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UniProtKB - O81108 (ACA2_ARATH)
Protein
Calcium-transporting ATPase 2, plasma membrane-type
Gene
ACA2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the endoplasmic reticulum.
Catalytic activityi
- EC:7.2.2.10
Activity regulationi
Activated by calmodulin.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 454 | 4-aspartylphosphate intermediateBy similarity | 1 | |
Metal bindingi | 755 | MagnesiumBy similarity | 1 | |
Metal bindingi | 759 | MagnesiumBy similarity | 1 |
GO - Molecular functioni
- ATPase-coupled cation transmembrane transporter activity Source: GO_Central
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- calcium ion transmembrane transporter activity Source: TAIR
- calmodulin binding Source: TAIR
- metal ion binding Source: UniProtKB-KW
- P-type calcium transporter activity Source: TAIR
GO - Biological processi
- calcium ion transmembrane transport Source: TAIR
Keywordsi
Molecular function | Calmodulin-binding, Translocase |
Biological process | Calcium transport, Ion transport, Transport |
Ligand | ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT4G37640-MONOMER |
Protein family/group databases
TCDBi | 3.A.3.2.12, the p-type atpase (p-atpase) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Calcium-transporting ATPase 2, plasma membrane-type (EC:7.2.2.10)Alternative name(s): Ca(2+)-ATPase isoform 2 |
Gene namesi | Name:ACA2 Ordered Locus Names:At4g37640 ORF Names:F19F18.130 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G37640 |
TAIRi | locus:2120096, AT4G37640 |
Subcellular locationi
Endoplasmic reticulum
Endoplasmic reticulum
- endoplasmic reticulum Source: TAIR
- endoplasmic reticulum membrane Source: TAIR
Plasma Membrane
- integral component of plasma membrane Source: GO_Central
Other locations
- intracellular membrane-bounded organelle Source: GO_Central
- membrane Source: TAIR
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 160 | CytoplasmicSequence analysisAdd BLAST | 160 | |
Transmembranei | 161 – 181 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 182 – 199 | LumenalSequence analysisAdd BLAST | 18 | |
Transmembranei | 200 – 220 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 221 – 348 | CytoplasmicSequence analysisAdd BLAST | 128 | |
Transmembranei | 349 – 368 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 369 – 398 | LumenalSequence analysisAdd BLAST | 30 | |
Transmembranei | 399 – 416 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 417 – 810 | CytoplasmicSequence analysisAdd BLAST | 394 | |
Transmembranei | 811 – 829 | HelicalSequence analysisAdd BLAST | 19 | |
Topological domaini | 830 – 840 | LumenalSequence analysisAdd BLAST | 11 | |
Transmembranei | 841 – 861 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 862 – 881 | CytoplasmicSequence analysisAdd BLAST | 20 | |
Transmembranei | 882 – 904 | HelicalSequence analysisAdd BLAST | 23 | |
Topological domaini | 905 – 916 | LumenalSequence analysisAdd BLAST | 12 | |
Transmembranei | 917 – 938 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 939 – 956 | CytoplasmicSequence analysisAdd BLAST | 18 | |
Transmembranei | 957 – 978 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 979 – 988 | LumenalSequence analysis | 10 | |
Transmembranei | 989 – 1010 | HelicalSequence analysisAdd BLAST | 22 | |
Topological domaini | 1011 – 1014 | CytoplasmicSequence analysis | 4 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046411 | 1 – 1014 | Calcium-transporting ATPase 2, plasma membrane-typeAdd BLAST | 1014 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 | |
Modified residuei | 45 | Phosphoserine; by CPK11 Publication | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | O81108 |
PRIDEi | O81108 |
ProteomicsDBi | 244631 |
PTM databases
iPTMneti | O81108 |
Expressioni
Gene expression databases
ExpressionAtlasi | O81108, baseline and differential |
Genevisiblei | O81108, AT |
Interactioni
GO - Molecular functioni
- calmodulin binding Source: TAIR
Protein-protein interaction databases
BioGRIDi | 15199, 6 interactors |
IntActi | O81108, 2 interactors |
STRINGi | 3702.AT4G37640.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | O81108 |
BMRBi | O81108 |
SMRi | O81108 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 20 – 31 | Interaction with calmodulinAdd BLAST | 12 |
Domaini
The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.
Sequence similaritiesi
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0204, Eukaryota |
HOGENOMi | CLU_002360_9_2_1 |
InParanoidi | O81108 |
OMAi | GECLVIA |
OrthoDBi | 115892at2759 |
PhylomeDBi | O81108 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR024750, Ca_ATPase_N_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006408, P-type_ATPase_IIB IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF12515, CaATP_NAI, 1 hit PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01517, ATPase-IIB_Ca, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
i Sequence
Sequence statusi: Complete.
