UniProtKB - O82662 (SUCB_ARATH)
Protein
Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial
Gene
At2g20420
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation
Catalytic activityi
- EC:6.2.1.5UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
: tricarboxylic acid cycle Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (AXX17_At5g22800), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (AXX17_At2g15740), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (At2g20420), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, Uncharacterized protein (AN1_LOCUS8399), Succinate--CoA ligase [ADP-forming] subunit alpha-2, mitochondrial (At5g23250), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (AXX17_At5g08090), Succinate--CoA ligase [ADP-forming] subunit alpha, mitochondrial (MKD15.11), Succinate--CoA ligase [ADP-forming] subunit alpha-1, mitochondrial (At5g08300)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 74 | ATPUniRule annotation | 1 | |
Binding sitei | 141 | ATPUniRule annotation | 1 | |
Metal bindingi | 233 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 247 | MagnesiumUniRule annotation | 1 | |
Binding sitei | 298 | Substrate; shared with subunit alphaUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 81 – 83 | ATPUniRule annotation | 3 |
GO - Molecular functioni
- ATP binding Source: TAIR
- copper ion binding Source: TAIR
- magnesium ion binding Source: UniProtKB-UniRule
- succinate-CoA ligase (ADP-forming) activity Source: UniProtKB-UniRule
- succinate-CoA ligase (GDP-forming) activity Source: GO_Central
GO - Biological processi
- response to cadmium ion Source: TAIR
- succinyl-CoA metabolic process Source: GO_Central
- tricarboxylic acid cycle Source: GO_Central
Keywordsi
Molecular function | Ligase |
Biological process | Tricarboxylic acid cycle |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G20420-MONOMER |
UniPathwayi | UPA00223;UER00999 |
Names & Taxonomyi
Protein namesi | Recommended name: Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrialUniRule annotation (EC:6.2.1.5UniRule annotation)Alternative name(s): Succinyl-CoA synthetase beta chainUniRule annotation Short name: SCS-betaUniRule annotation |
Gene namesi | Ordered Locus Names:At2g20420 ORF Names:F11A3.3 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G20420 |
TAIRi | locus:2039037, AT2G20420 |
Subcellular locationi
Mitochondrion
- Mitochondrion UniRule annotation2 Publications
Mitochondrion
- mitochondrion Source: TAIR
Other locations
- succinate-CoA ligase complex Source: GO_Central
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 26 | Mitochondrion1 PublicationAdd BLAST | 26 | |
ChainiPRO_0000033362 | 27 – 421 | Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrialAdd BLAST | 395 |
Proteomic databases
PaxDbi | O82662 |
PRIDEi | O82662 |
ProteomicsDBi | 246381 |
PTM databases
MetOSitei | O82662 |
Expressioni
Gene expression databases
ExpressionAtlasi | O82662, baseline and differential |
Genevisiblei | O82662, AT |
Interactioni
Subunit structurei
Heterodimer of an alpha and a beta subunit.
UniRule annotationProtein-protein interaction databases
STRINGi | 3702.AT2G20420.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 278 | ATP-graspUniRule annotationAdd BLAST | 244 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 355 – 357 | Substrate binding; shared with subunit alphaUniRule annotation | 3 |
Sequence similaritiesi
Belongs to the succinate/malate CoA ligase beta subunit family.UniRule annotation
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG2799, Eukaryota |
HOGENOMi | CLU_037430_0_0_1 |
InParanoidi | O82662 |
OMAi | ITACDEV |
OrthoDBi | 973871at2759 |
PhylomeDBi | O82662 |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit 3.40.50.261, 1 hit |
