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UniProtKB - O81209 (RBOHA_ARATH)
Protein
Respiratory burst oxidase homolog protein A
Gene
RBOHA
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Calcium-dependent NADPH oxidase that generates superoxide.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 234 | CalciumBy similarity | 1 | |
Metal bindingi | 236 | CalciumBy similarity | 1 | |
Metal bindingi | 238 | CalciumBy similarity | 1 | |
Metal bindingi | 240 | Calcium; via carbonyl oxygenBy similarity | 1 | |
Metal bindingi | 245 | CalciumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Calcium bindingi | 234 – 245 | PROSITE-ProRule annotationAdd BLAST | 12 |
GO - Molecular functioni
- calcium ion binding Source: TAIR
- NAD(P)H oxidase H2O2-forming activity Source: GO_Central
- peroxidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase, Peroxidase |
Ligand | Calcium, FAD, Flavoprotein, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | ARA:AT5G07390-MONOMER |
Protein family/group databases
PeroxiBasei | 3282, AtRboh01 |
TCDBi | 5.B.1.1.8, the phagocyte (gp91(phox)) nadph oxidase family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:RBOHA Ordered Locus Names:At5g07390 ORF Names:T2I1.100 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT5G07390 |
TAIRi | locus:2183309, AT5G07390 |
Subcellular locationi
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Plasma Membrane
- intrinsic component of plasma membrane Source: TAIR
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 344 | CytoplasmicSequence analysisAdd BLAST | 344 | |
Transmembranei | 345 – 365 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 366 – 380 | ExtracellularSequence analysisAdd BLAST | 15 | |
Transmembranei | 381 – 401 | Helical; Name=2By similarityAdd BLAST | 21 | |
Topological domaini | 402 – 428 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 429 – 449 | Helical; Name=3By similarityAdd BLAST | 21 | |
Topological domaini | 450 – 484 | ExtracellularSequence analysisAdd BLAST | 35 | |
Transmembranei | 485 – 505 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 506 – 529 | CytoplasmicSequence analysisAdd BLAST | 24 | |
Transmembranei | 530 – 550 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 551 – 709 | ExtracellularSequence analysisAdd BLAST | 159 | |
Transmembranei | 710 – 730 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 731 – 902 | CytoplasmicSequence analysisAdd BLAST | 172 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000313753 | 1 – 902 | Respiratory burst oxidase homolog protein AAdd BLAST | 902 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 311 | PhosphoserineBy similarity | 1 | |
Modified residuei | 315 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | O81209 |
ProteomicsDBi | 225940 [O81209-1] |
Expressioni
Gene expression databases
ExpressionAtlasi | O81209, baseline and differential |
Genevisiblei | O81209, AT |
Interactioni
Subunit structurei
Monomer and homodimer.
By similarityProtein-protein interaction databases
STRINGi | 3702.AT5G07390.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 221 – 256 | EF-hand 1PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 265 – 300 | EF-hand 2PROSITE-ProRule annotationAdd BLAST | 36 | |
Domaini | 383 – 540 | Ferric oxidoreductaseAdd BLAST | 158 | |
Domaini | 575 – 703 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 63 – 87 | DisorderedSequence analysisAdd BLAST | 25 | |
Regioni | 107 – 130 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 163 – 173 | EF-hand-like 1By similarityAdd BLAST | 11 | |
Regioni | 198 – 209 | EF-hand-like 2By similarityAdd BLAST | 12 | |
Regioni | 738 – 760 | DisorderedSequence analysisAdd BLAST | 23 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 70 – 87 | Polar residuesSequence analysisAdd BLAST | 18 |
Sequence similaritiesi
Keywords - Domaini
Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0039, Eukaryota |
InParanoidi | O81209 |
OMAi | SEVCNPR |
OrthoDBi | 936110at2759 |
PhylomeDBi | O81209 |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR013623, NADPH_Ox IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit PF08414, NADPH_Ox, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS51384, FAD_FR, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O81209-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MMNRSEMQKL GFEHVRYYTE SPYNRGESSA NVATTSNYYG EDEPYVEITL
60 70 80 90 100
