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Protein

E3 ubiquitin-protein ligase TTC3

Gene

Ttc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that mediates the ubiquitination and subsequent degradation of phosphorylated Akt (AKT1, AKT2 and AKT3) in the nucleus. Acts as a terminal regulator of Akt signaling after activation; its phosphorylation by Akt, which is a prerequisite for ubiquitin ligase activity, suggests the existence of a regulation mechanism required to control Akt levels after activation. Catalyzes the formation of 'Lys-48'-polyubiquitin chains. May play a role in neuronal differentiation inhibition via its interaction with CIT.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1931 – 1971RING-type; atypicalPROSITE-ProRule annotationAdd BLAST41

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase TTC3 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase TTC3Curated
TPR repeat protein D
Short name:
Mtprd
Gene namesi
Name:Ttc3
Synonyms:Kiaa4119
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1276539 Ttc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003920341 – 1979E3 ubiquitin-protein ligase TTC3Add BLAST1979

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei378PhosphoserineBy similarity1
Modified residuei1009PhosphoserineCombined sources1
Modified residuei1060PhosphoserineCombined sources1
Modified residuei1794PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation on Ser-378 by Akt is required for ubiquitin ligase activity.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO88196
PaxDbiO88196
PeptideAtlasiO88196
PRIDEiO88196

PTM databases

iPTMnetiO88196
PhosphoSitePlusiO88196

Interactioni

Subunit structurei

Interacts (when phosphorylated on Ser-378) with AKT1, AKT2 and AKT3 (when phosphorylated). Interacts with CIT.By similarity

Protein-protein interaction databases

IntActiO88196, 4 interactors
MINTiO88196
STRINGi10090.ENSMUSP00000112801

Structurei

3D structure databases

ProteinModelPortaliO88196
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati231 – 264TPR 1Add BLAST34
Repeati266 – 298TPR 2Add BLAST33
Repeati536 – 572TPR 3Add BLAST37
Repeati576 – 609TPR 4Add BLAST34

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1018 – 1029Arg/Lys-rich (basic)Add BLAST12
Compositional biasi1171 – 1184Arg/Lys-rich (basic)Add BLAST14
Compositional biasi1547 – 1563Arg/Lys-rich (basic)Add BLAST17

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1931 – 1971RING-type; atypicalPROSITE-ProRule annotationAdd BLAST41

Keywords - Domaini

Repeat, TPR repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410JR26 Eukaryota
ENOG41103HK LUCA
HOVERGENiHBG007494
InParanoidiO88196
PhylomeDBiO88196

