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Protein

Collagen alpha-1(V) chain

Gene

Col5a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin (By similarity). Transcriptionally activated by CEBPZ, which recognizes a CCAAT-like motif, CAAAT in the COL5A1 promoter.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding

Enzyme and pathway databases

ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1474244 Extracellular matrix organization
R-MMU-1650814 Collagen biosynthesis and modifying enzymes
R-MMU-186797 Signaling by PDGF
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-216083 Integrin cell surface interactions
R-MMU-3000171 Non-integrin membrane-ECM interactions
R-MMU-3000178 ECM proteoglycans
R-MMU-419037 NCAM1 interactions
R-MMU-8874081 MET activates PTK2 signaling
R-MMU-8948216 Collagen chain trimerization

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-1(V) chain
Gene namesi
Name:Col5a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:88457 Col5a1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 36Sequence analysisAdd BLAST36
ChainiPRO_000004176137 – 1838Collagen alpha-1(V) chainAdd BLAST1802

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei234SulfotyrosineSequence analysis1
Modified residuei236SulfotyrosineSequence analysis1
Modified residuei240SulfotyrosineSequence analysis1
Modified residuei262SulfotyrosineSequence analysis1
Modified residuei263SulfotyrosineSequence analysis1
Modified residuei5704-hydroxyprolineBy similarity1
Modified residuei5764-hydroxyprolineBy similarity1
Modified residuei6214-hydroxyprolineBy similarity1
Modified residuei6275-hydroxylysineBy similarity1
Modified residuei6394-hydroxyprolineBy similarity1
Modified residuei6425-hydroxylysineBy similarity1
Modified residuei6484-hydroxyprolineBy similarity1
Modified residuei6544-hydroxyprolineBy similarity1
Modified residuei6574-hydroxyprolineBy similarity1
Modified residuei6754-hydroxyprolineBy similarity1
Modified residuei6784-hydroxyprolineBy similarity1
Modified residuei6803-hydroxyprolineBy similarity1
Modified residuei6863-hydroxyprolineBy similarity1
Modified residuei6904-hydroxyprolineBy similarity1
Modified residuei6964-hydroxyprolineBy similarity1
Modified residuei7054-hydroxyprolineBy similarity1
Modified residuei7085-hydroxylysineBy similarity1
Modified residuei7174-hydroxyprolineBy similarity1
Modified residuei7204-hydroxyprolineBy similarity1
Modified residuei7264-hydroxyprolineBy similarity1
Modified residuei7324-hydroxyprolineBy similarity1
Modified residuei7445-hydroxylysineBy similarity1
Modified residuei7504-hydroxyprolineBy similarity1
Modified residuei7564-hydroxyprolineBy similarity1
Modified residuei7624-hydroxyprolineBy similarity1
Modified residuei7654-hydroxyprolineBy similarity1
Modified residuei7714-hydroxyprolineBy similarity1
Modified residuei7745-hydroxylysineBy similarity1
Modified residuei7804-hydroxyprolineBy similarity1
Modified residuei7894-hydroxyprolineBy similarity1
Modified residuei7955-hydroxylysineBy similarity1
Modified residuei8045-hydroxylysineBy similarity1
Modified residuei8075-hydroxylysineBy similarity1
Modified residuei8105-hydroxylysineBy similarity1
Modified residuei8164-hydroxyprolineBy similarity1
Modified residuei8195-hydroxylysineBy similarity1
Modified residuei8344-hydroxyprolineBy similarity1
Modified residuei8465-hydroxylysineBy similarity1
Modified residuei8645-hydroxylysineBy similarity1
Modified residuei8704-hydroxyprolineBy similarity1
Modified residuei8734-hydroxyprolineBy similarity1
Modified residuei8764-hydroxyprolineBy similarity1
Modified residuei8825-hydroxylysineBy similarity1
Modified residuei8884-hydroxyprolineBy similarity1
Modified residuei8914-hydroxyprolineBy similarity1
Modified residuei8975-hydroxylysineBy similarity1
Modified residuei9034-hydroxyprolineBy similarity1
Modified residuei9064-hydroxyprolineBy similarity1
Modified residuei9304-hydroxyprolineBy similarity1
Modified residuei9454-hydroxyprolineBy similarity1
Modified residuei10174-hydroxyprolineBy similarity1
Modified residuei10204-hydroxyprolineBy similarity1
Modified residuei10234-hydroxyprolineBy similarity1
Modified residuei10294-hydroxyprolineBy similarity1
Modified residuei12214-hydroxyprolineBy similarity1
Modified residuei12244-hydroxyprolineBy similarity1
Modified residuei14674-hydroxyprolineBy similarity1
Modified residuei14704-hydroxyprolineBy similarity1
Modified residuei1601SulfotyrosineSequence analysis1
Modified residuei1604SulfotyrosineSequence analysis1

