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UniProtKB - O81210 (RBOHC_ARATH)
Protein
Respiratory burst oxidase homolog protein C
Gene
RBOHC
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Calcium-dependent NADPH oxidase that generates superoxide. Required for H2O2 production in response to K+ deficiency and for the generation of reactive oxygen species (ROS) that regulate cell expansion through the activation of Ca2+ channels.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 239 | CalciumBy similarity | 1 | |
Metal bindingi | 241 | CalciumBy similarity | 1 | |
Metal bindingi | 243 | CalciumBy similarity | 1 | |
Metal bindingi | 245 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 250 | CalciumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 239 – 250 | PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- NAD(P)H oxidase H2O2-forming activity Source: TAIR
- peroxidase activity Source: UniProtKB-KW
GO - Biological processi
- aerobic respiration Source: TAIR
- response to ATP Source: TAIR
- root epidermal cell differentiation Source: TAIR
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Ligand | Calcium, FAD, Flavoprotein, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | ARA:AT5G51060-MONOMER |
Protein family/group databases
PeroxiBasei | 3284, AtRboh03 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RBOHC Synonyms:RHD2 Ordered Locus Names:At5g51060 ORF Names:K3K7.25 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G51060 |
TAIRi | locus:2157348, AT5G51060 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- integral component of plasma membrane Source: TAIR
- plasma membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 351 | CytoplasmicSequence analysisAdd BLAST | 351 | |
Transmembranei | 352 – 372 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 373 – 436 | ExtracellularSequence analysisAdd BLAST | 64 | |
Transmembranei | 437 – 457 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 458 – 492 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 493 – 513 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 514 – 536 | ExtracellularSequence analysisAdd BLAST | 23 | |
Transmembranei | 537 – 557 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 558 – 565 | CytoplasmicSequence analysis | 8 | |
Transmembranei | 566 – 583 | Helical; Name=5By similarityAdd BLAST | 18 | |
Topological domaini | 584 – 713 | ExtracellularSequence analysisAdd BLAST | 130 | |
Transmembranei | 714 – 734 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 735 – 905 | CytoplasmicSequence analysisAdd BLAST | 171 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
Plants have short root hairs and stunted roots.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000313755 | 1 – 905 | Respiratory burst oxidase homolog protein CAdd BLAST | 905 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 25 | PhosphoserineBy similarity | 1 | |
Modified residuei | 318 | PhosphoserineBy similarity | 1 | |
Modified residuei | 322 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O81210 |
ProteomicsDBi | 225973 |
PTM databases
iPTMneti | O81210 |
Expressioni
Tissue specificityi
In roots, expressed in the epidermis in the proximal regions of the meristem, in the elongation zone, in the differentiation zone and in elongating root hairs.2 Publications
Inductioni
By potassium starvation.1 Publication
Gene expression databases
ExpressionAtlasi | O81210, baseline and differential |
Genevisiblei | O81210, AT |
Interactioni
Subunit structurei
Monomer and homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 20424, 15 interactors |
IntActi | O81210, 15 interactors |
STRINGi | 3702.AT5G51060.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 226 – 261 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 270 – 305 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 390 – 549 | Ferric oxidoreductaseAdd BLAST | 160 | |
Domaini | 587 – 711 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 125 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 49 | DisorderedSequence analysisAdd BLAST | 49 | |
Regioni | 168 – 178 | EF-hand-like 1By similarityAdd BLAST | 11 | |
Regioni | 203 – 214 | EF-hand-like 2By similarityAdd BLAST | 12 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