Family and domain databases

Gene3Di1.25.40.10, 2 hits
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00028 TPR, 4 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 3 hits
PS50293 TPR_REGION, 2 hits
PS50089 ZF_RING_2, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 25 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O88196-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDDFAEGGLS LADDILLEDY PYEDDCICTP DFTTDDYVRV TQLYYEGVGM
60 70 80 90 100
QYKDYAQSEK NLEYDICNIW CSKPLSILQD YCDAIKLYIF WPLLFQHQHS
110 120 130 140 150
SIISRLHPCV EAIRSRAAEI SLKKLQHLEL MEDIVDLAKK VANDSFLIEG
160 170 180 190 200
LLKIGYKIEN KILAMEDALN WIKYTGDVTI LPKLGSVDNC WPMLSIFFTE
210 220 230 240 250
YKYHITRVVT ENCNLLEEFR RHSCMQCVKQ GELMKMRGNE EFSKEKFEIA
260 270 280 290 300
VIYYTRAIEY RPENHLLYGN RALCFLRMGQ FRNALSDGKR AIVLKNTWPK
310 320 330 340 350
GHYRYCDALC MLGEYDWALQ ANIKAQKLCK NDPEGIKDLI QQHVKLQKQI
360 370 380 390 400
EDLQGRTSNK NPIKAFYESR AYIPRNSSAP AFRTSLNFVE TERGFRKTKY
410 420 430 440 450
KMANGGDQNQ KVADEALKGD DCDCHPEFLP PPSQPPRHKG KQKSRNNESE
460 470 480 490 500
KPSFNSEVSL QVDLKSILEK QFSKSSRAAH QDFANIMKML RSLIQDGYTA
510 520 530 540 550
LLEQRCRSAA QAFTELLNGL DPQKIKQLNL AMINYVLVVY GLAVSLLGIG
560 570 580 590 600
RPEELSEAEN QFKRIIEHYP NEGLDCLAYC GIGKVYLKKN RFLEALNHFE
610 620 630 640 650
KAKTLISRLP GILTWPTSNV IIEESKPEKV KVMLEKFVEE CKFPPVPDAV
660 670 680 690 700
CCYQKCRGYS KIQIYLTDPD FKGFIRISCC QYCKVEFHMN CWKKLKTTTF
710 720 730 740 750
NDKIDKDFLQ GICLTPDCEG IISKIIIYSS GGQVKCEFEH KVIKEKVPSR
760 770 780 790 800
PVLKQKCSSL EKLRLKEDKK LKRKIQKQEA KKLAQERMEE DLRESNPPKN
810 820 830 840 850
EEPEETSDSA QRCQFLDDRI LQCIKQNADK IKSVVLNTST LLKELLSWKV
860 870 880 890 900
LSTEDYTTCF SSKNFVHEAV DYVIGHLIQE KNRVKTRIFL HVLSELKELD
910 920 930 940 950
PKLAPWIQRL NSFGLDAIGP FFTRYGASLK ELDFHVVTVL WDEKYGHKLG
960 970 980 990 1000
SIEGKQLDYF FEPASAMEAR CLIWLLEEHR DKFPALHSAL DEFFDIMDSR
1010 1020 1030 1040 1050
CTVLRKQDSD EMPFGCIKVK NKGKKKKPKD SKPMLVGSGA ASVAPSSEAV
1060 1070 1080 1090 1100
TPEDHSRRNS DSAGPFAVPD HLRQDVEEFE ALYDQHSSEY VVRNKKLWDI
1110 1120 1130 1140 1150
NPKQKCSTLY DYFSQLLEEH GPLDMSDRMF SEEYEFFPEE TRQILEKAGG
1160 1170 1180 1190 1200
LKSFLLGCPR FVVIDNCIAL KKVASRLKKK RKKKNMKAKV EDISKTGEYL
1210 1220 1230 1240 1250
RVKLPLNPTA REFQPDVKSE ALSEDVKSIP GPADSSTLAA EDLKAQLDSD
1260 1270 1280 1290 1300
SSSGSASEDS RLEVASPDSP TPLCEDASPS PTPAPEEAKP TYWAQSHLVT
1310 1320 1330 1340 1350
GFCTYLPFQG FGITQRPAYI NMVPSLSQFT SIYTPLANIS SEYPMQRSMP
1360 1370 1380 1390 1400
VVPSFVASNR ADENAAAYFE SPNLNTEHDS GDHMASETQI LEDTLGVCVR
1410 1420 1430 1440 1450
SQGSAADADP ALSEPEGNSE HSGSSDSLWE ASLENVSGTT DAPAAPSVAI
1460 1470 1480 1490 1500
QVSRSMVHQE VNTEPYEPFE TQQGDLSQKE KECHLLREQL KVACENCEQI
1510 1520 1530 1540 1550
ELRSSQEIKD LEEKLQRHTE ENKISKTELD WFLQDLDREI KKWQQEKKEI
1560 1570 1580 1590 1600
QERLKALKKK IKKVINTSEM SAQKNDGFDK ECEPHPDQSL GFSSALTDEK
1610 1620 1630 1640 1650
TKAEESVRKG KELYEESHQR AVAAEVSVLE NWKEREVCKL QGVASQSEAY
1660 1670 1680 1690 1700
LKSLKLMSSD SATYPDVEYD ILSWESFLST VREEIESKKS QFEEHIKAIK
1710 1720 1730 1740 1750
NGSRLSDLSS VQLSEVSFPG CSTIHPQFLS ESSGHEDPGL VACVDSMTGA
1760 1770 1780 1790 1800
VLNDPYMDSA SGCPEEVPEL SLGSPTHQPE VTQQLELKGA SQVSPSEQSP
1810 1820 1830 1840 1850
EADEKLSGQA TRSSQSPKKP SNSIIEHLSV IFPCYTSTEL AGFIKKVRNK
1860 1870 1880 1890 1900
TKNSFSGLTI EEIVEKVTEH IVDEQKKKKP NPGKDKKTSE AHSAASVAKS
1910 1920 1930 1940 1950
SQSPPLAAAG PSARTKGQKK DDVPAPDGNS CEICHEIFKS KNMRVLKCGH
1960 1970
KFHKGCFKQW LKGQSTCPTC GSSDLLSEE
Length:1,979
Mass (Da):223,931
Last modified:March 2, 2010 - v2
Checksum:i63A93E3CA1CECF00
GO
Isoform 2 (identifier: O88196-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1056-1056: Missing.

