UniProtKB - O88322 (NID2_MOUSE)
Protein
Nidogen-2
Gene
Nid2
Organism
Mus musculus (Mouse)
Status
Functioni
Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.
GO - Molecular functioni
- calcium ion binding Source: InterPro
GO - Biological processi
- cell-matrix adhesion Source: MGI
Keywordsi
Biological process | Cell adhesion |
Ligand | Calcium |
Enzyme and pathway databases
Reactomei | R-MMU-3000157, Laminin interactions |
Names & Taxonomyi
Protein namesi | Recommended name: Nidogen-2Short name: NID-2 Alternative name(s): Entactin-2 |
Gene namesi | Name:Nid2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1298229, Nid2 |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: GO_Central
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- basement membrane Source: MGI
- cell surface Source: MGI
- collagen-containing extracellular matrix Source: UniProtKB
- extracellular matrix Source: MGI
Keywords - Cellular componenti
Basement membrane, Extracellular matrix, SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 30 | By similarityAdd BLAST | 30 | |
ChainiPRO_0000007672 | 31 – 1403 | Nidogen-2Add BLAST | 1373 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 511 ↔ 524 | By similarity | ||
Disulfide bondi | 518 ↔ 533 | By similarity | ||
Disulfide bondi | 535 ↔ 546 | By similarity | ||
Glycosylationi | 681 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Glycosylationi | 716 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 726 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 786 ↔ 799 | By similarity | ||
Disulfide bondi | 793 ↔ 809 | By similarity | ||
Disulfide bondi | 811 ↔ 822 | By similarity | ||
Disulfide bondi | 828 ↔ 841 | By similarity | ||
Disulfide bondi | 835 ↔ 850 | By similarity | ||
Disulfide bondi | 852 ↔ 865 | By similarity | ||
Disulfide bondi | 875 ↔ 890 | By similarity | ||
Disulfide bondi | 882 ↔ 900 | By similarity | ||
Disulfide bondi | 902 ↔ 913 | By similarity | ||
Disulfide bondi | 919 ↔ 930 | By similarity | ||
Disulfide bondi | 924 ↔ 939 | By similarity | ||
Disulfide bondi | 941 ↔ 952 | By similarity | ||
Disulfide bondi | 968 ↔ 991 | By similarity | ||
Disulfide bondi | 1002 ↔ 1009 | By similarity | ||
Disulfide bondi | 1011 ↔ 1033 | By similarity | ||
Disulfide bondi | 1047 ↔ 1071 | By similarity | ||
Disulfide bondi | 1082 ↔ 1089 | By similarity | ||
Disulfide bondi | 1091 ↔ 1112 | By similarity | ||
Glycosylationi | 1152 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 1336 | Omega-N-methylarginineCombined sources | 1 |
Post-translational modificationi
Highly N- and O-glycosylated.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, MethylationProteomic databases
jPOSTi | O88322 |
MaxQBi | O88322 |
PaxDbi | O88322 |
PeptideAtlasi | O88322 |
PRIDEi | O88322 |
ProteomicsDBi | 252965 |
PTM databases
GlyGeni | O88322, 4 sites |
iPTMneti | O88322 |
PhosphoSitePlusi | O88322 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000021806, Expressed in humerus cartilage element and 281 other tissues |
ExpressionAtlasi | O88322, baseline and differential |
Genevisiblei | O88322, MM |
Interactioni
Subunit structurei
Interacts with LAMA2.
Interacts with COL13A1 (By similarity).
