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Protein

Calcium-transporting ATPase 2, plasma membrane-type

Gene

ACA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the endoplasmic reticulum.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Activity regulationi

Activated by calmodulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4544-aspartylphosphate intermediateBy similarity1
Metal bindingi755MagnesiumBy similarity1
Metal bindingi759MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Hydrolase
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G37640-MONOMER
MetaCyc:MONOMER-14659
ReactomeiR-ATH-418359 Reduction of cytosolic Ca++ levels
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Protein family/group databases

TCDBi3.A.3.2.12 the p-type atpase (p-atpase) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 2, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 2
Gene namesi
Name:ACA2
Ordered Locus Names:At4g37640
ORF Names:F19F18.130
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G37640
TAIRilocus:2120096 AT4G37640

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 160CytoplasmicSequence analysisAdd BLAST160
Transmembranei161 – 181HelicalSequence analysisAdd BLAST21
Topological domaini182 – 199LumenalSequence analysisAdd BLAST18
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 348CytoplasmicSequence analysisAdd BLAST128
Transmembranei349 – 368HelicalSequence analysisAdd BLAST20
Topological domaini369 – 398LumenalSequence analysisAdd BLAST30
Transmembranei399 – 416HelicalSequence analysisAdd BLAST18
Topological domaini417 – 810CytoplasmicSequence analysisAdd BLAST394
Transmembranei811 – 829HelicalSequence analysisAdd BLAST19
Topological domaini830 – 840LumenalSequence analysisAdd BLAST11
Transmembranei841 – 861HelicalSequence analysisAdd BLAST21
Topological domaini862 – 881CytoplasmicSequence analysisAdd BLAST20
Transmembranei882 – 904HelicalSequence analysisAdd BLAST23
Topological domaini905 – 916LumenalSequence analysisAdd BLAST12
Transmembranei917 – 938HelicalSequence analysisAdd BLAST22
Topological domaini939 – 956CytoplasmicSequence analysisAdd BLAST18
Transmembranei957 – 978HelicalSequence analysisAdd BLAST22
Topological domaini979 – 988LumenalSequence analysis10
Transmembranei989 – 1010HelicalSequence analysisAdd BLAST22
Topological domaini1011 – 1014CytoplasmicSequence analysis4

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464111 – 1014Calcium-transporting ATPase 2, plasma membrane-typeAdd BLAST1014

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei45Phosphoserine; by CPK11 Publication1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO81108
PRIDEiO81108

PTM databases

iPTMnetiO81108

Expressioni

Gene expression databases

ExpressionAtlasiO81108 baseline and differential
GenevisibleiO81108 AT

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi15199, 6 interactors
IntActiO81108, 2 interactors
STRINGi3702.AT4G37640.1

Structurei

Secondary structure

11014
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO81108
SMRiO81108
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni20 – 31Interaction with calmodulinAdd BLAST12

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
HOGENOMiHOG000265623
InParanoidiO81108
KOiK01537
OMAiTQWIFSI
OrthoDBiEOG093600UZ
PhylomeDBiO81108

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR024750 Ca_ATPase_N_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF12515 CaATP_NAI, 1 hit
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit

Sequencei

Sequence statusi: Complete.

O81108-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MESYLNENFD VKAKHSSEEV LEKWRNLCGV VKNPKRRFRF TANLSKRYEA
60 70 80 90 100
AAMRRTNQEK LRIAVLVSKA AFQFISGVSP SDYTVPEDVK AAGFEICADE
110 120 130 140 150
LGSIVESHDV KKLKFHGGVD GLAGKLKASP TDGLSTEAAQ LSQRQELFGI
160 170 180 190 200
NKFAESEMRG FWVFVWEALQ DMTLMILGVC AFVSLIVGIA TEGWPKGSHD
210 220 230 240 250
GLGIAASILL VVFVTATSDY RQSLQFRDLD KEKKKITVQV TRNGFRQKLS
260 270 280 290 300
IYDLLPGDIV HLAIGDQVPA DGLFLSGFSV VIDESSLTGE SEPVMVNAQN
310 320 330 340 350
PFLMSGTKVQ DGSCKMMITT VGMRTQWGKL MATLTEGGDD ETPLQVKLNG
360 370 380 390 400
VATIIGKIGL FFAVVTFAVL VQGMFMRKLS TGTHWVWSGD EALELLEYFA
410 420 430 440 450
IAVTIVVVAV PEGLPLAVTL SLAFAMKKMM NDKALVRHLA ACETMGSATT
460 470 480 490 500
ICSDKTGTLT TNHMTVVKSC ICMNVQDVAN KGSSLQSEIP ESAVKLLIQS
510 520 530 540 550
IFNNTGGEVV VNKHGKTELL GTPTETAILE LGLSLGGKFQ EERKSYKVIK
560 570 580 590 600
VEPFNSTKKR MGVVIELPEG GRMRAHTKGA SEIVLAACDK VVNSSGEVVP
610 620 630 640 650
LDEESIKYLN VTINEFANEA LRTLCLAYMD IEGGFSPDDA IPASGFTCVG
660 670 680 690 700
IVGIKDPVRP GVKESVELCR RAGITVRMVT GDNINTAKAI ARECGILTDD
710 720 730 740 750
GIAIEGPVFR EKNQEELLEL IPKIQVMARS SPMDKHTLVK QLRTTFDEVV
760 770 780 790 800
AVTGDGTNDA PALHEADIGL AMGIAGTEVA KESADVIILD DNFSTIVTVA
810 820 830 840 850
KWGRSVYINI QKFVQFQLTV NVVALVVNFS SACLTGSAPL TAVQLLWVNM
860 870 880 890 900
IMDTLGALAL ATEPPNDELM KRLPVGRRGN FITNAMWRNI LGQAVYQFIV
910 920 930 940 950
IWILQAKGKA MFGLDGPDST LMLNTLIFNC FVFCQVFNEI SSREMEEIDV
960 970 980 990 1000
FKGILDNYVF VVVIGATVFF QIIIIEFLGT FASTTPLTIT QWIFSIFIGF
1010
LGMPIAAGLK TIPV
Length:1,014
Mass (Da):110,439
Last modified:November 1, 1998 - v1
Checksum:i996DEFD26AFE9F03
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025842 mRNA Translation: AAC26997.1
AL035605 Genomic DNA Translation: CAB38303.1
AL161591 Genomic DNA Translation: CAB80429.1
CP002687 Genomic DNA Translation: AEE86819.1
AY062484 mRNA Translation: AAL32562.1
PIRiT04721
RefSeqiNP_195479.1, NM_119927.3
UniGeneiAt.24252