Post-translational modificationi

Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.By similarity
Sulfated on 40% of tyrosines.By similarity

Keywords - PTMi

Hydroxylation, Sulfation

Proteomic databases

MaxQBiO88207
PaxDbiO88207
PeptideAtlasiO88207
PRIDEiO88207

PTM databases

iPTMnetiO88207
PhosphoSitePlusiO88207

Expressioni

Tissue specificityi

Widely expressed (PubMed:22149965). Isoform 2 is more highly expressed in liver, kidney and lung (PubMed:22149965).1 Publication

Developmental stagei

Detected at E11, E12, E14, E16 and E18. Expressed in limbs, vertebrae, heart, brain, liver, intestine, tongue, tail, skin, calvaria, lung and kidney of E18 embryos (PubMed:9582436). Isoform 2 is detected at E13.5 and its expression increases from E13.5 to 5 weeks after birth more specially in liver, lung, ileum and heart. The expression slightly decreases after 15 weeks (PubMed:22149965).2 Publications

Gene expression databases

BgeeiENSMUSG00000026837 Expressed in 283 organ(s), highest expression level in umbilical cord
CleanExiMM_COL5A1
ExpressionAtlasiO88207 baseline and differential
GenevisibleiO88207 MM

Interactioni

Subunit structurei

Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198821, 5 interactors
ComplexPortaliCPX-2962 Collagen type V trimer variant 1
CPX-2963 Collagen type V trimer variant 2
CPX-2964 Collagen type V trimer variant 3
CPX-2977 Collagen type XI trimer variant 3
IntActiO88207, 1 interactor
STRINGi10090.ENSMUSP00000028280

Structurei

3D structure databases

ProteinModelPortaliO88207
SMRiO88207
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 244Laminin G-likeAdd BLAST173
Domaini1609 – 1837Fibrillar collagen NC1PROSITE-ProRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni231 – 443Nonhelical regionBy similarityAdd BLAST213
Regioni444 – 558Interrupted collagenous regionBy similarityAdd BLAST115
Regioni559 – 1570Triple-helical regionBy similarityAdd BLAST1012
Regioni1571 – 1605Nonhelical regionBy similarityAdd BLAST35

Sequence similaritiesi

Belongs to the fibrillar collagen family.PROSITE-ProRule annotation

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3544 Eukaryota
ENOG410XNMM LUCA
GeneTreeiENSGT00900000140789
HOGENOMiHOG000085654
HOVERGENiHBG004933
InParanoidiO88207
KOiK19721
OMAiNITYNCY
OrthoDBiEOG091G01AE
PhylomeDBiO88207
TreeFamiTF323987