Show »
Length:1,978
Mass (Da):223,844
Checksum:i6FEC4D995E55FCE4
GO
Isoform 3 (identifier: O88196-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     143-160: Missing.

Show »
Length:1,961
Mass (Da):221,883
Checksum:i4047B16D85EE30C5
GO
Isoform 4 (identifier: O88196-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     143-160: Missing.
     1056-1056: Missing.
     1089-1110: Missing.

Note: No experimental confirmation available.
Show »
Length:1,938
Mass (Da):219,088
Checksum:i58B90EF9CA3D66E2
GO
Isoform 5 (identifier: O88196-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-401: Missing.

Note: No experimental confirmation available.
Show »
Length:1,578
Mass (Da):177,071
Checksum:iD6D2B19BA5ADB72A
GO
Isoform 6 (identifier: O88196-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1593-1593: Missing.

Note: No experimental confirmation available.
Show »
Length:1,978
Mass (Da):223,844
Checksum:i620F3D1A57F9C9DA
GO

Computationally mapped potential isoform sequencesi

There are 25 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z485D3Z485_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
622Annotation score:
A0A0R4J1T2A0A0R4J1T2_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
776Annotation score:
D3Z502D3Z502_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
128Annotation score:
D3Z2K0D3Z2K0_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
382Annotation score:
G5E8T2G5E8T2_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3 mCG_19601
1,979Annotation score:
D6RDD2D6RDD2_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
1,162Annotation score:
D6RD03D6RD03_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
1,179Annotation score:
D6RHT6D6RHT6_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
1,135Annotation score:
D6RFU5D6RFU5_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
1,197Annotation score:
A0A338P6W3A0A338P6W3_MOUSE
E3 ubiquitin-protein ligase TTC3
Ttc3
1,624Annotation score:
There are more potential isoformsShow all

