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Protein

Nidogen-2

Gene

Nid2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.

GO - Molecular functioni

GO - Biological processi

  • cell-matrix adhesion Source: MGI

Keywordsi

Biological processCell adhesion
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-3000157 Laminin interactions

Names & Taxonomyi

Protein namesi
Recommended name:
Nidogen-2
Short name:
NID-2
Alternative name(s):
Entactin-2
Gene namesi
Name:Nid2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1298229 Nid2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Basement membrane, Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30By similarityAdd BLAST30
ChainiPRO_000000767231 – 1403Nidogen-2Add BLAST1373

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi511 ↔ 524By similarity
Disulfide bondi518 ↔ 533By similarity
Disulfide bondi535 ↔ 546By similarity
Glycosylationi681N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi716N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi726N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi786 ↔ 799By similarity
Disulfide bondi793 ↔ 809By similarity
Disulfide bondi811 ↔ 822By similarity
Disulfide bondi828 ↔ 841By similarity
Disulfide bondi835 ↔ 850By similarity
Disulfide bondi852 ↔ 865By similarity
Disulfide bondi875 ↔ 890By similarity
Disulfide bondi882 ↔ 900By similarity
Disulfide bondi902 ↔ 913By similarity
Disulfide bondi919 ↔ 930By similarity
Disulfide bondi924 ↔ 939By similarity
Disulfide bondi941 ↔ 952By similarity
Disulfide bondi968 ↔ 991By similarity
Disulfide bondi1002 ↔ 1009By similarity
Disulfide bondi1011 ↔ 1033By similarity
Disulfide bondi1047 ↔ 1071By similarity
Disulfide bondi1082 ↔ 1089By similarity
Disulfide bondi1091 ↔ 1112By similarity
Glycosylationi1152N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1336Omega-N-methylarginineCombined sources1

Post-translational modificationi

Highly N- and O-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Methylation

Proteomic databases

MaxQBiO88322
PaxDbiO88322
PeptideAtlasiO88322
PRIDEiO88322

PTM databases

iPTMnetiO88322
PhosphoSitePlusiO88322

Expressioni

Gene expression databases

BgeeiENSMUSG00000021806 Expressed in 265 organ(s), highest expression level in humerus cartilage element
CleanExiMM_NID2
ExpressionAtlasiO88322 baseline and differential
GenevisibleiO88322 MM

Interactioni

Subunit structurei

Interacts with LAMA2. Interacts with COL13A1 (By similarity).By similarity

Protein-protein interaction databases

BioGridi201771, 5 interactors
STRINGi10090.ENSMUSP00000022340

Structurei

3D structure databases

ProteinModelPortaliO88322
SMRiO88322
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 274NIDOPROSITE-ProRule annotationAdd BLAST167
Domaini507 – 547EGF-like 1PROSITE-ProRule annotationAdd BLAST41
Domaini551 – 781Nidogen G2 beta-barrelPROSITE-ProRule annotationAdd BLAST231
Domaini782 – 823EGF-like 2PROSITE-ProRule annotationAdd BLAST42
Domaini824 – 862EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini871 – 914EGF-like 4PROSITE-ProRule annotationAdd BLAST44
Domaini915 – 953EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST39
Domaini965 – 1033Thyroglobulin type-1 1PROSITE-ProRule annotationAdd BLAST69
Domaini1044 – 1112Thyroglobulin type-1 2PROSITE-ProRule annotationAdd BLAST69
Repeati1182 – 1225LDL-receptor class B 1Add BLAST44
Repeati1226 – 1268LDL-receptor class B 2Add BLAST43
Repeati1269 – 1313LDL-receptor class B 3Add BLAST45
Repeati1314 – 1355LDL-receptor class B 4Add BLAST42
Repeati1357 – 1401LDL-receptor class B 5Add BLAST45

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi946 – 948Cell attachment site3

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IMYX Eukaryota
ENOG4111DVZ LUCA
GeneTreeiENSGT00910000144086
HOGENOMiHOG000072712
HOVERGENiHBG006498
InParanoidiO88322
KOiK06826
OMAiRHAFCTD
OrthoDBiEOG091G030P
TreeFamiTF320666