HOGENOMi | CLU_005646_6_0_1 |
InParanoidi | O81210 |
OMAi | ARCSAYI |
OrthoDBi | 936110at2759 |
PhylomeDBi | O81210 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR013623, NADPH_Ox IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit PF08414, NADPH_Ox, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
O81210-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSRVSFEVSG GYHSDAEAGN SGPMSGGQLP PIYKKPGNSR FTAENSQRTR
60 70 80 90 100
TAPYVDLTVD VQDDTVSVHS LKMEGGSSVE ESPELTLLKR NRLEKKTTVV
110 120 130 140 150
KRLASVSHEL KRLTSVSGGI GGRKPPRPAK LDRTKSAASQ ALKGLKFISK
160 170 180 190 200
TDGGAGWSAV EKRFNQITAT TGGLLLRTKF GECIGMTSKD FALELFDALA
210 220 230 240 250
RRRNITGEVI DGDQLKEFWE QINDQSFDSR LKTFFDMVDK DADGRLTEDE
260 270 280 290 300
VREIISLSAS ANNLSTIQKR ADEYAALIME ELDPDNIGYI MLESLETLLL
310 320 330 340 350
QAATQSVITS TGERKNLSHM MSQRLKPTFN RNPLKRWYRG LRFFLLDNWQ
360 370 380 390 400
RCWVIVLWFI VMAILFTYKY IQYRRSPVYP VMGDCVCMAK GAAETVKLNM
410 420 430 440 450
ALILLPVCRN TITWLRNKTR LGRVVPFDDN LNFHKVIAVG IIVGVTMHAG
460 470 480 490 500
AHLACDFPRL LHATPEAYRP LRQFFGDEQP KSYWHFVNSV EGITGLVMVL
510 520 530 540 550
LMAIAFTLAT PWFRRGKLNY LPGPLKKLAS FNAFWYTHHL FVIVYILLVA
560 570 580 590 600
HGYYLYLTRD WHNKTTWMYL VVPVVLYACE RLIRAFRSSI KAVTIRKVAV
610 620 630 640 650
YPGNVLAIHL SRPQNFKYKS GQYMFVNCAA VSPFEWHPFS ITSAPQDDYL
660 670 680 690 700
SVHIRVLGDW TRALKGVFSE VCKPPPAGVS GLLRADMLHG ANNPDFPKVL
710 720 730 740 750
IDGPYGAPAQ DYKKYEVVLL VGLGIGATPM ISIVKDIVNN IKAKEQAQLN
760 770 780 790 800
RMENGTSEPQ RSKKESFRTR RAYFYWVTRE QGSFDWFKNI MNEVAERDAN
810 820 830 840 850
RVIEMHNYCT SVYEEGDARS ALIHMLQSLN HAKNGVDIVS GTRVMSHFAK
860 870 880 890 900
PNWRNVYKRI AMDHPNTKVG VFYCGAPALT KELRHLALDF THKTSTRFSF
HKENF
Sequence cautioni
The sequence AAC39477 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 676 | P → L in BAC41837 (PubMed:11910074).Curated | 1 | |
Sequence conflicti | 870 | G → R in AAS15724 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF055355 Genomic DNA Translation: AAC39477.1 Sequence problems. AY452508 Genomic DNA Translation: AAS15724.1 AB017063 Genomic DNA Translation: BAB08752.1 CP002688 Genomic DNA Translation: AED96028.1 AK117159 mRNA Translation: BAC41837.1 |
RefSeqi | NP_199919.1, NM_124485.3 |
Genome annotation databases
EnsemblPlantsi | AT5G51060.1; AT5G51060.1; AT5G51060 |
GeneIDi | 835179 |
Gramenei | AT5G51060.1; AT5G51060.1; AT5G51060 |
KEGGi | ath:AT5G51060 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF055355 Genomic DNA Translation: AAC39477.1 Sequence problems. AY452508 Genomic DNA Translation: AAS15724.1 AB017063 Genomic DNA Translation: BAB08752.1 CP002688 Genomic DNA Translation: AED96028.1 AK117159 mRNA Translation: BAC41837.1 |
RefSeqi | NP_199919.1, NM_124485.3 |
3D structure databases
SMRi | O81210 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 20424, 15 interactors |
IntActi | O81210, 15 interactors |
STRINGi | 3702.AT5G51060.1 |
Protein family/group databases
PeroxiBasei | 3284, AtRboh03 |
PTM databases
iPTMneti | O81210 |
Proteomic databases
PaxDbi | O81210 |
ProteomicsDBi | 225973 |
Genome annotation databases
EnsemblPlantsi | AT5G51060.1; AT5G51060.1; AT5G51060 |
GeneIDi | 835179 |
Gramenei | AT5G51060.1; AT5G51060.1; AT5G51060 |
KEGGi | ath:AT5G51060 |
Organism-specific databases
Araporti | AT5G51060 |
TAIRi | locus:2157348, AT5G51060 |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
HOGENOMi | CLU_005646_6_0_1 |
InParanoidi | O81210 |
OMAi | ARCSAYI |
OrthoDBi | 936110at2759 |
PhylomeDBi | O81210 |
Enzyme and pathway databases
BioCyci | ARA:AT5G51060-MONOMER |
Miscellaneous databases
PROi | PR:O81210 |
Gene expression databases
ExpressionAtlasi | O81210, baseline and differential |
Genevisiblei | O81210, AT |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR013623, NADPH_Ox IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit PF08414, NADPH_Ox, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 2 hits PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBOHC_ARATH | |
Accessioni | O81210Primary (citable) accession number: O81210 Secondary accession number(s): Q5VI40, Q8GZ82, Q9FI42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | February 23, 2022 | |
This is version 138 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families