePlusiO88339

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Gene expression databases

GenevisibleiO88339 RN

Interactioni

Subunit structurei

Monomer. Binds REPS2 and ITSN1 (By similarity). Binds EPS15, clathrin, ZBTB16/ZNF145, AP2A1 and AP2A2. Binds ubiquitinated proteins. Interacts with RALBP1 in a complex also containing NUMB and TFAP2A during interphase and mitosis. Interacts with AP2B1. Interacts with UBQLN2 (By similarity).By similarity10 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi250724, 15 interactors
CORUMiO88339
DIPiDIP-40738N
ELMiO88339
IntActiO88339, 4 interactors
MINTiO88339
STRINGi10116.ENSRNOP00000021286

Structurei

Secondary structure

1575
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00251
ProteinModelPortaliO88339
SMRiO88339
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO88339

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini12 – 144ENTHPROSITE-ProRule annotationAdd BLAST133
Domaini183 – 202UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini208 – 227UIM 2PROSITE-ProRule annotationAdd BLAST20
Domaini233 – 252UIM 3PROSITE-ProRule annotationAdd BLAST20
Repeati274 – 27613
Repeati294 – 29623
Repeati306 – 30833
Repeati319 – 32143
Repeati332 – 33453
Repeati349 – 35163
Repeati367 – 36973
Repeati377 – 37983
Repeati501 – 50313
Repeati517 – 51923
Repeati571 – 57333

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni274 – 3798 X 3 AA repeats of D-P-WAdd BLAST106
Regioni501 – 5733 X 3 AA repeats of N-P-FAdd BLAST73

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi401 – 410[DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif10

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi267 – 572Ala/Gly/Pro-richAdd BLAST306

Domaini

The NPF repeat domain is involved in EPS15 binding.
The DPW repeat domain is involved in AP2A2 and clathrin binding.
The [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif mediates interaction the AP-2 complex subunit AP2B1.By similarity

Sequence similaritiesi

Belongs to the epsin family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2056 Eukaryota
ENOG410XSM0 LUCA
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiO88339
KOiK12471
OrthoDBiEOG091G0L45
PhylomeDBiO88339

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
PF02809 UIM, 2 hits
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 3 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 3 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O88339-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSTSSLRRQM KNIVHNYSEA EIKVREATSN DPWGPSSSLM SEIADLTYNV
60 70 80 90 100
VAFSEIMSMI WKRLNDHGKN WRHVYKAMTL MEYLIKTGSE RVSQQCKENM
110 120 130 140 150
YAVQTLKDFQ YVDRDGKDQG VNVREKAKQL VALLRDEDRL REERAHALKT
160 170 180 190 200
KEKLAQTATA SSAAVGSGPP PEAEQAWPQS SGEEELQLQL ALAMSKEEAD
210 220 230 240 250
QPPSCGPEDD VQLQLALSLS REEHDKEERI RRGDDLRLQM AIEESKRETG
260 270 280 290 300
GKEESSLMDL ADVFTTPAPP QASDPWGGPA SVPTAVPVAA AASDPWGAPA
310 320 330 340 350
VPPAADPWGG AAPTPASGDP WRPAAPTGPS VDPWGGTPAP AAGEGPTSDP
360 370 380 390 400
WGSADGGAPV SGPPSSDPWA PAPAFSDPWG GSPAKPSSNG TAVGGFDTEP
410 420 430 440 450
DEFSDFDRLR TALPTSGSST GELELLAGEV PARSPGAFDM SGVGGSLAES
460 470 480 490 500
VGSPPPAATP TPTPPTRKTP ESFLGPNAAL VDLDSLVSRP GPTPPGAKAS
510 520 530 540 550
NPFLPSGAPA TGPSVTNPFQ PAPPATLTLN QLRLSPVPPV PGAPPTYISP
560 570
LGGGPGLPPM MPPGPPAPNT NPFLL
Length:575
Mass (Da):60,158
Last modified:November 1, 1998 - v1
Checksum:iD0B770F3B7AB5DDA
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A140TAC3A0A140TAC3_RAT
Epsin-1
Epn1
576Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF018261 mRNA Translation: AAC33823.1
RefSeqiNP_476477.1, NM_057136.1
UniGeneiRn.30007

Genome annotation databases

GeneIDi117277
KEGGirno:117277
UCSCiRGD:619772 rat

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

The bubble's bend - Issue 42 of January 2004

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF018261 mRNA Translation: AAC33823.1
RefSeqiNP_476477.1, NM_057136.1
UniGeneiRn.30007

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EDUX-ray1.80A12-160[»]
1EYHX-ray1.56A15-158[»]
1H0AX-ray1.70A1-158[»]
1KY6X-ray2.00P375-381[»]
DisProtiDP00251
ProteinModelPortaliO88339
SMRiO88339
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250724, 15 interactors
CORUMiO88339
DIPiDIP-40738N
ELMiO88339
IntActiO88339, 4 interactors
MINTiO88339
STRINGi10116.ENSRNOP00000021286

PTM databases

iPTMnetiO88339
PhosphoSitePlusiO88339

Proteomic databases

PaxDbiO88339
PeptideAtlasiO88339
PRIDEiO88339

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117277
KEGGirno:117277
UCSCiRGD:619772 rat

Organism-specific databases

CTDi29924
RGDi619772 Epn1

Phylogenomic databases

eggNOGiKOG2056 Eukaryota
ENOG410XSM0 LUCA
HOGENOMiHOG000008298
HOVERGENiHBG006690
InParanoidiO88339
KOiK12471
OrthoDBiEOG091G0L45
PhylomeDBiO88339

Miscellaneous databases

EvolutionaryTraceiO88339
PROiPR:O88339

Gene expression databases

GenevisibleiO88339 RN

Family and domain databases

CDDicd03571 ENTH_epsin, 1 hit
Gene3Di1.25.40.90, 1 hit
InterProiView protein in InterPro
IPR013809 ENTH
IPR008942 ENTH_VHS
IPR039416 Epsin_ENTH
IPR003903 UIM_dom
PfamiView protein in Pfam
PF01417 ENTH, 1 hit
PF02809 UIM, 2 hits
SMARTiView protein in SMART
SM00273 ENTH, 1 hit
SM00726 UIM, 3 hits
SUPFAMiSSF48464 SSF48464, 1 hit
PROSITEiView protein in PROSITE
PS50942 ENTH, 1 hit
PS50330 UIM, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiEPN1_RAT
AccessioniPrimary (citable) accession number: O88339
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: November 1, 1998
Last modified: September 12, 2018
This is version 156 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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