Family and domain databases

CDDicd00255 nidG2, 1 hit
cd00191 TY, 2 hits
Gene3Di2.120.10.30, 1 hit
4.10.800.10, 2 hits
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR006605 G2_nidogen/fibulin_G2F
IPR009017 GFP
IPR009030 Growth_fac_rcpt_cys_sf
IPR000033 LDLR_classB_rpt
IPR003886 NIDO_dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF07645 EGF_CA, 2 hits
PF07474 G2F, 1 hit
PF00058 Ldl_recept_b, 2 hits
PF06119 NIDO, 1 hit
PF00086 Thyroglobulin_1, 2 hits
SMARTiView protein in SMART
SM00181 EGF, 5 hits
SM00179 EGF_CA, 5 hits
SM00682 G2F, 1 hit
SM00135 LY, 4 hits
SM00539 NIDO, 1 hit
SM00211 TY, 2 hits
SUPFAMiSSF54511 SSF54511, 1 hit
SSF57184 SSF57184, 1 hit
SSF57610 SSF57610, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS01186 EGF_2, 4 hits
PS50026 EGF_3, 5 hits
PS01187 EGF_CA, 2 hits
PS51120 LDLRB, 4 hits
PS51220 NIDO, 1 hit
PS50993 NIDOGEN_G2, 1 hit
PS00484 THYROGLOBULIN_1_1, 2 hits
PS51162 THYROGLOBULIN_1_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O88322-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFRDPTAGWL TPPSPLSLLV MLLLLSRVGA LRPDELFPYG ESWGDQLLPE
60 70 80 90 100
GDDESSAAVK LAIPLRFYDA QFSSLYVGTN GIISTQDFPR ETQYVDDDFP
110 120 130 140 150
TDFPAIAPFL ADIDTSHSRG RILYREDTSG AVLSLAARYV RTGFPLSGSS
160 170 180 190 200
FTPTHAFLAT WEHVGAYEEV SRGAAPSGEL NTFQAVLASD ESDTYALFLY
210 220 230 240 250
PANGLQFFGT RPKESYNVQL QLPARVGFCR GEADDLKREA LYFSLTNTEQ
260 270 280 290 300
SVKNLYQLSN LGIPGVWAFH IGSRFALDNV RPATVGGDPS TARSSALEHP
310 320 330 340 350
FSHAAALESY TEDSFHYYDE NEEDVEYPPV EPGEAPEGHS RIDVSFNSKA
360 370 380 390 400
DPGLVDVGTS SPGSDRASPW PYPAPGNWPS YRETESASLD PQTKQGRPVG
410 420 430 440 450
EGEVLDFRDP AELLDQMGTR APAPPEADAA LLTPVNEDLG GRNTQSYPEA
460 470 480 490 500
GPVPSEPDVP VPPLEGEVLP HYPESGHVPP LRGGKYVIGL EDHVGSNDQV
510 520 530 540 550
FTYNGANLET CEHSHGRCSQ HAFCTDYTTG FCCHCQSRFY GNGKHCLPEG
560 570 580 590 600
APHRVNGKVS GRLRVGHIPV HFTDVDLHAY IVGNDGRAYT AISHVPQPAA
610 620 630 640 650
QALLPVLPIG GLFGWLFALE KPGSENGFSL TGATFVHDVE VTFHPGEERV
660 670 680 690 700
RITQTAEGLD PENYLSIKTN IEGQVPFIPA NFTAHITPYK EFYHYRDSVV
710 720 730 740 750
TSSSSRSFSL TSGSINQTWS YHIDQNITYQ ACRHAPRHLA IPATQQLTVD
760 770 780 790 800
RAFALYSEDE GVLRFAVTNQ IGPVEVDSAP VGVNPCYDGS HTCDTTARCH
810 820 830 840 850
PGTGVDYTCE CTPGFQGDGR SCVDVNECAT GFHRCGPNSV CVNLVGSYRC
860 870 880 890 900
ECRSGYEFAD DQHTCILIAP PPNPCLDGSH TCAPEGQARC IHHGGSSFSC
910 920 930 940 950
ACLPGFIGTG HQCSDVDECA ENRCHEAAIC YNTPGSFSCR CQPGYRGDGF
960 970 980 990 1000
HCTSDTVPED SISGLKPCEY QQRYAQTQHA YPGSRIHIPQ CDDQGNFVPL
1010 1020 1030 1040 1050
QCHGSTGFCW CVDRNGHEVP GTQTPPGSTP PHCGPPPEPT QRPRTVCERW
1060 1070 1080 1090 1100
RESLLEHYGG TPRDDQYVPQ CDDLGHFIPL QCHGKSDFCW CVDKDGRELQ
1110 1120 1130 1140 1150
GTRSQPGTRP ACIPTVAPPV VRPTPRPDVT PPSVGTFLLY AQGQQIGHLP
1160 1170 1180 1190 1200
LNGSRLQKDA ARTLLSLHGS IVVGIDYDCR ERMVYWTDVA GRTISRASLE
1210 1220 1230 1240 1250
AGAEPETIIT SGLISPEGLA IDHFRRTMYW TDSGLDKIER AELDGSERKV
1260 1270 1280 1290 1300
LFHTDLVNPR AITVDPIRGN LYWTDWNREA PKIETSSLDG ENRRILINKD
1310 1320 1330 1340 1350
IGLPNGLTFD PFSKLLCWAD AGTKKLECTL PDGTGRRVIQ NHLNYPFSIV
1360 1370 1380 1390 1400
SYADHFYHTD WRRDGVISVN KDSGQFTDEF LPEQRSHLYG ITAVYPYCPT

