UniProtKB - O81643 (VILI1_ARATH)
Protein
Villin-1
Gene
VLN1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Binds actin and actin filament bundles in a Ca2+/calmodulin-insensitive manner, but is unable to sever, cap, and nucleate actin filament formation in vitro. Does not protect individual filaments from severing by VLN3 (AC O81645).3 Publications
GO - Molecular functioni
- actin filament binding Source: InterPro
GO - Biological processi
- actin filament capping Source: UniProtKB-KW
- actin filament severing Source: InterPro
- cytoskeleton organization Source: InterPro
Keywordsi
Molecular function | Actin capping, Actin-binding |
Ligand | Calcium |
Names & Taxonomyi
Protein namesi | Recommended name: Villin-11 Publication |
Gene namesi | Name:VLN11 Publication Ordered Locus Names:At2g29890Imported ORF Names:F6K5.2Imported, T27A16.1 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G29890 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000218732 | 1 – 909 | Villin-1Add BLAST | 909 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 780 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O81643 |
ProteomicsDBi | 242664 [O81643-1] |
PTM databases
iPTMneti | O81643 |
Expressioni
Tissue specificityi
Expressed in all tissues examined. Mainly detected in the vascular tissue and the pericycle of roots and in the vasculature of leaves. Not expressed in the root cap.2 Publications
Gene expression databases
ExpressionAtlasi | O81643, baseline and differential |
Genevisiblei | O81643, AT |
Interactioni
GO - Molecular functioni
- actin filament binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 2889, 1 interactor |
STRINGi | 3702.AT2G29890.3 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 29 – 79 | Gelsolin-like 1Sequence analysisAdd BLAST | 51 | |
Repeati | 149 – 189 | Gelsolin-like 2Sequence analysisAdd BLAST | 41 | |
Repeati | 262 – 305 | Gelsolin-like 3Sequence analysisAdd BLAST | 44 | |
Repeati | 391 – 448 | Gelsolin-like 4Sequence analysisAdd BLAST | 58 | |
Repeati | 529 – 569 | Gelsolin-like 5Sequence analysisAdd BLAST | 41 | |
Repeati | 631 – 672 | Gelsolin-like 6Sequence analysisAdd BLAST | 42 | |
Domaini | 844 – 909 | HPPROSITE-ProRule annotationAdd BLAST | 66 |
Sequence similaritiesi
Belongs to the villin/gelsolin family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG0443, Eukaryota |
InParanoidi | O81643 |
OMAi | RGTSANN |
PhylomeDBi | O81643 |
Family and domain databases
Gene3Di | 1.10.950.10, 1 hit 3.40.20.10, 6 hits |
InterProi | View protein in InterPro IPR029006, ADF-H/Gelsolin-like_dom_sf IPR007123, Gelsolin-like_dom IPR030010, Villin-1-like_plant IPR007122, Villin/Gelsolin IPR003128, Villin_headpiece IPR036886, Villin_headpiece_dom_sf |
PANTHERi | PTHR11977, PTHR11977, 1 hit PTHR11977:SF25, PTHR11977:SF25, 1 hit |
Pfami | View protein in Pfam PF00626, Gelsolin, 4 hits PF02209, VHP, 1 hit |
PRINTSi | PR00597, GELSOLIN |
SMARTi | View protein in SMART SM00262, GEL, 6 hits SM00153, VHP, 1 hit |
SUPFAMi | SSF47050, SSF47050, 1 hit |
PROSITEi | View protein in PROSITE PS51089, HP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O81643-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSRLSKDIDS AFQGVGTKSG LEIWCVYNKQ LISIPKSSFG KFHSGNAYLV
60 70 80 90 100
LRTFLRKIES PQYDIHYWLG IDANEVDSIL ASDKALDLDA ALGCCTVQYR
110 120 130 140 150
EVQGQETEKF LSYFKPCIIP VEGKYSPKTG IAGETYQVTL LRCKGDHVVR
160 170 180 190 200
VKEVPFLRSS LNHDDVFILD TASKVFLFAG CNSSTQEKAK AMEVVEYIKD
210 220 230 240 250
NKHDGRCEVA TIEDGKFSGD SDAGEFWSFF GGYAPIPKLS SSTTQEQTQT
260 270 280 290 300
PCAELFWIDT KGNLHPTGTS SLDKDMLEKN KCYMLDCHSE VFVWMGRNTS
310 320 330 340 350
LTERKTSISS SEEFLRKEGR STTTSLVLLT EGLENARFRS FFNKWPQTVE
360 370 380 390 400
SSLYNEGREK VAALFKQKGY DVEELPDEED DPLYTNCRDN LKVWRVDGDD
410 420 430 440 450
VSLLSIPDQT KLFTGDCYLV QYKYTYKERT EHLLYVWIGC ESIQQDRADA
460 470 480 490 500
ITNASAIVGT TKGESVLCHI YQGNEPSRFF PMFQSLVVFK GGLSRRYKVL
510 520 530 540 550
LAEKEKIGEE YNENKASLFR VVGTSPRNMQ AIQVNLVATS LNSSYSYILQ
560 570 580 590 600
YGASAFTWIG KLSSDSDHEV LDRMLYFLDT SCQPIYIREG NETDTFWNLL
610 620 630 640 650
