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UniProtKB - O86028 (MCM_RHIME)
Protein
Methylmalonyl-CoA mutase
Gene
bhbA
Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Functioni
Radical enzyme that catalyzes the transformation of methylmalonyl-CoA to succinyl-CoA. Is required for growth on the polyhydroxyalkanoate degradation pathway intermediates 3-hydroxybutyrate and acetoacetate as sole carbon source.
2 PublicationsCatalytic activityi
- EC:5.4.99.21 Publication1 Publication
Cofactori
Protein has several cofactor binding sites:- adenosylcob(III)alamin1 Publication
- a monovalent cation1 PublicationNote: Monovalent cations such as NH4(+), Rb+, Cs+, K+, Li+ or Na+ are required for enzyme activity.1 Publication
Kineticsi
- KM=0.11 mM for (R,S)-methylmalonyl-CoA (in the presence of 3 mM NH4+)1 Publication
- KM=0.13 mM for (R,S)-methylmalonyl-CoA (in the absence of 3 mM NH4+)1 Publication
pH dependencei
Optimum pH is 7.0.1 Publication
Temperature dependencei
Optimum temperature is 37 degrees Celsius.1 Publication
: propanoyl-CoA degradation Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes succinyl-CoA from propanoyl-CoA.Curated This subpathway is part of the pathway propanoyl-CoA degradation, which is itself part of Metabolic intermediate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinyl-CoA from propanoyl-CoA, the pathway propanoyl-CoA degradation and in Metabolic intermediate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 195 | SubstrateBy similarity | 1 | |
Binding sitei | 222 | SubstrateBy similarity | 1 | |
Binding sitei | 232 | SubstrateBy similarity | 1 | |
Metal bindingi | 593 | Cobalt (cobalamin axial ligand)By similarity | 1 |
GO - Molecular functioni
- cobalamin binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- methylmalonyl-CoA mutase activity Source: UniProtKB-EC
Keywordsi
Molecular function | Isomerase |
Ligand | Cobalamin, Cobalt, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00945;UER00910 |
Names & Taxonomyi
Protein namesi | Recommended name: Methylmalonyl-CoA mutase2 Publications (EC:5.4.99.21 Publication1 Publication)Short name: MCM1 Publication |
Gene namesi | Name:bhbA1 Publication Ordered Locus Names:RB1449 ORF Names:SMb20757 |
Encoded oni | Plasmid pSymB (megaplasmid 2) |
Organismi | Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) |
Taxonomic identifieri | 266834 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Hyphomicrobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Cells lacking this gene show no methylmalonyl-CoA mutase activity, in contrast to wild-type.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000194274 | 2 – 712 | Methylmalonyl-CoA mutaseAdd BLAST | 711 |
Interactioni
Subunit structurei
Homodimer.
1 Publication1 PublicationProtein-protein interaction databases
STRINGi | 266834.SM_b20757 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 580 – 712 | B12-bindingPROSITE-ProRule annotationAdd BLAST | 133 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 73 – 77 | Substrate bindingBy similarity | 5 | |
Regioni | 183 – 185 | Substrate bindingBy similarity | 3 | |
Regioni | 271 – 273 | Substrate bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the methylmalonyl-CoA mutase family.Curated
Phylogenomic databases
eggNOGi | COG1884, Bacteria COG2185, Bacteria |
HOGENOMi | CLU_009523_3_1_5 |
OMAi | IAQKEWV |
Family and domain databases
InterProi | View protein in InterPro IPR006159, Acid_CoA_mut_C IPR016176, Cbl-dep_enz_cat IPR006158, Cobalamin-bd IPR036724, Cobalamin-bd_sf IPR006099, MeMalonylCoA_mutase_a/b_cat IPR006098, MMCoA_mutase_a_cat |
Pfami | View protein in Pfam PF02310, B12-binding, 1 hit PF01642, MM_CoA_mutase, 1 hit |