GRK
Length:1,403
Mass (Da):153,913
Last modified:July 27, 2011 - v2
Checksum:iABA0B1DB2C84A27A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A286YCQ5A0A286YCQ5_MOUSE
Nidogen-2
Nid2
1,171Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti197L → F in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti251S → F in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti367A → V in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti375P → H in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti386S → W in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti496S → F in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti651R → C in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti876L → F in BAA32609 (PubMed:9633511).Curated1
Sequence conflicti1151L → F in BAA32609 (PubMed:9633511).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017202 mRNA Translation: BAA32609.1
CH466613 Genomic DNA Translation: EDL01537.1
BC054746 mRNA Translation: AAH54746.1
BC057016 mRNA Translation: AAH57016.1
CCDSiCCDS36813.1
RefSeqiNP_032721.2, NM_008695.2
UniGeneiMm.20348

Genome annotation databases

EnsembliENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806
GeneIDi18074
KEGGimmu:18074
UCSCiuc007siu.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017202 mRNA Translation: BAA32609.1
CH466613 Genomic DNA Translation: EDL01537.1
BC054746 mRNA Translation: AAH54746.1
BC057016 mRNA Translation: AAH57016.1
CCDSiCCDS36813.1
RefSeqiNP_032721.2, NM_008695.2
UniGeneiMm.20348

3D structure databases

ProteinModelPortaliO88322
SMRiO88322
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201771, 5 interactors
STRINGi10090.ENSMUSP00000022340

PTM databases

iPTMnetiO88322
PhosphoSitePlusiO88322

Proteomic databases

MaxQBiO88322
PaxDbiO88322
PeptideAtlasiO88322
PRIDEiO88322

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806
GeneIDi18074
KEGGimmu:18074
UCSCiuc007siu.1 mouse

Organism-specific databases

CTDi22795
MGIiMGI:1298229 Nid2

Phylogenomic databases

eggNOGiENOG410IMYX Eukaryota
ENOG4111DVZ LUCA
GeneTreeiENSGT00910000144086
HOGENOMiHOG000072712
HOVERGENiHBG006498
InParanoidiO88322
KOiK06826
OMAiRHAFCTD
OrthoDBiEOG091G030P
TreeFamiTF320666

Enzyme and pathway databases

ReactomeiR-MMU-3000157 Laminin interactions

Miscellaneous databases

PROiPR:O88322
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021806 Expressed in 265 organ(s), highest expression level in humerus cartilage element
CleanExiMM_NID2
ExpressionAtlasiO88322 baseline and differential
GenevisibleiO88322 MM

Family and domain databases

CDDicd00255 nidG2, 1 hit
cd00191 TY, 2 hits
Gene3Di2.120.10.30, 1 hit
4.10.800.10, 2 hits
InterProiView protein in InterPro
IPR011042 6-blade_b-propeller_TolB-like
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR006605 G2_nidogen/fibulin_G2F
IPR009017 GFP
IPR009030 Growth_fac_rcpt_cys_sf
IPR000033 LDLR_classB_rpt
IPR003886 NIDO_dom
IPR000716 Thyroglobulin_1
IPR036857 Thyroglobulin_1_sf
PfamiView protein in Pfam
PF07645 EGF_CA, 2 hits
PF07474 G2F, 1 hit
PF00058 Ldl_recept_b, 2 hits
PF06119 NIDO, 1 hit
PF00086 Thyroglobulin_1, 2 hits
SMARTiView protein in SMART
SM00181 EGF, 5 hits
SM00179 EGF_CA, 5 hits
SM00682 G2F, 1 hit
SM00135 LY, 4 hits
SM00539 NIDO, 1 hit
SM00211 TY, 2 hits
SUPFAMiSSF54511 SSF54511, 1 hit
SSF57184 SSF57184, 1 hit
SSF57610 SSF57610, 2 hits
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 3 hits
PS01186 EGF_2, 4 hits
PS50026 EGF_3, 5 hits
PS01187 EGF_CA, 2 hits
PS51120 LDLRB, 4 hits
PS51220 NIDO, 1 hit
PS50993 NIDOGEN_G2, 1 hit
PS00484 THYROGLOBULIN_1_1, 2 hits
PS51162 THYROGLOBULIN_1_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiNID2_MOUSE
AccessioniPrimary (citable) accession number: O88322
Secondary accession number(s): Q7TQF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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