Family and domain databases

InterProiView protein in InterPro
IPR008160 Collagen
IPR013320 ConA-like_dom_sf
IPR000885 Fib_collagen_C
IPR001791 Laminin_G
PfamiView protein in Pfam
PF01410 COLFI, 1 hit
PF01391 Collagen, 4 hits
PF02210 Laminin_G_2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002078 Fib_collagen_C, 1 hit
SMARTiView protein in SMART
SM00038 COLFI, 1 hit
SM00210 TSPN, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS51461 NC1_FIB, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O88207-1) [UniParc]FASTAAdd to basket
Also known as: A1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDVHTRWKAA RPGALLLSSP LLLFLLLLWA PPSSRAAQPA DLLEMLDFHN
60 70 80 90 100
LPSGVTKTTG FCATRRSSSE PDVAYRVSKD AQLSMPTKQL YPESGFPEDF
110 120 130 140 150
SILTTVKAKK GSQAFLVSIY NEQGIQQLGL ELGRSPVFLY EDHTGKPGPE
160 170 180 190 200
EYPLFPGINL SDGKWHRIAL SVYKKNVTLI LDCKKKITKF LSRSDHPIID
210 220 230 240 250
TNGIVMFGSR ILDDEIFEGD IQQLLFVSDN RAAYDYCEHY SPDCDTAVPD
260 270 280 290 300
TPQSQDPNPD EYYPEGEGET YYYEYPYYED PEDPGKEPAP TQKPVEAARE
310 320 330 340 350
TTEVPEEQTQ PLPEAPTVPE TSDTADKEDS LGIGDYDYVP PDDYYTPPPY
360 370 380 390 400
EDFGYGEGVE NPDQPTNPDS GAEVPTSTTV TSNTSNPAPG EGKDDLGGEF
410 420 430 440 450
TEETIKNLEE NYYDPYFDPD SDSSVSPSEI GPGMPANQDT IFEGIGGPRG
460 470 480 490 500
EKGQKGEPAI IEPGMLIEGP PGPEGPAGLP GPPGTTGPTG QMGDPGERGP
510 520 530 540 550
PGRPGLPGAD GLPGPPGTML MLPFRFGGGG DAGSKGPMVS AQESQAQAIL
560 570 580 590 600
QQARLALRGP AGPMGLTGRP GPMGPPGSGG LKGEPGDMGP QGPRGVQGPP
610 620 630 640 650
GPTGKPGRRG RAGSDGARGM PGQTGPKGDR GFDGLAGLPG EKGHRGDPGP
660 670 680 690 700
SGPPGIPGDD GERGDDGEVG PRGLPGEPGP RGLLGPKGPP GPPGPPGVTG
710 720 730 740 750
MDGQPGPKGN VGPQGEPGPP GQQGNPGAQG LPGPQGAIGP PGEKGPLGKP
760 770 780 790 800
GLPGMPGADG PPGHPGKEGP PGEKGGQGPP GPQGPIGYPG PRGVKGADGI
810 820 830 840 850
RGLKGTKGEK GEDGFPGFKG DMGIKGDRGE IGPPGPRGED GPEGPKGRGG
860 870 880 890 900
PNGDPGPLGP TGEKGKLGVP GLPGYPGRQG PKGSIGFPGF PGANGEKGGR
910 920 930 940 950
GTPGKPGPRG QRGPTGPRGE RGPRGITGKP GPKGNSGGDG PAGPPGERGP
960 970 980 990 1000
NGPQGPTGFP GPKGPPGPPG KDGLPGHPGQ RGETGFQGKT GPPGPPGVVG
1010 1020 1030 1040 1050
PQGPTGETGP MGERGHPGPP GPPGEQGLPG AAGKEGTKGD PGPAGLPGKD
1060 1070 1080 1090 1100
GPPGLRGFPG DRGLPGPVGA LGLKGSEGPP GPPGPAGSPG ERGPAGAAGP
1110 1120 1130 1140 1150
IGIPGRPGPQ GPPGPAGEKG LPGEKGPQGP AGRDGLQGPV GLPGPAGPVG
1160 1170 1180 1190 1200
PPGEDGDKGE IGEPGQKGSK GDKGEQGPPG PTGPQGPIGQ PGPSGADGEP
1210 1220 1230 1240 1250
GPRGQQGLFG QKGDEGSRGF PGPPGPVGLQ GLPGPPGEKG ETGDVGQMGP
1260 1270 1280 1290 1300
PGPPGPRGPS GAPGADGPQG PPGGIGNPGA VGEKGEPGEA GDPGLPGEGG
1310 1320 1330 1340 1350
PLGPKGERGE KGEAGPSGAA GPPGPKGPPG DDGPKGSPGP VGFPGDPGPP
1360 1370 1380 1390 1400
GEPGPAGQDG PPGDKGDDGE PGQTGSPGPT GEPGPSGPPG KRGPPGPAGP
1410 1420 1430 1440 1450
EGRQGEKGAK GEAGLEGPPG KTGPIGPQGA PGKPGPDGLR GIPGPVGEQG
1460 1470 1480 1490 1500
LPGSPGPDGP PGPMGPPGLP GLKGDSGPKG EKGHPGLIGL IGPPGEQGEK
1510 1520 1530 1540 1550
GDRGLPGPQG SSGPKGDQGI TGPSGPLGPP GPPGLPGPPG PKGAKGSSGP
1560 1570 1580 1590 1600
TGPKGEAGHP GLPGPPGPPG EVIQPLPIQA SRTRRNIDAS QLLDDGAGES
1610 1620 1630 1640 1650
YVDYADGMEE IFGSLNSLKL EIEQMKRPLG TQQNPARTCK DLQLCHPDFP
1660 1670 1680 1690 1700
DGEYWVDPNQ GCSRDSFKVY CNFTAGGSTC VFPDKKSEGA RITSWPKENP
1710 1720 1730 1740 1750
GSWFSEFKRG KLLSYVDAEG NPVGVVQMTF LRLLSASAHQ NVTYNCYQSV
1760 1770 1780 1790 1800
AWQDAATGSY DKAIRFLGSN DEEMSYDNNP YIRALVDGCA TKKGYQKTVL
1810 1820 1830
EIDTPKVEQV PIVDIMFNDF GEASQKFGFE VGPACFLG
Length:1,838
Mass (Da):183,677
Last modified:February 6, 2007 - v2
Checksum:iD20F4E5198A09ECF
GO
Isoform 2 (identifier: O88207-2) [UniParc]FASTAAdd to basket
Also known as: B1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     1690-1712: ARITSWPKENPGSWFSEFKRGKL → SKMARWPKEQPSTWYSQYKRGSL

