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UniProtKB - O85465 (GUN5_SALAG)
Protein
Endoglucanase 5A
Gene
cel5A
Organism
Salipaludibacillus agaradhaerens (Bacillus agaradhaerens)
Status
Functioni
Catalytic activityi
- Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.2 Publications EC:3.2.1.4
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 61 | SubstrateCombined sources3 Publications | 1 | |
Binding sitei | 92 | SubstrateCombined sources3 Publications | 1 | |
Binding sitei | 127 | SubstrateCombined sources3 Publications | 1 | |
Active sitei | 165 | Proton donor4 Publications | 1 | |
Binding sitei | 228 | SubstrateCombined sources2 Publications | 1 | |
Active sitei | 254 | NucleophileCombined sources4 Publications | 1 | |
Binding sitei | 288 | SubstrateCombined sources4 Publications | 1 |
GO - Molecular functioni
- carbohydrate binding Source: InterPro
- cellulase activity Source: UniProtKB-EC
GO - Biological processi
- cellulose catabolic process Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Biological process | Carbohydrate metabolism, Cellulose degradation, Polysaccharide degradation |
Protein family/group databases
CAZyi | CBM5, Carbohydrate-Binding Module Family 5 GH5, Glycoside Hydrolase Family 5 |
Names & Taxonomyi
Protein namesi | Recommended name: Endoglucanase 5A (EC:3.2.1.42 Publications)Alternative name(s): Alkaline cellulase Endo-1,4-beta-glucanase 5A |
Gene namesi | Name:cel5A |
Organismi | Salipaludibacillus agaradhaerens (Bacillus agaradhaerens) |
Taxonomic identifieri | 76935 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Chemistry databases
DrugBanki | DB04086, 2',4'-Dinitrophenyl-2deoxy-2-Fluro-B-D-Cellobioside DB03584, 4-Thio-beta-D-glucopyranose DB04545, Afegostat DB02379, Beta-D-Glucose DB02061, Cellobiose DB01633, Deoxy-2-fluoro-beta-D-cellotrioside DB02017, Imidazole-Derived Cellobiose DB01642, methyl beta-D-glucopyranoside DB03862, Tetrahydrooxazine |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 26 | 1 PublicationAdd BLAST | 26 | |
ChainiPRO_0000184043 | 27 – 400 | Endoglucanase 5AAdd BLAST | 374 |
Interactioni
Subunit structurei
Monomer.
1 PublicationStructurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | O85465 |
SMRi | O85465 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O85465 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 357 – 396 | Chitin-binding type-3Sequence analysisAdd BLAST | 40 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 65 – 66 | Substrate bindingCombined sources3 Publications | 2 | |
Regioni | 260 – 261 | Substrate bindingCombined sources2 Publications | 2 | |
Regioni | 293 – 295 | Substrate bindingCombined sources3 Publications | 3 | |
Regioni | 328 – 363 | DisorderedSequence analysisAdd BLAST | 36 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 333 – 357 | Pro residuesSequence analysisAdd BLAST | 25 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 5 (cellulase A) family.Curated
Keywords - Domaini
SignalFamily and domain databases
InterProi | View protein in InterPro IPR003610, CBM_fam5/12 IPR036573, CBM_sf_5/12 IPR001547, Glyco_hydro_5 IPR018087, Glyco_hydro_5_CS IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00150, Cellulase, 1 hit |