Interacts with EFEMP2 (PubMed:17324935).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 201771, 9 interactors |
STRINGi | 10090.ENSMUSP00000022340 |
Miscellaneous databases
RNActi | O88322, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 274 | NIDOPROSITE-ProRule annotationAdd BLAST | 167 | |
Domaini | 507 – 547 | EGF-like 1PROSITE-ProRule annotationAdd BLAST | 41 | |
Domaini | 551 – 781 | Nidogen G2 beta-barrelPROSITE-ProRule annotationAdd BLAST | 231 | |
Domaini | 782 – 823 | EGF-like 2PROSITE-ProRule annotationAdd BLAST | 42 | |
Domaini | 824 – 862 | EGF-like 3; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 871 – 914 | EGF-like 4PROSITE-ProRule annotationAdd BLAST | 44 | |
Domaini | 915 – 953 | EGF-like 5; calcium-bindingPROSITE-ProRule annotationAdd BLAST | 39 | |
Domaini | 965 – 1033 | Thyroglobulin type-1 1PROSITE-ProRule annotationAdd BLAST | 69 | |
Domaini | 1044 – 1112 | Thyroglobulin type-1 2PROSITE-ProRule annotationAdd BLAST | 69 | |
Repeati | 1182 – 1225 | LDL-receptor class B 1Add BLAST | 44 | |
Repeati | 1226 – 1268 | LDL-receptor class B 2Add BLAST | 43 | |
Repeati | 1269 – 1313 | LDL-receptor class B 3Add BLAST | 45 | |
Repeati | 1314 – 1355 | LDL-receptor class B 4Add BLAST | 42 | |
Repeati | 1357 – 1401 | LDL-receptor class B 5Add BLAST | 45 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 946 – 948 | Cell attachment site | 3 |
Keywords - Domaini
EGF-like domain, Repeat, SignalPhylogenomic databases
eggNOGi | KOG1214, Eukaryota |
GeneTreei | ENSGT00940000157901 |
HOGENOMi | CLU_003163_1_0_1 |
InParanoidi | O88322 |
OMAi | CERWKES |
OrthoDBi | 95286at2759 |
TreeFami | TF320666 |
Family and domain databases
CDDi | cd00255, nidG2, 1 hit cd00191, TY, 2 hits |
Gene3Di | 2.120.10.30, 1 hit 4.10.800.10, 2 hits |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR006605, G2_nidogen/fibulin_G2F IPR009017, GFP IPR009030, Growth_fac_rcpt_cys_sf IPR000033, LDLR_classB_rpt IPR003886, NIDO_dom IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
Pfami | View protein in Pfam PF07645, EGF_CA, 2 hits PF07474, G2F, 1 hit PF00058, Ldl_recept_b, 2 hits PF06119, NIDO, 1 hit PF00086, Thyroglobulin_1, 2 hits |
SMARTi | View protein in SMART SM00181, EGF, 5 hits SM00179, EGF_CA, 5 hits SM00682, G2F, 1 hit SM00135, LY, 4 hits SM00539, NIDO, 1 hit SM00211, TY, 2 hits |
SUPFAMi | SSF54511, SSF54511, 1 hit SSF57184, SSF57184, 1 hit SSF57610, SSF57610, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 3 hits PS01186, EGF_2, 4 hits PS50026, EGF_3, 5 hits PS01187, EGF_CA, 2 hits PS51120, LDLRB, 4 hits PS51220, NIDO, 1 hit PS50993, NIDOGEN_G2, 1 hit PS00484, THYROGLOBULIN_1_1, 2 hits PS51162, THYROGLOBULIN_1_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O88322-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRDPTAGWL TPPSPLSLLV MLLLLSRVGA LRPDELFPYG ESWGDQLLPE
60 70 80 90 100
GDDESSAAVK LAIPLRFYDA QFSSLYVGTN GIISTQDFPR ETQYVDDDFP
110 120 130 140 150
TDFPAIAPFL ADIDTSHSRG RILYREDTSG AVLSLAARYV RTGFPLSGSS
160 170 180 190 200
FTPTHAFLAT WEHVGAYEEV SRGAAPSGEL NTFQAVLASD ESDTYALFLY
210 220 230 240 250
