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Entry version 148 (02 Jun 2021)
Sequence version 1 (01 Nov 1998)
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Protein

DNA topoisomerase 1

Gene

topA

Organism
Chlamydia trachomatis (strain D/UW-3/Cx)
Status
Unreviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.

UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

  • ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationARBA annotation EC:5.6.2.1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei315O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-bindingUniRule annotationARBA annotation, Isomerase, TopoisomeraseUniRule annotationARBA annotation
LigandMagnesiumARBA annotation, Metal-binding, Zinc

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.6.2.1UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:topAUniRule annotationImported
Ordered Locus Names:CT_643Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiChlamydia trachomatis (strain D/UW-3/Cx)Imported
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri272561 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaChlamydiaeChlamydialesChlamydiaceaeChlamydia/Chlamydophila groupChlamydia
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000431 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Monomer.

UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei33Interaction with DNAUniRule annotation1
Sitei138Interaction with DNAUniRule annotation1
Sitei139Interaction with DNAUniRule annotation1
Sitei142Interaction with DNAUniRule annotation1
Sitei147Interaction with DNAUniRule annotation1
Sitei317Interaction with DNAUniRule annotation1
Sitei509Interaction with DNAUniRule annotation1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini3 – 112ToprimInterPro annotationAdd BLAST110

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni164 – 169Interaction with DNAUniRule annotation6

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationARBA annotation

Keywords - Domaini

Zinc-fingerARBA annotation

Phylogenomic databases

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_002929_4_3_0

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O84649

Identification of Orthologs from Complete Genome Data

More...
OMAi
PECKYTR

Family and domain databases

Conserved Domains Database

More...
CDDi
cd00186, TOP1Ac, 1 hit
cd03363, TOPRIM_TopoIA_TopoI, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.245.10, 1 hit
1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00952, Topoisom_1_prok, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR019835, SWIB_domain
IPR036885, SWIB_MDM2_dom_sf
IPR003121, SWIB_MDM2_domain
IPR000380, Topo_IA
IPR003601, Topo_IA_2
IPR023406, Topo_IA_AS
IPR013497, Topo_IA_cen
IPR013824, Topo_IA_cen_sub1
IPR013825, Topo_IA_cen_sub2
IPR013826, Topo_IA_cen_sub3
IPR023405, Topo_IA_core_domain
IPR003602, Topo_IA_DNA-bd_dom
IPR013498, Topo_IA_Znf
IPR005733, TopoI_bac-type
IPR028612, Topoisom_1_IA
IPR006171, TOPRIM_domain
IPR034149, TOPRIM_TopoI

The PANTHER Classification System

More...
PANTHERi
PTHR42785, PTHR42785, 2 hits

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF02201, SWIB, 1 hit
PF01131, Topoisom_bac, 1 hit
PF01751, Toprim, 1 hit
PF01396, zf-C4_Topoisom, 3 hits

Protein Motif fingerprint database; a protein domain database

More...
PRINTSi
PR00417, PRTPISMRASEI

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00151, SWIB, 1 hit
SM00437, TOP1Ac, 1 hit
SM00436, TOP1Bc, 1 hit
SM00493, TOPRIM, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF47592, SSF47592, 1 hit
SSF56712, SSF56712, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR01051, topA_bact, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00396, TOPOISOMERASE_I_PROK, 1 hit
PS50880, TOPRIM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O84649-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKKSLIIVES PAKIKTLRKL LGEGFIFDSS LGHIVDLPAK GFGIDIENGF
60 70 80 90 100
VPDYQILEGK KEVIRKICAE AKKCDVVYLA PDPDREGEAI AWHIANQLPK
110 120 130 140 150
DTKIQRISFN AITKGAVTEA LKHPREIDMA LVNAQQARRF LDRIVGYKIS
160 170 180 190 200
PILGRKLQRW SGVSAGRVQS VALKLVVDRE YAIERFVPVE FWNIRVHLKD
210 220 230 240 250
PQTQKTFWAH LHSVNGKKWE KEIPEGKTSD EVILIDSKEK ADEIVALLES
260 270 280 290 300
ATYVVDRVES KEKKRHAYPP FITSTLQQEA SRHYRFSSSR TMNIAQTLYE
310 320 330 340 350
GVDLDSQGAV GLITYMRTDS VRTDPEAVKQ VRKYIEGHFG KEFVPSSPNV
360 370 380 390 400
YATKKMAQDA HEAIRPTDVT ITPESIRSKL TEDQYKLYSL IWKRFVASQM
410 420 430 440 450
ISAIYDTLAI RITTNKGIDL RATGSCLKFK GFLAVYEEKR DEEGDEEENI
460 470 480 490 500
HLPKLNERDV LTKEELEAEQ SHTKPLPRFT EASLVKELEK SGIGRPSTYA
510 520 530 540 550
TIMNKIQSRE YTLKEGQRLR PTELGKVVCQ FLETNFPRIM DIGFTAGMED
560 570 580 590 600
ELELIADNKK PWKQLLQEFC ELFLPFVVTA EKEAFIPRIV TEIDCPKCHK
610 620 630 640 650
GKLVKIWAKN RYFFGCSEYP TCDYKTSEEE LTFDKNEYAE DTPWDAPCAL
660 670 680 690 700
CGGEMKVRHG KFGSFLGCEN YPKCHYIVNL FKKGEAGAEP EATVHCPAEG
710 720 730 740 750
CTGHLVKRRS RFNKMFYSCS EYPACSVIGN SVDAVIEKYA GTPKTPYEKK
760 770 780 790 800
PKAKKSIAST KGKAAKTVKK SSATTKKRAT KAYTPSAALA AVIGADPVGR
810 820 830 840 850
PEATKKLWEY IKEKGLQSPQ NKKIIIPDSK LQGVIGADPI DMFALSKKLS