SMARTi | View protein in SMART SM00495, ChtBD3, 1 hit |
SUPFAMi | SSF51055, SSF51055, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00659, GLYCOSYL_HYDROL_F5, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O85465-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKKITTIFVV LLMTVALFSI GNTTAADNDS VVEEHGQLSI SNGELVNERG
60 70 80 90 100
EQVQLKGMSS HGLQWYGQFV NYESMKWLRD DWGINVFRAA MYTSSGGYID
110 120 130 140 150
DPSVKEKVKE AVEAAIDLDI YVIIDWHILS DNDPNIYKEE AKDFFDEMSE
160 170 180 190 200
LYGDYPNVIY EIANEPNGSD VTWGNQIKPY AEEVIPIIRN NDPNNIIIVG
210 220 230 240 250
TGTWSQDVHH AADNQLADPN VMYAFHFYAG THGQNLRDQV DYALDQGAAI
260 270 280 290 300
FVSEWGTSAA TGDGGVFLDE AQVWIDFMDE RNLSWANWSL THKDESSAAL
310 320 330 340 350
MPGANPTGGW TEAELSPSGT FVREKIRESA SIPPSDPTPP SDPGEPDPTP
360 370 380 390 400
PSDPGEYPAW DPNQIYTNEI VYHNGQLWQA KWWTQNQEPG DPYGPWEPLN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF067428 Genomic DNA Translation: AAC19169.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF067428 Genomic DNA Translation: AAC19169.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1A3H | X-ray | 1.57 | A | 30-329 | [»] | |
1E5J | X-ray | 1.85 | A | 27-331 | [»] | |
1H11 | X-ray | 1.08 | A | 27-329 | [»] | |
1H2J | X-ray | 1.15 | A | 27-329 | [»] | |
1H5V | X-ray | 1.10 | A | 27-330 | [»] | |
1HF6 | X-ray | 1.15 | A | 27-329 | [»] | |
1OCQ | X-ray | 1.08 | A | 27-329 | [»] | |
1QHZ | X-ray | 1.95 | A | 27-331 | [»] | |
1QI0 | X-ray | 2.10 | A | 27-331 | [»] | |
1QI2 | X-ray | 1.75 | A | 27-331 | [»] | |
1W3K | X-ray | 1.20 | A | 27-329 | [»] | |
1W3L | X-ray | 1.04 | A | 27-329 | [»] | |
2A3H | X-ray | 2.00 | A | 30-329 | [»] | |
2V38 | X-ray | 1.50 | A | 27-331 | [»] | |
3A3H | X-ray | 1.64 | A | 30-329 | [»] | |
4A3H | X-ray | 1.65 | A | 27-329 | [»] | |
5A3H | X-ray | 1.82 | A | 27-329 | [»] | |
6A3H | X-ray | 1.68 | A | 27-329 | [»] | |
7A3H | X-ray | 0.95 | A | 27-329 | [»] | |
8A3H | X-ray | 0.97 | A | 27-329 | [»] | |
AlphaFoldDBi | O85465 | |||||
SMRi | O85465 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Chemistry databases
DrugBanki | DB04086, 2',4'-Dinitrophenyl-2deoxy-2-Fluro-B-D-Cellobioside DB03584, 4-Thio-beta-D-glucopyranose DB04545, Afegostat DB02379, Beta-D-Glucose DB02061, Cellobiose DB01633, Deoxy-2-fluoro-beta-D-cellotrioside DB02017, Imidazole-Derived Cellobiose DB01642, methyl beta-D-glucopyranoside DB03862, Tetrahydrooxazine |
Protein family/group databases
CAZyi | CBM5, Carbohydrate-Binding Module Family 5 GH5, Glycoside Hydrolase Family 5 |
Miscellaneous databases
EvolutionaryTracei | O85465 |
Family and domain databases
InterProi | View protein in InterPro IPR003610, CBM_fam5/12 IPR036573, CBM_sf_5/12 IPR001547, Glyco_hydro_5 IPR018087, Glyco_hydro_5_CS IPR017853, Glycoside_hydrolase_SF |
Pfami | View protein in Pfam PF00150, Cellulase, 1 hit |
SMARTi | View protein in SMART SM00495, ChtBD3, 1 hit |
SUPFAMi | SSF51055, SSF51055, 1 hit SSF51445, SSF51445, 1 hit |
PROSITEi | View protein in PROSITE PS00659, GLYCOSYL_HYDROL_F5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GUN5_SALAG | |
Accessioni | O85465Primary (citable) accession number: O85465 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families