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Protein

Lantibiotic lacticin 3147 A1

Gene

ltnA1

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lanthionine-containing peptide antibiotic (lantibiotic) active on Gram-positive bacteria. The bactericidal activity of lantibiotics is based on depolarization of energized bacterial cytoplasmic membranes, initiated by the formation of aqueous transmembrane pores. When present individually lacticin 3147 A1 exhibits strong activity towards L.lactis strain AM2, weak activity towards L.lactis strain HP and no activity towards L.lactis strain IFPL359, but when combined with lacticin 3147 A2 it displays strong activity towards all three strains.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cytolysis Source: UniProtKB-KW
  • defense response to Gram-positive bacterium Source: UniProtKB

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Bacteriocin, Lantibiotic

Protein family/group databases

TCDBi1.C.21.2.4 the lacticin 481 (lacticin 481) family

Names & Taxonomyi

Protein namesi
Recommended name:
Lantibiotic lacticin 3147 A1
Gene namesi
Name:ltnA11 Publication
Synonyms:ltnAImported
ORF Names:ORF00035
Encoded oniPlasmid pMRC01Imported
Plasmid pBAC105Imported
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000428491 – 292 PublicationsAdd BLAST29
PeptideiPRO_000004285030 – 59Lantibiotic lacticin 3147 A11 PublicationAdd BLAST30

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki30 ↔ 31Lanthionine (Cys-Ser)1 Publication
Modified residuei322,3-didehydrobutyrine1 Publication1
Modified residuei342,3-didehydrobutyrine1 Publication1
Modified residuei362,3-didehydroalanine (Ser)1 Publication1
Cross-linki38 ↔ 48Lanthionine (Ser-Cys)1 Publication
Cross-linki49 ↔ 54Beta-methyllanthionine (Thr-Cys)1 Publication
Cross-linki51 ↔ 58Beta-methyllanthionine (Thr-Cys)1 Publication

Post-translational modificationi

Maturation of lantibiotics involves the enzymic conversion of Thr, and Ser into dehydrated AA and the formation of thioether bonds with cysteine. This is followed by membrane translocation and cleavage of the modified precursor.1 Publication
It is not established whether the 2,3-didehydrobutyrines are the E- or Z-isomers (PubMed:15023056 and PubMed:10608807). In the NMR model they were assumed to be the Z-isomer.

Keywords - PTMi

Thioether bond

Interactioni

GO - Molecular functioni

Structurei

3D structure databases

SMRiO87236
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Family and domain databases

InterProiView protein in InterPro
IPR029243 Lantibiotic_alpha
PfamiView protein in Pfam
PF14867 Lantibiotic_a, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O87236-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNKNEIETQP VTWLEEVSDQ NFDEDVFGAC STNTFSLSDY WGNNGAWCTL

THECMAWCK
Length:59
Mass (Da):6,798
Last modified:November 1, 1998 - v1
Checksum:i850B313769976D42
GO

Mass spectrometryi

Molecular mass is 3322 Da from positions 30 - 59. Determined by PD. 1 Publication
Molecular mass is 3306.69 Da from positions 30 - 59. Determined by MALDI. 1 Publication
Molecular mass is 3322.34 Da from positions 30 - 59. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001272 Genomic DNA Translation: AAC56053.1
AF167432 Genomic DNA Translation: AAF32256.1
PIRiT43106
RefSeqiNP_047319.1, NC_001949.1

Genome annotation databases

GeneIDi1113495

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001272 Genomic DNA Translation: AAC56053.1
AF167432 Genomic DNA Translation: AAF32256.1
PIRiT43106
RefSeqiNP_047319.1, NC_001949.1

3D structure databases

SMRiO87236
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.21.2.4 the lacticin 481 (lacticin 481) family

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1113495

Family and domain databases

InterProiView protein in InterPro
IPR029243 Lantibiotic_alpha
PfamiView protein in Pfam
PF14867 Lantibiotic_a, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLANA1_LACLL
AccessioniPrimary (citable) accession number: O87236
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 1, 1998
Last modified: May 23, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing, Plasmid
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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