Genome annotation databases

EnsemblPlantsiAT4G37640.1; AT4G37640.1; AT4G37640
GeneIDi829918
GrameneiAT4G37640.1; AT4G37640.1; AT4G37640
KEGGiath:AT4G37640

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025842 mRNA Translation: AAC26997.1
AL035605 Genomic DNA Translation: CAB38303.1
AL161591 Genomic DNA Translation: CAB80429.1
CP002687 Genomic DNA Translation: AEE86819.1
AY062484 mRNA Translation: AAL32562.1
PIRiT04721
RefSeqiNP_195479.1, NM_119927.3
UniGeneiAt.24252

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2M7ENMR-A20-45[»]
ProteinModelPortaliO81108
SMRiO81108
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15199, 6 interactors
IntActiO81108, 2 interactors
STRINGi3702.AT4G37640.1

Protein family/group databases

TCDBi3.A.3.2.12 the p-type atpase (p-atpase) superfamily

PTM databases

iPTMnetiO81108

Proteomic databases

PaxDbiO81108
PRIDEiO81108

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G37640.1; AT4G37640.1; AT4G37640
GeneIDi829918
GrameneiAT4G37640.1; AT4G37640.1; AT4G37640
KEGGiath:AT4G37640

Organism-specific databases

AraportiAT4G37640
TAIRilocus:2120096 AT4G37640

Phylogenomic databases

eggNOGiKOG0204 Eukaryota
ENOG410XNNC LUCA
HOGENOMiHOG000265623
InParanoidiO81108
KOiK01537
OMAiTQWIFSI
OrthoDBiEOG093600UZ
PhylomeDBiO81108

Enzyme and pathway databases

BioCyciARA:AT4G37640-MONOMER
MetaCyc:MONOMER-14659
ReactomeiR-ATH-418359 Reduction of cytosolic Ca++ levels
R-ATH-5578775 Ion homeostasis
R-ATH-936837 Ion transport by P-type ATPases

Miscellaneous databases

PROiPR:O81108

Gene expression databases

ExpressionAtlasiO81108 baseline and differential
GenevisibleiO81108 AT

Family and domain databases

Gene3Di3.40.1110.10, 1 hit
3.40.50.1000, 1 hit
InterProiView protein in InterPro
IPR006068 ATPase_P-typ_cation-transptr_C
IPR004014 ATPase_P-typ_cation-transptr_N
IPR023299 ATPase_P-typ_cyto_dom_N
IPR018303 ATPase_P-typ_P_site
IPR023298 ATPase_P-typ_TM_dom_sf
IPR008250 ATPase_P-typ_transduc_dom_A_sf
IPR024750 Ca_ATPase_N_dom
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006408 P-type_ATPase_IIB
IPR001757 P_typ_ATPase
PfamiView protein in Pfam
PF12515 CaATP_NAI, 1 hit
PF00689 Cation_ATPase_C, 1 hit
PF00690 Cation_ATPase_N, 1 hit
PRINTSiPR00120 HATPASE
SMARTiView protein in SMART
SM00831 Cation_ATPase_N, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
SSF81653 SSF81653, 1 hit
SSF81660 SSF81660, 1 hit
SSF81665 SSF81665, 1 hit
TIGRFAMsiTIGR01517 ATPase-IIB_Ca, 1 hit
TIGR01494 ATPase_P-type, 3 hits
PROSITEiView protein in PROSITE
PS00154 ATPASE_E1_E2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACA2_ARATH
AccessioniPrimary (citable) accession number: O81108
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: November 1, 1998
Last modified: November 7, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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