AHLIKEE
Length:857
Mass (Da):96,704
Last modified:November 1, 1998 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5ABD780B5DBBB562
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AE001273 Genomic DNA Translation: AAC68820.1

Protein sequence database of the Protein Information Resource

More...
PIRi
A71488

NCBI Reference Sequences

More...
RefSeqi
NP_220161.1, NC_000117.1
WP_010725288.1, NC_000117.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC68820; AAC68820; CT_643

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
884426

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ctr:CT_643

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|272561.5.peg.707

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001273 Genomic DNA Translation: AAC68820.1
PIRiA71488
RefSeqiNP_220161.1, NC_000117.1
WP_010725288.1, NC_000117.1

3D structure databases

Database of comparative protein structure models

More...
ModBasei
Search...

SWISS-MODEL Interactive Workspace

More...
SWISS-MODEL-Workspacei
Submit a new modelling project...

Genome annotation databases

EnsemblBacteriaiAAC68820; AAC68820; CT_643
GeneIDi884426
KEGGictr:CT_643
PATRICifig|272561.5.peg.707

Phylogenomic databases

HOGENOMiCLU_002929_4_3_0
InParanoidiO84649
OMAiPECKYTR

Family and domain databases

CDDicd00186, TOP1Ac, 1 hit
cd03363, TOPRIM_TopoIA_TopoI, 1 hit
Gene3Di1.10.245.10, 1 hit
1.10.290.10, 1 hit
1.10.460.10, 1 hit
2.70.20.10, 1 hit
HAMAPiMF_00952, Topoisom_1_prok, 1 hit
InterProiView protein in InterPro
IPR019835, SWIB_domain
IPR036885, SWIB_MDM2_dom_sf
IPR003121, SWIB_MDM2_domain
IPR000380, Topo_IA
IPR003601, Topo_IA_2
IPR023406, Topo_IA_AS
IPR013497, Topo_IA_cen
IPR013824, Topo_IA_cen_sub1
IPR013825, Topo_IA_cen_sub2
IPR013826, Topo_IA_cen_sub3
IPR023405, Topo_IA_core_domain
IPR003602, Topo_IA_DNA-bd_dom
IPR013498, Topo_IA_Znf
IPR005733, TopoI_bac-type
IPR028612, Topoisom_1_IA
IPR006171, TOPRIM_domain
IPR034149, TOPRIM_TopoI
PANTHERiPTHR42785, PTHR42785, 2 hits
PfamiView protein in Pfam
PF02201, SWIB, 1 hit
PF01131, Topoisom_bac, 1 hit
PF01751, Toprim, 1 hit
PF01396, zf-C4_Topoisom, 3 hits
PRINTSiPR00417, PRTPISMRASEI
SMARTiView protein in SMART
SM00151, SWIB, 1 hit
SM00437, TOP1Ac, 1 hit
SM00436, TOP1Bc, 1 hit
SM00493, TOPRIM, 1 hit
SUPFAMiSSF47592, SSF47592, 1 hit
SSF56712, SSF56712, 1 hit
TIGRFAMsiTIGR01051, topA_bact, 1 hit
PROSITEiView protein in PROSITE
PS00396, TOPOISOMERASE_I_PROK, 1 hit
PS50880, TOPRIM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiO84649_CHLTR
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O84649
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1998
Last sequence update: November 1, 1998
Last modified: June 2, 2021
This is version 148 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiUnreviewed (UniProtKB/TrEMBL)

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteomeImported
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