Sequence cautioni

The sequence AAH19173 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH57207 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAC35513 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAD90293 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE28586 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94L → P in BAA31563 (PubMed:9603993).Curated1
Sequence conflicti454F → S in BAC35513 (PubMed:9603993).Curated1
Sequence conflicti454F → S in BAE38412 (PubMed:9603993).Curated1
Sequence conflicti1164I → F in BAE37809 (PubMed:9603993).Curated1
Sequence conflicti1177L → P in BAC40250 (PubMed:9603993).Curated1
Sequence conflicti1522N → S in BAE36675 (PubMed:9603993).Curated1
Sequence conflicti1571S → P in BAE36675 (PubMed:9603993).Curated1
Sequence conflicti1571S → P in BAE37630 (PubMed:9603993).Curated1
Sequence conflicti1571S → P in BAE37809 (PubMed:9603993).Curated1
Sequence conflicti1603A → V in BAE36675 (PubMed:9603993).Curated1
Sequence conflicti1603A → V in BAE37630 (PubMed:9603993).Curated1
Sequence conflicti1603A → V in BAE37809 (PubMed:9603993).Curated1
Sequence conflicti1822N → S in BAE36675 (PubMed:9603993).Curated1
Sequence conflicti1847V → F in BAE36675 (PubMed:9603993).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0387741 – 401Missing in isoform 5. 1 PublicationAdd BLAST401
Alternative sequenceiVSP_038775143 – 160Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST18
Alternative sequenceiVSP_0387761056Missing in isoform 2 and isoform 4. 2 Publications1
Alternative sequenceiVSP_0387771089 – 1110Missing in isoform 4. 1 PublicationAdd BLAST22
Alternative sequenceiVSP_0387781593Missing in isoform 6. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008516 mRNA Translation: BAA31563.1
AK053765 mRNA Translation: BAC35513.1 Different initiation.
AK088273 mRNA Translation: BAC40250.1
AK162004 mRNA Translation: BAE36675.1
AK163496 mRNA Translation: BAE37372.1
AK164106 mRNA Translation: BAE37630.1
AK164492 mRNA Translation: BAE37809.1
AK165850 mRNA Translation: BAE38412.1
AK148494 mRNA Translation: BAE28586.1 Different initiation.
AK220495 mRNA Translation: BAD90293.1 Different initiation.
BC019173 mRNA Translation: AAH19173.1 Sequence problems.
BC057207 mRNA Translation: AAH57207.1 Sequence problems.
CCDSiCCDS37407.1 [O88196-1]
PIRiJW0059
UniGeneiMm.213408

Genome annotation databases

UCSCiuc008aau.1 mouse [O88196-2]
uc008aaw.1 mouse [O88196-3]
uc008aax.1 mouse [O88196-4]
uc012aiu.1 mouse [O88196-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008516 mRNA Translation: BAA31563.1
AK053765 mRNA Translation: BAC35513.1 Different initiation.
AK088273 mRNA Translation: BAC40250.1
AK162004 mRNA Translation: BAE36675.1
AK163496 mRNA Translation: BAE37372.1
AK164106 mRNA Translation: BAE37630.1
AK164492 mRNA Translation: BAE37809.1
AK165850 mRNA Translation: BAE38412.1
AK148494 mRNA Translation: BAE28586.1 Different initiation.
AK220495 mRNA Translation: BAD90293.1 Different initiation.
BC019173 mRNA Translation: AAH19173.1 Sequence problems.
BC057207 mRNA Translation: AAH57207.1 Sequence problems.
CCDSiCCDS37407.1 [O88196-1]
PIRiJW0059
UniGeneiMm.213408

3D structure databases

ProteinModelPortaliO88196
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO88196, 4 interactors
MINTiO88196
STRINGi10090.ENSMUSP00000112801

PTM databases

iPTMnetiO88196
PhosphoSitePlusiO88196

Proteomic databases

MaxQBiO88196
PaxDbiO88196
PeptideAtlasiO88196
PRIDEiO88196

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiuc008aau.1 mouse [O88196-2]
uc008aaw.1 mouse [O88196-3]
uc008aax.1 mouse [O88196-4]
uc012aiu.1 mouse [O88196-1]

Organism-specific databases

MGIiMGI:1276539 Ttc3
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410JR26 Eukaryota
ENOG41103HK LUCA
HOVERGENiHBG007494
InParanoidiO88196
PhylomeDBiO88196

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

ChiTaRSiTtc3 mouse
PROiPR:O88196
SOURCEiSearch...

Family and domain databases

Gene3Di1.25.40.10, 2 hits
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF13639 zf-RING_2, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00028 TPR, 4 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 3 hits
PS50293 TPR_REGION, 2 hits
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTTC3_MOUSE
AccessioniPrimary (citable) accession number: O88196
Secondary accession number(s): Q05D15
, Q3TMM9, Q3TPC8, Q3TPV5, Q3TQL0, Q3TSK0, Q3UFH5, Q5DTM5, Q6PG61, Q8BPM4, Q8C2N6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 2, 2010
Last sequence update: March 2, 2010
Last modified: October 10, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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