SUPFAMi | SSF51703, SSF51703, 1 hit SSF52242, SSF52242, 1 hit |
TIGRFAMsi | TIGR00640, acid_CoA_mut_C, 1 hit TIGR00641, acid_CoA_mut_N, 1 hit |
PROSITEi | View protein in PROSITE PS51332, B12_BINDING, 1 hit PS00544, METMALONYL_COA_MUTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O86028-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEKTIKDWE ALAEKELRVS PEGLVWHTPE GIDVKPLYTS DDMSGIGHLN
60 70 80 90 100
SLPGFEPFVR GPRATMYAGR PWTVRQYAGF STAEASNAFY RRNLAAGQQG
110 120 130 140 150
VSVAFDLATH RGYDSDHPRV QGDVGKAGVA IDSVEDMKIL FDGIPLDRIS
160 170 180 190 200
VSMTMNGAVI PILASFIVAG EEQGVSRDKL SGTIQNDILK EFMVRNTYIY
210 220 230 240 250
PPEPSMRIVA DIIEYTAKEM PKFNSISISG YHMQEAGATL VQELAFTLAD
260 270 280 290 300
GREYVRAALA KGLNVDDFAG RLSFFFAIGM NFFMEAAKLR AARLLWTRIM
310 320 330 340 350
QEFKPEKASS LMLRTHCQTS GVSLQEQDPY NNIVRTAFEA MSAVLGGTQS
360 370 380 390 400
LHTNSFDEAM ALPTDFSARI ARNTQLILQH ETGVTKVVDP LAGSYYVESL
410 420 430 440 450
TNELAEKAWG LIEEVEALGG MTKAVNAGLP KRLIEEAATR RQAAVDRAEE
460 470 480 490 500
VIVGVNKYRL ENEQPIDILQ IDNAAVRTAQ VKRIEETRRR RDSQKMKQAL
510 520 530 540 550
DALADVARSG KGNLLAAAVE AARARATVGE ITDAMREAFG DYTAIPEVVT
560 570 580 590 600
DIYGKAYEGD PELGVLAGRL GEATKRLGHK PKIMVAKLGQ DGHDRGAKVI
610 620 630 640 650
ASAFGDIGFD VVAGPLFQTP EEAADLALAE EVTVIGVSSL AAGHRTLMPQ
660 670 680 690 700
LAEALKKRGG EDIIVVCGGV IPRQDYDYLM ENGVAAVFGP GTQVLDAARA
710
VLDLIEGKRR NV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 47 | G → R in AAD13665 (PubMed:9889346).Curated | 1 | |
Sequence conflicti | 61 | G → R in AAD13665 (PubMed:9889346).Curated | 1 | |
Sequence conflicti | 156 | N → K in AAD13665 (PubMed:9889346).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF080073 Genomic DNA Translation: AAD13665.1 AL591985 Genomic DNA Translation: CAC49849.1 |
PIRi | A96023 |
RefSeqi | NP_437989.1, NC_003078.1 WP_010976251.1, NC_003078.1 |
Genome annotation databases
EnsemblBacteriai | CAC49849; CAC49849; SM_b20757 |
GeneIDi | 61601358 |
KEGGi | sme:SM_b20757 |
PATRICi | fig|266834.11.peg.6375 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF080073 Genomic DNA Translation: AAD13665.1 AL591985 Genomic DNA Translation: CAC49849.1 |
PIRi | A96023 |
RefSeqi | NP_437989.1, NC_003078.1 WP_010976251.1, NC_003078.1 |
3D structure databases
SMRi | O86028 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 266834.SM_b20757 |
Genome annotation databases
EnsemblBacteriai | CAC49849; CAC49849; SM_b20757 |
GeneIDi | 61601358 |
KEGGi | sme:SM_b20757 |
PATRICi | fig|266834.11.peg.6375 |
Phylogenomic databases
eggNOGi | COG1884, Bacteria COG2185, Bacteria |
HOGENOMi | CLU_009523_3_1_5 |
OMAi | IAQKEWV |
Enzyme and pathway databases
UniPathwayi | UPA00945;UER00910 |
Family and domain databases
InterProi | View protein in InterPro IPR006159, Acid_CoA_mut_C IPR016176, Cbl-dep_enz_cat IPR006158, Cobalamin-bd IPR036724, Cobalamin-bd_sf IPR006099, MeMalonylCoA_mutase_a/b_cat IPR006098, MMCoA_mutase_a_cat |
Pfami | View protein in Pfam PF02310, B12-binding, 1 hit PF01642, MM_CoA_mutase, 1 hit |
SUPFAMi | SSF51703, SSF51703, 1 hit SSF52242, SSF52242, 1 hit |
TIGRFAMsi | TIGR00640, acid_CoA_mut_C, 1 hit TIGR00641, acid_CoA_mut_N, 1 hit |
PROSITEi | View protein in PROSITE PS51332, B12_BINDING, 1 hit PS00544, METMALONYL_COA_MUTASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MCM_RHIME | |
Accessioni | O86028Primary (citable) accession number: O86028 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | October 24, 2001 | |
Last modified: | February 23, 2022 | |
This is version 131 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Plasmid, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families