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Protein

Argininosuccinate synthase

Gene

argG

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87CitrullineUniRule annotation1
Binding sitei117ATP; via amide nitrogenUniRule annotation1
Binding sitei119AspartateUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei123CitrullineUniRule annotation1
Binding sitei124AspartateUniRule annotation1
Binding sitei127CitrullineUniRule annotation1
Binding sitei175CitrullineUniRule annotation1
Binding sitei259CitrullineUniRule annotation1
Binding sitei271CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 16ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processAmino-acid biosynthesis, Arginine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCORYNE:G18NG-10979-MONOMER
UniPathwayiUPA00068; UER00113

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Cgl1400, cg1586
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000000582 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485901 – 401Argininosuccinate synthaseAdd BLAST401

2D gel databases

World-2DPAGEi0001:O85176

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi196627.cg1586

Structurei

3D structure databases

ProteinModelPortaliO85176
SMRiO85176
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH Bacteria
COG0137 LUCA
HOGENOMiHOG000230093
KOiK01940
OMAiGVGRIDM

Family and domain databases

CDDicd01999 Argininosuccinate_Synthase, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.1260.10, 2 hits
HAMAPiMF_00005 Arg_succ_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001518 Arginosuc_synth
IPR018223 Arginosuc_synth_CS
IPR023434 Arginosuc_synth_type_1_subfam
IPR024074 AS_cat/multimer_dom_body
IPR014729 Rossmann-like_a/b/a_fold
PANTHERiPTHR11587 PTHR11587, 1 hit
PfamiView protein in Pfam
PF00764 Arginosuc_synth, 1 hit
TIGRFAMsiTIGR00032 argG, 1 hit
PROSITEiView protein in PROSITE
PS00564 ARGININOSUCCIN_SYN_1, 1 hit
PS00565 ARGININOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

O85176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTNRIVLAYS GGLDTTVAIP YLKKMIDGEV IAVSLDLGQG GENMDNVRQR
60 70 80 90 100
ALDAGAAESI VVDAKDEFAE EYCLPTIKAN GMYMKQYPLV SAISRPLIVK
110 120 130 140 150
HLVEAGKQFN GTHVAHGCTG KGNDQVRFEV GFMDTDPNLE IIAPARDFAW
160 170 180 190 200
TRDKAIAFAE ENNVPIEQSV KSPFSIDQNV WGRAIETGYL EDLWNAPTKD
210 220 230 240 250
IYAYTEDPAL GNAPDEVIIS FEGGKPVSID GRPVSVLQAI EELNRRAGAQ
260 270 280 290 300
GVGRLDMVED RLVGIKSREI YEAPGAIALI KAHEALEDVT IERELARYKR
310 320 330 340 350
GVDARWAEEV YDGLWFGPLK RSLDAFIDST QEHVTGDIRM VLHAGSITIN
360 370 380 390 400
GRRSSHSLYD FNLATYDTGD TFDQTLAKGF VQLHGLSSKI ANKRDREAGN

N
Length:401
Mass (Da):44,135
Last modified:July 11, 2002 - v2
Checksum:i271C63DD9C50CDFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti127R → G in AAC24818 (Ref. 1) Curated1
Sequence conflicti304A → R in AAC24818 (Ref. 1) Curated1
Sequence conflicti304A → R in AAB86624 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049897 Genomic DNA Translation: AAC24818.1
AF030520 Genomic DNA Translation: AAB86624.1
BA000036 Genomic DNA Translation: BAB98793.1
BX927152 Genomic DNA Translation: CAF21411.1
RefSeqiNP_600619.1, NC_003450.3
WP_003858678.1, NC_006958.1

Genome annotation databases

EnsemblBacteriaiBAB98793; BAB98793; BAB98793
CAF21411; CAF21411; cg1586
GeneIDi1019376
KEGGicgb:cg1586
cgl:NCgl1346
PATRICifig|196627.13.peg.1369

Similar proteinsi

Entry informationi

Entry nameiASSY_CORGL
AccessioniPrimary (citable) accession number: O85176
Secondary accession number(s): O31144
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 11, 2002
Last modified: May 23, 2018
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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