UniProtKB - O81149 (PSA5A_ARATH)
Proteasome subunit alpha type-5-A
PAE1
Functioni
The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
GO - Molecular functioni
- ribonuclease activity Source: TAIR
- structural constituent of ribosome Source: CAFA
GO - Biological processi
- proteasomal protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: InterPro
Protein family/group databases
MEROPSi | T01.995 |
Names & Taxonomyi
Protein namesi | Recommended name: Proteasome subunit alpha type-5-AAlternative name(s): 20S proteasome alpha subunit E-1 |
Gene namesi | Name:PAE1 Ordered Locus Names:At1g53850 ORF Names:T18A20.8 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G53850 |
TAIRi | locus:2197071, AT1G53850 |
Subcellular locationi
Cytosol
- cytosol Source: TAIR
- cytosolic ribosome Source: TAIR
Nucleus
- nucleus Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- cytoplasm Source: TAIR
- polysomal ribosome Source: CAFA
- proteasome complex Source: TAIR
- proteasome core complex Source: GO_Central
- proteasome core complex, alpha-subunit complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Nucleus, ProteasomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124124 | 1 – 237 | Proteasome subunit alpha type-5-AAdd BLAST | 237 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionine1 Publication | 1 | |
Cross-linki | 43 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 66 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication |
Keywords - PTMi
Acetylation, Isopeptide bond, Ubl conjugationProteomic databases
PaxDbi | O81149 |
PRIDEi | O81149 |
ProteomicsDBi | 226486 |
PTM databases
iPTMneti | O81149 |
Expressioni
Gene expression databases
ExpressionAtlasi | O81149, baseline and differential |
Genevisiblei | O81149, AT |
Interactioni
Subunit structurei
Component of the 20S core complex of the 26S proteasome. The 26S proteasome is composed of a core protease (CP), known as the 20S proteasome, capped at one or both ends by the 19S regulatory particle (RP/PA700). The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 27047, 77 interactors |
IntActi | O81149, 2 interactors |
STRINGi | 3702.AT1G53850.1 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0176, Eukaryota |
HOGENOMi | CLU_035750_4_2_1 |
InParanoidi | O81149 |
OMAi | NHYFTYN |
OrthoDBi | 1222564at2759 |
PhylomeDBi | O81149 |
Family and domain databases
CDDi | cd03753, proteasome_alpha_type_5, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR033812, Proteasome_alpha_type_5 IPR000426, Proteasome_asu_N IPR001353, Proteasome_sua/b |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MFLTRTEYDR GVNTFSPEGR LFQVEYAIEA IKLGSTAIGV KTKEGVVLAV
60 70 80 90 100
EKRITSPLLE PSSVEKIMEI DDHIGCAMSG LIADARTLVE HARVETQNHR
110 120 130 140 150
FSYGEPMTVE STTQALCDLA LRFGEGEEES MSRPFGVSLL IAGHDENGPS
160 170 180 190 200
LYYTDPSGTF WQCNAKAIGS GSEGADSSLQ EQFNKDLSLQ EAETIAVSIL
210 220 230
KQVMEEKVTP NNVDIAKVAP AYHLYTPQEV EAVIARL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 121 | L → F in BAD94476 (Ref. 6) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF043524 mRNA Translation: AAC32060.1 AC009324 Genomic DNA Translation: AAF02858.1 CP002684 Genomic DNA Translation: AEE33009.1 CP002684 Genomic DNA Translation: AEE33010.1 AY072371 mRNA Translation: AAL62363.1 AY114616 mRNA Translation: AAM47935.1 AY086045 mRNA Translation: AAM63255.1 AK220949 mRNA Translation: BAD94476.1 |
PIRi | T51972 |
RefSeqi | NP_001077717.1, NM_001084248.2 NP_175788.1, NM_104262.3 |
Genome annotation databases
EnsemblPlantsi | AT1G53850.1; AT1G53850.1; AT1G53850 AT1G53850.2; AT1G53850.2; AT1G53850 |
GeneIDi | 841822 |
Gramenei | AT1G53850.1; AT1G53850.1; AT1G53850 AT1G53850.2; AT1G53850.2; AT1G53850 |
KEGGi | ath:AT1G53850 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF043524 mRNA Translation: AAC32060.1 AC009324 Genomic DNA Translation: AAF02858.1 CP002684 Genomic DNA Translation: AEE33009.1 CP002684 Genomic DNA Translation: AEE33010.1 AY072371 mRNA Translation: AAL62363.1 AY114616 mRNA Translation: AAM47935.1 AY086045 mRNA Translation: AAM63255.1 AK220949 mRNA Translation: BAD94476.1 |
PIRi | T51972 |
RefSeqi | NP_001077717.1, NM_001084248.2 NP_175788.1, NM_104262.3 |
3D structure databases
AlphaFoldDBi | O81149 |
SMRi | O81149 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 27047, 77 interactors |
IntActi | O81149, 2 interactors |
STRINGi | 3702.AT1G53850.1 |
Protein family/group databases
MEROPSi | T01.995 |
PTM databases
iPTMneti | O81149 |
Proteomic databases
PaxDbi | O81149 |
PRIDEi | O81149 |
ProteomicsDBi | 226486 |
Genome annotation databases
EnsemblPlantsi | AT1G53850.1; AT1G53850.1; AT1G53850 AT1G53850.2; AT1G53850.2; AT1G53850 |
GeneIDi | 841822 |
Gramenei | AT1G53850.1; AT1G53850.1; AT1G53850 AT1G53850.2; AT1G53850.2; AT1G53850 |
KEGGi | ath:AT1G53850 |
Organism-specific databases
Araporti | AT1G53850 |
TAIRi | locus:2197071, AT1G53850 |
Phylogenomic databases
eggNOGi | KOG0176, Eukaryota |
HOGENOMi | CLU_035750_4_2_1 |
InParanoidi | O81149 |
OMAi | NHYFTYN |
OrthoDBi | 1222564at2759 |
PhylomeDBi | O81149 |
Miscellaneous databases
PROi | PR:O81149 |
Gene expression databases
ExpressionAtlasi | O81149, baseline and differential |
Genevisiblei | O81149, AT |
Family and domain databases
CDDi | cd03753, proteasome_alpha_type_5, 1 hit |
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR033812, Proteasome_alpha_type_5 IPR000426, Proteasome_asu_N IPR001353, Proteasome_sua/b |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PSA5A_ARATH | |
Accessioni | O81149Primary (citable) accession number: O81149 Secondary accession number(s): Q56ZL5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 8, 2000 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 163 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families