UniProtKB - O82647 (PDC1_ARATH)
Protein
Pyruvate decarboxylase 1
Gene
PDC1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
May play a role in ethanolic fermentation during anoxia.1 Publication
Catalytic activityi
- EC:4.1.1.1
Cofactori
Protein has several cofactor binding sites:- a metal cationNote: Binds 1 metal ion per subunit.
- thiamine diphosphateNote: Binds 1 thiamine pyrophosphate per subunit.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 69 | SubstrateBy similarity | 1 | |
Binding sitei | 156 | SubstrateBy similarity | 1 | |
Metal bindingi | 484 | MagnesiumBy similarity | 1 | |
Metal bindingi | 511 | MagnesiumBy similarity | 1 | |
Metal bindingi | 513 | Magnesium; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 517 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- carboxy-lyase activity Source: GO_Central
- magnesium ion binding Source: InterPro
- pyruvate decarboxylase activity Source: UniProtKB-EC
- thiamine pyrophosphate binding Source: InterPro
GO - Biological processi
- cellular response to hypoxia Source: TAIR
- response to anoxia Source: UniProtKB
Keywordsi
Molecular function | Decarboxylase, Lyase |
Biological process | Stress response |
Ligand | Magnesium, Metal-binding, Pyruvate, Thiamine pyrophosphate |
Enzyme and pathway databases
BioCyci | ARA:AT4G33070-MONOMER |
BRENDAi | 4.1.1.1, 399 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PDC1 Ordered Locus Names:At4g33070 ORF Names:F4I10.4 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G33070 |
TAIRi | locus:2123827, AT4G33070 |
Subcellular locationi
Cytosol
- cytosol Source: TAIR
Plasma Membrane
- plasma membrane Source: TAIR
Pathology & Biotechi
Disruption phenotypei
No visible phenotype under normal growth conditions, but mutant plant roots are more sensitive to anoxia.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422312 | 1 – 607 | Pyruvate decarboxylase 1Add BLAST | 607 |
Proteomic databases
PaxDbi | O82647 |
PRIDEi | O82647 |
ProteomicsDBi | 236289 |
Expressioni
Tissue specificityi
Highly expressed in seeds, and at lower levels in roots and siliques.1 Publication
Inductioni
By anoxia and abscisic acid (ABA).1 Publication
Gene expression databases
ExpressionAtlasi | O82647, baseline and differential |
Genevisiblei | O82647, AT |
Interactioni
Subunit structurei
Homotetramer.
CuratedProtein-protein interaction databases
BioGRIDi | 14729, 2 interactors |
STRINGi | 3702.AT4G33070.1 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 434 – 516 | Thiamine pyrophosphate bindingBy similarityAdd BLAST | 83 |
Sequence similaritiesi
Belongs to the TPP enzyme family.Curated
Phylogenomic databases
eggNOGi | KOG1184, Eukaryota |
HOGENOMi | CLU_013748_0_2_1 |
InParanoidi | O82647 |
OMAi | IFWGQVS |
OrthoDBi | 560466at2759 |
PhylomeDBi | O82647 |
Family and domain databases
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR029061, THDP-binding IPR012000, Thiamin_PyroP_enz_cen_dom IPR012001, Thiamin_PyroP_enz_TPP-bd_dom IPR000399, TPP-bd_CS IPR012110, TPP_enzyme IPR011766, TPP_enzyme-bd_C |
Pfami | View protein in Pfam PF02775, TPP_enzyme_C, 1 hit PF00205, TPP_enzyme_M, 1 hit PF02776, TPP_enzyme_N, 1 hit |
PIRSFi | PIRSF036565, Pyruvt_ip_decrb, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit SSF52518, SSF52518, 2 hits |
PROSITEi | View protein in PROSITE PS00187, TPP_ENZYMES, 1 hit |
i Sequence
Sequence statusi: Complete.