O81108-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MESYLNENFD VKAKHSSEEV LEKWRNLCGV VKNPKRRFRF TANLSKRYEA
60 70 80 90 100
AAMRRTNQEK LRIAVLVSKA AFQFISGVSP SDYTVPEDVK AAGFEICADE
110 120 130 140 150
LGSIVESHDV KKLKFHGGVD GLAGKLKASP TDGLSTEAAQ LSQRQELFGI
160 170 180 190 200
NKFAESEMRG FWVFVWEALQ DMTLMILGVC AFVSLIVGIA TEGWPKGSHD
210 220 230 240 250
GLGIAASILL VVFVTATSDY RQSLQFRDLD KEKKKITVQV TRNGFRQKLS
260 270 280 290 300
IYDLLPGDIV HLAIGDQVPA DGLFLSGFSV VIDESSLTGE SEPVMVNAQN
310 320 330 340 350
PFLMSGTKVQ DGSCKMMITT VGMRTQWGKL MATLTEGGDD ETPLQVKLNG
360 370 380 390 400
VATIIGKIGL FFAVVTFAVL VQGMFMRKLS TGTHWVWSGD EALELLEYFA
410 420 430 440 450
IAVTIVVVAV PEGLPLAVTL SLAFAMKKMM NDKALVRHLA ACETMGSATT
460 470 480 490 500
ICSDKTGTLT TNHMTVVKSC ICMNVQDVAN KGSSLQSEIP ESAVKLLIQS
510 520 530 540 550
IFNNTGGEVV VNKHGKTELL GTPTETAILE LGLSLGGKFQ EERKSYKVIK
560 570 580 590 600
VEPFNSTKKR MGVVIELPEG GRMRAHTKGA SEIVLAACDK VVNSSGEVVP
610 620 630 640 650
LDEESIKYLN VTINEFANEA LRTLCLAYMD IEGGFSPDDA IPASGFTCVG
660 670 680 690 700
IVGIKDPVRP GVKESVELCR RAGITVRMVT GDNINTAKAI ARECGILTDD
710 720 730 740 750
GIAIEGPVFR EKNQEELLEL IPKIQVMARS SPMDKHTLVK QLRTTFDEVV
760 770 780 790 800
AVTGDGTNDA PALHEADIGL AMGIAGTEVA KESADVIILD DNFSTIVTVA
810 820 830 840 850
KWGRSVYINI QKFVQFQLTV NVVALVVNFS SACLTGSAPL TAVQLLWVNM
860 870 880 890 900
IMDTLGALAL ATEPPNDELM KRLPVGRRGN FITNAMWRNI LGQAVYQFIV
910 920 930 940 950
IWILQAKGKA MFGLDGPDST LMLNTLIFNC FVFCQVFNEI SSREMEEIDV
960 970 980 990 1000
FKGILDNYVF VVVIGATVFF QIIIIEFLGT FASTTPLTIT QWIFSIFIGF
1010
LGMPIAAGLK TIPV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF025842 mRNA Translation: AAC26997.1 AL035605 Genomic DNA Translation: CAB38303.1 AL161591 Genomic DNA Translation: CAB80429.1 CP002687 Genomic DNA Translation: AEE86819.1 AY062484 mRNA Translation: AAL32562.1 |
PIRi | T04721 |
RefSeqi | NP_195479.1, NM_119927.3 |
Genome annotation databases
EnsemblPlantsi | AT4G37640.1; AT4G37640.1; AT4G37640 |
GeneIDi | 829918 |
Gramenei | AT4G37640.1; AT4G37640.1; AT4G37640 |
KEGGi | ath:AT4G37640 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF025842 mRNA Translation: AAC26997.1 AL035605 Genomic DNA Translation: CAB38303.1 AL161591 Genomic DNA Translation: CAB80429.1 CP002687 Genomic DNA Translation: AEE86819.1 AY062484 mRNA Translation: AAL32562.1 |
PIRi | T04721 |
RefSeqi | NP_195479.1, NM_119927.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2M7E | NMR | - | A | 20-45 | [»] | |
AlphaFoldDBi | O81108 | |||||
BMRBi | O81108 | |||||
SMRi | O81108 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 15199, 6 interactors |
IntActi | O81108, 2 interactors |
STRINGi | 3702.AT4G37640.1 |
Protein family/group databases
TCDBi | 3.A.3.2.12, the p-type atpase (p-atpase) superfamily |
PTM databases
iPTMneti | O81108 |
Proteomic databases
PaxDbi | O81108 |
PRIDEi | O81108 |
ProteomicsDBi | 244631 |
Genome annotation databases
EnsemblPlantsi | AT4G37640.1; AT4G37640.1; AT4G37640 |
GeneIDi | 829918 |
Gramenei | AT4G37640.1; AT4G37640.1; AT4G37640 |
KEGGi | ath:AT4G37640 |
Organism-specific databases
Araporti | AT4G37640 |
TAIRi | locus:2120096, AT4G37640 |
Phylogenomic databases
eggNOGi | KOG0204, Eukaryota |
HOGENOMi | CLU_002360_9_2_1 |
InParanoidi | O81108 |
OMAi | GECLVIA |
OrthoDBi | 115892at2759 |
PhylomeDBi | O81108 |
Enzyme and pathway databases
BioCyci | ARA:AT4G37640-MONOMER |
Miscellaneous databases
PROi | PR:O81108 |
Gene expression databases
ExpressionAtlasi | O81108, baseline and differential |
Genevisiblei | O81108, AT |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR006068, ATPase_P-typ_cation-transptr_C IPR004014, ATPase_P-typ_cation-transptr_N IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR024750, Ca_ATPase_N_dom IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006408, P-type_ATPase_IIB IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
Pfami | View protein in Pfam PF12515, CaATP_NAI, 1 hit PF00689, Cation_ATPase_C, 1 hit PF00690, Cation_ATPase_N, 1 hit |
PRINTSi | PR00120, HATPASE |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SMARTi | View protein in SMART SM00831, Cation_ATPase_N, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01517, ATPase-IIB_Ca, 1 hit TIGR01494, ATPase_P-type, 3 hits |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ACA2_ARATH | |
Accessioni | O81108Primary (citable) accession number: O81108 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 11, 2001 |
Last sequence update: | November 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 190 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families