PANGLQFFGT RPKESYNVQL QLPARVGFCR GEADDLKREA LYFSLTNTEQ
260 270 280 290 300
SVKNLYQLSN LGIPGVWAFH IGSRFALDNV RPATVGGDPS TARSSALEHP
310 320 330 340 350
FSHAAALESY TEDSFHYYDE NEEDVEYPPV EPGEAPEGHS RIDVSFNSKA
360 370 380 390 400
DPGLVDVGTS SPGSDRASPW PYPAPGNWPS YRETESASLD PQTKQGRPVG
410 420 430 440 450
EGEVLDFRDP AELLDQMGTR APAPPEADAA LLTPVNEDLG GRNTQSYPEA
460 470 480 490 500
GPVPSEPDVP VPPLEGEVLP HYPESGHVPP LRGGKYVIGL EDHVGSNDQV
510 520 530 540 550
FTYNGANLET CEHSHGRCSQ HAFCTDYTTG FCCHCQSRFY GNGKHCLPEG
560 570 580 590 600
APHRVNGKVS GRLRVGHIPV HFTDVDLHAY IVGNDGRAYT AISHVPQPAA
610 620 630 640 650
QALLPVLPIG GLFGWLFALE KPGSENGFSL TGATFVHDVE VTFHPGEERV
660 670 680 690 700
RITQTAEGLD PENYLSIKTN IEGQVPFIPA NFTAHITPYK EFYHYRDSVV
710 720 730 740 750
TSSSSRSFSL TSGSINQTWS YHIDQNITYQ ACRHAPRHLA IPATQQLTVD
760 770 780 790 800
RAFALYSEDE GVLRFAVTNQ IGPVEVDSAP VGVNPCYDGS HTCDTTARCH
810 820 830 840 850
PGTGVDYTCE CTPGFQGDGR SCVDVNECAT GFHRCGPNSV CVNLVGSYRC
860 870 880 890 900
ECRSGYEFAD DQHTCILIAP PPNPCLDGSH TCAPEGQARC IHHGGSSFSC
910 920 930 940 950
ACLPGFIGTG HQCSDVDECA ENRCHEAAIC YNTPGSFSCR CQPGYRGDGF
960 970 980 990 1000
HCTSDTVPED SISGLKPCEY QQRYAQTQHA YPGSRIHIPQ CDDQGNFVPL
1010 1020 1030 1040 1050
QCHGSTGFCW CVDRNGHEVP GTQTPPGSTP PHCGPPPEPT QRPRTVCERW
1060 1070 1080 1090 1100
RESLLEHYGG TPRDDQYVPQ CDDLGHFIPL QCHGKSDFCW CVDKDGRELQ
1110 1120 1130 1140 1150
GTRSQPGTRP ACIPTVAPPV VRPTPRPDVT PPSVGTFLLY AQGQQIGHLP
1160 1170 1180 1190 1200
LNGSRLQKDA ARTLLSLHGS IVVGIDYDCR ERMVYWTDVA GRTISRASLE
1210 1220 1230 1240 1250
AGAEPETIIT SGLISPEGLA IDHFRRTMYW TDSGLDKIER AELDGSERKV
1260 1270 1280 1290 1300
LFHTDLVNPR AITVDPIRGN LYWTDWNREA PKIETSSLDG ENRRILINKD
1310 1320 1330 1340 1350
IGLPNGLTFD PFSKLLCWAD AGTKKLECTL PDGTGRRVIQ NHLNYPFSIV
1360 1370 1380 1390 1400
SYADHFYHTD WRRDGVISVN KDSGQFTDEF LPEQRSHLYG ITAVYPYCPT
GRK
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A286YCQ5 | A0A286YCQ5_MOUSE | Nidogen-2 | Nid2 | 1,171 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 197 | L → F in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 251 | S → F in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 367 | A → V in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 375 | P → H in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 386 | S → W in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 496 | S → F in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 651 | R → C in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 876 | L → F in BAA32609 (PubMed:9633511).Curated | 1 | |
Sequence conflicti | 1151 | L → F in BAA32609 (PubMed:9633511).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017202 mRNA Translation: BAA32609.1 CH466613 Genomic DNA Translation: EDL01537.1 BC054746 mRNA Translation: AAH54746.1 BC057016 mRNA Translation: AAH57016.1 |
CCDSi | CCDS36813.1 |
RefSeqi | NP_032721.2, NM_008695.2 |
Genome annotation databases
Ensembli | ENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806 |
GeneIDi | 18074 |
KEGGi | mmu:18074 |
