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UniProtKB - O81845 (PUMP1_ARATH)
Protein
Mitochondrial uncoupling protein 1
Gene
PUMP1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
PUMPS are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation. This leads to a decrease in the efficiency of oxidative phosphorylation and an increase in heat production. Is involved in protecting plant cells against oxidative stress damage and maintaining the redox balance of the mitochondrial electron transport chain to facilitate photosynthetic metabolism. May play a regulatory role during photorespiration.
6 PublicationsMiscellaneous
Over-expression of Arabidopsis UCP1 in tobacco plants increases tolerance to oxidative stress caused by exogenous hydrogen peroxide, or by drought and salt.1 Publication
GO - Molecular functioni
- amino acid transmembrane transporter activity Source: TAIR
- oxidative phosphorylation uncoupler activity Source: TAIR
GO - Biological processi
- amino acid transmembrane transport Source: TAIR
- mitochondrial transmembrane transport Source: GO_Central
- photorespiration Source: TAIR
Keywordsi
Biological process | Stress response, Transport |
Protein family/group databases
TCDBi | 2.A.29.3.3, the mitochondrial carrier (mc) family |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial uncoupling protein 1Short name: AtPUMP1 |
Gene namesi | Name:PUMP1 Synonyms:UCP1 Ordered Locus Names:At3g54110 ORF Names:F24B22.70 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G54110 |
TAIRi | locus:2080300, AT3G54110 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
Cytosol
- cytosol Source: TAIR
Mitochondrion
- mitochondrial inner membrane Source: TAIR
- mitochondrion Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
- plasmodesma Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 15 – 35 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Transmembranei | 71 – 91 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Transmembranei | 118 – 138 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Transmembranei | 177 – 197 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Transmembranei | 218 – 238 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Transmembranei | 269 – 289 | Helical; Name=6Sequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePathology & Biotechi
Disruption phenotypei
Slight reduction of shoot mass.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 28 | C → A: Reduces proton transport activity 3-fold. 1 Publication | 1 | |
Mutagenesisi | 83 | H → L: Reduces proton transport activity 3-fold. 1 Publication | 1 | |
Mutagenesisi | 147 | K → H: Reduces proton transport activity 3-fold. 1 Publication | 1 | |
Mutagenesisi | 155 | R → L: Slightly reduces proton transport activity. 1 Publication | 1 | |
Mutagenesisi | 269 | Y → F: Reduces proton transport activity 3-fold. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000420255 | 1 – 306 | Mitochondrial uncoupling protein 1Add BLAST | 306 |
Proteomic databases
PaxDbi | O81845 |
ProteomicsDBi | 226359 |
Expressioni
Tissue specificityi
Widely expressed.2 Publications
Inductioni
By cold stress, high light and chloramphenicol.3 Publications
Gene expression databases
ExpressionAtlasi | O81845, baseline and differential |
Genevisiblei | O81845, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 9895, 1 interactor |
STRINGi | 3702.AT3G54110.1 |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 9 – 102 | Solcar 1Add BLAST | 94 | |
Repeati | 112 – 203 | Solcar 2Add BLAST | 92 | |
Repeati | 212 – 296 | Solcar 3Add BLAST | 85 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0753, Eukaryota |
HOGENOMi | CLU_015166_14_2_1 |
InParanoidi | O81845 |
OMAi | RACLMTA |
OrthoDBi | 984118at2759 |
PhylomeDBi | O81845 |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
i Sequence
Sequence statusi: Complete.
