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Protein

Heat stress transcription factor A-2

Gene

HSFA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE). Involved in heat stress responses. Seems to be involved in other environmental stress responses. Activates ascorbate peroxidase 2 (APX2) in addition to several heat shock protein (HSPs).3 Publications

Miscellaneous

Plants overexpressing HSFA2 show increased tolerance to combined environmental stresses.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi42 – 136By similarityAdd BLAST95

GO - Molecular functioni

  • DNA-binding transcription factor activity Source: TAIR
  • sequence-specific DNA binding Source: InterPro
  • transcription regulatory region DNA binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371511 HSF1 activation
R-ATH-3371568 Attenuation phase
R-ATH-3371571 HSF1-dependent transactivation

Names & Taxonomyi

Protein namesi
Recommended name:
Heat stress transcription factor A-2
Short name:
AtHsfA2
Alternative name(s):
AtHsf-04
Gene namesi
Name:HSFA2
Synonyms:HSF04
Ordered Locus Names:At2g26150
ORF Names:T19L18.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G26150
TAIRilocus:2057371 AT2G26150

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Heat-sensitive phenotype.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5K → R: No effect on SUMO1 binding. 1 Publication1
Mutagenesisi167K → R: Reduces SUMO1 binding efficiency. 1 Publication1
Mutagenesisi269K → R: Increases SUMO1 binding efficiency. 1 Publication1
Mutagenesisi315K → R: Loss of sumoylation. Loss of SUMO1 binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002708021 – 345Heat stress transcription factor A-2Add BLAST345

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki315Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication

Post-translational modificationi

Exhibits temperature-dependent phosphorylation.By similarity
Sumoylated at Lys-315. Sumoylation represses its function.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO80982

PTM databases

iPTMnetiO80982

Expressioni

Inductioni

By heat stress, high light and hydrogen peroxide (H2O2).3 Publications

Gene expression databases

ExpressionAtlasiO80982 baseline and differential
GenevisibleiO80982 AT

Interactioni

Subunit structurei

Homotrimer (By similarity). Interacts with SUMO1 (PubMed:20521085). Binds to HSBP (PubMed:20388662).By similarity2 Publications

Protein-protein interaction databases

BioGridi2507, 6 interactors
IntActiO80982, 2 interactors
MINTiO80982
STRINGi3702.AT2G26150.1

Structurei

3D structure databases

ProteinModelPortaliO80982
SMRiO80982
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni150 – 216Hydrophobic repeat HR-A/BAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi230 – 245Bipartite nuclear localization signalSequence analysisAdd BLAST16
Motifi273 – 282AHA110
Motifi324 – 333AHA210
Motifi334 – 341Nuclear export signalSequence analysis8

Domaini

The hydrophobic-rich region (HR-A/B) corresponds to the oligomerization domain. AHA motifs are transcriptional activator elements.1 Publication

Sequence similaritiesi

Belongs to the HSF family. Class A subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0627 Eukaryota
COG5169 LUCA
HOGENOMiHOG000237979
InParanoidiO80982
KOiK09419
OMAiDQEFDRM
OrthoDBiEOG09360BUI
PhylomeDBiO80982

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 1 hit
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket
Note: A number of isoforms are produced. According to EST sequences.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O80982-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEELKVEMEE ETVTFTGSVA ASSSVGSSSS PRPMEGLNET GPPPFLTKTY
60 70 80 90 100
EMVEDPATDT VVSWSNGRNS FVVWDSHKFS TTLLPRYFKH SNFSSFIRQL
110 120 130 140 150
NTYGFRKIDP DRWEFANEGF LAGQKHLLKN IKRRRNMGLQ NVNQQGSGMS
160 170 180 190 200
CVEVGQYGFD GEVERLKRDH GVLVAEVVRL RQQQHSSKSQ VAAMEQRLLV
210 220 230 240 250
TEKRQQQMMT FLAKALNNPN FVQQFAVMSK EKKSLFGLDV GRKRRLTSTP
260 270 280 290 300
SLGTMEENLL HDQEFDRMKD DMEMLFAAAI DDEANNSMPT KEEQCLEAMN
310 320 330 340
VMMRDGNLEA ALDVKVEDLV GSPLDWDSQD LHDMVDQMGF LGSEP
Length:345
Mass (Da):39,114
Last modified:November 1, 1998 - v1
Checksum:i455FAB34582D7150
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B3H5P6B3H5P6_ARATH
Heat shock transcription factor A2
HSFA2 ATHSFA2, At2g26150, T19L18.4, T19L18_4
290Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004747 Genomic DNA Translation: AAC31222.1
CP002685 Genomic DNA Translation: AEC07800.1
CP002685 Genomic DNA Translation: ANM62674.1
AK118744 mRNA Translation: BAC43337.1
PIRiT02609
RefSeqiNP_001324815.1, NM_001336039.1 [O80982-1]
NP_180184.1, NM_128173.3 [O80982-1]
UniGeneiAt.38933

Genome annotation databases

EnsemblPlantsiAT2G26150.1; AT2G26150.1; AT2G26150 [O80982-1]
AT2G26150.4; AT2G26150.4; AT2G26150 [O80982-1]
GeneIDi817155
GrameneiAT2G26150.1; AT2G26150.1; AT2G26150 [O80982-1]
AT2G26150.4; AT2G26150.4; AT2G26150 [O80982-1]
KEGGiath:AT2G26150

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC004747 Genomic DNA Translation: AAC31222.1
CP002685 Genomic DNA Translation: AEC07800.1
CP002685 Genomic DNA Translation: ANM62674.1
AK118744 mRNA Translation: BAC43337.1
PIRiT02609
RefSeqiNP_001324815.1, NM_001336039.1 [O80982-1]
NP_180184.1, NM_128173.3 [O80982-1]
UniGeneiAt.38933

3D structure databases

ProteinModelPortaliO80982
SMRiO80982
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2507, 6 interactors
IntActiO80982, 2 interactors
MINTiO80982
STRINGi3702.AT2G26150.1

PTM databases

iPTMnetiO80982

Proteomic databases

PaxDbiO80982

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G26150.1; AT2G26150.1; AT2G26150 [O80982-1]
AT2G26150.4; AT2G26150.4; AT2G26150 [O80982-1]
GeneIDi817155
GrameneiAT2G26150.1; AT2G26150.1; AT2G26150 [O80982-1]
AT2G26150.4; AT2G26150.4; AT2G26150 [O80982-1]
KEGGiath:AT2G26150

Organism-specific databases

AraportiAT2G26150
TAIRilocus:2057371 AT2G26150

Phylogenomic databases

eggNOGiKOG0627 Eukaryota
COG5169 LUCA
HOGENOMiHOG000237979
InParanoidiO80982
KOiK09419
OMAiDQEFDRM
OrthoDBiEOG09360BUI
PhylomeDBiO80982

Enzyme and pathway databases

ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371511 HSF1 activation
R-ATH-3371568 Attenuation phase
R-ATH-3371571 HSF1-dependent transactivation

Miscellaneous databases

PROiPR:O80982

Gene expression databases

ExpressionAtlasiO80982 baseline and differential
GenevisibleiO80982 AT

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 1 hit
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHSFA2_ARATH
AccessioniPrimary (citable) accession number: O80982
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: November 1, 1998
Last modified: September 12, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
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Main funding by: National Institutes of Health

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