DIHDDSVSVY GLKSPNHRGA GSNYEDQSLL RQGRSGRSNS VLKRLASSVS
110 120 130 140 150
TGITRVASSV SSSSARKPPR PQLAKLRRSK SRAELALKGL KFITKTDGVT
160 170 180 190 200
GWPEVEKRFY VMTMTTNGLL HRSRFGECIG MKSTEFALAL FDALARRENV
210 220 230 240 250
SGDSININEL KEFWKQITDQ DFDSRLRTFF AMVDKDSDGR LNEAEVREII
260 270 280 290 300
TLSASANELD NIRRQADEYA ALIMEELDPY HYGYIMIENL EILLLQAPMQ
310 320 330 340 350
DVRDGEGKKL SKMLSQNLMV PQSRNLGARF CRGMKYFLFD NWKRVWVMAL
360 370 380 390 400
WIGAMAGLFT WKFMEYRKRS AYEVMGVCVC IAKGAAETLK LNMAMILLPV
410 420 430 440 450
CRNTITWLRT KTKLSAIVPF DDSLNFHKVI AIGISVGVGI HATSHLACDF
460 470 480 490 500
PRLIAADEDQ YEPMEKYFGP QTKRYLDFVQ SVEGVTGIGM VVLMTIAFTL
510 520 530 540 550
ATTWFRRNKL NLPGPLKKIT GFNAFWYSHH LFVIVYSLLV VHGFYVYLII
560 570 580 590 600
EPWYKKTTWM YLMVPVVLYL CERLIRAFRS SVEAVSVLKV AVLPGNVLSL
610 620 630 640 650
HLSRPSNFRY KSGQYMYLNC SAVSTLEWHP FSITSAPGDD YLSVHIRVLG
660 670 680 690 700
DWTKQLRSLF SEVCKPRPPD EHRLNRADSK HWDYIPDFPR ILIDGPYGAP
710 720 730 740 750
AQDYKKFEVV LLVGLGIGAT PMISIVSDII NNLKGVEEGS NRRQSPIHNM
760 770 780 790 800
VTPPVSPSRK SETFRTKRAY FYWVTREQGS FDWFKNVMDE VTETDRKNVI
810 820 830 840 850
ELHNYCTSVY EEGDARSALI TMLQSLNHAK HGVDVVSGTR VMSHFARPNW
860 870 880 890 900
RSVFKRIAVN HPKTRVGVFY CGAAGLVKEL RHLSLDFSHK TSTKFIFHKE
NF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4K6P2 | F4K6P2_ARATH | Respiratory burst oxidase homolog A | RBOHA ATRBOHA, At5g07390, T2I1.100, T2I1_100 | 840 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 166 | T → N in AAC39475 (PubMed:9628030).Curated | 1 | |
Sequence conflicti | 207 | I → M in AAC39475 (PubMed:9628030).Curated | 1 | |
Sequence conflicti | 307 | G → S in AAC39475 (PubMed:9628030).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF055353 Genomic DNA Translation: AAC39475.1 AL163912 Genomic DNA Translation: CAB87928.1 CP002688 Genomic DNA Translation: AED91151.1 BT003857 mRNA Translation: AAO41907.1 |
PIRi | T49878 |
RefSeqi | NP_196356.1, NM_120821.2 [O81209-1] |
Genome annotation databases
EnsemblPlantsi | AT5G07390.1; AT5G07390.1; AT5G07390 [O81209-1] |
GeneIDi | 830630 |
Gramenei | AT5G07390.1; AT5G07390.1; AT5G07390 [O81209-1] |
KEGGi | ath:AT5G07390 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF055353 Genomic DNA Translation: AAC39475.1 AL163912 Genomic DNA Translation: CAB87928.1 CP002688 Genomic DNA Translation: AED91151.1 BT003857 mRNA Translation: AAO41907.1 |
PIRi | T49878 |
RefSeqi | NP_196356.1, NM_120821.2 [O81209-1] |
3D structure databases
SMRi | O81209 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT5G07390.1 |
Protein family/group databases
PeroxiBasei | 3282, AtRboh01 |
TCDBi | 5.B.1.1.8, the phagocyte (gp91(phox)) nadph oxidase family |
Proteomic databases
PaxDbi | O81209 |
ProteomicsDBi | 225940 [O81209-1] |
Genome annotation databases
EnsemblPlantsi | AT5G07390.1; AT5G07390.1; AT5G07390 [O81209-1] |
GeneIDi | 830630 |
Gramenei | AT5G07390.1; AT5G07390.1; AT5G07390 [O81209-1] |
KEGGi | ath:AT5G07390 |
Organism-specific databases
Araporti | AT5G07390 |
TAIRi | locus:2183309, AT5G07390 |
Phylogenomic databases
eggNOGi | KOG0039, Eukaryota |
InParanoidi | O81209 |
OMAi | SEVCNPR |
OrthoDBi | 936110at2759 |
PhylomeDBi | O81209 |
Enzyme and pathway databases
BioCyci | ARA:AT5G07390-MONOMER |
Miscellaneous databases
PROi | PR:O81209 |
Gene expression databases
ExpressionAtlasi | O81209, baseline and differential |
Genevisiblei | O81209, AT |
Family and domain databases
CDDi | cd00051, EFh, 1 hit |
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR000778, Cyt_b245_heavy_chain IPR011992, EF-hand-dom_pair IPR018247, EF_Hand_1_Ca_BS IPR002048, EF_hand_dom IPR013112, FAD-bd_8 IPR017927, FAD-bd_FR_type IPR013130, Fe3_Rdtase_TM_dom IPR013121, Fe_red_NAD-bd_6 IPR039261, FNR_nucleotide-bd IPR013623, NADPH_Ox IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF08022, FAD_binding_8, 1 hit PF01794, Ferric_reduct, 1 hit PF08030, NAD_binding_6, 1 hit PF08414, NADPH_Ox, 1 hit |
PRINTSi | PR00466, GP91PHOX |
SUPFAMi | SSF47473, SSF47473, 1 hit SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS00018, EF_HAND_1, 1 hit PS50222, EF_HAND_2, 1 hit PS51384, FAD_FR, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBOHA_ARATH | |
Accessioni | O81209Primary (citable) accession number: O81209 Secondary accession number(s): Q9LY21 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | January 15, 2008 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families