HAMAPi | MF_00558, Succ_CoA_beta, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013650, ATP-grasp_succ-CoA_synth-type IPR013815, ATP_grasp_subdomain_1 IPR005811, CoA_ligase IPR017866, Succ-CoA_synthase_bsu_CS IPR005809, Succ_CoA_synthase_bsu IPR016102, Succinyl-CoA_synth-like |
PANTHERi | PTHR11815, PTHR11815, 1 hit |
Pfami | View protein in Pfam PF08442, ATP-grasp_2, 1 hit PF00549, Ligase_CoA, 1 hit |
PIRSFi | PIRSF001554, SucCS_beta, 1 hit |
SUPFAMi | SSF52210, SSF52210, 1 hit |
TIGRFAMsi | TIGR01016, sucCoAbeta, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS01217, SUCCINYL_COA_LIG_3, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O82662-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRGLVNKLVS RSLSISGKWQ NQQLRRLNIH EYQGAELMGK YGVNVPKGVA
60 70 80 90 100
ASSLEEVKKA IQDVFPNESE LVVKSQILAG GRGLGTFKSG LKGGVHIVKR
110 120 130 140 150
DEAEEIAGKM LGQVLVTKQT GPQGKVVSKV YLCEKLSLVN EMYFSIILDR
160 170 180 190 200
KSAGPLIIAC KKGGTSIEDL AEKFPDMIIK VPIDVFAGIT DEDAAKVVDG
210 220 230 240 250
LAPKAADRKD SIEQVKKLYE LFRKTDCTML EINPLAETST NQLVAADAKL
260 270 280 290 300
NFDDNAAFRQ KEVFAMRDPT QEDPREVAAA KVDLNYIGLD GEIGCMVNGA
310 320 330 340 350
GLAMATMDII KLHGGTPANF LDVGGNASEH QVVEAFKILT SDDKVKAILV
360 370 380 390 400
NIFGGIMKCD VIASGIVNAA KEVALKVPVV VRLEGTNVEQ GKRILKESGM
410 420
KLITADDLDD AAEKAVKALA H
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ001808 mRNA Translation: CAA05024.1 AC006569 Genomic DNA Translation: AAM15283.1 CP002685 Genomic DNA Translation: AEC07005.1 AY099707 mRNA Translation: AAM20558.1 AY128889 mRNA Translation: AAM91289.1 |
PIRi | T51809 |
RefSeqi | NP_179632.1, NM_127601.4 |
Genome annotation databases
EnsemblPlantsi | AT2G20420.1; AT2G20420.1; AT2G20420 |
GeneIDi | 816561 |
Gramenei | AT2G20420.1; AT2G20420.1; AT2G20420 |
KEGGi | ath:AT2G20420 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ001808 mRNA Translation: CAA05024.1 AC006569 Genomic DNA Translation: AAM15283.1 CP002685 Genomic DNA Translation: AEC07005.1 AY099707 mRNA Translation: AAM20558.1 AY128889 mRNA Translation: AAM91289.1 |
PIRi | T51809 |
RefSeqi | NP_179632.1, NM_127601.4 |
3D structure databases
SMRi | O82662 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G20420.1 |
PTM databases
MetOSitei | O82662 |
Proteomic databases
PaxDbi | O82662 |
PRIDEi | O82662 |
ProteomicsDBi | 246381 |
Genome annotation databases
EnsemblPlantsi | AT2G20420.1; AT2G20420.1; AT2G20420 |
GeneIDi | 816561 |
Gramenei | AT2G20420.1; AT2G20420.1; AT2G20420 |
KEGGi | ath:AT2G20420 |
Organism-specific databases
Araporti | AT2G20420 |
TAIRi | locus:2039037, AT2G20420 |
Phylogenomic databases
eggNOGi | KOG2799, Eukaryota |
HOGENOMi | CLU_037430_0_0_1 |
InParanoidi | O82662 |
OMAi | ITACDEV |
OrthoDBi | 973871at2759 |
PhylomeDBi | O82662 |
Enzyme and pathway databases
UniPathwayi | UPA00223;UER00999 |
BioCyci | ARA:AT2G20420-MONOMER |
Miscellaneous databases
PROi | PR:O82662 |
Gene expression databases
ExpressionAtlasi | O82662, baseline and differential |
Genevisiblei | O82662, AT |
Family and domain databases
Gene3Di | 3.30.1490.20, 1 hit 3.40.50.261, 1 hit |
HAMAPi | MF_00558, Succ_CoA_beta, 1 hit |
InterProi | View protein in InterPro IPR011761, ATP-grasp IPR013650, ATP-grasp_succ-CoA_synth-type IPR013815, ATP_grasp_subdomain_1 IPR005811, CoA_ligase IPR017866, Succ-CoA_synthase_bsu_CS IPR005809, Succ_CoA_synthase_bsu IPR016102, Succinyl-CoA_synth-like |
PANTHERi | PTHR11815, PTHR11815, 1 hit |
Pfami | View protein in Pfam PF08442, ATP-grasp_2, 1 hit PF00549, Ligase_CoA, 1 hit |
PIRSFi | PIRSF001554, SucCS_beta, 1 hit |
SUPFAMi | SSF52210, SSF52210, 1 hit |
TIGRFAMsi | TIGR01016, sucCoAbeta, 1 hit |
PROSITEi | View protein in PROSITE PS50975, ATP_GRASP, 1 hit PS01217, SUCCINYL_COA_LIG_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUCB_ARATH | |
Accessioni | O82662Primary (citable) accession number: O82662 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 27, 2002 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families