O81845-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVAAGKSDLS LPKTFACSAF AACVGEVCTI PLDTAKVRLQ LQKSALAGDV
60 70 80 90 100
TLPKYRGLLG TVGTIAREEG LRSLWKGVVP GLHRQCLFGG LRIGMYEPVK
110 120 130 140 150
NLYVGKDFVG DVPLSKKILA GLTTGALGIM VANPTDLVKV RLQAEGKLAA
160 170 180 190 200
GAPRRYSGAL NAYSTIVRQE GVRALWTGLG PNVARNAIIN AAELASYDQV
210 220 230 240 250
KETILKIPGF TDNVVTHILS GLGAGFFAVC IGSPVDVVKS RMMGDSGAYK
260 270 280 290 300
GTIDCFVKTL KSDGPMAFYK GFIPNFGRLG SWNVIMFLTL EQAKKYVREL
DASKRN
Sequence cautioni
The sequence BAD95028 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 178 | G → V in CAA11757 (PubMed:9662458).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223983 mRNA Translation: CAA11757.1 AJ001264 mRNA Translation: CAA04638.1 Y18291 Genomic DNA Translation: CAA77109.1 AF146226 mRNA Translation: AAF66705.1 AL132957 Genomic DNA Translation: CAB70985.1 CP002686 Genomic DNA Translation: AEE79189.1 AY056272 mRNA Translation: AAL07121.1 AY091185 mRNA Translation: AAM14124.1 AK221112 mRNA Translation: BAD95028.1 Different initiation. |
PIRi | T47570 T52024 |
RefSeqi | NP_190979.1, NM_115271.5 |
Genome annotation databases
EnsemblPlantsi | AT3G54110.1; AT3G54110.1; AT3G54110 |
GeneIDi | 824578 |
Gramenei | AT3G54110.1; AT3G54110.1; AT3G54110 |
KEGGi | ath:AT3G54110 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223983 mRNA Translation: CAA11757.1 AJ001264 mRNA Translation: CAA04638.1 Y18291 Genomic DNA Translation: CAA77109.1 AF146226 mRNA Translation: AAF66705.1 AL132957 Genomic DNA Translation: CAB70985.1 CP002686 Genomic DNA Translation: AEE79189.1 AY056272 mRNA Translation: AAL07121.1 AY091185 mRNA Translation: AAM14124.1 AK221112 mRNA Translation: BAD95028.1 Different initiation. |
PIRi | T47570 T52024 |
RefSeqi | NP_190979.1, NM_115271.5 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 9895, 1 interactor |
STRINGi | 3702.AT3G54110.1 |
Protein family/group databases
TCDBi | 2.A.29.3.3, the mitochondrial carrier (mc) family |
Proteomic databases
PaxDbi | O81845 |
ProteomicsDBi | 226359 |
Genome annotation databases
EnsemblPlantsi | AT3G54110.1; AT3G54110.1; AT3G54110 |
GeneIDi | 824578 |
Gramenei | AT3G54110.1; AT3G54110.1; AT3G54110 |
KEGGi | ath:AT3G54110 |
Organism-specific databases
Araporti | AT3G54110 |
TAIRi | locus:2080300, AT3G54110 |
Phylogenomic databases
eggNOGi | KOG0753, Eukaryota |
HOGENOMi | CLU_015166_14_2_1 |
InParanoidi | O81845 |
OMAi | RACLMTA |
OrthoDBi | 984118at2759 |
PhylomeDBi | O81845 |
Miscellaneous databases
PROi | PR:O81845 |
Gene expression databases
ExpressionAtlasi | O81845, baseline and differential |
Genevisiblei | O81845, AT |
Family and domain databases
Gene3Di | 1.50.40.10, 1 hit |
InterProi | View protein in InterPro IPR002030, Mit_uncoupling_UCP-like IPR018108, Mitochondrial_sb/sol_carrier IPR023395, Mt_carrier_dom_sf |
Pfami | View protein in Pfam PF00153, Mito_carr, 3 hits |
PRINTSi | PR00784, MTUNCOUPLING |
SUPFAMi | SSF103506, SSF103506, 1 hit |
PROSITEi | View protein in PROSITE PS50920, SOLCAR, 3 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | PUMP1_ARATH | |
Accessioni | O81845Primary (citable) accession number: O81845 Secondary accession number(s): O65623, Q56Z57 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2012 |
Last sequence update: | November 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 144 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families