O82647-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDTKIGSIDD CKPTNGDVCS PTNGTVATIH NSVPSSAITI NYCDATLGRH
60 70 80 90 100
LARRLVQAGV TDVFSVPGDF NLTLLDHLMA EPDLNLIGCC NELNAGYAAD
110 120 130 140 150
GYARSRGVGA CVVTFTVGGL SVLNAIAGAY SENLPLICIV GGPNSNDYGT
160 170 180 190 200
NRILHHTIGL PDFSQELRCF QTVTCYQAVV NNLDDAHEQI DKAISTALKE
210 220 230 240 250
SKPVYISVSC NLAAIPHHTF SRDPVPFSLA PRLSNKMGLE AAVEATLEFL
260 270 280 290 300
NKAVKPVMVG GPKLRVAKAC DAFVELADAS GYALAMMPSA KGFVPEHHPH
310 320 330 340 350
FIGTYWGAVS TPFCSEIVES ADAYIFAGPI FNDYSSVGYS LLLKKEKAIV
360 370 380 390 400
VQPDRITVAN GPTFGCILMS DFFRELSKRV KRNETAYENY HRIFVPEGKP
410 420 430 440 450
LKCESREPLR VNTMFQHIQK MLSSETAVIA ETGDSWFNCQ KLKLPKGCGY
460 470 480 490 500
EFQMQYGSIG WSVGATLGYA QASPEKRVLA FIGDGSFQVT VQDISTMLRN
510 520 530 540 550
GQKTIIFLIN NGGYTIEVEI HDGPYNVIKN WNYTGLVDAI HNGEGNCWTA
560 570 580 590 600
KVRYEEELVE AITTATTEKK DCLCFIEVIL HKDDTSKELL EWGSRVSAAN
SRPPNPQ
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 220 | F → Y in AAB16854 (Ref. 1) Curated | 1 | |
Sequence conflicti | 256 | P → Q in AAB16854 (Ref. 1) Curated | 1 | |
Sequence conflicti | 429 | I → IA in AAB16854 (Ref. 1) Curated | 1 | |
Sequence conflicti | 447 – 449 | GCG → R in AAB16854 (Ref. 1) Curated | 3 | |
Sequence conflicti | 583 – 584 | DD → GE in AAB16854 (Ref. 1) Curated | 2 | |
Sequence conflicti | 594 – 603 | SRVSAANSRP → HASLLLTAVL in AAB16854 (Ref. 1) Curated | 10 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71121 Genomic DNA Translation: AAB16854.1 AL031804 Genomic DNA Translation: CAA21216.1 AL161582 Genomic DNA Translation: CAB80024.1 CP002687 Genomic DNA Translation: AEE86169.1 AY070036 mRNA Translation: AAL49793.1 AY122926 mRNA Translation: AAM67459.1 |
PIRi | T05315 |
RefSeqi | NP_195033.1, NM_119461.4 |
Genome annotation databases
EnsemblPlantsi | AT4G33070.1; AT4G33070.1; AT4G33070 |
GeneIDi | 829444 |
Gramenei | AT4G33070.1; AT4G33070.1; AT4G33070 |
KEGGi | ath:AT4G33070 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U71121 Genomic DNA Translation: AAB16854.1 AL031804 Genomic DNA Translation: CAA21216.1 AL161582 Genomic DNA Translation: CAB80024.1 CP002687 Genomic DNA Translation: AEE86169.1 AY070036 mRNA Translation: AAL49793.1 AY122926 mRNA Translation: AAM67459.1 |
PIRi | T05315 |
RefSeqi | NP_195033.1, NM_119461.4 |
3D structure databases
SMRi | O82647 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 14729, 2 interactors |
STRINGi | 3702.AT4G33070.1 |
Proteomic databases
PaxDbi | O82647 |
PRIDEi | O82647 |
ProteomicsDBi | 236289 |
Genome annotation databases
EnsemblPlantsi | AT4G33070.1; AT4G33070.1; AT4G33070 |
GeneIDi | 829444 |
Gramenei | AT4G33070.1; AT4G33070.1; AT4G33070 |
KEGGi | ath:AT4G33070 |
Organism-specific databases
Araporti | AT4G33070 |
TAIRi | locus:2123827, AT4G33070 |
Phylogenomic databases
eggNOGi | KOG1184, Eukaryota |
HOGENOMi | CLU_013748_0_2_1 |
InParanoidi | O82647 |
OMAi | IFWGQVS |
OrthoDBi | 560466at2759 |
PhylomeDBi | O82647 |
Enzyme and pathway databases
BioCyci | ARA:AT4G33070-MONOMER |
BRENDAi | 4.1.1.1, 399 |
Miscellaneous databases
PROi | PR:O82647 |
Gene expression databases
ExpressionAtlasi | O82647, baseline and differential |
Genevisiblei | O82647, AT |
Family and domain databases
InterProi | View protein in InterPro IPR029035, DHS-like_NAD/FAD-binding_dom IPR029061, THDP-binding IPR012000, Thiamin_PyroP_enz_cen_dom IPR012001, Thiamin_PyroP_enz_TPP-bd_dom IPR000399, TPP-bd_CS IPR012110, TPP_enzyme IPR011766, TPP_enzyme-bd_C |
Pfami | View protein in Pfam PF02775, TPP_enzyme_C, 1 hit PF00205, TPP_enzyme_M, 1 hit PF02776, TPP_enzyme_N, 1 hit |
PIRSFi | PIRSF036565, Pyruvt_ip_decrb, 1 hit |
SUPFAMi | SSF52467, SSF52467, 1 hit SSF52518, SSF52518, 2 hits |
PROSITEi | View protein in PROSITE PS00187, TPP_ENZYMES, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PDC1_ARATH | |
Accessioni | O82647Primary (citable) accession number: O82647 Secondary accession number(s): Q96535 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 29, 2013 |
Last sequence update: | November 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families