GGKSEYPKEK EMRKQIEEPH LFTCSCSSDV LKVKEIYNFV QDDLTTEDVF
660 670 680 690 700
LLDCQSEVYV WIGSNSNIKS KEEALTLGLK FLEMDILEEG LTMRTPVYVV
710 720 730 740 750
TEGHEPPFFT RFFEWVPEKA NMHGNSFERK LASLKGKKTS TKRSSGSQYR
760 770 780 790 800
SQSKDNASRD LQSRSVSSNG SERGVSPCSS EKLLSLSSAE DMTNSSNSTP
810 820 830 840 850
VVKKLFSESL LVDPNDGVAR QESSSKSDIS KQKPRVGINS DLSSLESLAY
860 870 880 890 900
SYEQLRVDSQ KPVTDIDATR REAYLTEKEF EERFGMAKSE FYALPKWKQN
KLKISLHLF
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF4ILN8 | F4ILN8_ARATH | Villin-like 1 | VLN1 ATVLN1, villin 1, villin-like 1, At2g29890, F6K5.2 | 933 | Annotation score: | ||
Q2V445 | Q2V445_ARATH | Villin-like 1 | VLN1 ATVLN1, villin 1, villin-like 1, At2g29890, F6K5.2 | 911 | Annotation score: | ||
A0A1P8AZB7 | A0A1P8AZB7_ARATH | Villin-like 1 | VLN1 ATVLN1, villin 1, villin-like 1, At2g29890, F6K5.2 | 892 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | I → F in AAC31605 (PubMed:10631247).Curated | 1 | |
Sequence conflicti | 347 – 353 | QTVESSL → SDRWSLAF in AAC31605 (PubMed:10631247).Curated | 7 | |
Sequence conflicti | 585 | I → T in AAC31605 (PubMed:10631247).Curated | 1 | |
Sequence conflicti | 811 | L → V in AAC31605 (PubMed:10631247).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF081201 mRNA Translation: AAC31605.1 AC007113 Genomic DNA Translation: AAD23629.2 CP002685 Genomic DNA Translation: AEC08315.1 CP002685 Genomic DNA Translation: ANM61989.1 |
PIRi | H84701 T50671 |
RefSeqi | NP_001324173.1, NM_001336228.1 [O81643-1] NP_029567.1, NM_128543.4 [O81643-1] |
Genome annotation databases
EnsemblPlantsi | AT2G29890.1; AT2G29890.1; AT2G29890 [O81643-1] AT2G29890.5; AT2G29890.5; AT2G29890 [O81643-1] |
GeneIDi | 817539 |
Gramenei | AT2G29890.1; AT2G29890.1; AT2G29890 [O81643-1] AT2G29890.5; AT2G29890.5; AT2G29890 [O81643-1] |
KEGGi | ath:AT2G29890 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF081201 mRNA Translation: AAC31605.1 AC007113 Genomic DNA Translation: AAD23629.2 CP002685 Genomic DNA Translation: AEC08315.1 CP002685 Genomic DNA Translation: ANM61989.1 |
PIRi | H84701 T50671 |
RefSeqi | NP_001324173.1, NM_001336228.1 [O81643-1] NP_029567.1, NM_128543.4 [O81643-1] |
3D structure databases
SMRi | O81643 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 2889, 1 interactor |
STRINGi | 3702.AT2G29890.3 |
PTM databases
iPTMneti | O81643 |
Proteomic databases
PaxDbi | O81643 |
ProteomicsDBi | 242664 [O81643-1] |
Genome annotation databases
EnsemblPlantsi | AT2G29890.1; AT2G29890.1; AT2G29890 [O81643-1] AT2G29890.5; AT2G29890.5; AT2G29890 [O81643-1] |
GeneIDi | 817539 |
Gramenei | AT2G29890.1; AT2G29890.1; AT2G29890 [O81643-1] AT2G29890.5; AT2G29890.5; AT2G29890 [O81643-1] |
KEGGi | ath:AT2G29890 |
Organism-specific databases
Araporti | AT2G29890 |
Phylogenomic databases
eggNOGi | KOG0443, Eukaryota |
InParanoidi | O81643 |
OMAi | RGTSANN |
PhylomeDBi | O81643 |
Miscellaneous databases
PROi | PR:O81643 |
Gene expression databases
ExpressionAtlasi | O81643, baseline and differential |
Genevisiblei | O81643, AT |
Family and domain databases
Gene3Di | 1.10.950.10, 1 hit 3.40.20.10, 6 hits |
InterProi | View protein in InterPro IPR029006, ADF-H/Gelsolin-like_dom_sf IPR007123, Gelsolin-like_dom IPR030010, Villin-1-like_plant IPR007122, Villin/Gelsolin IPR003128, Villin_headpiece IPR036886, Villin_headpiece_dom_sf |
PANTHERi | PTHR11977, PTHR11977, 1 hit PTHR11977:SF25, PTHR11977:SF25, 1 hit |
Pfami | View protein in Pfam PF00626, Gelsolin, 4 hits PF02209, VHP, 1 hit |
PRINTSi | PR00597, GELSOLIN |
SMARTi | View protein in SMART SM00262, GEL, 6 hits SM00153, VHP, 1 hit |
SUPFAMi | SSF47050, SSF47050, 1 hit |
PROSITEi | View protein in PROSITE PS51089, HP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | VILI1_ARATH | |
Accessioni | O81643Primary (citable) accession number: O81643 Secondary accession number(s): O82367, Q9SIK8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 15, 2002 |
Last sequence update: | May 15, 2007 | |
Last modified: | December 2, 2020 | |
This is version 140 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families