Show »
Length:1,838
Mass (Da):183,770
Checksum:iBD7D2D71DFDD86C9
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3KGE7A3KGE7_MOUSE
Collagen alpha-1(V) chain
Col5a1
349Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1097A → S in BAA28786 (PubMed:9582436).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0596561690 – 1712ARITS…KRGKL → SKMARWPKEQPSTWYSQYKR GSL in isoform 2. Add BLAST23

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009993 mRNA Translation: BAA28786.1
AL731778, AL732513 Genomic DNA Translation: CAM46247.1
AB098608 Genomic DNA Translation: BAD26732.1
AK148055 mRNA Translation: BAE28315.1
CCDSiCCDS15831.1 [O88207-1]
RefSeqiNP_056549.2, NM_015734.2 [O88207-1]
XP_006497707.1, XM_006497644.3 [O88207-2]
UniGeneiMm.7281

Genome annotation databases

EnsembliENSMUST00000028280; ENSMUSP00000028280; ENSMUSG00000026837 [O88207-1]
GeneIDi12831
KEGGimmu:12831
UCSCiuc008ixt.2 mouse [O88207-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB009993 mRNA Translation: BAA28786.1
AL731778, AL732513 Genomic DNA Translation: CAM46247.1
AB098608 Genomic DNA Translation: BAD26732.1
AK148055 mRNA Translation: BAE28315.1
CCDSiCCDS15831.1 [O88207-1]
RefSeqiNP_056549.2, NM_015734.2 [O88207-1]
XP_006497707.1, XM_006497644.3 [O88207-2]
UniGeneiMm.7281

3D structure databases

ProteinModelPortaliO88207
SMRiO88207
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198821, 5 interactors
ComplexPortaliCPX-2962 Collagen type V trimer variant 1
CPX-2963 Collagen type V trimer variant 2
CPX-2964 Collagen type V trimer variant 3
CPX-2977 Collagen type XI trimer variant 3
IntActiO88207, 1 interactor
STRINGi10090.ENSMUSP00000028280

PTM databases

iPTMnetiO88207
PhosphoSitePlusiO88207

Proteomic databases

MaxQBiO88207
PaxDbiO88207
PeptideAtlasiO88207
PRIDEiO88207

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028280; ENSMUSP00000028280; ENSMUSG00000026837 [O88207-1]
GeneIDi12831
KEGGimmu:12831
UCSCiuc008ixt.2 mouse [O88207-1]

Organism-specific databases

CTDi1289
MGIiMGI:88457 Col5a1

Phylogenomic databases

eggNOGiKOG3544 Eukaryota
ENOG410XNMM LUCA
GeneTreeiENSGT00900000140789
HOGENOMiHOG000085654
HOVERGENiHBG004933
InParanoidiO88207
KOiK19721
OMAiNITYNCY
OrthoDBiEOG091G01AE
PhylomeDBiO88207
TreeFamiTF323987

Enzyme and pathway databases

ReactomeiR-MMU-1442490 Collagen degradation
R-MMU-1474244 Extracellular matrix organization
R-MMU-1650814 Collagen biosynthesis and modifying enzymes
R-MMU-186797 Signaling by PDGF
R-MMU-2022090 Assembly of collagen fibrils and other multimeric structures
R-MMU-216083 Integrin cell surface interactions
R-MMU-3000171 Non-integrin membrane-ECM interactions
R-MMU-3000178 ECM proteoglycans
R-MMU-419037 NCAM1 interactions
R-MMU-8874081 MET activates PTK2 signaling
R-MMU-8948216 Collagen chain trimerization

Miscellaneous databases

ChiTaRSiCol5a1 mouse
PROiPR:O88207
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026837 Expressed in 283 organ(s), highest expression level in umbilical cord
CleanExiMM_COL5A1
ExpressionAtlasiO88207 baseline and differential
GenevisibleiO88207 MM

Family and domain databases

InterProiView protein in InterPro
IPR008160 Collagen
IPR013320 ConA-like_dom_sf
IPR000885 Fib_collagen_C
IPR001791 Laminin_G
PfamiView protein in Pfam
PF01410 COLFI, 1 hit
PF01391 Collagen, 4 hits
PF02210 Laminin_G_2, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD002078 Fib_collagen_C, 1 hit
SMARTiView protein in SMART
SM00038 COLFI, 1 hit
SM00210 TSPN, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS51461 NC1_FIB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCO5A1_MOUSE
AccessioniPrimary (citable) accession number: O88207
Secondary accession number(s): A3KGE3, Q3UG94, Q6F6B0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: February 6, 2007
Last modified: October 10, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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