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Protein

Maleylpyruvate isomerase

Gene

nagL

Organism
Ralstonia sp.
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the GSH-dependent isomerization of maleylpyruvate to fumarylpyruvate which is subsequently processed by NagK to form pyruvate and fumarate.1 Publication

Catalytic activityi

3-maleylpyruvate = 3-fumarylpyruvate.1 Publication

Cofactori

glutathione1 Publication

Pathwayi: naphthalene degradation

This protein is involved in the pathway naphthalene degradation, which is part of Aromatic compound metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway naphthalene degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Glutathione1 Publication1
Binding sitei52Glutathione; via amide nitrogen and carbonyl oxygen1 Publication1
Binding sitei176Glutathione1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processAromatic hydrocarbons catabolism
LigandPyruvate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14770
UniPathwayi
UPA00082

Names & Taxonomyi

Protein namesi
Recommended name:
Maleylpyruvate isomerase1 PublicationImported (EC:5.2.1.41 Publication)
Short name:
MPI1 Publication
Alternative name(s):
Naphthalene degradation protein L
Gene namesi
Name:nagLImported
Encoded oniPlasmid pWWU2Imported
OrganismiRalstonia sp.
Taxonomic identifieri54061 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004214691 – 212Maleylpyruvate isomeraseAdd BLAST212

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

Secondary structure

1212
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO86043
SMRiO86043
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO86043

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 80GST N-terminalSequence analysisAdd BLAST80
Domaini85 – 212GST C-terminalSequence analysisAdd BLAST128

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni9 – 11Glutathione binding1 Publication3
Regioni64 – 65Glutathione binding1 Publication2
Regioni102 – 104Glutathione binding1 Publication3
Regioni108 – 110Glutathione binding1 Publication3

Sequence similaritiesi

Belongs to the GST superfamily. Zeta family.Sequence analysis

Family and domain databases

CDDicd03191 GST_C_Zeta, 1 hit
cd03042 GST_N_Zeta, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR005955 GST_Zeta
IPR034330 GST_Zeta_C
IPR034333 GST_Zeta_N
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR01262 maiA, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

O86043-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKLYNFWRSG TSHRLRIALN LKGVPYEYLA VHLGKEEHLK DAFKALNPQQ
60 70 80 90 100
LVPALDTGAQ VLIQSPAIIE WLEEQYPTPA LLPADADGRQ RVRALAAIVG
110 120 130 140 150
CDIHPINNRR ILEYLRKTFG ADEAAINAWC GTWISAGFDA YEALLAVDPK
160 170 180 190 200
RGRYSFGDTP TLADCYLVPQ VESARRFQVD LTPYPLIRAV DAACGELDAF
210
RRAAPAAQPD SA
Length:212
Mass (Da):23,505
Last modified:November 1, 1998 - v1
Checksum:i6554F3B95591EAEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036940 Genomic DNA Translation: AAD12621.1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036940 Genomic DNA Translation: AAD12621.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2JL4X-ray2.30A/B1-212[»]
2V6KX-ray1.30A/B1-212[»]
ProteinModelPortaliO86043
SMRiO86043
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayi
UPA00082

BioCyciMetaCyc:MONOMER-14770

Miscellaneous databases

EvolutionaryTraceiO86043

Family and domain databases

CDDicd03191 GST_C_Zeta, 1 hit
cd03042 GST_N_Zeta, 1 hit
InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR004045 Glutathione_S-Trfase_N
IPR005955 GST_Zeta
IPR034330 GST_Zeta_C
IPR034333 GST_Zeta_N
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF13417 GST_N_3, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR01262 maiA, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNAGL_RALSP
AccessioniPrimary (citable) accession number: O86043
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: November 1, 1998
Last modified: October 25, 2017
This is version 76 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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