Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate dehydrogenase 1, peroxisomal

Gene

PMDH1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes a reversible NAD-dependent dehydrogenase reaction involved in central metabolism and redox homeostasis between organelle compartments (Probable). Peroxisomal NAD-dependent malate dehydrogenase involved in fatty acid beta-oxidation. Reoxidizes NADH from the beta-oxidation and provides NAD for the conversion of fatty acyl-CoA to acetyl-CoA. Does not participate directly in the glyoxylate cycle (PubMed:17376163, PubMed:19812894). Required for maintenance of photosynthetic rates under photorespiratory conditions, and carbon flow during photorespiration. Supplies NADH reductant to the peroxisomal hydroxypyruvate reductase (HPR), which reduces hydroxypyruvate into glycerate in the photorespiratory cycle (PubMed:18685043).1 Publication3 Publications

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei75NADBy similarity1
Binding sitei122SubstrateBy similarity1
Binding sitei128SubstrateBy similarity1
Binding sitei135NADBy similarity1
Binding sitei160SubstrateBy similarity1
Binding sitei194SubstrateBy similarity1
Active sitei218Proton acceptorBy similarity1
Binding sitei269NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi49 – 55NADBy similarity7
Nucleotide bindingi158 – 160NADBy similarity3

GO - Molecular functioni

GO - Biological processi

  • glyoxylate cycle Source: UniProtKB-KW
  • malate metabolic process Source: InterPro
  • regulation of fatty acid beta-oxidation Source: TAIR
  • regulation of photorespiration Source: TAIR
  • tricarboxylic acid cycle Source: UniProtKB-KW

Keywordsi

Molecular functionOxidoreductase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandNAD

Enzyme and pathway databases

BioCyciARA:AT2G22780-MONOMER
MetaCyc:AT2G22780-MONOMER
ReactomeiR-ATH-70263 Gluconeogenesis
R-ATH-71403 Citric acid cycle (TCA cycle)

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase 1, peroxisomalCurated (EC:1.1.1.37)
Alternative name(s):
Peroxisomal NAD-dependent malate dehydrogenase 1Curated
Short name:
pxNAD-MDH11 Publication
Peroxisomal malate dehydrogenase 11 Publication
Short name:
Peroxisomal MDH1Curated
Gene namesi
Name:PMDH11 Publication
Ordered Locus Names:At2g22780
ORF Names:T30L20.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G22780
TAIRilocus:2062240 AT2G22780

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but the double mutant plants pmdh1 and pmdh2 show seedling growth arrest 5 days after seed imbibition.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000186351 – 354Malate dehydrogenase 1, peroxisomalAdd BLAST354

Proteomic databases

PaxDbiO82399
PRIDEiO82399

Expressioni

Tissue specificityi

Expressed in rosette leaves at low levels.1 Publication

Gene expression databases

ExpressionAtlasiO82399 baseline and differential
GenevisibleiO82399 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi2161, 2 interactors
IntActiO82399, 1 interactor
STRINGi3702.AT2G22780.1

Structurei

3D structure databases

ProteinModelPortaliO82399
SMRiO82399
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 14Peroxisomal targeting signal PTS21 Publication9

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Phylogenomic databases

eggNOGiKOG1494 Eukaryota
COG0039 LUCA
HOGENOMiHOG000213792
InParanoidiO82399
KOiK00026
OMAiNDYERES
OrthoDBiEOG09360DTC
PhylomeDBiO82399

Family and domain databases

Gene3Di3.90.110.10, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR001252 Malate_DH_AS
IPR010097 Malate_DH_type1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01772 MDH_euk_gproteo, 1 hit
PROSITEiView protein in PROSITE
PS00068 MDH, 1 hit

Sequencei

Sequence statusi: Complete.

O82399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPNQRIARI SAHLNPPNLH NQIADGSGLN RVACRAKGGS PGFKVAILGA
60 70 80 90 100
AGGIGQPLAM LMKMNPLVSV LHLYDVANAP GVTADISHMD TSAVVRGFLG
110 120 130 140 150
QPQLEEALTG MDLVIIPAGV PRKPGMTRDD LFNINAGIVR TLSEAIAKCC
160 170 180 190 200
PKAIVNIISN PVNSTVPIAA EVFKKAGTFD PKKLMGVTML DVVRANTFVA
210 220 230 240 250
EVMSLDPREV EVPVVGGHAG VTILPLLSQV KPPCSFTQKE IEYLTDRIQN
260 270 280 290 300
GGTEVVEAKA GAGSATLSMA YAAVEFADAC LRGLRGDANI VECAYVASHV
310 320 330 340 350
TELPFFASKV RLGRCGIDEV YGLGPLNEYE RMGLEKAKKE LSVSIHKGVT

FAKK
Length:354
Mass (Da):37,466
Last modified:November 1, 1998 - v1
Checksum:i715CDC22B4448629
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005617 Genomic DNA Translation: AAC63589.1
CP002685 Genomic DNA Translation: AEC07353.1
AF428346 mRNA Translation: AAL16276.1
BT003009 mRNA Translation: AAO23574.1
PIRiG84616
RefSeqiNP_179863.1, NM_127843.5
UniGeneiAt.13158

Genome annotation databases

EnsemblPlantsiAT2G22780.1; AT2G22780.1; AT2G22780
GeneIDi816808
GrameneiAT2G22780.1; AT2G22780.1; AT2G22780
KEGGiath:AT2G22780

Similar proteinsi

Entry informationi

Entry nameiMDHX1_ARATH
AccessioniPrimary (citable) accession number: O82399
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 1, 1998
Last modified: April 25, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health