UCSCi | uc007siu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB017202 mRNA Translation: BAA32609.1 CH466613 Genomic DNA Translation: EDL01537.1 BC054746 mRNA Translation: AAH54746.1 BC057016 mRNA Translation: AAH57016.1 |
CCDSi | CCDS36813.1 |
RefSeqi | NP_032721.2, NM_008695.2 |
3D structure databases
SMRi | O88322 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201771, 9 interactors |
STRINGi | 10090.ENSMUSP00000022340 |
PTM databases
GlyGeni | O88322, 4 sites |
iPTMneti | O88322 |
PhosphoSitePlusi | O88322 |
Proteomic databases
jPOSTi | O88322 |
MaxQBi | O88322 |
PaxDbi | O88322 |
PeptideAtlasi | O88322 |
PRIDEi | O88322 |
ProteomicsDBi | 252965 |
Protocols and materials databases
Antibodypediai | 10737, 169 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022340; ENSMUSP00000022340; ENSMUSG00000021806 |
GeneIDi | 18074 |
KEGGi | mmu:18074 |
UCSCi | uc007siu.1, mouse |
Organism-specific databases
CTDi | 22795 |
MGIi | MGI:1298229, Nid2 |
Phylogenomic databases
eggNOGi | KOG1214, Eukaryota |
GeneTreei | ENSGT00940000157901 |
HOGENOMi | CLU_003163_1_0_1 |
InParanoidi | O88322 |
OMAi | CERWKES |
OrthoDBi | 95286at2759 |
TreeFami | TF320666 |
Enzyme and pathway databases
Reactomei | R-MMU-3000157, Laminin interactions |
Miscellaneous databases
BioGRID-ORCSi | 18074, 0 hits in 52 CRISPR screens |
ChiTaRSi | Nid2, mouse |
PROi | PR:O88322 |
RNActi | O88322, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000021806, Expressed in humerus cartilage element and 281 other tissues |
ExpressionAtlasi | O88322, baseline and differential |
Genevisiblei | O88322, MM |
Family and domain databases
CDDi | cd00255, nidG2, 1 hit cd00191, TY, 2 hits |
Gene3Di | 2.120.10.30, 1 hit 4.10.800.10, 2 hits |
InterProi | View protein in InterPro IPR011042, 6-blade_b-propeller_TolB-like IPR001881, EGF-like_Ca-bd_dom IPR000742, EGF-like_dom IPR000152, EGF-type_Asp/Asn_hydroxyl_site IPR018097, EGF_Ca-bd_CS IPR006605, G2_nidogen/fibulin_G2F IPR009017, GFP IPR009030, Growth_fac_rcpt_cys_sf IPR000033, LDLR_classB_rpt IPR003886, NIDO_dom IPR000716, Thyroglobulin_1 IPR036857, Thyroglobulin_1_sf |
Pfami | View protein in Pfam PF07645, EGF_CA, 2 hits PF07474, G2F, 1 hit PF00058, Ldl_recept_b, 2 hits PF06119, NIDO, 1 hit PF00086, Thyroglobulin_1, 2 hits |
SMARTi | View protein in SMART SM00181, EGF, 5 hits SM00179, EGF_CA, 5 hits SM00682, G2F, 1 hit SM00135, LY, 4 hits SM00539, NIDO, 1 hit SM00211, TY, 2 hits |
SUPFAMi | SSF54511, SSF54511, 1 hit SSF57184, SSF57184, 1 hit SSF57610, SSF57610, 2 hits |
PROSITEi | View protein in PROSITE PS00010, ASX_HYDROXYL, 3 hits PS01186, EGF_2, 4 hits PS50026, EGF_3, 5 hits PS01187, EGF_CA, 2 hits PS51120, LDLRB, 4 hits PS51220, NIDO, 1 hit PS50993, NIDOGEN_G2, 1 hit PS00484, THYROGLOBULIN_1_1, 2 hits PS51162, THYROGLOBULIN_1_2, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NID2_MOUSE | |
Accessioni | O88322Primary (citable) accession number: O88322 Secondary accession number(s): Q7TQF0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | July 27, 2011 | |
Last modified: | April 7, 2021 | |
This is version 186 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot